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Protein

Guanine nucleotide-binding protein subunit alpha-13

Gene

GNA13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62MagnesiumBy similarity1
Metal bindingi203MagnesiumBy similarity1
Binding sitei349GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 62GTPBy similarity8
Nucleotide bindingi197 – 203GTPBy similarity7
Nucleotide bindingi222 – 226GTPBy similarity5
Nucleotide bindingi291 – 294GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120063-MONOMER.
ReactomeiR-HSA-193648. NRAGE signals death through JNK.
R-HSA-194840. Rho GTPase cycle.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
SignaLinkiQ14344.
SIGNORiQ14344.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-13
Short name:
G alpha-13
Short name:
G-protein subunit alpha-13
Gene namesi
Name:GNA13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:4381. GNA13.

Subcellular locationi

  • Membrane; Lipid-anchor
  • Melanosome
  • Cytoplasm
  • Nucleus

  • Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages. In round spermatids, also present in the nuclei.

GO - Cellular componenti

  • brush border membrane Source: GO_Central
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • heterotrimeric G-protein complex Source: GO_Central
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication1
Mutagenesisi18C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication1
Mutagenesisi203T → A: Abolishes phosphorylation by PKA; disrupts heterotrimer stability. 1 Publication1

Organism-specific databases

DisGeNETi10672.
OpenTargetsiENSG00000120063.
PharmGKBiPA28766.

Polymorphism and mutation databases

DMDMi38258936.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002037731 – 377Guanine nucleotide-binding protein subunit alpha-13Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi14S-palmitoyl cysteine1 Publication1
Lipidationi18S-palmitoyl cysteine1 Publication1
Modified residuei203Phosphothreonine; by PKA1 Publication1

Post-translational modificationi

Palmitoylation is critical for proper membrane localization and signaling.1 Publication
Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ14344.
MaxQBiQ14344.
PaxDbiQ14344.
PeptideAtlasiQ14344.
PRIDEiQ14344.

PTM databases

iPTMnetiQ14344.
PhosphoSitePlusiQ14344.
SwissPalmiQ14344.

Expressioni

Tissue specificityi

Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000120063.
CleanExiHS_GNA13.
ExpressionAtlasiQ14344. baseline and differential.
GenevisibleiQ14344. HS.

Organism-specific databases

HPAiHPA010087.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts with HAX1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF1Q928883EBI-465387,EBI-465400
CXCR5P323029EBI-465387,EBI-2835269

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115914. 54 interactors.
IntActiQ14344. 9 interactors.
MINTiMINT-85993.
STRINGi9606.ENSP00000400717.

Structurei

3D structure databases

ProteinModelPortaliQ14344.
SMRiQ14344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(12) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ14344.
KOiK04639.
OMAiQMFMSVR.
OrthoDBiEOG091G0NV2.
PhylomeDBiQ14344.
TreeFamiTF300673.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000469. Gprotein_alpha_12/13.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14344-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADFLPSRSV LSVCFPGCLL TSGEAEQQRK SKEIDKCLSR EKTYVKRLVK
60 70 80 90 100
ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV
110 120 130 140 150
DAREKLHIPW GDNSNQQHGD KMMSFDTRAP MAAQGMVETR VFLQYLPAIR
160 170 180 190 200
ALWADSGIQN AYDRRREFQL GESVKYFLDN LDKLGEPDYI PSQQDILLAR
210 220 230 240 250
RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS
260 270 280 290 300
EFDQVLMEDR LTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV
310 320 330 340 350
QIVSIKDYFL EFEGDPHCLR DVQKFLVECF RNKRRDQQQK PLYHHFTTAI
360 370
NTENIRLVFR DVKDTILHDN LKQLMLQ
Length:377
Mass (Da):44,050
Last modified:October 31, 2003 - v2
Checksum:i929B7B6473C54F2E
GO
Isoform 2 (identifier: Q14344-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.

