Q14296 (FASTK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fas-activated serine/threonine kinase
Short name=FAST kinase
EC=2.7.11.8

Gene names

Name:

FASTK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Fas-mediated apoptosis is characterized by FASTK-dephosphorylation and TIA-1-phosphorylation. Both are activated and FASTK-mediated TIA-1 activation play a key role in apoptosis.
Catalytic activity	ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].
Subunit structure	Interacts with TIA-1.
Tissue specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post-translational modification	Phosphorylated on serine/threonine.
Sequence similarities	Belongs to the FAST protein kinase family. Contains 1 RAP domain.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis by extracellular signals Traceable author statement. Source: ProtInc regulation of RNA splicing Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Fas-activated serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Fas-activated serine/threonine kinase		PRO_0000087196
Regions
	Domain	477 – 535	59	RAP
	Compositional bias	189 – 195	7	Poly-Pro
Natural variations
	Natural variant	424	1	V → L in a lung adenocarcinoma sample; somatic mutation. Ref.5		VAR_042200
	Natural variant	436	1	A → V. Corresponds to variant rs2288648 [ dbSNP | Ensembl ].		VAR_021970

Sequences

	Sequence		Length	Mass (Da)
	Q14296 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 76D38A211B37304A	FASTA	549	61,104
	10 20 30 40 50 60 MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP 70 80 90 100 110 120 CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR 130 140 150 160 170 180 LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL 190 200 210 220 230 240 EQERRLRLPP KPPPPLQPLL RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM 250 260 270 280 290 300 VLLAQHLARH RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL 310 320 330 340 350 360 ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH ALIVRRYLSL 370 380 390 400 410 420 LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS HKDIVAEGLR QLLGEEKYRQ 430 440 450 460 470 480 DLTVPPGYCT DFLLCASSSG AVLPVRTQDP FLPYPPRSCP QGQAASSATT RDPAQRVVLV 490 500 510 520 530 540 LRERWHFCRD GRVLLGSRAL RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis." Tian Q., Taupin J.-L., Elledge S., Robertson M., Anderson P. J. Exp. Med. 182:865-874(1995) [PubMed: 7544399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood lymphocyte.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-424.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86779 mRNA. Translation: CAA60448.1. AK292232 mRNA. Translation: BAF84921.1. CH471173 Genomic DNA. Translation: EAW54039.1. BC011770 mRNA. Translation: AAH11770.1.
IPI	IPI00029714.
PIR	I37386.
RefSeq	NP_006703.1. NM_006712.3. NP_148936.2. NM_033015.2.
UniGene	Hs.647094.
3D structure databases
ProteinModelPortal	Q14296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14296. 1 interaction.
MINT	MINT-7242605.
STRING	Q14296.
PTM databases
PhosphoSite	Q14296.
Polymorphism databases
DMDM	13124202.
Proteomic databases
PRIDE	Q14296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297532; ENSP00000297532; ENSG00000164896.
GeneID	10922.
KEGG	hsa:10922.
UCSC	uc003wix.1. human.
Organism-specific databases
CTD	10922.
GeneCards	GC07M150773.
H-InvDB	HIX0007223.
HGNC	HGNC:24676. FASTK.
HPA	CAB032539. HPA031621.
MIM	606965. gene.
neXtProt	NX_Q14296.
PharmGKB	PA142671775.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14591.
HOGENOM	HBG717199.
HOVERGEN	HBG051555.
InParanoid	Q14296.
OMA	TIHVCLH.
OrthoDB	EOG4RR6HN.
PhylomeDB	Q14296.
Enzyme and pathway databases
BRENDA	2.7.11.8. 2681.
Gene expression databases
ArrayExpress	Q14296.
Bgee	Q14296.
CleanEx	HS_FASTK.
Genevestigator	Q14296.
GermOnline	ENSG00000164896. Homo sapiens.
Family and domain databases
InterPro	IPR013579. FAST_2. IPR010622. FAST_Leu-rich. IPR013584. RAP. IPR015715. Ser/Thr_kinase_FAST. [Graphical view]
KO	K08290.
PANTHER	PTHR21228:SF4. FAST_kinase. 1 hit.
Pfam	PF06743. FAST_1. 1 hit. PF08368. FAST_2. 1 hit. PF08373. RAP. 1 hit. [Graphical view]
SMART	SM00952. RAP. 1 hit. [Graphical view]
PROSITE	PS51286. RAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	41493.
SOURCE	Search...

Entry information

Entry name

FASTK_HUMAN

Accession

Primary (citable) accession number: Q14296
Secondary accession number(s): A8K867

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.