Reviewed, UniProtKB/Swiss-Prot Q14254 (FLOT2_HUMAN)

Names and origin

Protein names

Recommended name:
    Flotillin-2
Alternative name(s):
    Epidermal surface antigen
      Short name=ESA
    Membrane component chromosome 17 surface marker 1

Gene names

Name:	FLOT2
Synonyms:	ESA1, M17S1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Subunit structure	Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Note: Membrane-associated protein of caveolae.
Tissue specificity	In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Sequence similarities	Belongs to the band 7/mec-2 family. Flotillin subfamily.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell adhesion Ref.2 Non-traceable author statement. Source: ProtInc epidermis development Ref.2 Traceable author statement. Source: ProtInc
Cellular component	cell surface Inferred from direct assay. Source: UniProtKB extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: UniProtKB plasma membrane Non-traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAV1	Q03135	1	EBI-348613,EBI-603614
DOM3Z	O77932	1	EBI-348613,EBI-372173

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Flotillin-2		PRO_0000094049
Amino acid modifications
	Modified residue	241	1	Phosphotyrosine Ref.6
Natural variations
	Natural variant	328	1	A → T: dbSNP rs3736238.		VAR_024375
Experimental info
	Sequence conflict	8	1	G → R in AAA65729. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q14254-1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: F690BFE0151D3BD7	FASTA	428	47,064
	10 20 30 40 50 60 MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM TLQPRCEDVE 70 80 90 100 110 120 TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK NVVLQTLEGH LRSILGTLTV 130 140 150 160 170 180 EQIYQDRDQF AKLVREVAAP DVGRMGIEIL SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD 190 200 210 220 230 240 IGVAEAERDA GIREAECKKE MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA 250 260 270 280 290 300 YELQGAREQQ KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI 310 320 330 340 350 360 AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK YGDAAKMALV 370 380 390 400 410 420 LEALPQIAAK IAAPLTKVDE IVVLSGDNSK VTSEVNRLLA ELPASVHALT GVDLSKIPLI KKATGVQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Cloning and characterization of a novel epidermal cell surface antigen (ESA)." Schroeder W.T., Stewart-Galetka S., Mandavilli S., Parry D.A., Goldsmith L., Duvic M. J. Biol. Chem. 269:19983-19991(1994) [PubMed: 8051082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-428. Tissue: Keratinocyte.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-428. Tissue: Pancreas.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-428.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-241, MASS SPECTROMETRY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC024267 Genomic DNA. No translation available. M60922 mRNA. Translation: AAA65729.1. Different initiation. BC017292 mRNA. Translation: AAH17292.2. BT019478 mRNA. Translation: AAV38285.1. Different initiation.
IPI	IPI00789008.
PIR	A53664.
RefSeq	NP_004466.2.
UniGene	Hs.514038
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14254. 4 interactions.
STRING	Q14254.
PTM databases
PhosphoSite	Q14254.
Proteomic databases
PRIDE	Q14254.
Genome annotation databases
Ensembl	ENST00000394908; ENSP00000378368; ENSG00000132589; Homo sapiens. [Genome view]
GeneID	2319.
KEGG	hsa:2319.
Organism-specific databases
CTD	2319.
GeneCards	GC17M024230.
H-InvDB	HIX0013665.
HGNC	HGNC:3758. FLOT2.
HPA	CAB004395. HPA001396.
MIM	131560. gene.
PharmGKB	PA28176.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18558.
HOGENOM	HBG729052.
HOVERGEN	Q14254.
InParanoid	Q14254.
OrthoDB	EOG92FW35.
PhylomeDB	Q14254.
Gene expression databases
ArrayExpress	Q14254.
Bgee	Q14254.
CleanEx	HS_FLOT2.
Genevestigator	Q14254.
GermOnline	ENSG00000132589. Homo sapiens.
Family and domain databases
InterPro	IPR001107. Band_7. [Graphical view]
Pfam	PF01145. Band_7. 1 hit. [Graphical view]
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

FLOT2_HUMAN

Accession

Primary (citable) accession number: Q14254

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 28, 2009
Last modified:	February 9, 2010
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.