cGMP-inhibited 3',5'-cyclic phosphodiesterase A

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Reviewed, UniProtKB/Swiss-Prot Q14432 (PDE3A_HUMAN)

Last modified July 7, 2009. Version 89. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
      Short name=CGI-PDE A

Gene names

Name:

PDE3A

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP) By similarity.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate.
Enzyme regulation	Inhibited by cGMP.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

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Ontologies

Keywords
Cellular component	Membrane
Ligand	cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid metabolic process Traceable author statement. Source: ProtInc signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1141

1141

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

PRO_0000198799

Regions

Compositional bias

95 – 98

Poly-Ala

Compositional bias

99 – 102

Poly-Glu

Compositional bias

288 – 291

Poly-Arg

Compositional bias

440 – 445

Poly-Thr

Compositional bias

870 – 873

Poly-Ala

Compositional bias

1040 – 1045

Poly-Glu

Compositional bias

1121 – 1125

Poly-Glu

Amino acid modifications

Modified residue

312

Phosphoserine Ref.8 Ref.10

Modified residue

428

Phosphoserine Ref.10 Ref.7

Modified residue

440

Phosphothreonine Ref.9

Modified residue

495

Phosphoserine By similarity

Experimental info

Sequence conflict

D → N in AAB18673. Ref.3

Sequence conflict

S → C in AAB18673. Ref.3

Sequence conflict

110

G → A in AAB18673. Ref.3

Sequence conflict

354 – 371

HGLIT…PPNVC → TASLPTSWQTLLFHQTCA Ref.3

Sequence conflict

354 – 371

HGLIT…PPNVC → TASLPTSWQTLLFHQTCA Ref.4

Secondary structure

1141

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q14432-1 [UniParc].

Last modified May 10, 2004. Version 3.
Checksum: E69504130F39EFEA
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FASTA

1,141

124,979

        10         20         30         40         50         60 
MAVPGDAARV RDKPVHSGVS QAPTAGRDCH HRADPASPRD SGCRGCWGDL VLQPLRSSRK 

        70         80         90        100        110        120 
LSSALCAGSL SFLLALLVRL VRGEVGCDLE QCKEAAAAEE EEAAPGAEGG VFPGPRGGAP 

       130        140        150        160        170        180 
GGGARLSPWL QPSALLFSLL CAFFWMGLYL LRAGVRLPLA VALLAACCGG EALVQIGLGV 

       190        200        210        220        230        240 
GEDHLLSLPA AGVVLSCLAA ATWLVLRLRL GVLMIALTSA VRTVSLISLE RFKVAWRPYL 

       250        260        270        280        290        300 
AYLAGVLGIL LARYVEQILP QSAEAAPREH LGSQLIAGTK EDIPVFKRRR RSSSVVSAEM 

       310        320        330        340        350        360 
SGCSSKSHRR TSLPCIPREQ LMGHSEWDHK RGPRGSQSSG TSITVDIAVM GEAHGLITDL 

       370        380        390        400        410        420 
LADPSLPPNV CTSLRAVSNL LSTQLTFQAI HKPRVNPVTS LSENYTCSDS EESSEKDKLA 

       430        440        450        460        470        480 
IPKRLRRSLP PGLLRRVSST WTTTTSATGL PTLEPAPVRR DRSTSIKLQE APSSSPDSWN 

       490        500        510        520        530        540 
NPVMMTLTKS RSFTSSYAIS AANHVKAKKQ SRPGALAKIS PLSSPCSSPL QGTPASSLVS 

       550        560        570        580        590        600 
KISAVQFPES ADTTAKQSLG SHRALTYTQS APDLSPQILT PPVICSSCGR PYSQGNPADE 

       610        620        630        640        650        660 
PLERSGVATR TPSRTDDTAQ VTSDYETNNN SDSSDIVQNE DETECLREPL RKASACSTYA 

       670        680        690        700        710        720 
PETMMFLDKP ILAPEPLVMD NLDSIMEQLN TWNFPIFDLV ENIGRKCGRI LSQVSYRLFE 

       730        740        750        760        770        780 
DMGLFEAFKI PIREFMNYFH ALEIGYRDIP YHNRIHATDV LHAVWYLTTQ PIPGLSTVIN 

       790        800        810        820        830        840 
DHGSTSDSDS DSGFTHGHMG YVFSKTYNVT DDKYGCLSGN IPALELMALY VAAAMHDYDH 

