Q14296 (FASTK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fas-activated serine/threonine kinase
Short name=FAST kinase
EC=2.7.11.8

Gene names

Name:

FASTK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor. Ref.1 Ref.7
Catalytic activity	ATP + [Fas-activated serine/threonine protein] = ADP + [Fas-activated serine/threonine phosphoprotein].
Subunit structure	Interacts with TIA-1 and TIAR. Ref.7
Tissue specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post-translational modification	Autophosphorylated on serine/threonine residues. Activated by dephosphorylation. Ref.1
Sequence similarities	Belongs to the FAST protein kinase family. Contains 1 RAP domain.
Sequence caution	The sequence BAD93020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic signaling pathway Traceable author statement Ref.1. Source: ProtInc regulation of RNA splicing Inferred from direct assay Ref.7. Source: UniProtKB
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Fas-activated serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14296-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14296-2)

The sequence of this isoform differs from the canonical sequence as follows:
     28-28: W → C
     29-169: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q14296-3)

The sequence of this isoform differs from the canonical sequence as follows:
     202-228: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Fas-activated serine/threonine kinase		PRO_0000087196
Regions
	Domain	477 – 535	59	RAP
	Compositional bias	189 – 195	7	Poly-Pro
Natural variations
	Alternative sequence	28	1	W → C in isoform 2.		VSP_042746
	Alternative sequence	29 – 169	141	Missing in isoform 2.		VSP_042747
	Alternative sequence	202 – 228	27	Missing in isoform 3.		VSP_044811
	Natural variant	424	1	V → L in a lung adenocarcinoma sample; somatic mutation. Ref.8		VAR_042200
	Natural variant	436	1	A → V. Corresponds to variant rs2288648 [ dbSNP | Ensembl ].		VAR_021970

