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Protein

Flotillin-2

Gene

FLOT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: ProtInc
  • epidermis development Source: ProtInc
  • establishment of protein localization to plasma membrane Source: UniProtKB
  • membrane raft assembly Source: UniProtKB
  • negative regulation of amyloid precursor protein catabolic process Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • positive regulation of establishment of T cell polarity Source: UniProtKB
  • protein localization to plasma membrane raft Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • regulation of myoblast differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Flotillin-2
Alternative name(s):
Epidermal surface antigen
Short name:
ESA
Membrane component chromosome 17 surface marker 1
Gene namesi
Name:FLOT2
Synonyms:ESA1, M17S1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3758. FLOT2.

Subcellular locationi

GO - Cellular componenti

  • acrosomal membrane Source: Ensembl
  • basolateral plasma membrane Source: UniProtKB
  • caveola Source: MGI
  • cell-cell adherens junction Source: UniProtKB
  • cell-cell contact zone Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB
  • endocytic vesicle Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • flotillin complex Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • membrane Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • uropod Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41C → S: Loss of ZDHHC5-catalyzed palmitoylation; when associated with S-20. Partial loss of ZDHHC5-catalyzed palmitoylation; when associated with S-19. Complete loss of palmitoylation; when associated with S-19 and S-20. 1 Publication
Mutagenesisi19 – 191C → S: Partial loss of ZDHHC5-catalyzed palmitoylation; when associated with S-4 or S-20. Complete loss of palmitoylation; when associated with S-4 and S-20. 1 Publication
Mutagenesisi20 – 201C → S: Loss of ZDHHC5-catalyzed palmitoylation; when associated with S-4. Partial loss of ZDHHC5-catalyzed palmitoylation; when associated with S-19. Complete loss of palmitoylation; when associated with S-4 and S-19. 1 Publication

Organism-specific databases

PharmGKBiPA28176.

Polymorphism and mutation databases

BioMutaiFLOT2.
DMDMi254763294.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 428427Flotillin-2PRO_0000094049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Lipidationi4 – 41S-palmitoyl cysteine; by ZDHHC51 Publication
Lipidationi19 – 191S-palmitoyl cysteine1 Publication
Lipidationi20 – 201S-palmitoyl cysteine; by ZDHHC51 Publication
Modified residuei405 – 4051PhosphoserineCombined sources

Post-translational modificationi

ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ14254.
MaxQBiQ14254.
PaxDbiQ14254.
PRIDEiQ14254.

PTM databases

iPTMnetiQ14254.
PhosphoSiteiQ14254.
SwissPalmiQ14254.

Expressioni

Tissue specificityi

In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.

Gene expression databases

BgeeiQ14254.
CleanExiHS_FLOT2.
ExpressionAtlasiQ14254. baseline and differential.
GenevisibleiQ14254. HS.

Organism-specific databases

HPAiCAB004395.
HPA001396.

Interactioni

Subunit structurei

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECM29.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BACE1P568172EBI-348613,EBI-2433139
FLOT1O759555EBI-348613,EBI-603643
SNCGQ6FHG52EBI-348613,EBI-9776333

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi108608. 85 interactions.
IntActiQ14254. 61 interactions.
MINTiMINT-1034434.
STRINGi9606.ENSP00000378368.

Structurei

3D structure databases

ProteinModelPortaliQ14254.
SMRiQ14254. Positions 40-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ14254.
KOiK07192.
PhylomeDBiQ14254.
TreeFamiTF324879.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q14254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM
60 70 80 90 100
TLQPRCEDVE TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK
110 120 130 140 150
NVVLQTLEGH LRSILGTLTV EQIYQDRDQF AKLVREVAAP DVGRMGIEIL
160 170 180 190 200
SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD IGVAEAERDA GIREAECKKE
210 220 230 240 250
MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA YELQGAREQQ
260 270 280 290 300
KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI
310 320 330 340 350
AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK
360 370 380 390 400
YGDAAKMALV LEALPQIAAK IAAPLTKVDE IVVLSGDNSK VTSEVNRLLA
410 420
ELPASVHALT GVDLSKIPLI KKATGVQV
Length:428
Mass (Da):47,064
Last modified:July 28, 2009 - v2
Checksum:iF690BFE0151D3BD7
GO

