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Q14330 (GPR18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-arachidonyl glycine receptor

Short name=NAGly receptor
Alternative name(s):
G-protein coupled receptor 18
Gene names
Name:GPR18
Synonyms:GPCRW
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells. Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence AAG44671.1 differs from that shown. Reason: Frameshift at positions 78, 81 and 129.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331N-arachidonyl glycine receptor
PRO_0000069537

Regions

Topological domain1 – 2626Extracellular Potential
Transmembrane27 – 4721Helical; Name=1; Potential
Topological domain48 – 569Cytoplasmic Potential
Transmembrane57 – 7721Helical; Name=2; Potential
Topological domain78 – 9518Extracellular Potential
Transmembrane96 – 11621Helical; Name=3; Potential
Topological domain117 – 13822Cytoplasmic Potential
Transmembrane139 – 15921Helical; Name=4; Potential
Topological domain160 – 19132Extracellular Potential
Transmembrane192 – 21221Helical; Name=5; Potential
Topological domain213 – 23220Cytoplasmic Potential
Transmembrane233 – 25321Helical; Name=6; Potential
Topological domain254 – 26815Extracellular Potential
Transmembrane269 – 28921Helical; Name=7; Potential
Topological domain290 – 33142Cytoplasmic Potential

Amino acid modifications

Glycosylation141N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 172 By similarity

Experimental info

Sequence conflict121P → T in AAB65819. Ref.1
Sequence conflict861E → A in AAB65819. Ref.1
Sequence conflict971L → I in AAB65819. Ref.1
Sequence conflict1021V → G in AAG44671. Ref.3
Sequence conflict1121L → F in AAG44671. Ref.3
Sequence conflict1451V → SS in AAG44671. Ref.3
Sequence conflict3101M → L in AAB65819. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q14330 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 5B0B755A74FBBB36

FASTA33138,134
        10         20         30         40         50         60 
MITLNNQDQP VPFNSSHPDE YKIAALVFYS CIFIIGLFVN ITALWVFSCT TKKRTTVTIY 

        70         80         90        100        110        120 
MMNVALVDLI FIMTLPFRMF YYAKDEWPFG EYFCQILGAL TVFYPSIALW LLAFISADRY 

       130        140        150        160        170        180 
MAIVQPKYAK ELKNTCKAVL ACVGVWIMTL TTTTPLLLLY KDPDKDSTPA TCLKISDIIY 

       190        200        210        220        230        240 
LKAVNVLNLT RLTFFFLIPL FIMIGCYLVI IHNLLHGRTS KLKPKVKEKS IRIIITLLVQ 

       250        260        270        280        290        300 
VLVCFMPFHI CFAFLMLGTG ENSYNPWGAF TTFLMNLSTC LDVILYYIVS KQFQARVISV 

       310        320        330 
MLYRNYLRSM RRKSFRSGSL RSLSNINSEM L 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis."
Gantz I., Muraoka A., Yang Y.-K., Samuelson L.C., Zimmerman E.M., Cook H., Yamada T.
Genomics 42:462-466(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18."
Kohno M., Hasegawa H., Inoue A., Muraoka M., Miyazaki T., Oka K., Yasukawa M.
Biochem. Biophys. Res. Commun. 347:827-832(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]"Novel genes expressed in human dendritic cell."
Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Dendritic cell.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph and Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42324 Genomic DNA. Translation: AAB65819.1.
AF261135 mRNA. Translation: AAG44671.1. Frameshift.
BT009907 mRNA. Translation: AAP88909.1.
AL160155 Genomic DNA. Translation: CAI14306.1.
BC008569 mRNA. Translation: AAH08569.1.
BC050646 mRNA. Translation: AAH50646.1.
BC066927 mRNA. Translation: AAH66927.1.
RefSeqNP_001091670.1. NM_001098200.1.
NP_005283.1. NM_005292.3.
UniGeneHs.741589.

3D structure databases

ProteinModelPortalQ14330.
SMRQ14330. Positions 18-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000343428.

Chemistry

DrugBankDB00145. Glycine.
GuidetoPHARMACOLOGY89.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ14330.

Polymorphism databases

DMDM92087006.

Proteomic databases

PaxDbQ14330.
PRIDEQ14330.

Protocols and materials databases

DNASU2841.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000340807; ENSP00000343428; ENSG00000125245.
ENST00000397470; ENSP00000380610; ENSG00000125245.
ENST00000397473; ENSP00000380613; ENSG00000125245.
GeneID2841.
KEGGhsa:2841.
UCSCuc001voe.4. human.

Organism-specific databases

CTD2841.
GeneCardsGC13M099906.
HGNCHGNC:4472. GPR18.
HPAHPA013873.
MIM602042. gene.
neXtProtNX_Q14330.
PharmGKBPA28860.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG116115.
HOGENOMHOG000112785.
HOVERGENHBG105013.
InParanoidQ14330.
KOK08410.
OMAYAKGEWP.
OrthoDBEOG7SBNP4.
PhylomeDBQ14330.
TreeFamTF330775.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ14330.
BgeeQ14330.
CleanExHS_GPR18.
GenevestigatorQ14330.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028335. GPR18.
[Graphical view]
PANTHERPTHR24232:SF1. PTHR24232:SF1. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR18.
GenomeRNAi2841.
NextBio11205.
PROQ14330.
SOURCESearch...

Entry information

Entry nameGPR18_HUMAN
AccessionPrimary (citable) accession number: Q14330
Secondary accession number(s): Q6GTM3, Q96HI6, Q9H2L2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries