Q14324 (MYPC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin-binding protein C, fast-type
Short name=Fast MyBP-C

Alternative name(s):
C-protein, skeletal muscle fast isoform

Gene names

Name:	MYBPC2
Synonyms:	MYBPCF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Sequence similarities	Belongs to the immunoglobulin superfamily. MyBP family. Contains 3 fibronectin type-III domains. Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Thick filament
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat
Ligand	Actin-binding
Molecular function	Muscle protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW muscle filament sliding Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome myosin filament Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of muscle Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYBPC1	Q00872	3	EBI-5653200,EBI-5652924
TTN	Q8WZ42	14	EBI-5653200,EBI-681210

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1141

1141

Myosin-binding protein C, fast-type

PRO_0000072691

Regions

Domain

50 – 153

104

Ig-like C2-type 1

Domain

255 – 344

90

Ig-like C2-type 2

Domain

345 – 437

93

Ig-like C2-type 3

Domain

438 – 538

101

Ig-like C2-type 4

Domain

539 – 638

100

Ig-like C2-type 5

Domain

639 – 731

93

Fibronectin type-III 1

Domain

736 – 829

94

Fibronectin type-III 2

Domain

838 – 932

95

Ig-like C2-type 6

Domain

932 – 1025

94

Fibronectin type-III 3

Domain

1048 – 1141

94

Ig-like C2-type 7

Natural variations

Natural variant

29

1

E → K.
Corresponds to variant rs57092106 [ dbSNP | Ensembl ].

VAR_061321

Natural variant

52

1

G → S.
Corresponds to variant rs25669 [ dbSNP | Ensembl ].

VAR_014657

Natural variant

282

1

D → N.
Corresponds to variant rs35951152 [ dbSNP | Ensembl ].

VAR_056060

Natural variant

341

1

V → I.
Corresponds to variant rs58511181 [ dbSNP | Ensembl ].

VAR_061322

Natural variant

514

1

G → S.
Corresponds to variant rs8104931 [ dbSNP | Ensembl ].

VAR_056061

Natural variant

624

1

V → I.
Corresponds to variant rs25665 [ dbSNP | Ensembl ].

VAR_014658

Natural variant

1089

1

R → H.
Corresponds to variant rs25667 [ dbSNP | Ensembl ].

VAR_014659

Experimental info

Sequence conflict

517

1

L → LG in CAA51544. Ref.1

Sequence conflict

820

1

S → T in CAA51544. Ref.1

Secondary structure

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1141

Helix Strand Turn

Details...

