Q14409 (GLPK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glycerol kinase 3
Short name=GK 3
Short name=Glycerokinase 3
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase 3
Glycerol kinase, testis specific 1

Gene names

Name:	GK3P
Synonyms:	GKP3, GKTB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Subcellular location	Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: In sperm the majority of the enzyme is bound to mitochondria By similarity.
Sequence similarities	Belongs to the FGGY kinase family.
Caution	Could be the product of a pseudogene.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial outer membrane Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Putative glycerol kinase 3		PRO_0000059537
Regions
	Nucleotide binding	427 – 431	5	ATP By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	24	1	ATP By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	281	1	ATP By similarity
	Binding site	326	1	ATP; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	99	1	V → A in CAA55364. Ref.1
	Sequence conflict	485	1	E → K in BC066960. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q14409 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 1D6A197F32A921FB	FASTA	553	60,598
	10 20 30 40 50 60 MAASKKAVLG PLVGAVDQGT SSTRFLVFNS RTAELLSHHQ VEIKQEFPRE GWVEQDPKEI 70 80 90 100 110 120 LHSVYECIEK TCEKLGQLNI GISNIKAIGV SNQRETTVVW DKITGEPLYN AVVWLDLRTQ 130 140 150 160 170 180 STVESLSKRI PGNNNFVKSK TGLPLSTYFS AVKLRWLLDN VRKVQKAVEE KRALFGTIDS 190 200 210 220 230 240 WLIWSLTGGV NGGVHCTDVT NASRTMLFNI HSLEWDKQLC EFFGIPMEIL PHVRSSSEIY 250 260 270 280 290 300 GLMKAGALEG VPISGCLGDQ SAALVGQMCF QIGQAKNTYG TGCFLLCNTG HKCVFSDHGL 310 320 330 340 350 360 LTTVAYKLGR DKPVYYALEG SVAIAGAVIR WLRDNLGIIK TSEEIEKLAK EVGTSYGCYF 370 380 390 400 410 420 VPAFSGLYAP YWEPSARGII CGLTQFTNKC HIAFAALEAV CFQTREILDA MNRDCGIPLS 430 440 450 460 470 480 HLQVDGGMTS NKILMQLQAD ILYIPVVKPL MPETTALGAA MAAGAAEGVD VWSLEPEDLS 490 500 510 520 530 540 AVTMERFEPQ INAEESEIRY STWKKAVMKS MGWVTTQSPE GGDPSVFCSL PLGFFIVSSM 550 AMLIGARYIS GIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glycerol kinase gene family: structure of the Xp gene, and related intronless retroposons." Sargent C.A., Young C., Marsh S., Ferguson-Smith M.A., Affara N.A. Hum. Mol. Genet. 3:1317-1324(1994) [PubMed: 7987308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78711 mRNA. Translation: CAA55364.1. AC107059 Genomic DNA. No translation available. BC066960 mRNA. No translation available.
IPI	IPI00030811.
3D structure databases
ProteinModelPortal	Q14409.
SMR	Q14409. Positions 11-516.
ModBase	Search...
Protein-protein interaction databases
STRING	Q14409.
Polymorphism databases
DMDM	215277039.
Proteomic databases
PRIDE	Q14409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
GeneCards	GC04M166198.
H-InvDB	HIX0031473.
HGNC	HGNC:4292. GK3P.
MIM	600149. gene.
neXtProt	NX_Q14409.
PharmGKB	PA28703.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07530.
HOGENOM	HBG511469.
HOVERGEN	HBG002451.
OMA	ALYGQLC.
PhylomeDB	Q14409.
Gene expression databases
Genevestigator	Q14409.
GermOnline	ENSG00000170095. Homo sapiens.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

GLPK3_HUMAN

Accession

Primary (citable) accession number: Q14409
Secondary accession number(s): Q6NXP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.