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Protein

Fas-activated serine/threonine kinase

Gene

FASTK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.2 Publications
Isoform 4: Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels.1 Publication

Catalytic activityi

ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].

GO - Molecular functioni

GO - Biological processi

  • apoptotic signaling pathway Source: ProtInc
  • protein phosphorylation Source: UniProtKB
  • regulation of RNA splicing Source: UniProtKB

Keywordsi

Molecular functionKinase, RNA-binding, Serine/threonine-protein kinase, Transferase
Biological processApoptosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.8. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Fas-activated serine/threonine kinase (EC:2.7.11.8)
Short name:
FAST kinase
Gene namesi
Name:FASTK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000164896.19.
HGNCiHGNC:24676. FASTK.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35M → G: Abolishes isoform 4 expression. 1 Publication1

Organism-specific databases

DisGeNETi10922.
OpenTargetsiENSG00000164896.
PharmGKBiPA142671775.

Polymorphism and mutation databases

BioMutaiFASTK.
DMDMi13124202.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000871961 – 549Fas-activated serine/threonine kinaseAdd BLAST549

Post-translational modificationi

Autophosphorylated on serine/threonine residues. Activated by dephosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ14296.
PaxDbiQ14296.
PeptideAtlasiQ14296.
PRIDEiQ14296.

PTM databases

iPTMnetiQ14296.
PhosphoSitePlusiQ14296.

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Gene expression databases

BgeeiENSG00000164896.
CleanExiHS_FASTK.
ExpressionAtlasiQ14296. baseline and differential.
GenevisibleiQ14296. HS.

Organism-specific databases

HPAiCAB032539.
HPA031621.

Interactioni

Subunit structurei

Interacts with TIA-1 and TIAR.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116126. 8 interactors.
IntActiQ14296. 9 interactors.
STRINGi9606.ENSP00000297532.

Structurei

3D structure databases

ProteinModelPortaliQ14296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini477 – 535RAPPROSITE-ProRule annotationAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi189 – 195Poly-Pro7

Domaini

The RAP domain is essential for RNA-binding.1 Publication

Sequence similaritiesi

Belongs to the FAST protein kinase family.Curated

Phylogenomic databases

eggNOGiENOG410IFN3. Eukaryota.
ENOG410ZQD8. LUCA.
GeneTreeiENSGT00530000063266.
HOGENOMiHOG000013156.
HOVERGENiHBG051555.
InParanoidiQ14296.
KOiK08290.
OMAiQNPTKVR.
OrthoDBiEOG091G0WV6.
PhylomeDBiQ14296.
TreeFamiTF331796.

Family and domain databases

InterProiView protein in InterPro
IPR013579. FAST_2.
IPR010622. FAST_Leu-rich.
IPR013584. RAP.
IPR015715. Ser/Thr_kinase_FAST.
PANTHERiPTHR21228:SF41. PTHR21228:SF41. 1 hit.
PfamiView protein in Pfam
PF06743. FAST_1. 1 hit.
PF08368. FAST_2. 1 hit.
PF08373. RAP. 1 hit.
SMARTiView protein in SMART
SM00952. RAP. 1 hit.
PROSITEiView protein in PROSITE
PS51286. RAP. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q14296-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS
60 70 80 90 100
GLLLIPPVQP CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR
110 120 130 140 150
WLGQNPSKVR AHHYSVALRR LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC
160 170 180 190 200
PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL EQERRLRLPP KPPPPLQPLL
210 220 230 240 250
RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM VLLAQHLARH
260 270 280 290 300
RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL
310 320 330 340 350
ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH
360 370 380 390 400
ALIVRRYLSL LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS
410 420 430 440 450
HKDIVAEGLR QLLGEEKYRQ DLTVPPGYCT DFLLCASSSG AVLPVRTQDP
460 470 480 490 500
FLPYPPRSCP QGQAASSATT RDPAQRVVLV LRERWHFCRD GRVLLGSRAL
510 520 530 540
RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG
Length:549
Mass (Da):61,104
Last modified:November 1, 1996 - v1
Checksum:i76D38A211B37304A
GO
Isoform 2 (identifier: Q14296-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-28: W → C
     29-169: Missing.

Note: No experimental confirmation available.
Show »
Length:408
Mass (Da):45,852
Checksum:i7C5944F3AF49F335
GO
Isoform 3 (identifier: Q14296-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-228: Missing.

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):58,181
Checksum:i86826CD34032595F
GO
Isoform 4 (identifier: Q14296-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Show »
Length:515
Mass (Da):57,576
Checksum:i142C239FE54C31EF
GO

Sequence cautioni

The sequence BAD93020 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042200424V → L in a lung adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs758691941Ensembl.1
Natural variantiVAR_021970436A → V. Corresponds to variant dbSNP:rs2288648Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0582461 – 34Missing in isoform 4. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_04274628W → C in isoform 2. 1 Publication1
Alternative sequenceiVSP_04274729 – 169Missing in isoform 2. 1 PublicationAdd BLAST141
Alternative sequenceiVSP_044811202 – 228Missing in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86779 mRNA. Translation: CAA60448.1.
AK292232 mRNA. Translation: BAF84921.1.
AB209783 mRNA. Translation: BAD93020.1. Different initiation.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54039.1.
CH471173 Genomic DNA. Translation: EAW54043.1.
BC011770 mRNA. Translation: AAH11770.1.
BC039026 mRNA. Translation: AAH39026.1.
CCDSiCCDS59088.1. [Q14296-3]
CCDS5918.1. [Q14296-1]
CCDS5919.1. [Q14296-2]
PIRiI37386.
RefSeqiNP_001245390.1. NM_001258461.1. [Q14296-3]
NP_006703.1. NM_006712.4. [Q14296-1]
NP_148936.2. NM_033015.3. [Q14296-2]
XP_005249989.1. XM_005249932.1. [Q14296-4]
XP_005249990.1. XM_005249933.3. [Q14296-4]
UniGeneiHs.647094.

Genome annotation databases

EnsembliENST00000297532; ENSP00000297532; ENSG00000164896. [Q14296-1]
ENST00000353841; ENSP00000324817; ENSG00000164896. [Q14296-2]
ENST00000482571; ENSP00000418516; ENSG00000164896. [Q14296-3]
GeneIDi10922.
KEGGihsa:10922.
UCSCiuc003wix.3. human. [Q14296-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiFASTK_HUMAN
AccessioniPrimary (citable) accession number: Q14296
Secondary accession number(s): A8K867
, F8VTW9, Q59EM8, Q8IVA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families