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Q14296 (FASTK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fas-activated serine/threonine kinase

Short name=FAST kinase
EC=2.7.11.8
Gene names
Name:FASTK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor. Ref.1 Ref.7

Catalytic activity

ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].

Subunit structure

Interacts with TIA-1 and TIAR. Ref.7

Tissue specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational modification

Autophosphorylated on serine/threonine residues. Activated by dephosphorylation. Ref.1

Sequence similarities

Belongs to the FAST protein kinase family.

Contains 1 RAP domain.

Sequence caution

The sequence BAD93020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q14296-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q14296-2)

The sequence of this isoform differs from the canonical sequence as follows:
     28-28: W → C
     29-169: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q14296-3)

The sequence of this isoform differs from the canonical sequence as follows:
     202-228: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Fas-activated serine/threonine kinase
PRO_0000087196

Regions

Domain477 – 53559RAP
Compositional bias189 – 1957Poly-Pro

Natural variations

Alternative sequence281W → C in isoform 2.
VSP_042746
Alternative sequence29 – 169141Missing in isoform 2.
VSP_042747
Alternative sequence202 – 22827Missing in isoform 3.
VSP_044811
Natural variant4241V → L in a lung adenocarcinoma sample; somatic mutation. Ref.8
VAR_042200
Natural variant4361A → V.
Corresponds to variant rs2288648 [ dbSNP | Ensembl ].
VAR_021970

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 76D38A211B37304A

FASTA54961,104
        10         20         30         40         50         60 
MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP 

        70         80         90        100        110        120 
CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR 

       130        140        150        160        170        180 
LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL 

       190        200        210        220        230        240 
EQERRLRLPP KPPPPLQPLL RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM 

       250        260        270        280        290        300 
VLLAQHLARH RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL 

       310        320        330        340        350        360 
ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH ALIVRRYLSL 

       370        380        390        400        410        420 
LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS HKDIVAEGLR QLLGEEKYRQ 

       430        440        450        460        470        480 
DLTVPPGYCT DFLLCASSSG AVLPVRTQDP FLPYPPRSCP QGQAASSATT RDPAQRVVLV 

       490        500        510        520        530        540 
LRERWHFCRD GRVLLGSRAL RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL 


GLRWGPEGG 

« Hide

Isoform 2 [UniParc].

Checksum: 7C5944F3AF49F335
Show »

FASTA40845,852
Isoform 3 [UniParc].

Checksum: 86826CD34032595F
Show »

FASTA52258,181

References

« Hide 'large scale' references
[1]"Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis."
Tian Q., Taupin J.-L., Elledge S., Robertson M., Anderson P.
J. Exp. Med. 182:865-874(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, DEPHOSPHORYLATION.
Tissue: Peripheral blood lymphocyte.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]"Homo sapiens protein coding cDNA."
Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Skin.
[7]"Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing."
Izquierdo J.M., Valcarcel J.
J. Biol. Chem. 282:1539-1543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TIA1 AND TIAR.
[8]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-424.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86779 mRNA. Translation: CAA60448.1.
AK292232 mRNA. Translation: BAF84921.1.
AB209783 mRNA. Translation: BAD93020.1. Different initiation.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54039.1.
CH471173 Genomic DNA. Translation: EAW54043.1.
BC011770 mRNA. Translation: AAH11770.1.
BC039026 mRNA. Translation: AAH39026.1.
CCDSCCDS59088.1. [Q14296-3]
CCDS5918.1. [Q14296-1]
CCDS5919.1. [Q14296-2]
PIRI37386.
RefSeqNP_001245390.1. NM_001258461.1. [Q14296-3]
NP_006703.1. NM_006712.4. [Q14296-1]
NP_148936.2. NM_033015.3. [Q14296-2]
UniGeneHs.647094.

3D structure databases

ProteinModelPortalQ14296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116126. 5 interactions.
IntActQ14296. 3 interactions.
STRING9606.ENSP00000297532.

PTM databases

PhosphoSiteQ14296.

Polymorphism databases

DMDM13124202.

Proteomic databases

MaxQBQ14296.
PaxDbQ14296.
PRIDEQ14296.

Protocols and materials databases

DNASU10922.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000297532; ENSP00000297532; ENSG00000164896. [Q14296-1]
ENST00000353841; ENSP00000324817; ENSG00000164896. [Q14296-2]
ENST00000482571; ENSP00000418516; ENSG00000164896. [Q14296-3]
GeneID10922.
KEGGhsa:10922.
UCSCuc003wiw.1. human. [Q14296-1]
uc003wiy.2. human. [Q14296-2]

Organism-specific databases

CTD10922.
GeneCardsGC07M150773.
HGNCHGNC:24676. FASTK.
HPACAB032539.
HPA031621.
MIM606965. gene.
neXtProtNX_Q14296.
PharmGKBPA142671775.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47372.
HOGENOMHOG000013156.
HOVERGENHBG051555.
InParanoidQ14296.
KOK08290.
OMAQNPTKVR.
OrthoDBEOG7TBC2C.
PhylomeDBQ14296.
TreeFamTF331796.

Enzyme and pathway databases

BRENDA2.7.11.8. 2681.

Gene expression databases

ArrayExpressQ14296.
BgeeQ14296.
CleanExHS_FASTK.
GenevestigatorQ14296.

Family and domain databases

InterProIPR013579. FAST_2.
IPR010622. FAST_Leu-rich.
IPR013584. RAP.
IPR015715. Ser/Thr_kinase_FAST.
[Graphical view]
PANTHERPTHR21228:SF4. PTHR21228:SF4. 1 hit.
PfamPF06743. FAST_1. 1 hit.
PF08368. FAST_2. 1 hit.
PF08373. RAP. 1 hit.
[Graphical view]
SMARTSM00952. RAP. 1 hit.
[Graphical view]
PROSITEPS51286. RAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiFASTK.
GenomeRNAi10922.
NextBio41493.
PROQ14296.
SOURCESearch...

Entry information

Entry nameFASTK_HUMAN
AccessionPrimary (citable) accession number: Q14296
Secondary accession number(s): A8K867 expand/collapse secondary AC list , F8VTW9, Q59EM8, Q8IVA0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM