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Q14432

- PDE3A_HUMAN

UniProt

Q14432 - PDE3A_HUMAN

Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

PDE3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 3 (10 May 2004)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by cGMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei752 – 7521Proton donorBy similarity
    Metal bindingi756 – 7561Divalent metal cation 1By similarity
    Metal bindingi836 – 8361Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 2By similarity
    Metal bindingi950 – 9501Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: Ensembl
    2. cAMP binding Source: Ensembl
    3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: ProtInc
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. cAMP catabolic process Source: UniProtKB
    3. cAMP-mediated signaling Source: UniProtKB
    4. cellular response to cGMP Source: UniProtKB
    5. cellular response to transforming growth factor beta stimulus Source: UniProtKB
    6. cGMP-mediated signaling Source: UniProtKB
    7. diterpenoid metabolic process Source: Ensembl
    8. lipid metabolic process Source: ProtInc
    9. negative regulation of apoptotic process Source: Ensembl
    10. negative regulation of vascular permeability Source: UniProtKB
    11. oocyte maturation Source: Ensembl
    12. positive regulation of oocyte development Source: Ensembl
    13. positive regulation of vascular permeability Source: UniProtKB
    14. regulation of meiosis Source: Ensembl
    15. response to cAMP Source: Ensembl
    16. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_19327. G alpha (s) signalling events.
    REACT_23767. cGMP effects.
    SABIO-RKQ14432.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
    Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
    Short name:
    CGI-PDE A
    Gene namesi
    Name:PDE3A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:8778. PDE3A.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33126.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase APRO_0000198799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei312 – 3121Phosphoserine1 Publication
    Modified residuei520 – 5201Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ14432.
    PaxDbiQ14432.
    PRIDEiQ14432.

    PTM databases

    PhosphoSiteiQ14432.

    Expressioni

    Gene expression databases

    BgeeiQ14432.
    CleanExiHS_PDE3A.
    GenevestigatoriQ14432.

    Organism-specific databases

    HPAiHPA014492.

    Interactioni

    Protein-protein interaction databases

    BioGridi111165. 13 interactions.
    DIPiDIP-42197N.
    IntActiQ14432. 2 interactions.
    MINTiMINT-3295914.
    STRINGi9606.ENSP00000351957.

    Structurei

    Secondary structure

    1
    1141
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi815 – 8173
    Helixi823 – 83513
    Turni836 – 8394
    Helixi845 – 8506
    Helixi854 – 8585
    Turni859 – 8613
    Helixi864 – 87613
    Turni877 – 8793
    Turni885 – 8884
    Helixi891 – 90616
    Helixi910 – 9123
    Helixi913 – 9186
    Helixi939 – 95012
    Helixi953 – 9553
    Helixi958 – 98124
    Helixi997 – 100711
    Helixi1009 – 10168

