Q14241 (ELOA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor B polypeptide 3

Alternative name(s):
Elongin 110 kDa subunit
Elongin-A
Short name=EloA
RNA polymerase II transcription factor SIII subunit A1
SIII p110

Gene names

Name:	TCEB3
ORF Names:	MSTP059

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Subunit structure	Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A By similarity.
Subcellular location	Nucleus Probable.
Domain	The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Sequence similarities	Contains 1 F-box domain. Contains 1 TFIIS N-terminal domain.
Caution	It is uncertain whether Met-1 or Met-27 is the initiator.
Sequence caution	The sequence AAA75492.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH02883.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAO15305.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG35905.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAB40157.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence EAW95070.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of viral transcription Traceable author statement. Source: Reactome regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 7660129. Source: ProtInc transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome viral reproductive process Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: InterPro nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 798

798

Transcription elongation factor B polypeptide 3

PRO_0000086960

Regions

Domain

30 – 105

76

TFIIS N-terminal

Domain

592 – 636

45

F-box

Region

548 – 707

160

Activation domain By similarity

Region

576 – 585

10

Interacting with Elongin BC complex

Amino acid modifications

Modified residue

222

1

Phosphoserine By similarity

Modified residue

410

1

Phosphoserine Ref.7 Ref.8

Modified residue

413

1

Phosphoserine Ref.8

Modified residue

420

1

Phosphothreonine Ref.7

Natural variations

Natural variant

145

1

T → M.
Corresponds to variant rs2235541 [ dbSNP | Ensembl ].

VAR_020104

Natural variant

324

1

V → I.
Corresponds to variant rs520713 [ dbSNP | Ensembl ].

VAR_033850

Natural variant

516

1

A → V.
Corresponds to variant rs550252 [ dbSNP | Ensembl ].

VAR_033851

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

798

Helix Strand Turn

Details...

Helix

607 – 609

3

Helix

612 – 619

8

Helix

630 – 636

7

Beta strand

648 – 650

3

Helix

652 – 671

20

Sequences

	Sequence		Length	Mass (Da)
	Q14241 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 2C8FD8710E27EE46	FASTA	798	89,909
	10 20 30 40 50 60 MHGGRSCGPR TRREPSSGEE AAPVTAMAAE SALQVVEKLQ ARLAANPDPK KLLKYLKKLS 70 80 90 100 110 120 TLPITVDILA ETGVGKTVNS LRKHEHVGSF ARDLVAQWKK LVPVERNAEP DEQDFEKSNS 130 140 150 160 170 180 RKRPRDALQK EEEMEGDYQE TWKATGSRSY SPDHRQKKHR KLSELERPHK VSHGHERRDE 190 200 210 220 230 240 RKRCHRMSPT YSSDPESSDY GHVQSPPSCT SPHQMYVDHY RSLEEDQEPI VSHQKPGKGH 250 260 270 280 290 300 SNAFQDRLGA SQERHLGEPH GKGVVSQNKE HKSSHKDKRP VDAKSDEKAS VVSREKSHKA 310 320 330 340 350 360 LSKEENRRPP SGDNAREKPP SSGVKKEKDR EGSSLKKKCL PPSEAASDNH LKKPKHRDPE 370 380 390 400 410 420 KAKLDKSKQG LDSFDTGKGA GDLLPKVKEK GSNNLKTPEG KVKTNLDRKS LGSLPKVEET 430 440 450 460 470 480 DMEDEFEQPT MSFESYLSYD QPRKKKKKIV KTSATALGDK GLKKNDSKST GKNLDSVQKL 490 500 510 520 530 540 PKVNKTKSEK PAGADLAKLR KVPDVLPVLP DLPLPAIQAN YRPLPSLELI SSFQPKRKAF 550 560 570 580 590 600 SSPQEEEEAG FTGRRMNSKM QVYSGSKCAY LPKMMTLHQQ CIRVLKNNID SIFEVGGVPY 610 620 630 640 650 660 SVLEPVLERC TPDQLYRIEE YNHVLIEETD QLWKVHCHRD FKEERPEEYE SWREMYLRLQ 670 680 690 700 710 720 DAREQRLRVL TKNIQFAHAN KPKGRQAKMA FVNSVAKPPR DVRRRQEKFG TGGAAVPEKI 730 740 750 760 770 780 KIKPAPYPMG SSHASASSIS FNPSPEEPAY DGPSTSSAHL APVVSSTVSY DPRKPTVKKI 790 APMMAKTIKA FKNRFSRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"A human cDNA encoding the 110-kDa A subunit of RNA polymerase II transcription factor elongin." Aso T., Haque D., Fukudome K., Brower C.S., Conaway J.W., Conaway R.C. Gene 168:277-278(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-798. Tissue: Umbilical vein.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-798. Tissue: Lung.
[6]	Zhao B., Xu Y.Y., Liu Y.Q., Wang X.Y., Liu B., Ye J., Song L., Zhao Y., Cao H.Q., Zhao X.W., Gao Y., Liu L.S., Ding J.F., Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 32-798. Tissue: Heart.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410 AND THR-420, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410 AND SER-413, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK313079 mRNA. Translation: BAG35905.1. Different initiation.
DA333869 mRNA. No translation available.
AL031295 Genomic DNA. Translation: CAB40157.1. Different initiation.
CH471134 Genomic DNA. Translation: EAW95070.1. Different initiation.
L47345 mRNA. Translation: AAA75492.1. Different initiation.
BC002883 mRNA. Translation: AAH02883.1. Different initiation.
AF116729 mRNA. Translation: AAO15305.1. Different initiation.

