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Q14240

- IF4A2_HUMAN

UniProt

Q14240 - IF4A2_HUMAN

Protein

Eukaryotic initiation factor 4A-II

Gene

EIF4A2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 2 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 848ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. helicase activity Source: ProtInc
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: IntAct
    6. translation initiation factor activity Source: ProtInc

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. cytokine-mediated signaling pathway Source: Reactome
    3. gene expression Source: Reactome
    4. mRNA metabolic process Source: Reactome
    5. nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: Reactome
    6. nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome
    7. regulation of translational initiation Source: ProtInc
    8. RNA metabolic process Source: Reactome
    9. translation Source: Reactome
    10. translational initiation Source: Reactome
    11. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase, Initiation factor

    Keywords - Biological processi

    Host-virus interaction, Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_115831. ISG15 antiviral mechanism.
    REACT_1258. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
    REACT_1979. Translation initiation complex formation.
    REACT_20514. Deadenylation of mRNA.
    REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
    REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
    REACT_931. Ribosomal scanning and start codon recognition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic initiation factor 4A-II (EC:3.6.4.13)
    Short name:
    eIF-4A-II
    Short name:
    eIF4A-II
    Alternative name(s):
    ATP-dependent RNA helicase eIF4A-2
    Gene namesi
    Name:EIF4A2
    Synonyms:DDX2B, EIF4F
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:3284. EIF4A2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. eukaryotic translation initiation factor 4F complex Source: ProtInc

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27712.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Eukaryotic initiation factor 4A-IIPRO_0000054938Add
    BLAST

    Proteomic databases

    MaxQBiQ14240.
    PaxDbiQ14240.
    PRIDEiQ14240.

    PTM databases

    PhosphoSiteiQ14240.

    Miscellaneous databases

    PMAP-CutDBQ14240.

    Expressioni

    Gene expression databases

    ArrayExpressiQ14240.
    BgeeiQ14240.
    CleanExiHS_EIF4A2.
    GenevestigatoriQ14240.

    Organism-specific databases

    HPAiCAB011690.

    Interactioni

    Subunit structurei

    eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3 By similarity. Interacts with EIF4E. May interact with NOM1. Interacts with HHV-1 Vhs.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P279584EBI-73473,EBI-6904388From a different organism.

    Protein-protein interaction databases

    BioGridi108290. 41 interactions.
    IntActiQ14240. 18 interactions.
    MINTiMINT-202114.
    STRINGi9606.ENSP00000326381.

    Structurei

    Secondary structure

    1
    407
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 373
    Helixi42 – 5110
    Helixi58 – 6811
    Beta strandi73 – 753
    Helixi81 – 9414
    Beta strandi104 – 1074
    Helixi111 – 12414
    Turni125 – 1295
    Beta strandi132 – 1354
    Beta strandi154 – 1585
    Helixi160 – 1689
    Beta strandi179 – 1846
    Helixi185 – 1906
    Helixi194 – 20310
    Beta strandi209 – 2135
    Helixi219 – 22810
    Beta strandi233 – 2353

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BORX-ray1.85A22-240[»]
    ProteinModelPortaliQ14240.
    SMRiQ14240. Positions 23-401.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ14240.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini64 – 235172Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini246 – 407162Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi33 – 6129Q motifAdd
    BLAST
    Motifi183 – 1864DEAD box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOGENOMiHOG000268797.
    HOVERGENiHBG107989.
    InParanoidiQ14240.
    KOiK03257.
    OMAiMQLNFPH.
    PhylomeDBiQ14240.
    TreeFamiTF101524.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14240-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA    50
    YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK 100
    ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA 150
    EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI 200
    FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF 250
    YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV 300
    SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL 350
    PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP 400
    MNVADLI 407
    Length:407
    Mass (Da):46,402
    Last modified:March 15, 2004 - v2
    Checksum:iFAA7D3BA9D8C6DA0
    GO
    Isoform 2 (identifier: Q14240-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         9-9: N → NS

    Show »
    Length:408
    Mass (Da):46,489
    Checksum:i0F47C0C4EEF00DD3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271N → S in AAH15842. (PubMed:15489334)Curated
    Sequence conflicti212 – 2132LL → FA in BAA06336. (PubMed:8521730)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti93 – 931Q → H.
    Corresponds to variant rs11538616 [ dbSNP | Ensembl ].
    VAR_052158
    Natural varianti181 – 1811V → L in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035838

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei9 – 91N → NS in isoform 2. 1 PublicationVSP_009629

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30655 mRNA. Translation: BAA06336.1.
    BT009860 mRNA. Translation: AAP88862.1.
    AC112907 Genomic DNA. No translation available.
    CH471052 Genomic DNA. Translation: EAW78172.1.
    CH471052 Genomic DNA. Translation: EAW78177.1.
    BC012547 mRNA. Translation: AAH12547.1.
    BC013708 mRNA. Translation: AAH13708.1.
    BC015842 mRNA. Translation: AAH15842.1.
    BC048105 mRNA. Translation: AAH48105.1.
    CCDSiCCDS3282.1. [Q14240-1]
    RefSeqiNP_001958.2. NM_001967.3. [Q14240-1]
    UniGeneiHs.518475.
    Hs.599481.

