Reviewed, UniProtKB/Swiss-Prot Q14397 (GCKR_HUMAN)

Names and origin

Protein names

Recommended name:
    Glucokinase regulatory protein
      Short name=Glucokinase regulator

Gene names

Name:

GCKR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits glucokinase by forming an inactive complex with this enzyme.
Tissue specificity	Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Sequence similarities	Belongs to the GCKR family. Contains 1 SIS domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Gene Ontology (GO)
Cellular component	cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	enzyme inhibitor activity Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCK	P35557	2	EBI-709948,EBI-709928

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 625	624	Glucokinase regulatory protein		PRO_0000214826
Regions
	Domain	93 – 259	167	SIS
Natural variations
	Natural variant	77	1	E → G: dbSNP rs8179206.		VAR_018849
	Natural variant	256	1	G → S: dbSNP rs8179212.		VAR_018850
	Natural variant	446	1	P → L: dbSNP rs1260326.		VAR_008906
	Natural variant	540	1	R → Q: dbSNP rs8179249.		VAR_018851

Sequences

	Sequence		Length	Mass (Da)
	Q14397-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: DE750462AC603C80	FASTA	625	68,685
	10 20 30 40 50 60 MPGTKRFQHV IETPEPGKWE LSGYEAAVPI TEKSNPLTQD LDKADAENIV RLLGQCDAEI 70 80 90 100 110 120 FQEEGQALST YQRLYSESIL TTMVQVAGKV QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS 130 140 150 160 170 180 FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE GTEDSALHGI EELKKVAAGK KRVIVIGISV 190 200 210 220 230 240 GLSAPFVAGQ MDCCMNNTAV FLPVLVGFNP VSMARNDPIE DWSSTFRQVA ERMQKMQEKQ 250 260 270 280 290 300 KAFVLNPAIG PEGLSGSSRM KGGSATKILL ETLLLAAHKT VDQGIAASQR CLLEILRTFE 310 320 330 340 350 360 RAHQVTYSQS PKIATLMKSV STSLEKKGHV YLVGWQTLGI IAIMDGVECI HTFGADFRDV 370 380 390 400 410 420 RGFLIGDHSD MFNQKAELTN QGPQFTFSQE DFLTSILPSL TEIDTVVFIF TLDDNLTEVQ 430 440 450 460 470 480 TIVEQVKEKT NHIQALAHST VGQTLPIPLK KLFPSIISIT WPLLFFEYEG NFIQKFQREL 490 500 510 520 530 540 STKWVLNTVS TGAHVLLGKI LQNHMLDLRI SNSKLFWRAL AMLQRFSGQS KARCIESLLR 550 560 570 580 590 600 AIHFPQPLSD DIRAAPISCH VQVAHEKEQV IPIALLSLLF RCSITEAQAH LAAAPSVCEA 610 620 VRSALAGPGQ KRTADPLEIL EPDVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human glucokinase regulatory protein (GCKR): cDNA and genomic cloning, complete primary structure, and chromosomal localization." Warner J.P., Leek J.P., Intody S., Markham A.F., Bonthron D.T. Mamm. Genome 6:532-536(1995) [PubMed: 8589523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Hepatoblastoma.
[2]	"Organization of the human glucokinase regulator gene GCKR." Hayward B.E., Dunlop N., Intody S., Leek J.P., Markham A.F., Warner J.P., Bonthron D.T. Genomics 49:137-142(1998) [PubMed: 9570959] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION TO 393 AND 560, VARIANT LEU-446, TISSUE SPECIFICITY.
[3]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Rieder M.J., Livingston R.J., Daniels M.R., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-77; SER-256; LEU-446 AND GLN-540.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-446.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48475 mRNA. Translation: CAA88367.1. Y09593 Genomic DNA. Translation: CAA70779.2. Y09592 Genomic DNA. Translation: CAB61828.1. AY320034 Genomic DNA. Translation: AAP72013.1. AC074117 Genomic DNA. Translation: AAY14850.1.
PIR	S52485.
RefSeq	NP_001477.1.
UniGene	Hs.89771
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14397.
PTM databases
PhosphoSite	Q14397.
Polymorphism databases
NIEHS-SNPs	Search...
Genome annotation databases
Ensembl	ENSG00000084734. Homo sapiens. [Contig view]
GeneID	2646.
KEGG	hsa:2646.
Organism-specific databases
H-InvDB	HIX0030026.
HGNC	HGNC:4196. GCKR.
MIM	600842. gene.
PharmGKB	PA28611.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q14397.
HOVERGEN	Q14397.
Enzyme and pathway databases
Reactome	REACT_1709. Metabolism of small molecules.
Gene expression databases
ArrayExpress	Q14397.
CleanEx	HS_GCKR.
GermOnline	ENSG00000084734. Homo sapiens.
Family and domain databases
InterPro	IPR005486. Glucokinase_regulatory_CS. [Graphical view]
ProDom	PD021526. GCKR_vertebrate. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01272. GCKR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	10442.
SOURCE	Search...

Entry information

Entry name

GCKR_HUMAN

Accession

Primary (citable) accession number: Q14397
Secondary accession number(s): Q53RY6, Q99522

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 59 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)