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Q14442

- PIGH_HUMAN

UniProt

Q14442 - PIGH_HUMAN

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Protein
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H
Gene
PIGH
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

Pathwayi

GO - Molecular functioni

  1. catalytic activity Source: ProtInc
  2. phosphatidylinositol N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. C-terminal protein lipidation Source: Reactome
  2. GPI anchor biosynthetic process Source: ProtInc
  3. cellular protein metabolic process Source: Reactome
  4. cellular protein modification process Source: ProtInc
  5. post-translational protein modification Source: Reactome
  6. preassembly of GPI anchor in ER membrane Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_952. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H (EC:2.4.1.198)
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class H protein
Short name:
PIG-H
Gene namesi
Name:PIGH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:8964. PIGH.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: ProtInc
  2. endoplasmic reticulum membrane Source: Reactome
  3. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33295.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Phosphatidylinositol N-acetylglucosaminyltransferase subunit H
PRO_0000058435Add
BLAST

Proteomic databases

MaxQBiQ14442.
PaxDbiQ14442.
PRIDEiQ14442.

PTM databases

PhosphoSiteiQ14442.

Expressioni

Gene expression databases

ArrayExpressiQ14442.
BgeeiQ14442.
CleanExiHS_PIGH.
GenevestigatoriQ14442.

Organism-specific databases

HPAiHPA031624.

Interactioni

Subunit structurei

Associates with PIGA, PIGC, PIGP, PIGQ and DPM2. The latter is not essential for activity.

Protein-protein interaction databases

BioGridi111301. 5 interactions.
IntActiQ14442. 1 interaction.
STRINGi9606.ENSP00000216452.

Structurei

3D structure databases

ProteinModelPortaliQ14442.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGH family.

Phylogenomic databases

eggNOGiNOG266799.
HOGENOMiHOG000059602.
HOVERGENiHBG025632.
InParanoidiQ14442.
KOiK03858.
OMAiTKRICRC.
PhylomeDBiQ14442.
TreeFamiTF324479.

Family and domain databases

InterProiIPR019328. GPI-GlcNAc_Trfase_PIG-H_dom.
[Graphical view]
PfamiPF10181. PIG-H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q14442-1 [UniParc]FASTAAdd to Basket

« Hide

MEDERSFSDI CGGRLALQRR YYSPSCREFC LSCPRLSLRS LTAVTCTVWL    50
AAYGLFTLCE NSMILSAAIF ITLLGLLGYL HFVKIDQETL LIIDSLGIQM 100
TSSYASGKES TTFIEMGKVK DIVINEAIYM QKVIYYLCIL LKDPVEPHGI 150
SQVVPVFQSA KPRLDCLIEV YRSCQEILAH QKATSTSP 188
Length:188
Mass (Da):21,081
Last modified:November 1, 1996 - v1
Checksum:iEE70E4156272AC8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19783 mRNA. Translation: AAA03545.1.
BT006804 mRNA. Translation: AAP35450.1.
AK314108 mRNA. Translation: BAG36801.1.
CH471061 Genomic DNA. Translation: EAW80942.1.
BC004100 mRNA. Translation: AAH04100.1.
BC071849 mRNA. Translation: AAH71849.1.
CCDSiCCDS9784.1.
PIRiA48024.
RefSeqiNP_004560.1. NM_004569.3.
UniGeneiHs.553497.

Genome annotation databases

EnsembliENST00000216452; ENSP00000216452; ENSG00000100564.
GeneIDi5283.
KEGGihsa:5283.
UCSCiuc001xjr.1. human.

Polymorphism databases

DMDMi27151659.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Phosphatidylinositol N-acetylglucosaminyltransferase subunit H

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19783 mRNA. Translation: AAA03545.1 .
BT006804 mRNA. Translation: AAP35450.1 .
AK314108 mRNA. Translation: BAG36801.1 .
CH471061 Genomic DNA. Translation: EAW80942.1 .
BC004100 mRNA. Translation: AAH04100.1 .
BC071849 mRNA. Translation: AAH71849.1 .
CCDSi CCDS9784.1.
PIRi A48024.
RefSeqi NP_004560.1. NM_004569.3.
UniGenei Hs.553497.

3D structure databases

ProteinModelPortali Q14442.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111301. 5 interactions.
IntActi Q14442. 1 interaction.
STRINGi 9606.ENSP00000216452.

PTM databases

PhosphoSitei Q14442.

Polymorphism databases

DMDMi 27151659.

Proteomic databases

MaxQBi Q14442.
PaxDbi Q14442.
PRIDEi Q14442.

Protocols and materials databases

DNASUi 5283.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000216452 ; ENSP00000216452 ; ENSG00000100564 .
GeneIDi 5283.
KEGGi hsa:5283.
UCSCi uc001xjr.1. human.

Organism-specific databases

CTDi 5283.
GeneCardsi GC14M068056.
HGNCi HGNC:8964. PIGH.
HPAi HPA031624.
MIMi 600154. gene.
neXtProti NX_Q14442.
PharmGKBi PA33295.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG266799.
HOGENOMi HOG000059602.
HOVERGENi HBG025632.
InParanoidi Q14442.
KOi K03858.
OMAi TKRICRC.
PhylomeDBi Q14442.
TreeFami TF324479.

Enzyme and pathway databases

UniPathwayi UPA00196 .
Reactomei REACT_952. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

GeneWikii PIGH.
GenomeRNAii 5283.
NextBioi 20416.
PROi Q14442.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q14442.
Bgeei Q14442.
CleanExi HS_PIGH.
Genevestigatori Q14442.

Family and domain databases

InterProi IPR019328. GPI-GlcNAc_Trfase_PIG-H_dom.
[Graphical view ]
Pfami PF10181. PIG-H. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone."
    Kamitani T., Chang H.M., Rollins C., Waneck G.L., Yeh E.T.
    J. Biol. Chem. 268:20733-20736(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Lung.
  6. "The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1."
    Watanabe R., Inoue N., Westfall B., Taron C.H., Orlean P., Takeda J., Kinoshita T.
    EMBO J. 17:877-885(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIGA; PIGC AND PIGQ.
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiPIGH_HUMAN
AccessioniPrimary (citable) accession number: Q14442
Secondary accession number(s): B2RAA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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