IPI

IPI00941732.

PIR

JC4636.

RefSeq

NP_003189.2. NM_003198.2.

UniGene

Hs.15535.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HFX	X-ray	2.54	A/B/C/D	597-682	[»]

ProteinModelPortal

Q14241.

SMR

Q14241. Positions 600-673.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14241. 3 interactions.

MINT

MINT-1462212.

STRING

9606.ENSP00000395574.

PTM databases

PhosphoSite

Q14241.

Polymorphism databases

DMDM

294862430.

Proteomic databases

PaxDb

Q14241.

PeptideAtlas

Q14241.

PRIDE

Q14241.

Protocols and materials databases

DNASU

6924.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000418390; ENSP00000395574; ENSG00000011007.

GeneID

6924.

KEGG

hsa:6924.

UCSC

uc001bho.3. human.

Organism-specific databases

CTD

6924.

GeneCards

GC01P024069.

H-InvDB

HIX0000251.

HGNC

HGNC:11620. TCEB3.

HPA

HPA005910.

MIM

600786. gene.

neXtProt

NX_Q14241.

PharmGKB

PA36379.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG114994.

HOGENOM

HOG000059565.

HOVERGEN

HBG051467.

InParanoid

Q14241.

KO

K15076.

OMA

WKVHCHR.

OrthoDB

EOG495ZRB.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_1788. Transcription.
REACT_1892. Elongation arrest and recovery.
REACT_71. Gene Expression.

Gene expression databases

Bgee

Q14241.

Genevestigator

Q14241.

GermOnline

ENSG00000011007. Homo sapiens.

Family and domain databases

Gene3D

1.20.930.10. 1 hit.

InterPro

IPR001810. F-box_dom_cyclin-like.
IPR010684. RNA_pol_II_trans_fac_SIII_A.
IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]

Pfam

PF06881. Elongin_A. 1 hit.
PF08711. Med26. 1 hit.
[Graphical view]

SMART

SM00509. TFS2N. 1 hit.
[Graphical view]

SUPFAM

SSF47676. TFIIS_conserved. 1 hit.

PROSITE

PS50181. FBOX. 1 hit.
PS51319. TFIIS_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

6924.

NextBio

27089.

SOURCE

Search...

Entry information

Entry name

ELOA1_HUMAN

Accession

Primary (citable) accession number: Q14241
Secondary accession number(s): B2R7Q8, Q8IXH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	April 20, 2010
Last modified:	May 29, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.