Beta strand

51 – 53

3

Beta strand

62 – 64

3

Beta strand

73 – 76

4

Turn

77 – 79

3

Beta strand

80 – 82

3

Beta strand

87 – 90

4

Turn

91 – 93

3

Beta strand

105 – 110

6

Beta strand

112 – 114

3

Beta strand

116 – 125

10

Turn

128 – 130

3

Beta strand

134 – 139

6

Beta strand

144 – 149

6

Beta strand

152 – 154

3

Beta strand

348 – 350

3

Beta strand

355 – 360

6

Beta strand

367 – 370

4

Beta strand

377 – 382

6

Beta strand

396 – 399

4

Beta strand

401 – 407

7

Helix

413 – 415

3

Beta strand

417 – 422

6

Beta strand

431 – 435

5

Beta strand

835 – 840

6

Turn

844 – 846

3

Beta strand

850 – 856

7

Beta strand

858 – 868

11

Beta strand

871 – 876

6

Beta strand

887 – 890

4

Beta strand

892 – 901

10

Turn

904 – 906

3

Beta strand

908 – 915

8

Beta strand

924 – 930

7

Sequences

	Sequence		Length	Mass (Da)
	Q14324 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 91F4B18C4367743A	FASTA	1,141	128,072
	10 20 30 40 50 60 MPEAKPAAKK APKGKDAPKG APKEAPPKEA PAEAPKEAPP EDQSPTAEEP TGVFLKKPDS 70 80 90 100 110 120 VSVETGKDAV VVAKVNGKEL PDKPTIKWFK GKWLELGSKS GARFSFKESH NSASNVYTVE 130 140 150 160 170 180 LHIGKVVLGD RGYYRLEVKA KDTCDSCGFN IDVEAPRQDA SGQSLESFKR TSEKKSDTAG 190 200 210 220 230 240 ELDFSGLLKK REVVEEEKKK KKKDDDDLGI PPEIWELLKG AKKSEYEKIA FQYGITDLRG 250 260 270 280 290 300 MLKRLKKAKV EVKKSAAFTK KLDPAYQVDR GNKIKLMVEI SDPDLTLKWF KNGQEIKPSS 310 320 330 340 350 360 KYVFENVGKK RILTINKCTL ADDAAYEVAV KDEKCFTELF VKEPPVLIVT PLEDQQVFVG 370 380 390 400 410 420 DRVEMAVEVS EEGAQVMWMK DGVELTREDS FKARYRFKKD GKRHILIFSD VVQEDRGRYQ 430 440 450 460 470 480 VITNGGQCEA ELIVEEKQLE VLQDIADLTV KASEQAVFKC EVSDEKVTGK WYKNGVEVRP 490 500 510 520 530 540 SKRITISHVG RFHKLVIDDV RPEDEGDYTF VPDGYALSLS AKLNFLEIKV EYVPKQEPPK 550 560 570 580 590 600 IHLDCSGKTS ENAIVVVAGN KLRLDVSITG EPPPVATWLK GDEVFTTTEG RTRIEKRVDC 610 620 630 640 650 660 SSFVIESAQR EDEGRYTIKV TNPVGEDVAS IFLQVVDVPD PPEAVRITSV GEDWAILVWE 670 680 690 700 710 720 PPMYDGGKPV TGYLVERKKK GSQRWMKLNF EVFTETTYES TKMIEGILYE MRVFAVNAIG 730 740 750 760 770 780 VSQPSMNTKP FMPIAPTSEP LHLIVEDVTD TTTTLKWRPP NRIGAGGIDG YLVEYCLEGS 790 800 810 820 830 840 EEWVPANTEP VERCGFTVKN LPTGARILFR VVGVNIAGRS EPATLAQPVT IREIAEPPKI 850 860 870 880 890 900 RLPRHLRQTY IRKVGEQLNL VVPFQGKPRP QVVWTKGGAP LDTSRVHVRT SDFDTVFFVR 910 920 930 940 950 960 QAARSDSGEY ELSVQIENMK DTATIRIRVV EKAGPPINVM VKEVWGTNAL VEWQAPKDDG 970 980 990 1000 1010 1020 NSEIMGYFVQ KADKKTMEWF NVYERNRHTS CTVSDLIVGN EYYFRVYTEN ICGLSDSPGV 1030 1040 1050 1060 1070 1080 SKNTARILKT GITFKPFEYK EHDFRMAPKF LTPLIDRVVV AGYSAALNCA VRGHPKPKVV 1090 1100 1110 1120 1130 1140 WMKNKMEIRE DPKFLITNYQ GVLTLNIRRP SPFDAGTYTC RAVNELGEAL AECKLEVRVP Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment." Weber F.E., Vaughan K.T., Reinach F.C., Fischman D.A. Eur. J. Biochem. 216:661-669(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal skeletal muscle.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Solution structure of the first, third and 6th Ig-like domains from human myosin-binding protein c, fast-type." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 44-157; 345-438 AND 825-935.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X73113 mRNA. Translation: CAA51544.1.
AC020909 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71866.1.
BC130536 mRNA. Translation: AAI30537.1.
BC136389 mRNA. Translation: AAI36390.1.

IPI

IPI00030104.

PIR

S36845.

RefSeq

NP_004524.3. NM_004533.3.

UniGene

Hs.85937.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E7C	NMR	-	A	825-934	[»]
2EDK	NMR	-	A	345-438	[»]
2EDN	NMR	-	A	47-157	[»]

ProteinModelPortal

Q14324.

SMR

Q14324. Positions 44-157, 213-246, 253-1035, 1046-1128.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14324. 30 interactions.

MINT

MINT-6174870.

STRING

9606.ENSP00000350332.

PTM databases

PhosphoSite

Q14324.

Polymorphism databases

DMDM

296439237.

2D gel databases

REPRODUCTION-2DPAGE

IPI00030104.

Proteomic databases

PaxDb

Q14324.

PRIDE

Q14324.

Protocols and materials databases

DNASU

4606.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000357701; ENSP00000350332; ENSG00000086967.

GeneID

4606.

KEGG

hsa:4606.

UCSC

uc002psf.2. human.

Organism-specific databases

CTD

4606.

GeneCards

GC19P050936.

H-InvDB

HIX0040195.

HGNC

HGNC:7550. MYBPC2.

HPA

HPA042991.
HPA046745.

MIM

160793. gene.

neXtProt

NX_Q14324.

PharmGKB

PA31350.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG146887.

HOGENOM

HOG000220906.

HOVERGEN

HBG052560.

InParanoid

Q14324.

KO

K12558.

OMA

TGKWYKN.

OrthoDB

EOG41RPT6.

Enzyme and pathway databases

Reactome

REACT_17044. Muscle contraction.

Gene expression databases

Bgee

Q14324.

CleanEx

HS_MYBPC2.

Genevestigator

Q14324.

GermOnline

ENSG00000086967. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 10 hits.

InterPro

IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]

Pfam

PF00041. fn3. 3 hits.
PF07679. I-set. 6 hits.
[Graphical view]

SMART

SM00060. FN3. 3 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 1 hit.
[Graphical view]

SUPFAM

SSF49265. FN_III-like. 3 hits.

PROSITE

PS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MYBPC2. human.

EvolutionaryTrace

Q14324.

GenomeRNAi

4606.

NextBio

17728.

SOURCE

Search...

Entry information

Entry name

MYPC2_HUMAN

Accession

Primary (citable) accession number: Q14324
Secondary accession number(s): A1L4G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.