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Protein

Protein-tyrosine kinase 2-beta

Gene

PTK2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.25 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation6 Publications

Enzyme regulationi

Activated in response to stimuli that lead to increased intracellular Ca2+ levels; this activation is indirect and may be mediated by calcium-mediated production of reactive oxygen species (ROS). Activated by autophosphorylation at Tyr-402; this creates a binding site for SRC family kinases and leads to phosphorylation at additional tyrosine residues. Phosphorylation at Tyr-402, Tyr-579 and Tyr-580 is required for optimal kinase activity. Inhibited by PF-562,271, BIRB796, PF-4618433 and by PF-431396, PF-2318841 and their derivatives. Inhibited by sulfoximine-substituted trifluoromethylpyrimidines. Inhibited by 4-amino and 5-aryl substituted pyridinone compounds.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei457 – 4571ATP
Active sitei549 – 5491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi431 – 4399ATP
Nucleotide bindingi503 – 5097ATP

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: Alzheimers_University_of_Toronto
  • NMDA glutamate receptor activity Source: Ensembl
  • non-membrane spanning protein tyrosine kinase activity Source: UniProtKB
  • protein tyrosine kinase activity Source: ProtInc
  • receptor binding Source: GO_Central
  • signal transducer activity Source: ProtInc

GO - Biological processi

  • activation of GTPase activity Source: Ensembl
  • activation of Janus kinase activity Source: UniProtKB
  • adaptive immune response Source: UniProtKB-KW
  • angiogenesis Source: GO_Central
  • apoptotic process Source: ProtInc
  • blood vessel endothelial cell migration Source: Ensembl
  • bone resorption Source: UniProtKB
  • cell surface receptor signaling pathway Source: UniProtKB
  • cellular defense response Source: Alzheimers_University_of_Toronto
  • cellular response to fluid shear stress Source: Ensembl
  • cellular response to retinoic acid Source: BHF-UCL
  • chemokine-mediated signaling pathway Source: UniProtKB
  • epidermal growth factor receptor signaling pathway Source: GO_Central
  • focal adhesion assembly Source: Ensembl
  • glial cell proliferation Source: Ensembl
  • innate immune response Source: GO_Central
  • integrin-mediated signaling pathway Source: UniProtKB
  • ionotropic glutamate receptor signaling pathway Source: Alzheimers_University_of_Toronto
  • long term synaptic depression Source: Ensembl
  • long-term synaptic potentiation Source: Alzheimers_University_of_Toronto
  • MAPK cascade Source: Ensembl
  • marginal zone B cell differentiation Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of bone mineralization Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of muscle cell apoptotic process Source: Ensembl
  • negative regulation of myeloid cell differentiation Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: Alzheimers_University_of_Toronto
  • negative regulation of potassium ion transport Source: UniProtKB
  • neuron projection development Source: Ensembl
  • oocyte maturation Source: Ensembl
  • peptidyl-tyrosine autophosphorylation Source: BHF-UCL
  • peptidyl-tyrosine phosphorylation Source: UniProtKB
  • positive regulation of actin filament polymerization Source: UniProtKB
  • positive regulation of angiogenesis Source: Ensembl
  • positive regulation of B cell chemotaxis Source: UniProtKB
  • positive regulation of cell growth Source: Ensembl
  • positive regulation of cell-matrix adhesion Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: Ensembl
  • positive regulation of DNA biosynthetic process Source: Ensembl
  • positive regulation of endothelial cell migration Source: BHF-UCL
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of excitatory postsynaptic potential Source: Alzheimers_University_of_Toronto
  • positive regulation of JNK cascade Source: UniProtKB
  • positive regulation of JUN kinase activity Source: Ensembl
  • positive regulation of neuron projection development Source: BHF-UCL
  • positive regulation of nitric oxide biosynthetic process Source: Ensembl
  • positive regulation of nitric-oxide synthase activity Source: Ensembl
  • positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  • positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
  • positive regulation of protein kinase activity Source: UniProtKB
  • positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: Ensembl
  • positive regulation of synaptic transmission, glutamatergic Source: Alzheimers_University_of_Toronto
  • positive regulation of translation Source: Ensembl
  • protein autophosphorylation Source: UniProtKB
  • protein complex assembly Source: ProtInc
  • protein phosphorylation Source: ProtInc
  • regulation of actin cytoskeleton reorganization Source: UniProtKB
  • regulation of calcium-mediated signaling Source: Ensembl
  • regulation of cell adhesion Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
  • regulation of cGMP biosynthetic process Source: Ensembl
  • regulation of cGMP-mediated signaling Source: Ensembl
  • regulation of establishment of cell polarity Source: UniProtKB
  • regulation of inositol trisphosphate biosynthetic process Source: UniProtKB
  • regulation of macrophage chemotaxis Source: UniProtKB
  • regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: Alzheimers_University_of_Toronto
  • regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: UniProtKB
  • regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
  • response to calcium ion Source: Ensembl
  • response to cAMP Source: Ensembl
  • response to cocaine Source: Ensembl
  • response to drug Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to glucose Source: Ensembl
  • response to hormone Source: Ensembl
  • response to hydrogen peroxide Source: Ensembl
  • response to hypoxia Source: Ensembl
  • response to immobilization stress Source: Ensembl
  • response to lithium ion Source: Ensembl
  • response to mechanical stimulus Source: Ensembl
  • response to osmotic stress Source: Ensembl
  • response to stress Source: ProtInc
  • signal complex assembly Source: ProtInc
  • signal transduction Source: ProtInc
  • sprouting angiogenesis Source: UniProtKB
  • stress fiber assembly Source: Ensembl
  • tumor necrosis factor-mediated signaling pathway Source: UniProtKB
  • vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Adaptive immunity, Angiogenesis, Immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 2681.
ReactomeiR-HSA-391160. Signal regulatory protein (SIRP) family interactions.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-451927. Interleukin-2 signaling.
SignaLinkiQ14289.
SIGNORiQ14289.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine kinase 2-beta (EC:2.7.10.2)
Alternative name(s):
Calcium-dependent tyrosine kinase
Short name:
CADTK
Calcium-regulated non-receptor proline-rich tyrosine kinase
Cell adhesion kinase beta
Short name:
CAK-beta
Short name:
CAKB
Focal adhesion kinase 2
Short name:
FADK 2
Proline-rich tyrosine kinase 2
Related adhesion focal tyrosine kinase
Short name:
RAFTK
Gene namesi
Name:PTK2B
Synonyms:FAK2, PYK2, RAFTK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:9612. PTK2B.

Subcellular locationi

GO - Cellular componenti

  • apical dendrite Source: Alzheimers_University_of_Toronto
  • axon Source: Ensembl
  • cell body Source: Alzheimers_University_of_Toronto
  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: HPA
  • cytoskeleton Source: InterPro
  • cytosol Source: Reactome
  • dendrite Source: Alzheimers_University_of_Toronto
  • extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
  • focal adhesion Source: UniProtKB
  • growth cone Source: Alzheimers_University_of_Toronto
  • lamellipodium Source: UniProtKB
  • membrane raft Source: Ensembl
  • neuronal cell body Source: Alzheimers_University_of_Toronto
  • NMDA selective glutamate receptor complex Source: Alzheimers_University_of_Toronto
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • postsynaptic density Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Aberrant PTK2B/PYK2 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. Elevated PTK2B/PYK2 expression is seen in gliomas, hepatocellular carcinoma, lung cancer and breast cancer.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi402 – 4021Y → F: Abolishes autophosphorylation. Abolishes interaction with SRC. 1 Publication
Mutagenesisi457 – 4571K → A: Abolishes kinase activity. 1 Publication
Mutagenesisi859 – 8591P → A: Loss of interaction with NPHP1. 1 Publication
Mutagenesisi881 – 8811Y → F: Loss of phosphorylation site. Strongly reduced interaction with GRB2. 1 Publication

Organism-specific databases

PharmGKBiPA33956.

Chemistry

ChEMBLiCHEMBL5469.
DrugBankiDB01097. Leflunomide.
GuidetoPHARMACOLOGYi2181.

Polymorphism and mutation databases

BioMutaiPTK2B.
DMDMi3183003.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10091009Protein-tyrosine kinase 2-betaPRO_0000088081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei361 – 3611PhosphoserineCombined sources
Modified residuei375 – 3751PhosphoserineCombined sources
Modified residuei399 – 3991PhosphoserineCombined sources
Modified residuei402 – 4021Phosphotyrosine; by autocatalysis7 Publications
Modified residuei579 – 5791Phosphotyrosine; by SRC, LYN and LCKBy similarity
Modified residuei580 – 5801Phosphotyrosine; by SRC, LYN and LCK2 Publications
Modified residuei722 – 7221PhosphotyrosineCombined sources
Modified residuei762 – 7621PhosphoserineCombined sources
Modified residuei765 – 7651PhosphothreonineCombined sources
Modified residuei819 – 8191PhosphotyrosineCombined sources
Modified residuei834 – 8341PhosphotyrosineCombined sources
Modified residuei834 – 8341Phosphotyrosine; in variant Thr-838Combined sources
Modified residuei839 – 8391PhosphoserineCombined sources
Modified residuei842 – 8421PhosphothreonineCombined sources
Modified residuei842 – 8421Phosphothreonine; in variant Thr-838Combined sources
Modified residuei849 – 8491PhosphotyrosineCombined sources
Modified residuei866 – 8661PhosphoserineCombined sources
Modified residuei881 – 8811Phosphotyrosine; by SRC1 Publication

Post-translational modificationi

Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.9 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14289.
MaxQBiQ14289.
PaxDbiQ14289.
PeptideAtlasiQ14289.
PRIDEiQ14289.
TopDownProteomicsiQ14289-2. [Q14289-2]

PTM databases

iPTMnetiQ14289.
PhosphoSiteiQ14289.

Expressioni

Tissue specificityi

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.2 Publications

Gene expression databases

BgeeiENSG00000120899.
CleanExiHS_PTK2B.
ExpressionAtlasiQ14289. baseline and differential.
GenevisibleiQ14289. HS.

Organism-specific databases

HPAiCAB003850.
HPA026091.
HPA026276.

Interactioni

Subunit structurei

Homodimer, or homooligomer. Interacts with SIRPA and SH2D3C. Interacts with ARHGAP10. Interacts with DLG4 (By similarity). Interacts with KCNA2 (By similarity). Interacts with NPHP1, ASAP1, ASAP2, ARHGAP26, SKAP2 and TGFB1I1. The Tyr-402 phosphorylated form interacts with SRC (via SH2 domain) and SRC family members. Forms a signaling complex with EPHA1, LCK and phosphatidylinositol 3-kinase; upon activation by EFNA1. Interacts with GRB2 (via SH2 domain). Interacts with P53/TP53 and MDM2. Interacts with MYLK. Interacts with BCAR1. Interacts with PDPK1. Interacts (hypophosphorylated) with PXN. Interacts with RB1CC1. Interacts with RHOU. Interacts with VAV1. Interacts with LPXN and PTPN12.By similarity21 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHOUQ7L0Q84EBI-298640,EBI-1638043
SRCP129313EBI-298640,EBI-621482

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108480. 60 interactions.
IntActiQ14289. 14 interactions.
MINTiMINT-1211326.
STRINGi9606.ENSP00000332816.

Chemistry

BindingDBiQ14289.

Structurei

Secondary structure

1
1009
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 373Combined sources
Beta strandi38 – 458Combined sources
Beta strandi48 – 503Combined sources
Helixi52 – 543Combined sources
Beta strandi55 – 628Combined sources
Helixi67 – 7610Combined sources
Turni77 – 804Combined sources
Helixi87 – 893Combined sources
Beta strandi90 – 9910Combined sources
Beta strandi102 – 1054Combined sources
Helixi111 – 1177Combined sources
Turni119 – 1213Combined sources
Helixi124 – 1263Combined sources
Beta strandi127 – 1337Combined sources
Turni138 – 1403Combined sources
Helixi141 – 1444Combined sources
Helixi148 – 16518Combined sources
Turni166 – 1694Combined sources
Helixi172 – 18615Combined sources
Turni187 – 1893Combined sources
Helixi194 – 1963Combined sources
Helixi198 – 20710Combined sources
Helixi210 – 2123Combined sources
Helixi216 – 2216Combined sources
Helixi224 – 23815Combined sources
Helixi243 – 25412Combined sources
Beta strandi263 – 27311Combined sources
Beta strandi277 – 2826Combined sources
Beta strandi285 – 2895Combined sources
Beta strandi297 – 3004Combined sources
Helixi302 – 3043Combined sources
Beta strandi310 – 3134Combined sources
Turni314 – 3163Combined sources
Beta strandi317 – 3226Combined sources
Beta strandi331 – 3366Combined sources
Helixi338 – 35518Combined sources
Beta strandi356 – 3605Combined sources
Helixi422 – 4243Combined sources
Beta strandi425 – 4339Combined sources
Beta strandi435 – 44511Combined sources
Beta strandi447 – 4493Combined sources
Beta strandi451 – 4588Combined sources
Beta strandi461 – 4633Combined sources
Helixi465 – 48117Combined sources
Beta strandi489 – 4935Combined sources
Beta strandi495 – 4973Combined sources
Beta strandi499 – 5035Combined sources
Helixi510 – 5178Combined sources
Turni518 – 5203Combined sources
Helixi523 – 54220Combined sources
Beta strandi546 – 5483Combined sources
Helixi552 – 5543Combined sources
Beta strandi555 – 5595Combined sources
Beta strandi562 – 5654Combined sources
Helixi570 – 5723Combined sources
Helixi589 – 5913Combined sources
Helixi594 – 5996Combined sources
Helixi604 – 61916Combined sources
Turni620 – 6223Combined sources
Turni625 – 6284Combined sources
Helixi631 – 6333Combined sources
Helixi634 – 6407Combined sources
Helixi652 – 66110Combined sources
Helixi666 – 6683Combined sources
Helixi672 – 69120Combined sources
Helixi879 – 89719Combined sources
Helixi898 – 9003Combined sources
Helixi903 – 9053Combined sources
Helixi906 – 92722Combined sources
Helixi928 – 9303Combined sources
Helixi933 – 96230Combined sources
Turni963 – 9653Combined sources
Helixi969 – 100436Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FO6model-X45-350[»]
2LK4NMR-A871-1005[»]
3CC6X-ray1.60A414-692[»]
3ET7X-ray2.70A416-692[»]
3FZOX-ray2.20A416-692[»]
3FZPX-ray2.10A416-692[»]
3FZRX-ray2.70A416-692[»]
3FZSX-ray1.75A416-692[»]