Show »
Length:282
Mass (Da):33,287
Checksum:iE32B6298504794BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211F → L in BAH13696 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017160221V → L.1 PublicationCorresponds to variant rs1062597dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0551401 – 95Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22075 mRNA. Translation: AAA74235.1.
AF493902 mRNA. Translation: AAM12616.1.
AK302400 mRNA. Translation: BAH13696.1.
AK313672 mRNA. Translation: BAG36424.1.
AC037487 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW88993.1.
BC036756 mRNA. Translation: AAH36756.1.
CCDSiCCDS11661.1. [Q14344-1]
CCDS62302.1. [Q14344-2]
PIRiI57490.
RefSeqiNP_001269354.1. NM_001282425.1. [Q14344-2]
NP_006563.2. NM_006572.5. [Q14344-1]
UniGeneiHs.515018.

Genome annotation databases

EnsembliENST00000439174; ENSP00000400717; ENSG00000120063. [Q14344-1]
ENST00000541118; ENSP00000439647; ENSG00000120063. [Q14344-2]
GeneIDi10672.
KEGGihsa:10672.
UCSCiuc002jfc.4. human. [Q14344-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22075 mRNA. Translation: AAA74235.1.
AF493902 mRNA. Translation: AAM12616.1.
AK302400 mRNA. Translation: BAH13696.1.
AK313672 mRNA. Translation: BAG36424.1.
AC037487 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW88993.1.
BC036756 mRNA. Translation: AAH36756.1.
CCDSiCCDS11661.1. [Q14344-1]
CCDS62302.1. [Q14344-2]
PIRiI57490.
RefSeqiNP_001269354.1. NM_001282425.1. [Q14344-2]
NP_006563.2. NM_006572.5. [Q14344-1]
UniGeneiHs.515018.

3D structure databases

ProteinModelPortaliQ14344.
SMRiQ14344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115914. 54 interactors.
IntActiQ14344. 9 interactors.
MINTiMINT-85993.
STRINGi9606.ENSP00000400717.

PTM databases

iPTMnetiQ14344.
PhosphoSitePlusiQ14344.
SwissPalmiQ14344.

Polymorphism and mutation databases

DMDMi38258936.

Proteomic databases

EPDiQ14344.
MaxQBiQ14344.
PaxDbiQ14344.
PeptideAtlasiQ14344.
PRIDEiQ14344.

Protocols and materials databases

DNASUi10672.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000439174; ENSP00000400717; ENSG00000120063. [Q14344-1]
ENST00000541118; ENSP00000439647; ENSG00000120063. [Q14344-2]
GeneIDi10672.
KEGGihsa:10672.
UCSCiuc002jfc.4. human. [Q14344-1]

Organism-specific databases

CTDi10672.
DisGeNETi10672.
GeneCardsiGNA13.
HGNCiHGNC:4381. GNA13.
HPAiHPA010087.
MIMi604406. gene.
neXtProtiNX_Q14344.
OpenTargetsiENSG00000120063.
PharmGKBiPA28766.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ14344.
KOiK04639.
OMAiQMFMSVR.
OrthoDBiEOG091G0NV2.
PhylomeDBiQ14344.
TreeFamiTF300673.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120063-MONOMER.
ReactomeiR-HSA-193648. NRAGE signals death through JNK.
R-HSA-194840. Rho GTPase cycle.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
SignaLinkiQ14344.
SIGNORiQ14344.

Miscellaneous databases

ChiTaRSiGNA13. human.
GeneWikiiGNA13.
GenomeRNAii10672.
PROiQ14344.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120063.
CleanExiHS_GNA13.
ExpressionAtlasiQ14344. baseline and differential.
GenevisibleiQ14344. HS.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000469. Gprotein_alpha_12/13.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNA13_HUMAN
AccessioniPrimary (citable) accession number: Q14344
Secondary accession number(s): B2R977
, B7Z7R0, F5H1G8, Q8TD70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 31, 2003
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.