Sequence cautioni

The sequence AAA65729.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81G → R in AC024267 (PubMed:16625196).Curated
Sequence conflicti8 – 81G → R in AAA65729 (PubMed:8051082).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti328 – 3281A → T.
Corresponds to variant rs3736238 [ dbSNP | Ensembl ].
VAR_024375

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024267 Genomic DNA. No translation available.
M60922 mRNA. Translation: AAA65729.1. Different initiation.
BC017292 mRNA. Translation: AAH17292.2.
BT019478 mRNA. Translation: AAV38285.1.
CCDSiCCDS11245.2.
PIRiA53664.
RefSeqiNP_004466.2. NM_004475.2.
XP_005258009.1. XM_005257952.1.
UniGeneiHs.514038.

Genome annotation databases

EnsembliENST00000394908; ENSP00000378368; ENSG00000132589.
GeneIDi2319.
KEGGihsa:2319.
UCSCiuc002hdc.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024267 Genomic DNA. No translation available.
M60922 mRNA. Translation: AAA65729.1. Different initiation.
BC017292 mRNA. Translation: AAH17292.2.
BT019478 mRNA. Translation: AAV38285.1.
CCDSiCCDS11245.2.
PIRiA53664.
RefSeqiNP_004466.2. NM_004475.2.
XP_005258009.1. XM_005257952.1.
UniGeneiHs.514038.

3D structure databases

ProteinModelPortaliQ14254.
SMRiQ14254. Positions 40-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108608. 85 interactions.
IntActiQ14254. 61 interactions.
MINTiMINT-1034434.
STRINGi9606.ENSP00000378368.

PTM databases

iPTMnetiQ14254.
PhosphoSiteiQ14254.
SwissPalmiQ14254.

Polymorphism and mutation databases

BioMutaiFLOT2.
DMDMi254763294.

Proteomic databases

EPDiQ14254.
MaxQBiQ14254.
PaxDbiQ14254.
PRIDEiQ14254.

Protocols and materials databases

DNASUi2319.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394908; ENSP00000378368; ENSG00000132589.
GeneIDi2319.
KEGGihsa:2319.
UCSCiuc002hdc.4. human.

Organism-specific databases

CTDi2319.
GeneCardsiFLOT2.
H-InvDBHIX0013665.
HGNCiHGNC:3758. FLOT2.
HPAiCAB004395.
HPA001396.
MIMi131560. gene.
neXtProtiNX_Q14254.
PharmGKBiPA28176.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ14254.
KOiK07192.
PhylomeDBiQ14254.
TreeFamiTF324879.

Miscellaneous databases

ChiTaRSiFLOT2. human.
GeneWikiiFLOT2.
GenomeRNAii2319.
NextBioi9417.
PROiQ14254.
SOURCEiSearch...

Gene expression databases

BgeeiQ14254.
CleanExiHS_FLOT2.
ExpressionAtlasiQ14254. baseline and differential.
GenevisibleiQ14254. HS.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Cloning and characterization of a novel epidermal cell surface antigen (ESA)."
    Schroeder W.T., Stewart-Galetka S., Mandavilli S., Parry D.A., Goldsmith L., Duvic M.
    J. Biol. Chem. 269:19983-19991(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-428.
    Tissue: Keratinocyte.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-428.
    Tissue: Pancreas.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-428.
  5. "A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components."
    Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M.
    J. Biol. Chem. 285:31616-31633(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ECM29.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells."
    Li Y., Martin B.R., Cravatt B.F., Hofmann S.L.
    J. Biol. Chem. 287:523-530(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-4; CYS-19 AND CYS-20, MUTAGENESIS OF CYS-4; CYS-19 AND CYS-20.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Global profiling of co- and post-translationally N-myristoylated proteomes in human cells."
    Thinon E., Serwa R.A., Broncel M., Brannigan J.A., Brassat U., Wright M.H., Heal W.P., Wilkinson A.J., Mann D.J., Tate E.W.
    Nat. Commun. 5:4919-4919(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiFLOT2_HUMAN
AccessioniPrimary (citable) accession number: Q14254
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 28, 2009
Last modified: April 13, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.