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Q14296

- FASTK_HUMAN

UniProt

Q14296 - FASTK_HUMAN

Protein

Fas-activated serine/threonine kinase

Gene

FASTK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.2 Publications

    Catalytic activityi

    ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. Fas-activated serine/threonine kinase activity Source: UniProtKB-EC
    3. protein binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic signaling pathway Source: ProtInc
    2. protein phosphorylation Source: UniProtKB
    3. regulation of RNA splicing Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.8. 2681.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fas-activated serine/threonine kinase (EC:2.7.11.8)
    Short name:
    FAST kinase
    Gene namesi
    Name:FASTK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:24676. FASTK.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671775.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Fas-activated serine/threonine kinasePRO_0000087196Add
    BLAST

    Post-translational modificationi

    Autophosphorylated on serine/threonine residues. Activated by dephosphorylation.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ14296.
    PaxDbiQ14296.
    PRIDEiQ14296.

    PTM databases

    PhosphoSiteiQ14296.

    Expressioni

    Tissue specificityi

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

    Gene expression databases

    ArrayExpressiQ14296.
    BgeeiQ14296.
    CleanExiHS_FASTK.
    GenevestigatoriQ14296.

    Organism-specific databases

    HPAiCAB032539.
    HPA031621.

    Interactioni

    Subunit structurei

    Interacts with TIA-1 and TIAR.1 Publication

    Protein-protein interaction databases

    BioGridi116126. 6 interactions.
    IntActiQ14296. 3 interactions.
    STRINGi9606.ENSP00000297532.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini477 – 53559RAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi189 – 1957Poly-Pro

    Sequence similaritiesi

    Belongs to the FAST protein kinase family.Curated
    Contains 1 RAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG47372.
    HOGENOMiHOG000013156.
    HOVERGENiHBG051555.
    InParanoidiQ14296.
    KOiK08290.
    OMAiQNPTKVR.
    OrthoDBiEOG7TBC2C.
    PhylomeDBiQ14296.
    TreeFamiTF331796.

    Family and domain databases

    InterProiIPR013579. FAST_2.
    IPR010622. FAST_Leu-rich.
    IPR013584. RAP.
    IPR015715. Ser/Thr_kinase_FAST.
    [Graphical view]
    PANTHERiPTHR21228:SF4. PTHR21228:SF4. 1 hit.
    PfamiPF06743. FAST_1. 1 hit.
    PF08368. FAST_2. 1 hit.
    PF08373. RAP. 1 hit.
    [Graphical view]
    SMARTiSM00952. RAP. 1 hit.
    [Graphical view]
    PROSITEiPS51286. RAP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14296-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS    50
    GLLLIPPVQP CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR 100
    WLGQNPSKVR AHHYSVALRR LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC 150
    PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL EQERRLRLPP KPPPPLQPLL 200
    RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM VLLAQHLARH 250
    RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL 300
    ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH 350
    ALIVRRYLSL LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS 400
    HKDIVAEGLR QLLGEEKYRQ DLTVPPGYCT DFLLCASSSG AVLPVRTQDP 450
    FLPYPPRSCP QGQAASSATT RDPAQRVVLV LRERWHFCRD GRVLLGSRAL 500
    RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG 549
    Length:549
    Mass (Da):61,104
    Last modified:November 1, 1996 - v1
    Checksum:i76D38A211B37304A
    GO
    Isoform 2 (identifier: Q14296-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         28-28: W → C
         29-169: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:408
    Mass (Da):45,852
    Checksum:i7C5944F3AF49F335
    GO
    Isoform 3 (identifier: Q14296-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         202-228: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:522
    Mass (Da):58,181
    Checksum:i86826CD34032595F
    GO

    Sequence cautioni

    The sequence BAD93020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti424 – 4241V → L in a lung adenocarcinoma sample; somatic mutation. 1 Publication
    VAR_042200
    Natural varianti436 – 4361A → V.
    Corresponds to variant rs2288648 [ dbSNP | Ensembl ].
    VAR_021970

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei28 – 281W → C in isoform 2. 1 PublicationVSP_042746
    Alternative sequencei29 – 169141Missing in isoform 2. 1 PublicationVSP_042747Add
    BLAST
    Alternative sequencei202 – 22827Missing in isoform 3. 1 PublicationVSP_044811Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86779 mRNA. Translation: CAA60448.1.
    AK292232 mRNA. Translation: BAF84921.1.
    AB209783 mRNA. Translation: BAD93020.1. Different initiation.
    AC010973 Genomic DNA. No translation available.
    CH471173 Genomic DNA. Translation: EAW54039.1.
    CH471173 Genomic DNA. Translation: EAW54043.1.
    BC011770 mRNA. Translation: AAH11770.1.
    BC039026 mRNA. Translation: AAH39026.1.
    CCDSiCCDS59088.1. [Q14296-3]
    CCDS5918.1. [Q14296-1]
    CCDS5919.1. [Q14296-2]
    PIRiI37386.
    RefSeqiNP_001245390.1. NM_001258461.1. [Q14296-3]
    NP_006703.1. NM_006712.4. [Q14296-1]
    NP_148936.2. NM_033015.3. [Q14296-2]
    UniGeneiHs.647094.