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 76D38A211B37304A	FASTA	549	61,104
	10 20 30 40 50 60 MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP 70 80 90 100 110 120 CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR 130 140 150 160 170 180 LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL 190 200 210 220 230 240 EQERRLRLPP KPPPPLQPLL RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM 250 260 270 280 290 300 VLLAQHLARH RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL 310 320 330 340 350 360 ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH ALIVRRYLSL 370 380 390 400 410 420 LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS HKDIVAEGLR QLLGEEKYRQ 430 440 450 460 470 480 DLTVPPGYCT DFLLCASSSG AVLPVRTQDP FLPYPPRSCP QGQAASSATT RDPAQRVVLV 490 500 510 520 530 540 LRERWHFCRD GRVLLGSRAL RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG
	Isoform 2 [UniParc]. Checksum: 7C5944F3AF49F335	FASTA	408	45,852
	10 20 30 40 50 60 MRRPRGEPGP RAPRPTEGAT CAGPGESCFP SDGPLVCALE QERRLRLPPK PPPPLQPLLR 70 80 90 100 110 120 GGQGLEAALS CPRFLRYPRQ HLISSLAEAR PEELTPHVMV LLAQHLARHR LREPQLLEAI 130 140 150 160 170 180 AHFLVVQETQ LSSKVVQKLV LPFGRLNYLP LEQQFMPCLE RILAREAGVA PLATVNILMS 190 200 210 220 230 240 LCQLRCLPFR ALHFVFSPGF INYISGTPHA LIVRRYLSLL DTAVELELPG YRGPRLPRRQ 250 260 270 280 290 300 QVPIFPQPLI TDRARCKYSH KDIVAEGLRQ LLGEEKYRQD LTVPPGYCTD FLLCASSSGA 310 320 330 340 350 360 VLPVRTQDPF LPYPPRSCPQ GQAASSATTR DPAQRVVLVL RERWHFCRDG RVLLGSRALR 370 380 390 400 ERHLGLMGYQ LLPLPFEELE SQRGLPQLKS YLRQKLQALG LRWGPEGG
	Isoform 3 [UniParc]. Checksum: 86826CD34032595F	FASTA	522	58,181
	10 20 30 40 50 60 MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP 70 80 90 100 110 120 CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR 130 140 150 160 170 180 LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL 190 200 210 220 230 240 EQERRLRLPP KPPPPLQPLL REARPEELTP HVMVLLAQHL ARHRLREPQL LEAIAHFLVV 250 260 270 280 290 300 QETQLSSKVV QKLVLPFGRL NYLPLEQQFM PCLERILARE AGVAPLATVN ILMSLCQLRC 310 320 330 340 350 360 LPFRALHFVF SPGFINYISG TPHALIVRRY LSLLDTAVEL ELPGYRGPRL PRRQQVPIFP 370 380 390 400 410 420 QPLITDRARC KYSHKDIVAE GLRQLLGEEK YRQDLTVPPG YCTDFLLCAS SSGAVLPVRT 430 440 450 460 470 480 QDPFLPYPPR SCPQGQAASS ATTRDPAQRV VLVLRERWHF CRDGRVLLGS RALRERHLGL 490 500 510 520 MGYQLLPLPF EELESQRGLP QLKSYLRQKL QALGLRWGPE GG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis." Tian Q., Taupin J.-L., Elledge S., Robertson M., Anderson P. J. Exp. Med. 182:865-874(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, DEPHOSPHORYLATION. Tissue: Peripheral blood lymphocyte.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"Homo sapiens protein coding cDNA." Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Skin.
[7]	"Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing." Izquierdo J.M., Valcarcel J. J. Biol. Chem. 282:1539-1543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TIA1 AND TIAR.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-424.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86779 mRNA. Translation: CAA60448.1. AK292232 mRNA. Translation: BAF84921.1. AB209783 mRNA. Translation: BAD93020.1. Different initiation. AC010973 Genomic DNA. No translation available. CH471173 Genomic DNA. Translation: EAW54039.1. CH471173 Genomic DNA. Translation: EAW54043.1. BC011770 mRNA. Translation: AAH11770.1. BC039026 mRNA. Translation: AAH39026.1.
IPI	IPI00029714. IPI00216966. IPI00945628.
PIR	I37386.
RefSeq	NP_001245390.1. NM_001258461.1. NP_006703.1. NM_006712.4. NP_148936.2. NM_033015.3.
UniGene	Hs.647094.
3D structure databases
ProteinModelPortal	Q14296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14296. 3 interactions.
STRING	9606.ENSP00000297532.
PTM databases
PhosphoSite	Q14296.
Polymorphism databases
DMDM	13124202.
Proteomic databases
PaxDb	Q14296.
PRIDE	Q14296.
Protocols and materials databases
DNASU	10922.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297532; ENSP00000297532; ENSG00000164896. ENST00000353841; ENSP00000324817; ENSG00000164896. ENST00000482571; ENSP00000418516; ENSG00000164896.
GeneID	10922.
KEGG	hsa:10922.
UCSC	uc003wiw.1. human. uc003wiy.1. human. uc003wiz.1. human.
Organism-specific databases
CTD	10922.
GeneCards	GC07M150773.
HGNC	HGNC:24676. FASTK.
HPA	CAB032539. HPA031621.
MIM	606965. gene.
neXtProt	NX_Q14296.
PharmGKB	PA142671775.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47372.
HOGENOM	HOG000013156.
HOVERGEN	HBG051555.
InParanoid	Q14296.
KO	K08290.
OMA	QNPTKVR.
OrthoDB	EOG4RR6HN.
PhylomeDB	Q14296.
Enzyme and pathway databases
BRENDA	2.7.11.8. 2681.
Gene expression databases
ArrayExpress	Q14296.
Bgee	Q14296.
CleanEx	HS_FASTK.
Genevestigator	Q14296.
GermOnline	ENSG00000164896. Homo sapiens.
Family and domain databases
InterPro	IPR013579. FAST_2. IPR010622. FAST_Leu-rich. IPR013584. RAP. IPR015715. Ser/Thr_kinase_FAST. [Graphical view]
PANTHER	PTHR21228:SF4. PTHR21228:SF4. 1 hit.
Pfam	PF06743. FAST_1. 1 hit. PF08368. FAST_2. 1 hit. PF08373. RAP. 1 hit. [Graphical view]
SMART	SM00952. RAP. 1 hit. [Graphical view]
PROSITE	PS51286. RAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10922.
NextBio	41493.
SOURCE	Search...

Entry information

Entry name

FASTK_HUMAN

Accession

Primary (citable) accession number: Q14296
Secondary accession number(s): A8K867 , F8VTW9, Q59EM8, Q8IVA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.