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LRCmodel-A812-1017[»]
    ProteinModelPortaliQ14432.
    SMRiQ14432. Positions 677-1087.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei61 – 8121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei160 – 18021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei210 – 23021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni728 – 1086359CatalyticBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi95 – 984Poly-Ala
    Compositional biasi99 – 1024Poly-Glu
    Compositional biasi288 – 2914Poly-Arg
    Compositional biasi440 – 4456Poly-Thr
    Compositional biasi870 – 8734Poly-Ala
    Compositional biasi1040 – 10456Poly-Glu
    Compositional biasi1121 – 11255Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ14432.
    KOiK13296.
    OMAiPETMMFL.
    OrthoDBiEOG7KDF95.
    PhylomeDBiQ14432.
    TreeFamiTF329631.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q14432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPGDAARV RDKPVHSGVS QAPTAGRDCH HRADPASPRD SGCRGCWGDL     50
    VLQPLRSSRK LSSALCAGSL SFLLALLVRL VRGEVGCDLE QCKEAAAAEE 100
    EEAAPGAEGG VFPGPRGGAP GGGARLSPWL QPSALLFSLL CAFFWMGLYL 150
    LRAGVRLPLA VALLAACCGG EALVQIGLGV GEDHLLSLPA AGVVLSCLAA 200
    ATWLVLRLRL GVLMIALTSA VRTVSLISLE RFKVAWRPYL AYLAGVLGIL 250
    LARYVEQILP QSAEAAPREH LGSQLIAGTK EDIPVFKRRR RSSSVVSAEM 300
    SGCSSKSHRR TSLPCIPREQ LMGHSEWDHK RGPRGSQSSG TSITVDIAVM 350
    GEAHGLITDL LADPSLPPNV CTSLRAVSNL LSTQLTFQAI HKPRVNPVTS 400
    LSENYTCSDS EESSEKDKLA IPKRLRRSLP PGLLRRVSST WTTTTSATGL 450
    PTLEPAPVRR DRSTSIKLQE APSSSPDSWN NPVMMTLTKS RSFTSSYAIS 500
    AANHVKAKKQ SRPGALAKIS PLSSPCSSPL QGTPASSLVS KISAVQFPES 550
    ADTTAKQSLG SHRALTYTQS APDLSPQILT PPVICSSCGR PYSQGNPADE 600
    PLERSGVATR TPSRTDDTAQ VTSDYETNNN SDSSDIVQNE DETECLREPL 650
    RKASACSTYA PETMMFLDKP ILAPEPLVMD NLDSIMEQLN TWNFPIFDLV 700
    ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PIREFMNYFH ALEIGYRDIP 750
    YHNRIHATDV LHAVWYLTTQ PIPGLSTVIN DHGSTSDSDS DSGFTHGHMG 800
    YVFSKTYNVT DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA 850
    TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLINLD HVEFKHFRFL 900
    VIEAILATDL KKHFDFVAKF NGKVNDDVGI DWTNENDRLL VCQMCIKLAD 950
    INGPAKCKEL HLQWTDGIVN EFYEQGDEEA SLGLPISPFM DRSAPQLANL 1000
    QESFISHIVG PLCNSYDSAG LMPGKWVEDS DESGDTDDPE EEEEEAPAPN 1050
    EEETCENNES PKKKTFKRRK IYCQITQHLL QNHKMWKKVI EEEQRLAGIE 1100
    NQSLDQTPQS HSSEQIQAIK EEEEEKGKPR GEEIPTQKPD Q 1141
    Length:1,141
    Mass (Da):124,979
    Last modified:May 10, 2004 - v3
    Checksum:iE69504130F39EFEA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691S → C in AAB18673. (PubMed:8695850)Curated
    Sequence conflicti110 – 1101G → A in AAB18673. (PubMed:8695850)Curated
    Sequence conflicti354 – 37118HGLIT…PPNVC → TASLPTSWQTLLFHQTCA(PubMed:8695850)CuratedAdd
    BLAST
    Sequence conflicti354 – 37118HGLIT…PPNVC → TASLPTSWQTLLFHQTCA(PubMed:10421499)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121D → N.1 Publication
    Corresponds to variant rs12305038 [ dbSNP | Ensembl ].
    VAR_059543

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91667 mRNA. Translation: AAA35912.2.
    U36798 mRNA. Translation: AAB18673.1.
    AJ005036 mRNA. Translation: CAA06304.1.
    BC117369 mRNA. Translation: AAI17370.1.
    BC117371 mRNA. Translation: AAI17372.1.
    CCDSiCCDS31754.1.
    PIRiA44093.
    RefSeqiNP_000912.3. NM_000921.4.
    UniGeneiHs.386791.
    Hs.737522.

    Genome annotation databases

    EnsembliENST00000359062; ENSP00000351957; ENSG00000172572.
    GeneIDi5139.
    KEGGihsa:5139.
    UCSCiuc001reh.2. human.