3FZTX-ray1.95A416-692[»]
3GM1X-ray2.95A/B861-1009[»]
3GM2X-ray2.71A861-1009[»]
3GM3X-ray2.60A861-1009[»]
3H3CX-ray2.00A416-692[»]
3U3FX-ray3.10A/B/C/D871-1005[»]
4EKUX-ray3.25A21-409[»]
4H1JX-ray2.00A416-692[»]
4H1MX-ray1.99A416-692[»]
4R32X-ray3.50A871-1005[»]
4XEFX-ray2.50A/D871-1005[»]
4XEKX-ray1.79A871-1005[»]
4XEVX-ray2.01A/D871-1005[»]
ProteinModelPortaliQ14289.
SMRiQ14289. Positions 21-366, 415-692, 867-1007.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 359321FERMPROSITE-ProRule annotationAdd
BLAST
Domaini425 – 683259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni801 – 1009209Interaction with TGFB1I1By similarityAdd
BLAST
Regioni868 – 1009142Focal adhesion targeting (FAT)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi702 – 76766Pro-richAdd
BLAST
Compositional biasi831 – 86939Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4257. Eukaryota.
ENOG410ZH9Y. LUCA.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000069938.
HOVERGENiHBG004018.
InParanoidiQ14289.
KOiK05871.
OMAiVKTCKKD.
OrthoDBiEOG091G03BN.
PhylomeDBiQ14289.
TreeFamiTF316643.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR030610. PTK2B.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR24418:SF94. PTHR24418:SF94. 1 hit.
PfamiPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14289-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGVSEPLSR VKLGTLRRPE GPAEPMVVVP VDVEKEDVRI LKVCFYSNSF
60 70 80 90 100
NPGKNFKLVK CTVQTEIREI ITSILLSGRI GPNIRLAECY GLRLKHMKSD
110 120 130 140 150
EIHWLHPQMT VGEVQDKYEC LHVEAEWRYD LQIRYLPEDF MESLKEDRTT
160 170 180 190 200
LLYFYQQLRN DYMQRYASKV SEGMALQLGC LELRRFFKDM PHNALDKKSN
210 220 230 240 250
FELLEKEVGL DLFFPKQMQE NLKPKQFRKM IQQTFQQYAS LREEECVMKF
260 270 280 290 300
FNTLAGFANI DQETYRCELI QGWNITVDLV IGPKGIRQLT SQDAKPTCLA
310 320 330 340 350
EFKQIRSIRC LPLEEGQAVL QLGIEGAPQA LSIKTSSLAE AENMADLIDG
360 370 380 390 400
YCRLQGEHQG SLIIHPRKDG EKRNSLPQIP MLNLEARRSH LSESCSIESD
410 420 430 440 450
IYAEIPDETL RRPGGPQYGI AREDVVLNRI LGEGFFGEVY EGVYTNHKGE
460 470 480 490 500
KINVAVKTCK KDCTLDNKEK FMSEAVIMKN LDHPHIVKLI GIIEEEPTWI
510 520 530 540 550
IMELYPYGEL GHYLERNKNS LKVLTLVLYS LQICKAMAYL ESINCVHRDI
560 570 580 590 600
AVRNILVASP ECVKLGDFGL SRYIEDEDYY KASVTRLPIK WMSPESINFR
610 620 630 640 650
RFTTASDVWM FAVCMWEILS FGKQPFFWLE NKDVIGVLEK GDRLPKPDLC
660 670 680 690 700
PPVLYTLMTR CWDYDPSDRP RFTELVCSLS DVYQMEKDIA MEQERNARYR
710 720 730 740 750
TPKILEPTAF QEPPPKPSRP KYRPPPQTNL LAPKLQFQVP EGLCASSPTL
760 770 780 790 800
TSPMEYPSPV NSLHTPPLHR HNVFKRHSMR EEDFIQPSSR EEAQQLWEAE
810 820 830 840 850
KVKMRQILDK QQKQMVEDYQ WLRQEEKSLD PMVYMNDKSP LTPEKEVGYL
860 870 880 890 900
EFTGPPQKPP RLGAQSIQPT ANLDRTDDLV YLNVMELVRA VLELKNELCQ
910 920 930 940 950
LPPEGYVVVV KNVGLTLRKL IGSVDDLLPS LPSSSRTEIE GTQKLLNKDL
960 970 980 990 1000
AELINKMRLA QQNAVTSLSE ECKRQMLTAS HTLAVDAKNL LDAVDQAKVL

