Q14254 (FLOT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flotillin-2

Alternative name(s):
Epidermal surface antigen
Short name=ESA
Membrane component chromosome 17 surface marker 1

Gene names

Name:	FLOT2
Synonyms:	ESA1, M17S1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Subunit structure	Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 By similarity. Interacts with ECM29. Ref.7
Subcellular location	Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Endosome. Note: Membrane-associated protein of caveolae. Ref.7
Tissue specificity	In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Sequence similarities	Belongs to the band 7/mec-2 family. Flotillin subfamily.
Sequence caution	The sequence AAA65729.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Endosome Membrane
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Non-traceable author statement. Source: ProtInc epidermis development Traceable author statement. Source: ProtInc
Cellular component	cell surface Inferred from direct assay. Source: UniProtKB endocytic vesicle Inferred from direct assay Ref.7. Source: UniProtKB endosome Inferred from direct assay Ref.7. Source: UniProtKB membrane fraction Inferred from direct assay. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Flotillin-2		PRO_0000094049
Amino acid modifications
	Modified residue	241	1	Phosphotyrosine Ref.5 Ref.6
Natural variations
	Natural variant	328	1	A → T. Corresponds to variant rs3736238 [ dbSNP | Ensembl ].		VAR_024375
Experimental info
	Sequence conflict	8	1	G → R in AC024267. Ref.1
	Sequence conflict	8	1	G → R in AAA65729. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q14254 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: F690BFE0151D3BD7	FASTA	428	47,064
	10 20 30 40 50 60 MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM TLQPRCEDVE 70 80 90 100 110 120 TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK NVVLQTLEGH LRSILGTLTV 130 140 150 160 170 180 EQIYQDRDQF AKLVREVAAP DVGRMGIEIL SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD 190 200 210 220 230 240 IGVAEAERDA GIREAECKKE MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA 250 260 270 280 290 300 YELQGAREQQ KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI 310 320 330 340 350 360 AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK YGDAAKMALV 370 380 390 400 410 420 LEALPQIAAK IAAPLTKVDE IVVLSGDNSK VTSEVNRLLA ELPASVHALT GVDLSKIPLI KKATGVQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Cloning and characterization of a novel epidermal cell surface antigen (ESA)." Schroeder W.T., Stewart-Galetka S., Mandavilli S., Parry D.A., Goldsmith L., Duvic M. J. Biol. Chem. 269:19983-19991(1994) [PubMed: 8051082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-428. Tissue: Keratinocyte.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-428. Tissue: Pancreas.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-428.
[5]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-241, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[6]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-241, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[7]	"A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components." Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M. J. Biol. Chem. 285:31616-31633(2010) [PubMed: 20682791] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ECM29.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC024267 Genomic DNA. No translation available. M60922 mRNA. Translation: AAA65729.1. Different initiation. BC017292 mRNA. Translation: AAH17292.2. BT019478 mRNA. Translation: AAV38285.1.
IPI	IPI00789008.
PIR	A53664.
RefSeq	NP_004466.2. NM_004475.2.
UniGene	Hs.514038.
3D structure databases
ProteinModelPortal	Q14254.
SMR	Q14254. Positions 40-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14254. 9 interactions.
MINT	MINT-1034434.
STRING	Q14254.
PTM databases
PhosphoSite	Q14254.
Polymorphism databases
DMDM	254763294.
Proteomic databases
PRIDE	Q14254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000394908; ENSP00000378368; ENSG00000132589.
GeneID	2319.
KEGG	hsa:2319.
Organism-specific databases
CTD	2319.
GeneCards	GC17M027206.
H-InvDB	HIX0013665.
HGNC	HGNC:3758. FLOT2.
HPA	CAB004395. HPA001396.
MIM	131560. gene.
neXtProt	NX_Q14254.
PharmGKB	PA28176.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18558.
HOGENOM	HBG729052.
HOVERGEN	HBG051628.
InParanoid	Q14254.
Gene expression databases
ArrayExpress	Q14254.
Bgee	Q14254.
CleanEx	HS_FLOT2.
Genevestigator	Q14254.
GermOnline	ENSG00000132589. Homo sapiens.
Family and domain databases
InterPro	IPR001107. Band_7. [Graphical view]
KO	K07192.
Pfam	PF01145. Band_7. 1 hit. [Graphical view]
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

FLOT2_HUMAN

Accession

Primary (citable) accession number: Q14254

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 28, 2009
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.