       850        860        870        880        890        900 
PGRTNAFLVA TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLINLD HVEFKHFRFL 

       910        920        930        940        950        960 
VIEAILATDL KKHFDFVAKF NGKVNDDVGI DWTNENDRLL VCQMCIKLAD INGPAKCKEL 

       970        980        990       1000       1010       1020 
HLQWTDGIVN EFYEQGDEEA SLGLPISPFM DRSAPQLANL QESFISHIVG PLCNSYDSAG 

      1030       1040       1050       1060       1070       1080 
LMPGKWVEDS DESGDTDDPE EEEEEAPAPN EEETCENNES PKKKTFKRRK IYCQITQHLL 

      1090       1100       1110       1120       1130       1140 
QNHKMWKKVI EEEQRLAGIE NQSLDQTPQS HSSEQIQAIK EEEEEKGKPR GEEIPTQKPD 


Q

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase." Meacci E., Taira M., Moos M. Jr., Smith C.J., Movsesian M.A., Degerman E., Belfrage P., Manganiello V. Proc. Natl. Acad. Sci. U.S.A. 89:3721-3725(1992) [PubMed: 1315035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Myocardium.
[2]	Liu H., Manganiello V. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 12; 63-64 AND 354-371.
[3]	"Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis." Cheung P.P., Xu H., McLaughlin M.M., Ghazaleh F.A., Livi G.P., Colman R.W. Blood 88:1321-1329(1996) [PubMed: 8695850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
[4]	"Molecular biological characterization of phosphodiesterase 3A from human corpus cavernosum." Kuthe A., Eckel H., Stief C.G., Uckert S., Forssmann W.-G., Jonas U., Maegert H.-J. Chem. Biol. Interact. 119:593-598(1999) [PubMed: 10421499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Penis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[6]	"Single-step affinity purification, partial structure and properties of human platelet cGMP inhibited cAMP phosphodiesterase." Degerman E., Moos M. Jr., Rascon A., Vasta V., Meacci E., Smith C.J., Lindgren S., Andersson K.-E., Belfrage P., Manganiello V. Biochim. Biophys. Acta 1205:189-198(1994) [PubMed: 8155697] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312, MASS SPECTROMETRY. Tissue: Platelet.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-440, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND SER-428, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Web resources

Wikipedia

PDE3 entry

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Cross-references

Sequence databases

M91667 mRNA. Translation: AAA35912.2.
U36798 mRNA. Translation: AAB18673.1.
AJ005036 mRNA. Translation: CAA06304.1.
BC117369 mRNA. Translation: AAI17370.1.
BC117371 mRNA. Translation: AAI17372.1.

IPI

IPI00291205.

PIR

A44093.

RefSeq

NP_000912.3.

UniGene

Hs.591150

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LRC	model	-	A	812-1017	[»]

SMR

Q14432. Positions 677-1049.

ModBase

Search...

PTM databases

PhosphoSite

Q14432.

Proteomic databases

PRIDE

Q14432.

Genome annotation databases

Ensembl

ENSG00000172572. Homo sapiens. [Contig view]

GeneID

5139.

KEGG

hsa:5139.

UCSC

uc001reh.1. human.

Organism-specific databases

GeneCards

GC12P020413.

HGNC

HGNC:8778. PDE3A.

HPA

HPA014492.

MIM

123805. gene.

PharmGKB

PA33126.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q14432.

HOVERGEN

Q14432.

OMA

Q14432. DSGFTHG.

Enzyme and pathway databases

BRENDA

3.1.4.17. 247.

Gene expression databases

ArrayExpress

Q14432.

Bgee

Q14432.

CleanEx

HS_PDE3A.

GermOnline

ENSG00000172572. Homo sapiens.

Family and domain databases

InterPro

IPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]

Pfam

PF00233. PDEase_I. 1 hit.
[Graphical view]

SMART

SM00471. HDc. 1 hit.
[Graphical view]

PROSITE

PS00126. PDEASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB01223. Aminophylline.
DB01427. Amrinone.
DB00261. Anagrelide.
DB01166. Cilostazol.
DB04880. Enoximone.
DB00235. Milrinone.
DB00277. Theophylline.

NextBio

19816.

SOURCE

Search...

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Entry information

Entry name

PDE3A_HUMAN

Accession

Primary (citable) accession number: Q14432
Secondary accession number(s): O60865, Q13348, Q17RD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 10, 2004
Last modified:	July 7, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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