Q14257 (RCN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulocalbin-2

Alternative name(s):
Calcium-binding protein ERC-55
E6-binding protein
Short name=E6BP

Gene names

Name:	RCN2
Synonyms:	ERC55

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known. Binds calcium.
Subcellular location	Endoplasmic reticulum lumen.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the CREC family. Contains 6 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Traceable author statement Ref.1. Source: ProtInc endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 317	295	Reticulocalbin-2		PRO_0000004148
Regions
	Domain	61 – 96	36	EF-hand 1
	Domain	97 – 132	36	EF-hand 2
	Domain	150 – 185	36	EF-hand 3
	Domain	186 – 221	36	EF-hand 4
	Domain	227 – 262	36	EF-hand 5
	Domain	263 – 298	36	EF-hand 6
	Calcium binding	74 – 85	12	1 Potential
	Calcium binding	110 – 121	12	2 Potential
	Calcium binding	162 – 173	12	3; possibly ancestral Potential
	Calcium binding	199 – 210	12	4 Potential
	Calcium binding	240 – 251	12	5 Potential
	Calcium binding	276 – 287	12	6 Potential
	Motif	314 – 317	4	Prevents secretion from ER Potential

Sequences

	Sequence		Length	Mass (Da)
	Q14257 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E7C0A4211DBF04AB	FASTA	317	36,876
	10 20 30 40 50 60 MRLGPRTAAL GLLLLCAAAA GAGKAEELHY PLGERRSDYD REALLGVQED VDEYVKLGHE 70 80 90 100 110 120 EQQKRLQAII KKIDLDSDGF LTESELSSWI QMSFKHYAMQ EAKQQFVEYD KNSDDTVTWD 130 140 150 160 170 180 EYNIQMYDRV IDFDENTALD DAEEESFRKL HLKDKKRFEK ANQDSGPGLS LEEFIAFEHP 190 200 210 220 230 240 EEVDYMTEFV IQEALEEHDK NGDGFVSLEE FLGDYRWDPT ANEDPEWILV EKDRFVNDYD 250 260 270 280 290 300 KDNDGRLDPQ ELLPWVVPNN QGIAQEEALH LIDEMDLNGD KKLSEEEILE NPDLFLTSEA 310 TDYGRQLHDD YFYHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The endoplasmic reticulum calcium-binding protein of 55 kDa is a novel EF-hand protein retained in the endoplasmic reticulum by a carboxyl-terminal His-Asp-Glu-Leu motif." Weis K., Griffiths G., Lamond A.I. J. Biol. Chem. 269:19142-19150(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	Bienvenut W.V., Vousden K.H., Lukashchuk N. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 42-56; 130-149 AND 283-305, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78669 mRNA. Translation: CAA55343.1. BT009790 mRNA. Translation: AAP88792.1. AK313833 mRNA. Translation: BAG36566.1. AC051643 Genomic DNA. No translation available. BC004892 mRNA. Translation: AAH04892.1.
IPI	IPI00029628.
PIR	I37371.
RefSeq	NP_001258766.1. NM_001271837.1. NP_002893.1. NM_002902.2.
UniGene	Hs.79088.
3D structure databases
ProteinModelPortal	Q14257.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14257. 14 interactions.
MINT	MINT-1146851.
STRING	9606.ENSP00000378349.
PTM databases
PhosphoSite	Q14257.
Polymorphism databases
DMDM	2493460.
Proteomic databases
PaxDb	Q14257.
PRIDE	Q14257.
Protocols and materials databases
DNASU	5955.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000394885; ENSP00000378349; ENSG00000117906.
GeneID	5955.
KEGG	hsa:5955.
UCSC	uc002bcd.3. human.
Organism-specific databases
CTD	5955.
GeneCards	GC15P077223.
HGNC	HGNC:9935. RCN2.
MIM	602584. gene.
neXtProt	NX_Q14257.
PharmGKB	PA34304.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG124414.
HOGENOM	HOG000230934.
HOVERGEN	HBG002834.
OrthoDB	EOG4DFPP6.
PhylomeDB	Q14257.
Gene expression databases
ArrayExpress	Q14257.
Bgee	Q14257.
CleanEx	HS_RCN2.
Genevestigator	Q14257.
GermOnline	ENSG00000117906. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13202. EF_hand_3. 1 hit. PF13499. EF_hand_5. 2 hits. [Graphical view]
SMART	SM00054. EFh. 5 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 5 hits. PS50222. EF_HAND_2. 5 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5955.
NextBio	23192.
SOURCE	Search...

Entry information

Entry name

RCN2_HUMAN

Accession

Primary (citable) accession number: Q14257
Secondary accession number(s): A8MTG6, Q53XN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.