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Protein

Fas-activated serine/threonine kinase

Gene

FASTK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.2 Publications

Catalytic activityi

ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. Fas-activated serine/threonine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic signaling pathway Source: ProtInc
  2. protein phosphorylation Source: UniProtKB
  3. regulation of RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.8. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Fas-activated serine/threonine kinase (EC:2.7.11.8)
Short name:
FAST kinase
Gene namesi
Name:FASTK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:24676. FASTK.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671775.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Fas-activated serine/threonine kinasePRO_0000087196Add
BLAST

Post-translational modificationi

Autophosphorylated on serine/threonine residues. Activated by dephosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ14296.
PaxDbiQ14296.
PRIDEiQ14296.

PTM databases

PhosphoSiteiQ14296.

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Gene expression databases

BgeeiQ14296.
CleanExiHS_FASTK.
GenevestigatoriQ14296.

Organism-specific databases

HPAiCAB032539.
HPA031621.

Interactioni

Subunit structurei

Interacts with TIA-1 and TIAR.1 Publication

Protein-protein interaction databases

BioGridi116126. 7 interactions.
IntActiQ14296. 3 interactions.
STRINGi9606.ENSP00000297532.

Structurei

3D structure databases

ProteinModelPortaliQ14296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini477 – 53559RAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi189 – 1957Poly-Pro

Sequence similaritiesi

Belongs to the FAST protein kinase family.Curated
Contains 1 RAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG47372.
GeneTreeiENSGT00530000063266.
HOGENOMiHOG000013156.
HOVERGENiHBG051555.
InParanoidiQ14296.
KOiK08290.
OMAiQNPTKVR.
OrthoDBiEOG7TBC2C.
PhylomeDBiQ14296.
TreeFamiTF331796.

Family and domain databases

InterProiIPR013579. FAST_2.
IPR010622. FAST_Leu-rich.
IPR013584. RAP.
IPR015715. Ser/Thr_kinase_FAST.
[Graphical view]
PANTHERiPTHR21228:SF4. PTHR21228:SF4. 1 hit.
PfamiPF06743. FAST_1. 1 hit.
PF08368. FAST_2. 1 hit.
PF08373. RAP. 1 hit.
[Graphical view]
SMARTiSM00952. RAP. 1 hit.
[Graphical view]
PROSITEiPS51286. RAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14296-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS
60 70 80 90 100
GLLLIPPVQP CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR
110 120 130 140 150
WLGQNPSKVR AHHYSVALRR LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC
160 170 180 190 200
PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL EQERRLRLPP KPPPPLQPLL
210 220 230 240 250
RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM VLLAQHLARH
260 270 280 290 300
RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL
310 320 330 340 350
ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH
360 370 380 390 400
ALIVRRYLSL LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS
410 420 430 440 450
HKDIVAEGLR QLLGEEKYRQ DLTVPPGYCT DFLLCASSSG AVLPVRTQDP
460 470 480 490 500
FLPYPPRSCP QGQAASSATT RDPAQRVVLV LRERWHFCRD GRVLLGSRAL
510 520 530 540
RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG
Length:549
Mass (Da):61,104
Last modified:November 1, 1996 - v1
Checksum:i76D38A211B37304A
GO
Isoform 2 (identifier: Q14296-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-28: W → C
     29-169: Missing.

Note: No experimental confirmation available.

Show »
Length:408
Mass (Da):45,852
Checksum:i7C5944F3AF49F335
GO
Isoform 3 (identifier: Q14296-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-228: Missing.

Note: No experimental confirmation available.

Show »
Length:522
Mass (Da):58,181
Checksum:i86826CD34032595F
GO

Sequence cautioni

The sequence BAD93020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti424 – 4241V → L in a lung adenocarcinoma sample; somatic mutation. 1 Publication
VAR_042200
Natural varianti436 – 4361A → V.
Corresponds to variant rs2288648 [ dbSNP | Ensembl ].
VAR_021970

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 281W → C in isoform 2. 1 PublicationVSP_042746
Alternative sequencei29 – 169141Missing in isoform 2. 1 PublicationVSP_042747Add
BLAST
Alternative sequencei202 – 22827Missing in isoform 3. 1 PublicationVSP_044811Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86779 mRNA. Translation: CAA60448.1.
AK292232 mRNA. Translation: BAF84921.1.
AB209783 mRNA. Translation: BAD93020.1. Different initiation.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54039.1.
CH471173 Genomic DNA. Translation: EAW54043.1.
BC011770 mRNA. Translation: AAH11770.1.
BC039026 mRNA. Translation: AAH39026.1.
CCDSiCCDS59088.1. [Q14296-3]
CCDS5918.1. [Q14296-1]
CCDS5919.1. [Q14296-2]
PIRiI37386.
RefSeqiNP_001245390.1. NM_001258461.1. [Q14296-3]
NP_006703.1. NM_006712.4. [Q14296-1]
NP_148936.2. NM_033015.3. [Q14296-2]
UniGeneiHs.647094.