    Genome annotation databases

    EnsembliENST00000323963; ENSP00000326381; ENSG00000156976. [Q14240-1]
    ENST00000440191; ENSP00000398370; ENSG00000156976. [Q14240-2]
    GeneIDi1974.
    KEGGihsa:1974.
    UCSCiuc003fqs.3. human. [Q14240-1]
    uc003fqu.3. human. [Q14240-2]

    Polymorphism databases

    DMDMi45645183.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30655 mRNA. Translation: BAA06336.1 .
    BT009860 mRNA. Translation: AAP88862.1 .
    AC112907 Genomic DNA. No translation available.
    CH471052 Genomic DNA. Translation: EAW78172.1 .
    CH471052 Genomic DNA. Translation: EAW78177.1 .
    BC012547 mRNA. Translation: AAH12547.1 .
    BC013708 mRNA. Translation: AAH13708.1 .
    BC015842 mRNA. Translation: AAH15842.1 .
    BC048105 mRNA. Translation: AAH48105.1 .
    CCDSi CCDS3282.1. [Q14240-1 ]
    RefSeqi NP_001958.2. NM_001967.3. [Q14240-1 ]
    UniGenei Hs.518475.
    Hs.599481.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BOR X-ray 1.85 A 22-240 [» ]
    ProteinModelPortali Q14240.
    SMRi Q14240. Positions 23-401.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108290. 41 interactions.
    IntActi Q14240. 18 interactions.
    MINTi MINT-202114.
    STRINGi 9606.ENSP00000326381.

    PTM databases

    PhosphoSitei Q14240.

    Polymorphism databases

    DMDMi 45645183.

    Proteomic databases

    MaxQBi Q14240.
    PaxDbi Q14240.
    PRIDEi Q14240.

    Protocols and materials databases

    DNASUi 1974.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000323963 ; ENSP00000326381 ; ENSG00000156976 . [Q14240-1 ]
    ENST00000440191 ; ENSP00000398370 ; ENSG00000156976 . [Q14240-2 ]
    GeneIDi 1974.
    KEGGi hsa:1974.
    UCSCi uc003fqs.3. human. [Q14240-1 ]
    uc003fqu.3. human. [Q14240-2 ]

    Organism-specific databases

    CTDi 1974.
    GeneCardsi GC03P186556.
    H-InvDB HIX0003899.
    HGNCi HGNC:3284. EIF4A2.
    HPAi CAB011690.
    MIMi 601102. gene.
    neXtProti NX_Q14240.
    PharmGKBi PA27712.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0513.
    HOGENOMi HOG000268797.
    HOVERGENi HBG107989.
    InParanoidi Q14240.
    KOi K03257.
    OMAi MQLNFPH.
    PhylomeDBi Q14240.
    TreeFami TF101524.

    Enzyme and pathway databases

    Reactomei REACT_115831. ISG15 antiviral mechanism.
    REACT_1258. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
    REACT_1979. Translation initiation complex formation.
    REACT_20514. Deadenylation of mRNA.
    REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
    REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
    REACT_931. Ribosomal scanning and start codon recognition.

    Miscellaneous databases

    ChiTaRSi EIF4A2. human.
    EvolutionaryTracei Q14240.
    GeneWikii EIF4A2.
    GenomeRNAii 1974.
    NextBioi 7991.
    PMAP-CutDB Q14240.
    PROi Q14240.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14240.
    Bgeei Q14240.
    CleanExi HS_EIF4A2.
    Genevestigatori Q14240.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2."
      Sudo K., Takahashi E., Nakamura Y.
      Cytogenet. Cell Genet. 71:385-388(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Lung.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Bone marrow, Brain, Lymph and Skeletal muscle.
    6. "Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo."
      Li W., Belsham G.J., Proud C.G.
      J. Biol. Chem. 276:29111-29115(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EIF4E.
    7. "Identification of NOM1, a nucleolar, eIF4A binding protein encoded within the chromosome 7q36 breakpoint region targeted in cases of pediatric acute myeloid leukemia."
      Simmons H.M., Ruis B.L., Kapoor M., Hudacek A.W., Conklin K.F.
      Gene 347:137-145(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE INTERACTION WITH NOM1.
    8. "mRNA decay during herpes simplex virus (HSV) infections: protein-protein interactions involving the HSV virion host shutoff protein and translation factors eIF4H and eIF4A."
      Feng P., Everly D.N. Jr., Read G.S.
      J. Virol. 79:9651-9664(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HHV-1 VHS.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 22-240.
    11. Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-181.

    Entry informationi

    Entry nameiIF4A2_HUMAN
    AccessioniPrimary (citable) accession number: Q14240
    Secondary accession number(s): D3DNU9
    , Q53XJ6, Q96B90, Q96EA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Translation initiation factors
      List of translation initiation factor entries
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3