Reviewed,
UniProtKB/Swiss-Prot Q14451 (GRB7_HUMAN)
Last modified
November 25, 2008.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Growth factor receptor-bound protein 7 Alternative name(s): GRB7 adapter protein Epidermal growth factor receptor GRB-7 B47 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 532 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. |
| Subunit structure | Interacts with RND1. |
| Post-translational modification | Phosphorylated on tyrosine residues. |
| Sequence similarities | Belongs to the GRB7/10/14 family. Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 SH2 domain. |
Ontologies
Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Domain | SH2 domain |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | epidermal growth factor receptor signaling pathway Ref.2 Traceable author statement. Source: ProtInc |
| Molecular function | SH3/SH2 adaptor activity Ref.2 Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CALM | P62157 | 1 | EBI-970191,EBI-397403 | From a different organism. |
| Calm1 | P62161 | 1 | EBI-970191,EBI-397530 | From a different organism. |
| RND1 | Q92730 | 2 | EBI-970191,EBI-448618 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] Notes: At least 2 isoforms are produced. | ||||||
| Isoform 1 (identifier: Q14451-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q14451-2) Also known as: Grb7V; The sequence of this isoform differs from the canonical sequence as follows: 425-447: IHRTQLWFHGRISREESQRLIGQ → CSWSGRVSGTPRALSATCRK 448-532: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 532 | 532 | Growth factor receptor-bound protein 7 | PRO_0000150344 | ||||||||||||||||||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Domain | 100 – 186 | 87 | Ras-associating | |||||||||||||||||||||||||||||||||||||||||||||||
| Domain | 229 – 338 | 110 | PH | |||||||||||||||||||||||||||||||||||||||||||||||
| Domain | 431 – 527 | 97 | SH2 | |||||||||||||||||||||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 297 | 1 | Phosphothreonine By similarity | |||||||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 361 | 1 | Phosphoserine | |||||||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 366 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative sequence | 425 – 447 | 23 | IHRTQ…RLIGQ → CSWSGRVSGTPRALSATCRK in isoform 2. | VSP_035500 | ||||||||||||||||||||||||||||||||||||||||||||||
| Alternative sequence | 448 – 532 | 85 | Missing in isoform 2. | VSP_035501 | ||||||||||||||||||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 18 | 1 | C → W Ref.1 Ref.2 Ref.3 | |||||||||||||||||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 102 – 108 | 7 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 113 – 118 | 6 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 124 – 132 | 9 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 134 – 136 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 145 – 148 | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Turn | 150 – 152 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 155 – 158 | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 160 – 162 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 164 – 168 | 5 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 173 – 175 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 178 – 181 | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 427 – 429 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 432 – 434 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 438 – 444 | 7 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 445 – 447 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 456 – 459 | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 461 – 463 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 467 – 472 | 6 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 477 – 483 | 7 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 505 – 511 | 7 | ||||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 513 – 515 | 3 | ||||||||||||||||||||||||||||||||||||||||||||||||
Sequences
| ||||||||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of human GRB-7 co-amplified with CAB1 and c-ERBB-2 in primary gastric cancer." Kishi T., Sasaki H., Akiyama N., Ishizuka T., Sakamoto H., Aizawa S., Sugimura T., Terada M. Biochem. Biophys. Res. Commun. 232:5-9(1997) [PubMed: 9125150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Oesophageal carcinoma. |
| [2] | "A novel variant of human Grb7 is associated with invasive esophageal carcinoma." Tanaka S., Mori M., Akiyoshi T., Tanaka Y., Mafune K., Wands J.R., Sugimachi K. J. Clin. Invest. 102:821-827(1998) [PubMed: 9710451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Genomic organization and amplification of the human GRB7 gene." Whittock N.V., Eady R.A.J., McGrath J.A. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung. |
| [5] | "Coexpression of Grb7 with epidermal growth factor receptor or Her2/erbB2 in human advanced esophageal carcinoma." Tanaka S., Mori M., Akiyoshi T., Tanaka Y., Mafune K., Wands J.R., Sugimachi K. Cancer Res. 57:28-31(1997) [PubMed: 8988034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 130-343. |
| [6] | "Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family." Vayssiere B., Zalcman G., Mahe Y., Mirey G., Ligensa T., Weidner K.M., Chardin P., Camonis J. FEBS Lett. 467:91-96(2000) [PubMed: 10664463] [Abstract] Cited for: INTERACTION WITH RND1. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, MASS SPECTROMETRY. |
| [8] | "Solution structure of the RA domain of human GRB7 protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 100-186. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D43772 mRNA. Translation: BAA07827.1. AB008789 mRNA. Translation: BAA29059.1. AB008790 mRNA. Translation: BAA29060.1. AF274875 Genomic DNA. Translation: AAG25938.1. BC006535 mRNA. Translation: AAH06535.1. D87513 mRNA. Translation: BAA13412.1. | |||||||||||||||||||
| PIR | JC5412. | ||||||||||||||||||
| RefSeq | NP_001025173.1. NP_005301.2. | ||||||||||||||||||
| UniGene | Hs.86859 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP:502N. | ||||||||||||||||||
| IntAct | Q14451. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | Q14451. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSG00000141738. Homo sapiens. [Contig view] | ||||||||||||||||||
| GeneID | 2886. | ||||||||||||||||||
| KEGG | hsa:2886. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| H-InvDB | HIX0013788. | ||||||||||||||||||
| HGNC | HGNC:4567. GRB7. | ||||||||||||||||||
| HPA | CAB005226. | ||||||||||||||||||
| MIM | 601522. gene. | ||||||||||||||||||
| PharmGKB | PA28963. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | Q14451. | ||||||||||||||||||
| HOVERGEN | Q14451. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | Q14451. | ||||||||||||||||||
| CleanEx | HS_GRB7. | ||||||||||||||||||
| GermOnline | ENSG00000141738. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR015042. BPS. IPR001849. PH. IPR011993. PH_type. IPR000159. Ras-assoc. IPR000980. SH2. [Graphical view] | ||||||||||||||||||
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit. | ||||||||||||||||||
| Pfam | PF08947. BPS. 1 hit. PF00169. PH. 1 hit. PF00788. RA. 1 hit. PF00017. SH2. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00401. SH2DOMAIN. | ||||||||||||||||||
| ProDom | PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||
| SMART | SM00233. PH. 1 hit. SM00314. RA. 1 hit. SM00252. SH2. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS50003. PH_DOMAIN. 1 hit. PS50200. RA. 1 hit. PS50001. SH2. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 11395. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | GRB7_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q14451 Secondary accession number(s): Q92568, Q96DF9, Q9Y220 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


