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Protein

Reticulocalbin-2

Gene

RCN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Not known. Binds calcium.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi74 – 85121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi110 – 121122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi162 – 173123; possibly ancestralPROSITE-ProRule annotationAdd
BLAST
Calcium bindingi199 – 210124PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi240 – 251125PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi276 – 287126PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Reticulocalbin-2
Alternative name(s):
Calcium-binding protein ERC-55
E6-binding protein
Short name:
E6BP
Gene namesi
Name:RCN2
Synonyms:ERC55
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:9935. RCN2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: HPA
  2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. nucleolus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34304.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 317295Reticulocalbin-2PRO_0000004148Add
BLAST

Proteomic databases

MaxQBiQ14257.
PaxDbiQ14257.
PRIDEiQ14257.

PTM databases

PhosphoSiteiQ14257.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ14257.
CleanExiHS_RCN2.
ExpressionAtlasiQ14257. baseline and differential.
GenevestigatoriQ14257.

Organism-specific databases

HPAiHPA030694.
HPA030695.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
E6P031262EBI-356710,EBI-1177242From a different organism.
GABARAPL2P605203EBI-356710,EBI-720116

Protein-protein interaction databases

BioGridi111888. 38 interactions.
IntActiQ14257. 29 interactions.
MINTiMINT-1146851.
STRINGi9606.ENSP00000378349.

Structurei

3D structure databases

ProteinModelPortaliQ14257.
SMRiQ14257. Positions 52-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 9636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 13236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini150 – 18536EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini186 – 22136EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini227 – 26236EF-hand 5PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 29836EF-hand 6PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi314 – 3174Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the CREC family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG124414.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000230934.
HOVERGENiHBG002834.
InParanoidiQ14257.
OMAiRFEKANR.
OrthoDBiEOG73Z2TD.
PhylomeDBiQ14257.
TreeFamiTF314849.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14257-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLGPRTAAL GLLLLCAAAA GAGKAEELHY PLGERRSDYD REALLGVQED
60 70 80 90 100
VDEYVKLGHE EQQKRLQAII KKIDLDSDGF LTESELSSWI QMSFKHYAMQ
110 120 130 140 150
EAKQQFVEYD KNSDDTVTWD EYNIQMYDRV IDFDENTALD DAEEESFRKL
160 170 180 190 200
HLKDKKRFEK ANQDSGPGLS LEEFIAFEHP EEVDYMTEFV IQEALEEHDK
210 220 230 240 250
NGDGFVSLEE FLGDYRWDPT ANEDPEWILV EKDRFVNDYD KDNDGRLDPQ
260 270 280 290 300
ELLPWVVPNN QGIAQEEALH LIDEMDLNGD KKLSEEEILE NPDLFLTSEA
310
TDYGRQLHDD YFYHDEL
Length:317
Mass (Da):36,876
Last modified:October 31, 1997 - v1
Checksum:iE7C0A4211DBF04AB
GO
Isoform 2 (identifier: Q14257-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-149: K → KEFAICKKQSFCFWLLRFN

Note: Gene prediction based on EST data.

Show »
Length:335
Mass (Da):39,139
Checksum:i78B9EE873599B45C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 1491K → KEFAICKKQSFCFWLLRFN in isoform 2. CuratedVSP_055139

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78669 mRNA. Translation: CAA55343.1.
BT009790 mRNA. Translation: AAP88792.1.
AK313833 mRNA. Translation: BAG36566.1.
AC051643 Genomic DNA. No translation available.
BC004892 mRNA. Translation: AAH04892.1.
CCDSiCCDS10291.1. [Q14257-1]
CCDS61719.1. [Q14257-2]
PIRiI37371.
RefSeqiNP_001258766.1. NM_001271837.1. [Q14257-2]
NP_002893.1. NM_002902.2. [Q14257-1]
UniGeneiHs.79088.

Genome annotation databases

EnsembliENST00000320963; ENSP00000319739; ENSG00000117906. [Q14257-2]
ENST00000394885; ENSP00000378349; ENSG00000117906. [Q14257-1]
GeneIDi5955.
KEGGihsa:5955.
UCSCiuc002bcd.3. human. [Q14257-1]

Polymorphism databases

DMDMi2493460.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78669 mRNA. Translation: CAA55343.1.
BT009790 mRNA. Translation: AAP88792.1.
AK313833 mRNA. Translation: BAG36566.1.
AC051643 Genomic DNA. No translation available.
BC004892 mRNA. Translation: AAH04892.1.
CCDSiCCDS10291.1. [Q14257-1]
CCDS61719.1. [Q14257-2]
PIRiI37371.
RefSeqiNP_001258766.1. NM_001271837.1. [Q14257-2]
NP_002893.1. NM_002902.2. [Q14257-1]
UniGeneiHs.79088.

3D structure databases

ProteinModelPortaliQ14257.
SMRiQ14257. Positions 52-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111888. 38 interactions.
IntActiQ14257. 29 interactions.
MINTiMINT-1146851.
STRINGi9606.ENSP00000378349.

PTM databases

PhosphoSiteiQ14257.

Polymorphism databases

DMDMi2493460.

Proteomic databases

MaxQBiQ14257.
PaxDbiQ14257.
PRIDEiQ14257.

Protocols and materials databases

DNASUi5955.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000320963; ENSP00000319739; ENSG00000117906. [Q14257-2]
ENST00000394885; ENSP00000378349; ENSG00000117906. [Q14257-1]
GeneIDi5955.
KEGGihsa:5955.
UCSCiuc002bcd.3. human. [Q14257-1]

Organism-specific databases

CTDi5955.
GeneCardsiGC15P077223.
HGNCiHGNC:9935. RCN2.
HPAiHPA030694.
HPA030695.
MIMi602584. gene.
neXtProtiNX_Q14257.
PharmGKBiPA34304.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG124414.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000230934.
HOVERGENiHBG002834.
InParanoidiQ14257.
OMAiRFEKANR.
OrthoDBiEOG73Z2TD.
PhylomeDBiQ14257.
TreeFamiTF314849.

Miscellaneous databases

ChiTaRSiRCN2. human.
GeneWikiiRCN2.
GenomeRNAii5955.
NextBioi23192.
PROiQ14257.
SOURCEiSearch...

Gene expression databases

BgeeiQ14257.
CleanExiHS_RCN2.
ExpressionAtlasiQ14257. baseline and differential.
GenevestigatoriQ14257.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The endoplasmic reticulum calcium-binding protein of 55 kDa is a novel EF-hand protein retained in the endoplasmic reticulum by a carboxyl-terminal His-Asp-Glu-Leu motif."
    Weis K., Griffiths G., Lamond A.I.
    J. Biol. Chem. 269:19142-19150(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  6. Bienvenut W.V., Vousden K.H., Lukashchuk N.
    Submitted (FEB-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 42-56; 130-149 AND 283-305, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Lung carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRCN2_HUMAN
AccessioniPrimary (citable) accession number: Q14257
Secondary accession number(s): A8MTG6, F8WCY5, Q53XN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1997
Last modified: March 3, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.