    Genome annotation databases

    EnsembliENST00000297532; ENSP00000297532; ENSG00000164896. [Q14296-1]
    ENST00000353841; ENSP00000324817; ENSG00000164896. [Q14296-2]
    ENST00000482571; ENSP00000418516; ENSG00000164896. [Q14296-3]
    GeneIDi10922.
    KEGGihsa:10922.
    UCSCiuc003wiw.1. human. [Q14296-1]
    uc003wiy.2. human. [Q14296-2]

    Polymorphism databases

    DMDMi13124202.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86779 mRNA. Translation: CAA60448.1 .
    AK292232 mRNA. Translation: BAF84921.1 .
    AB209783 mRNA. Translation: BAD93020.1 . Different initiation.
    AC010973 Genomic DNA. No translation available.
    CH471173 Genomic DNA. Translation: EAW54039.1 .
    CH471173 Genomic DNA. Translation: EAW54043.1 .
    BC011770 mRNA. Translation: AAH11770.1 .
    BC039026 mRNA. Translation: AAH39026.1 .
    CCDSi CCDS59088.1. [Q14296-3 ]
    CCDS5918.1. [Q14296-1 ]
    CCDS5919.1. [Q14296-2 ]
    PIRi I37386.
    RefSeqi NP_001245390.1. NM_001258461.1. [Q14296-3 ]
    NP_006703.1. NM_006712.4. [Q14296-1 ]
    NP_148936.2. NM_033015.3. [Q14296-2 ]
    UniGenei Hs.647094.

    3D structure databases

    ProteinModelPortali Q14296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116126. 6 interactions.
    IntActi Q14296. 3 interactions.
    STRINGi 9606.ENSP00000297532.

    PTM databases

    PhosphoSitei Q14296.

    Polymorphism databases

    DMDMi 13124202.

    Proteomic databases

    MaxQBi Q14296.
    PaxDbi Q14296.
    PRIDEi Q14296.

    Protocols and materials databases

    DNASUi 10922.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000297532 ; ENSP00000297532 ; ENSG00000164896 . [Q14296-1 ]
    ENST00000353841 ; ENSP00000324817 ; ENSG00000164896 . [Q14296-2 ]
    ENST00000482571 ; ENSP00000418516 ; ENSG00000164896 . [Q14296-3 ]
    GeneIDi 10922.
    KEGGi hsa:10922.
    UCSCi uc003wiw.1. human. [Q14296-1 ]
    uc003wiy.2. human. [Q14296-2 ]

    Organism-specific databases

    CTDi 10922.
    GeneCardsi GC07M150773.
    HGNCi HGNC:24676. FASTK.
    HPAi CAB032539.
    HPA031621.
    MIMi 606965. gene.
    neXtProti NX_Q14296.
    PharmGKBi PA142671775.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47372.
    HOGENOMi HOG000013156.
    HOVERGENi HBG051555.
    InParanoidi Q14296.
    KOi K08290.
    OMAi QNPTKVR.
    OrthoDBi EOG7TBC2C.
    PhylomeDBi Q14296.
    TreeFami TF331796.

    Enzyme and pathway databases

    BRENDAi 2.7.11.8. 2681.

    Miscellaneous databases

    GeneWikii FASTK.
    GenomeRNAii 10922.
    NextBioi 41493.
    PROi Q14296.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14296.
    Bgeei Q14296.
    CleanExi HS_FASTK.
    Genevestigatori Q14296.

    Family and domain databases

    InterProi IPR013579. FAST_2.
    IPR010622. FAST_Leu-rich.
    IPR013584. RAP.
    IPR015715. Ser/Thr_kinase_FAST.
    [Graphical view ]
    PANTHERi PTHR21228:SF4. PTHR21228:SF4. 1 hit.
    Pfami PF06743. FAST_1. 1 hit.
    PF08368. FAST_2. 1 hit.
    PF08373. RAP. 1 hit.
    [Graphical view ]
    SMARTi SM00952. RAP. 1 hit.
    [Graphical view ]
    PROSITEi PS51286. RAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis."
      Tian Q., Taupin J.-L., Elledge S., Robertson M., Anderson P.
      J. Exp. Med. 182:865-874(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, DEPHOSPHORYLATION.
      Tissue: Peripheral blood lymphocyte.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    3. "Homo sapiens protein coding cDNA."
      Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Brain.
    4. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Skin.
    7. "Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing."
      Izquierdo J.M., Valcarcel J.
      J. Biol. Chem. 282:1539-1543(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TIA1 AND TIAR.
    8. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-424.

    Entry informationi

    Entry nameiFASTK_HUMAN
    AccessioniPrimary (citable) accession number: Q14296
    Secondary accession number(s): A8K867
    , F8VTW9, Q59EM8, Q8IVA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3