    Polymorphism databases

    DMDMi47117888.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    PDE3 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91667 mRNA. Translation: AAA35912.2 .
    U36798 mRNA. Translation: AAB18673.1 .
    AJ005036 mRNA. Translation: CAA06304.1 .
    BC117369 mRNA. Translation: AAI17370.1 .
    BC117371 mRNA. Translation: AAI17372.1 .
    CCDSi CCDS31754.1.
    PIRi A44093.
    RefSeqi NP_000912.3. NM_000921.4.
    UniGenei Hs.386791.
    Hs.737522.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LRC model - A 812-1017 [» ]
    ProteinModelPortali Q14432.
    SMRi Q14432. Positions 677-1087.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111165. 13 interactions.
    DIPi DIP-42197N.
    IntActi Q14432. 2 interactions.
    MINTi MINT-3295914.
    STRINGi 9606.ENSP00000351957.

    Chemistry

    BindingDBi Q14432.
    ChEMBLi CHEMBL2095153.
    DrugBanki DB01223. Aminophylline.
    DB01427. Amrinone.
    DB00261. Anagrelide.
    DB01166. Cilostazol.
    DB04880. Enoximone.
    DB00235. Milrinone.
    DB00277. Theophylline.
    GuidetoPHARMACOLOGYi 1298.

    PTM databases

    PhosphoSitei Q14432.

    Polymorphism databases

    DMDMi 47117888.

    Proteomic databases

    MaxQBi Q14432.
    PaxDbi Q14432.
    PRIDEi Q14432.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359062 ; ENSP00000351957 ; ENSG00000172572 .
    GeneIDi 5139.
    KEGGi hsa:5139.
    UCSCi uc001reh.2. human.

    Organism-specific databases

    CTDi 5139.
    GeneCardsi GC12P020422.
    HGNCi HGNC:8778. PDE3A.
    HPAi HPA014492.
    MIMi 123805. gene.
    neXtProti NX_Q14432.
    PharmGKBi PA33126.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG145074.
    HOGENOMi HOG000060144.
    HOVERGENi HBG053541.
    InParanoidi Q14432.
    KOi K13296.
    OMAi PETMMFL.
    OrthoDBi EOG7KDF95.
    PhylomeDBi Q14432.
    TreeFami TF329631.

    Enzyme and pathway databases

    Reactomei REACT_19327. G alpha (s) signalling events.
    REACT_23767. cGMP effects.
    SABIO-RK Q14432.

    Miscellaneous databases

    ChiTaRSi PDE3A. human.
    GenomeRNAii 5139.
    NextBioi 19816.
    PROi Q14432.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q14432.
    CleanExi HS_PDE3A.
    Genevestigatori Q14432.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase."
      Meacci E., Taira M., Moos M. Jr., Smith C.J., Movsesian M.A., Degerman E., Belfrage P., Manganiello V.
      Proc. Natl. Acad. Sci. U.S.A. 89:3721-3725(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Myocardium.
    2. Liu H., Manganiello V.
      Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 12; 63-64 AND 354-371.
    3. "Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis."
      Cheung P.P., Xu H., McLaughlin M.M., Ghazaleh F.A., Livi G.P., Colman R.W.
      Blood 88:1321-1329(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-12.
      Tissue: Blood.
    4. "Molecular biological characterization of phosphodiesterase 3A from human corpus cavernosum."
      Kuthe A., Eckel H., Stief C.G., Uckert S., Forssmann W.-G., Jonas U., Maegert H.-J.
      Chem. Biol. Interact. 119:593-598(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Penis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    6. "Single-step affinity purification, partial structure and properties of human platelet cGMP inhibited cAMP phosphodiesterase."
      Degerman E., Moos M. Jr., Rascon A., Vasta V., Meacci E., Smith C.J., Lindgren S., Andersson K.-E., Belfrage P., Manganiello V.
      Biochim. Biophys. Acta 1205:189-198(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
    7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Platelet.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPDE3A_HUMAN
    AccessioniPrimary (citable) accession number: Q14432
    Secondary accession number(s): O60865, Q13348, Q17RD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 137 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3