Q14330 (GPR18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-arachidonyl glycine receptor
Short name=NAGly receptor

Alternative name(s):
G-protein coupled receptor 18

Gene names

Name:	GPR18
Synonyms:	GPCRW

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells. Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAG44671.1 differs from that shown. Reason: Frameshift at positions 78, 81 and 129.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular function	G-protein coupled purinergic nucleotide receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	N-arachidonyl glycine receptor		PRO_0000069537
Regions
	Topological domain	1 – 26	26	Extracellular Potential
	Transmembrane	27 – 47	21	Helical; Name=1; Potential
	Topological domain	48 – 56	9	Cytoplasmic Potential
	Transmembrane	57 – 77	21	Helical; Name=2; Potential
	Topological domain	78 – 95	18	Extracellular Potential
	Transmembrane	96 – 116	21	Helical; Name=3; Potential
	Topological domain	117 – 138	22	Cytoplasmic Potential
	Transmembrane	139 – 159	21	Helical; Name=4; Potential
	Topological domain	160 – 191	32	Extracellular Potential
	Transmembrane	192 – 212	21	Helical; Name=5; Potential
	Topological domain	213 – 232	20	Cytoplasmic Potential
	Transmembrane	233 – 253	21	Helical; Name=6; Potential
	Topological domain	254 – 268	15	Extracellular Potential
	Transmembrane	269 – 289	21	Helical; Name=7; Potential
	Topological domain	290 – 331	42	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	14	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 172		By similarity
Experimental info
	Sequence conflict	12	1	P → T in AAB65819. Ref.1
	Sequence conflict	86	1	E → A in AAB65819. Ref.1
	Sequence conflict	97	1	L → I in AAB65819. Ref.1
	Sequence conflict	102	1	V → G in AAG44671. Ref.3
	Sequence conflict	112	1	L → F in AAG44671. Ref.3
	Sequence conflict	145	1	V → SS in AAG44671. Ref.3
	Sequence conflict	310	1	M → L in AAB65819. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q14330 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 5B0B755A74FBBB36	FASTA	331	38,134
	10 20 30 40 50 60 MITLNNQDQP VPFNSSHPDE YKIAALVFYS CIFIIGLFVN ITALWVFSCT TKKRTTVTIY 70 80 90 100 110 120 MMNVALVDLI FIMTLPFRMF YYAKDEWPFG EYFCQILGAL TVFYPSIALW LLAFISADRY 130 140 150 160 170 180 MAIVQPKYAK ELKNTCKAVL ACVGVWIMTL TTTTPLLLLY KDPDKDSTPA TCLKISDIIY 190 200 210 220 230 240 LKAVNVLNLT RLTFFFLIPL FIMIGCYLVI IHNLLHGRTS KLKPKVKEKS IRIIITLLVQ 250 260 270 280 290 300 VLVCFMPFHI CFAFLMLGTG ENSYNPWGAF TTFLMNLSTC LDVILYYIVS KQFQARVISV 310 320 330 MLYRNYLRSM RRKSFRSGSL RSLSNINSEM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis." Gantz I., Muraoka A., Yang Y.-K., Samuelson L.C., Zimmerman E.M., Cook H., Yamada T. Genomics 42:462-466(1997) [PubMed: 9205118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18." Kohno M., Hasegawa H., Inoue A., Muraoka M., Miyazaki T., Oka K., Yasukawa M. Biochem. Biophys. Res. Commun. 347:827-832(2006) [PubMed: 16844083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"Novel genes expressed in human dendritic cell." Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Dendritic cell.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph and Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42324 Genomic DNA. Translation: AAB65819.1. AF261135 mRNA. Translation: AAG44671.1. Frameshift. BT009907 mRNA. Translation: AAP88909.1. AL160155 Genomic DNA. Translation: CAI14306.1. BC008569 mRNA. Translation: AAH08569.1. BC050646 mRNA. Translation: AAH50646.1. BC066927 mRNA. Translation: AAH66927.1.
IPI	IPI00555618.
RefSeq	NP_001091670.1. NM_001098200.1. NP_005283.1. NM_005292.3.
UniGene	Hs.631765.
3D structure databases
ProteinModelPortal	Q14330.
SMR	Q14330. Positions 106-137, 270-304.
ModBase	Search...
Protein-protein interaction databases
STRING	Q14330.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q14330.
Polymorphism databases
DMDM	92087006.
Proteomic databases
PRIDE	Q14330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000340807; ENSP00000343428; ENSG00000125245. ENST00000397470; ENSP00000380610; ENSG00000125245. ENST00000397473; ENSP00000380613; ENSG00000125245.
GeneID	2841.
KEGG	hsa:2841.
UCSC	uc001voe.2. human.
Organism-specific databases
CTD	2841.
GeneCards	GC13M099906.
H-InvDB	HIX0011424.
HGNC	HGNC:4472. GPR18.
HPA	HPA013873.
MIM	602042. gene.
neXtProt	NX_Q14330.
PharmGKB	PA28860.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04852.
HOGENOM	HBG714987.
HOVERGEN	HBG105013.
InParanoid	Q14330.
OMA	LFVNVTA.
OrthoDB	EOG47H5QJ.
PhylomeDB	Q14330.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q14330.
Bgee	Q14330.
CleanEx	HS_GPR18.
Genevestigator	Q14330.
GermOnline	ENSG00000125245. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR002286. P2_purnocptor. [Graphical view]
KO	K08410.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01157. P2YPURNOCPTR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00145. Glycine.
NextBio	11205.
SOURCE	Search...

Entry information

Entry name

GPR18_HUMAN

Accession

Primary (citable) accession number: Q14330
Secondary accession number(s): Q6GTM3, Q96HI6, Q9H2L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.