ANLAHPPAE
Length:1,009
Mass (Da):115,875
Last modified:July 15, 1998 - v2
Checksum:i420B21046274E7C2
GO
Isoform 2 (identifier: Q14289-2) [UniParc]FASTAAdd to basket
Also known as: PYK2H

The sequence of this isoform differs from the canonical sequence as follows:
     739-780: Missing.

Show »
Length:967
Mass (Da):111,183
Checksum:i4AFDAA83908F2902
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231A → G in BAA08289 (PubMed:7673154).Curated
Sequence conflicti23 – 231A → G in AAC05330 (PubMed:7673154).Curated
Sequence conflicti256 – 2561G → P in AAB47217 (PubMed:8838818).Curated
Sequence conflicti435 – 4351F → L in AAC05330 (PubMed:7673154).Curated
Sequence conflicti780 – 7801R → G in AAB47217 (PubMed:8838818).Curated
Sequence conflicti985 – 9851V → M in AAH36651 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti359 – 3591Q → E.1 Publication
Corresponds to variant rs56175011 [ dbSNP | Ensembl ].
VAR_041687
Natural varianti698 – 6981R → H.1 Publication
Corresponds to variant rs35174236 [ dbSNP | Ensembl ].
VAR_041688
Natural varianti808 – 8081L → P.1 Publication
Corresponds to variant rs55747955 [ dbSNP | Ensembl ].
VAR_041689
Natural varianti838 – 8381K → T.Combined sources1 Publication
Corresponds to variant rs751019 [ dbSNP | Ensembl ].
VAR_020284
Natural varianti970 – 9701E → K.1 Publication
Corresponds to variant rs56263944 [ dbSNP | Ensembl ].
VAR_041690

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei739 – 78042Missing in isoform 2. 1 PublicationVSP_004981Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33284 mRNA. Translation: AAC50203.1.
L49207 mRNA. Translation: AAB47217.1.
D45853 mRNA. Translation: BAA08289.1.
U43522 mRNA. Translation: AAC05330.1.
S80542 mRNA. Translation: AAB35701.1.
AF311103 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63553.1.
CH471080 Genomic DNA. Translation: EAW63555.1.
CH471080 Genomic DNA. Translation: EAW63556.1.
BC036651 mRNA. Translation: AAH36651.1.
BC042599 mRNA. Translation: AAH42599.1.
CCDSiCCDS6057.1. [Q14289-1]
CCDS6058.1. [Q14289-2]
PIRiS60248.
RefSeqiNP_004094.3. NM_004103.4. [Q14289-1]
NP_775266.1. NM_173174.2. [Q14289-1]
NP_775267.1. NM_173175.2. [Q14289-2]
NP_775268.1. NM_173176.2. [Q14289-1]
XP_005273504.1. XM_005273447.4. [Q14289-1]
XP_011542743.1. XM_011544441.2. [Q14289-1]
UniGeneiHs.491322.
Hs.735450.

Genome annotation databases

EnsembliENST00000346049; ENSP00000332816; ENSG00000120899. [Q14289-1]
ENST00000397501; ENSP00000380638; ENSG00000120899. [Q14289-1]
ENST00000420218; ENSP00000391995; ENSG00000120899. [Q14289-2]
ENST00000517339; ENSP00000427931; ENSG00000120899. [Q14289-2]
GeneIDi2185.
KEGGihsa:2185.
UCSCiuc003xfn.3. human. [Q14289-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33284 mRNA. Translation: AAC50203.1.
L49207 mRNA. Translation: AAB47217.1.
D45853 mRNA. Translation: BAA08289.1.
U43522 mRNA. Translation: AAC05330.1.
S80542 mRNA. Translation: AAB35701.1.
AF311103 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63553.1.
CH471080 Genomic DNA. Translation: EAW63555.1.
CH471080 Genomic DNA. Translation: EAW63556.1.
BC036651 mRNA. Translation: AAH36651.1.
BC042599 mRNA. Translation: AAH42599.1.
CCDSiCCDS6057.1. [Q14289-1]
CCDS6058.1. [Q14289-2]
PIRiS60248.
RefSeqiNP_004094.3. NM_004103.4. [Q14289-1]
NP_775266.1. NM_173174.2. [Q14289-1]
NP_775267.1. NM_173175.2. [Q14289-2]
NP_775268.1. NM_173176.2. [Q14289-1]
XP_005273504.1. XM_005273447.4. [Q14289-1]
XP_011542743.1. XM_011544441.2. [Q14289-1]
UniGeneiHs.491322.
Hs.735450.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FO6model-X45-350[»]
2LK4NMR-A871-1005[»]
3CC6X-ray1.60A414-692[»]
3ET7X-ray2.70A416-692[»]
3FZOX-ray2.20A416-692[»]
3FZPX-ray2.10A416-692[»]
3FZRX-ray2.70A416-692[»]
3FZSX-ray1.75A416-692[»]
3FZTX-ray1.95A416-692[»]
3GM1X-ray2.95A/B861-1009[»]
3GM2X-ray2.71A861-1009[»]
3GM3X-ray2.60A861-1009[»]
3H3CX-ray2.00A416-692[»]
3U3FX-ray3.10A/B/C/D871-1005[»]
4EKUX-ray3.25A21-409[»]
4H1JX-ray2.00A416-692[»]
4H1MX-ray1.99A416-692[»]
4R32X-ray3.50A871-1005[»]
4XEFX-ray2.50A/D871-1005[»]
4XEKX-ray1.79A871-1005[»]
4XEVX-ray2.01A/D871-1005[»]
ProteinModelPortaliQ14289.
SMRiQ14289. Positions 21-366, 415-692, 867-1007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108480. 60 interactions.
IntActiQ14289. 14 interactions.
MINTiMINT-1211326.
STRINGi9606.ENSP00000332816.

