Q14410 (GLPK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase 2
Short name=GK 2
Short name=Glycerokinase 2
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase 2
Glycerol kinase, testis specific 2

Gene names

Name:	GK2
Synonyms:	GKP2, GKTA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Subcellular location	Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: In sperm the majority of the enzyme is bound to mitochondria By similarity.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol metabolic process Non-traceable author statement. Source: UniProtKB glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial outer membrane Non-traceable author statement. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Glycerol kinase 2		PRO_0000059536
Regions
	Nucleotide binding	427 – 431	5	ATP By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	24	1	ATP By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	281	1	ATP By similarity
	Binding site	326	1	ATP; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	238	1	E → G in BAF84930. Ref.2
	Sequence conflict	369	1	A → T in BAF84930. Ref.2
	Sequence conflict	499	1	R → C in BAF84930. Ref.2
	Sequence conflict	528	1	S → C in CAA55365. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q14410 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 8CE43A0686BC4AD6	FASTA	553	60,594
	10 20 30 40 50 60 MAAPKTAAVG PLVGAVVQGT NSTRFLVFNS KTAELLSHHK VELTQEFPKE GWVEQDPKEI 70 80 90 100 110 120 LQSVYECIAR TCEKLDELNI DISNIKAVGV SNQRETTVIW DKLTGEPLYN AVVWLDLRTQ 130 140 150 160 170 180 TTVEDLSKKI PGNSNFVKSK TGLPLSTYFS AVKLRWMLDN VRNVQKAVEE GRALFGTIDS 190 200 210 220 230 240 WLIWSLTGGV NGGVHCTDVT NASRTMLFNI HSLEWDKELC DFFEIPMDLL PNVFSSSEIY 250 260 270 280 290 300 GLIKTGALEG VPISGCLGDQ CAALVGQMCF QEGQAKNTYG TGCFLLCNTG RKCVFSEHGL 310 320 330 340 350 360 LTTVAYKLGR EKPAYYALEG SVAIAGAVIR WLRDNLGIIE TSGDIERLAK EVGTSYGCYF 370 380 390 400 410 420 VPAFSGLYAP YWEPSARGIL CGLTQFTNKC HIAFAALEAV CFQTREILEA MNRDCGIPLR 430 440 450 460 470 480 HLQVDGGMTN NKVLMQLQAD ILHIPVIKPF MPETTALGAA MAAGAAEGVS VWSLEPQALS 490 500 510 520 530 540 VLRMERFEPQ IQATESEIRY ATWKKAVMKS MGWVTSQSPE GGDPSIFSSL PLGFFIVSSM 550 VMLIGARYIS GVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glycerol kinase gene family: structure of the Xp gene, and related intronless retroposons." Sargent C.A., Young C., Marsh S., Ferguson-Smith M.A., Affara N.A. Hum. Mol. Genet. 3:1317-1324(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78712 mRNA. Translation: CAA55365.1. AK292241 mRNA. Translation: BAF84930.1. CH471057 Genomic DNA. Translation: EAX05842.1. BC029820 mRNA. Translation: AAH29820.1. BC048274 mRNA. Translation: AAH48274.2. BC058888 mRNA. Translation: AAH58888.1.
IPI	IPI00419234.
PIR	I37417.
RefSeq	NP_149991.2. NM_033214.2.
UniGene	Hs.98008.
3D structure databases
ProteinModelPortal	Q14410.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000351706.
PTM databases
PhosphoSite	Q14410.
Polymorphism databases
DMDM	212286188.
Proteomic databases
PaxDb	Q14410.
PRIDE	Q14410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358842; ENSP00000351706; ENSG00000196475.
GeneID	2712.
KEGG	hsa:2712.
UCSC	uc003hlu.3. human.
Organism-specific databases
CTD	2712.
GeneCards	GC04M080255.
H-InvDB	HIX0004328.
HGNC	HGNC:4291. GK2.
MIM	600148. gene.
neXtProt	NX_Q14410.
PharmGKB	PA28702.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HOG000222134.
HOVERGEN	HBG002451.
InParanoid	Q14410.
KO	K00864.
OMA	GQKEFTQ.
OrthoDB	EOG41G33T.
Enzyme and pathway databases
UniPathway	UPA00618; UER00672.
Gene expression databases
Bgee	Q14410.
Genevestigator	Q14410.
GermOnline	ENSG00000196475. Homo sapiens.
Family and domain databases
InterPro	IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196:SF9. PTHR10196:SF9. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2712.
NextBio	10720.
SOURCE	Search...

Entry information

Entry name

GLPK2_HUMAN

Accession

Primary (citable) accession number: Q14410
Secondary accession number(s): A8K876, Q6PD73, Q86XV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.