Genome annotation databases

EnsembliENST00000297532; ENSP00000297532; ENSG00000164896. [Q14296-1]
ENST00000353841; ENSP00000324817; ENSG00000164896. [Q14296-2]
ENST00000482571; ENSP00000418516; ENSG00000164896. [Q14296-3]
GeneIDi10922.
KEGGihsa:10922.
UCSCiuc003wiw.1. human. [Q14296-1]
uc003wiy.2. human. [Q14296-2]

Polymorphism databases

DMDMi13124202.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86779 mRNA. Translation: CAA60448.1.
AK292232 mRNA. Translation: BAF84921.1.
AB209783 mRNA. Translation: BAD93020.1. Different initiation.
AC010973 Genomic DNA. No translation available.
CH471173 Genomic DNA. Translation: EAW54039.1.
CH471173 Genomic DNA. Translation: EAW54043.1.
BC011770 mRNA. Translation: AAH11770.1.
BC039026 mRNA. Translation: AAH39026.1.
CCDSiCCDS59088.1. [Q14296-3]
CCDS5918.1. [Q14296-1]
CCDS5919.1. [Q14296-2]
PIRiI37386.
RefSeqiNP_001245390.1. NM_001258461.1. [Q14296-3]
NP_006703.1. NM_006712.4. [Q14296-1]
NP_148936.2. NM_033015.3. [Q14296-2]
UniGeneiHs.647094.

3D structure databases

ProteinModelPortaliQ14296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116126. 7 interactions.
IntActiQ14296. 3 interactions.
STRINGi9606.ENSP00000297532.

PTM databases

PhosphoSiteiQ14296.

Polymorphism databases

DMDMi13124202.

Proteomic databases

MaxQBiQ14296.
PaxDbiQ14296.
PRIDEiQ14296.

Protocols and materials databases

DNASUi10922.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297532; ENSP00000297532; ENSG00000164896. [Q14296-1]
ENST00000353841; ENSP00000324817; ENSG00000164896. [Q14296-2]
ENST00000482571; ENSP00000418516; ENSG00000164896. [Q14296-3]
GeneIDi10922.
KEGGihsa:10922.
UCSCiuc003wiw.1. human. [Q14296-1]
uc003wiy.2. human. [Q14296-2]

Organism-specific databases

CTDi10922.
GeneCardsiGC07M150773.
HGNCiHGNC:24676. FASTK.
HPAiCAB032539.
HPA031621.
MIMi606965. gene.
neXtProtiNX_Q14296.
PharmGKBiPA142671775.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG47372.
GeneTreeiENSGT00530000063266.
HOGENOMiHOG000013156.
HOVERGENiHBG051555.
InParanoidiQ14296.
KOiK08290.
OMAiQNPTKVR.
OrthoDBiEOG7TBC2C.
PhylomeDBiQ14296.
TreeFamiTF331796.

Enzyme and pathway databases

BRENDAi2.7.11.8. 2681.

Miscellaneous databases

GeneWikiiFASTK.
GenomeRNAii10922.
NextBioi41493.
PROiQ14296.
SOURCEiSearch...

Gene expression databases

BgeeiQ14296.
CleanExiHS_FASTK.
GenevestigatoriQ14296.

Family and domain databases

InterProiIPR013579. FAST_2.
IPR010622. FAST_Leu-rich.
IPR013584. RAP.
IPR015715. Ser/Thr_kinase_FAST.
[Graphical view]
PANTHERiPTHR21228:SF4. PTHR21228:SF4. 1 hit.
PfamiPF06743. FAST_1. 1 hit.
PF08368. FAST_2. 1 hit.
PF08373. RAP. 1 hit.
[Graphical view]
SMARTiSM00952. RAP. 1 hit.
[Graphical view]
PROSITEiPS51286. RAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis."
    Tian Q., Taupin J.-L., Elledge S., Robertson M., Anderson P.
    J. Exp. Med. 182:865-874(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, DEPHOSPHORYLATION.
    Tissue: Peripheral blood lymphocyte.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "Homo sapiens protein coding cDNA."
    Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Skin.
  7. "Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing."
    Izquierdo J.M., Valcarcel J.
    J. Biol. Chem. 282:1539-1543(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TIA1 AND TIAR.
  8. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-424.

Entry informationi

Entry nameiFASTK_HUMAN
AccessioniPrimary (citable) accession number: Q14296
Secondary accession number(s): A8K867
, F8VTW9, Q59EM8, Q8IVA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.