Chemistry

BindingDBiQ14289.
ChEMBLiCHEMBL5469.
DrugBankiDB01097. Leflunomide.
GuidetoPHARMACOLOGYi2181.

PTM databases

iPTMnetiQ14289.
PhosphoSiteiQ14289.

Polymorphism and mutation databases

BioMutaiPTK2B.
DMDMi3183003.

Proteomic databases

EPDiQ14289.
MaxQBiQ14289.
PaxDbiQ14289.
PeptideAtlasiQ14289.
PRIDEiQ14289.
TopDownProteomicsiQ14289-2. [Q14289-2]

Protocols and materials databases

DNASUi2185.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346049; ENSP00000332816; ENSG00000120899. [Q14289-1]
ENST00000397501; ENSP00000380638; ENSG00000120899. [Q14289-1]
ENST00000420218; ENSP00000391995; ENSG00000120899. [Q14289-2]
ENST00000517339; ENSP00000427931; ENSG00000120899. [Q14289-2]
GeneIDi2185.
KEGGihsa:2185.
UCSCiuc003xfn.3. human. [Q14289-1]

Organism-specific databases

CTDi2185.
GeneCardsiPTK2B.
H-InvDBHIX0168898.
HGNCiHGNC:9612. PTK2B.
HPAiCAB003850.
HPA026091.
HPA026276.
MIMi601212. gene.
neXtProtiNX_Q14289.
PharmGKBiPA33956.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4257. Eukaryota.
ENOG410ZH9Y. LUCA.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000069938.
HOVERGENiHBG004018.
InParanoidiQ14289.
KOiK05871.
OMAiVKTCKKD.
OrthoDBiEOG091G03BN.
PhylomeDBiQ14289.
TreeFamiTF316643.

Enzyme and pathway databases

BRENDAi2.7.10.2. 2681.
ReactomeiR-HSA-391160. Signal regulatory protein (SIRP) family interactions.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-451927. Interleukin-2 signaling.
SignaLinkiQ14289.
SIGNORiQ14289.

Miscellaneous databases

ChiTaRSiPTK2B. human.
EvolutionaryTraceiQ14289.
GeneWikiiPTK2B.
GenomeRNAii2185.
PROiQ14289.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120899.
CleanExiHS_PTK2B.
ExpressionAtlasiQ14289. baseline and differential.
GenevisibleiQ14289. HS.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR030610. PTK2B.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR24418:SF94. PTHR24418:SF94. 1 hit.
PfamiPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFAK2_HUMAN
AccessioniPrimary (citable) accession number: Q14289
Secondary accession number(s): D3DST0
, Q13475, Q14290, Q16709, Q6PID4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 192 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Promotes bone resorption, and thus PTK2B/PYK2 inhibitors might be used to treat osteoporosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.