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Q14442

- PIGH_HUMAN

UniProt

Q14442 - PIGH_HUMAN

Protein

Phosphatidylinositol N-acetylglucosaminyltransferase subunit H

Gene

PIGH

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

    Pathwayi

    GO - Molecular functioni

    1. catalytic activity Source: ProtInc
    2. phosphatidylinositol N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. cellular protein modification process Source: ProtInc
    3. C-terminal protein lipidation Source: Reactome
    4. GPI anchor biosynthetic process Source: ProtInc
    5. post-translational protein modification Source: Reactome
    6. preassembly of GPI anchor in ER membrane Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_952. Synthesis of glycosylphosphatidylinositol (GPI).
    UniPathwayiUPA00196.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase subunit H (EC:2.4.1.198)
    Alternative name(s):
    Phosphatidylinositol-glycan biosynthesis class H protein
    Short name:
    PIG-H
    Gene namesi
    Name:PIGH
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:8964. PIGH.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: ProtInc
    2. endoplasmic reticulum membrane Source: Reactome
    3. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33295.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Phosphatidylinositol N-acetylglucosaminyltransferase subunit HPRO_0000058435Add
    BLAST

    Proteomic databases

    MaxQBiQ14442.
    PaxDbiQ14442.
    PRIDEiQ14442.

    PTM databases

    PhosphoSiteiQ14442.

    Expressioni

    Gene expression databases

    ArrayExpressiQ14442.
    BgeeiQ14442.
    CleanExiHS_PIGH.
    GenevestigatoriQ14442.

    Organism-specific databases

    HPAiHPA031624.

    Interactioni

    Subunit structurei

    Associates with PIGA, PIGC, PIGP, PIGQ and DPM2. The latter is not essential for activity.

    Protein-protein interaction databases

    BioGridi111301. 5 interactions.
    IntActiQ14442. 1 interaction.
    STRINGi9606.ENSP00000216452.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14442.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PIGH family.Curated

    Phylogenomic databases

    eggNOGiNOG266799.
    HOGENOMiHOG000059602.
    HOVERGENiHBG025632.
    InParanoidiQ14442.
    KOiK03858.
    OMAiTKRICRC.
    PhylomeDBiQ14442.
    TreeFamiTF324479.

    Family and domain databases

    InterProiIPR019328. GPI-GlcNAc_Trfase_PIG-H_dom.
    [Graphical view]
    PfamiPF10181. PIG-H. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q14442-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDERSFSDI CGGRLALQRR YYSPSCREFC LSCPRLSLRS LTAVTCTVWL    50
    AAYGLFTLCE NSMILSAAIF ITLLGLLGYL HFVKIDQETL LIIDSLGIQM 100
    TSSYASGKES TTFIEMGKVK DIVINEAIYM QKVIYYLCIL LKDPVEPHGI 150
    SQVVPVFQSA KPRLDCLIEV YRSCQEILAH QKATSTSP 188
    Length:188
    Mass (Da):21,081
    Last modified:November 1, 1996 - v1
    Checksum:iEE70E4156272AC8B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19783 mRNA. Translation: AAA03545.1.
    BT006804 mRNA. Translation: AAP35450.1.
    AK314108 mRNA. Translation: BAG36801.1.
    CH471061 Genomic DNA. Translation: EAW80942.1.
    BC004100 mRNA. Translation: AAH04100.1.
    BC071849 mRNA. Translation: AAH71849.1.
    CCDSiCCDS9784.1.
    PIRiA48024.
    RefSeqiNP_004560.1. NM_004569.3.
    UniGeneiHs.553497.

    Genome annotation databases

    EnsembliENST00000216452; ENSP00000216452; ENSG00000100564.
    GeneIDi5283.
    KEGGihsa:5283.
    UCSCiuc001xjr.1. human.

    Polymorphism databases

    DMDMi27151659.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Phosphatidylinositol N-acetylglucosaminyltransferase subunit H

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19783 mRNA. Translation: AAA03545.1 .
    BT006804 mRNA. Translation: AAP35450.1 .
    AK314108 mRNA. Translation: BAG36801.1 .
    CH471061 Genomic DNA. Translation: EAW80942.1 .
    BC004100 mRNA. Translation: AAH04100.1 .
    BC071849 mRNA. Translation: AAH71849.1 .
    CCDSi CCDS9784.1.
    PIRi A48024.
    RefSeqi NP_004560.1. NM_004569.3.
    UniGenei Hs.553497.

    3D structure databases

    ProteinModelPortali Q14442.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111301. 5 interactions.
    IntActi Q14442. 1 interaction.
    STRINGi 9606.ENSP00000216452.

    PTM databases

    PhosphoSitei Q14442.

    Polymorphism databases

    DMDMi 27151659.

    Proteomic databases

    MaxQBi Q14442.
    PaxDbi Q14442.
    PRIDEi Q14442.

    Protocols and materials databases

    DNASUi 5283.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000216452 ; ENSP00000216452 ; ENSG00000100564 .
    GeneIDi 5283.
    KEGGi hsa:5283.
    UCSCi uc001xjr.1. human.

    Organism-specific databases

    CTDi 5283.
    GeneCardsi GC14M068056.
    HGNCi HGNC:8964. PIGH.
    HPAi HPA031624.
    MIMi 600154. gene.
    neXtProti NX_Q14442.
    PharmGKBi PA33295.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG266799.
    HOGENOMi HOG000059602.
    HOVERGENi HBG025632.
    InParanoidi Q14442.
    KOi K03858.
    OMAi TKRICRC.
    PhylomeDBi Q14442.
    TreeFami TF324479.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    Reactomei REACT_952. Synthesis of glycosylphosphatidylinositol (GPI).

    Miscellaneous databases

    GeneWikii PIGH.
    GenomeRNAii 5283.
    NextBioi 20416.
    PROi Q14442.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14442.
    Bgeei Q14442.
    CleanExi HS_PIGH.
    Genevestigatori Q14442.

    Family and domain databases

    InterProi IPR019328. GPI-GlcNAc_Trfase_PIG-H_dom.
    [Graphical view ]
    Pfami PF10181. PIG-H. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone."
      Kamitani T., Chang H.M., Rollins C., Waneck G.L., Yeh E.T.
      J. Biol. Chem. 268:20733-20736(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Placenta.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood and Lung.
    6. "The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1."
      Watanabe R., Inoue N., Westfall B., Taron C.H., Orlean P., Takeda J., Kinoshita T.
      EMBO J. 17:877-885(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIGA; PIGC AND PIGQ.
    7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiPIGH_HUMAN
    AccessioniPrimary (citable) accession number: Q14442
    Secondary accession number(s): B2RAA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3