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Protein

Eukaryotic initiation factor 4A-II

Gene

EIF4A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: AgBase
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • helicase activity Source: ProtInc
  • poly(A) RNA binding Source: UniProtKB
  • translation initiation factor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Host-virus interaction, Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156976-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiQ14240.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-II (EC:3.6.4.13)
Short name:
eIF-4A-II
Short name:
eIF4A-II
Alternative name(s):
ATP-dependent RNA helicase eIF4A-2
Gene namesi
Name:EIF4A2
Synonyms:DDX2B, EIF4F
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:3284. EIF4A2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • eukaryotic translation initiation factor 4F complex Source: ProtInc
  • perinuclear region of cytoplasm Source: AgBase
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1974.
OpenTargetsiENSG00000156976.
PharmGKBiPA27712.

Polymorphism and mutation databases

BioMutaiEIF4A2.
DMDMi45645183.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549381 – 407Eukaryotic initiation factor 4A-IIAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14240.
MaxQBiQ14240.
PaxDbiQ14240.
PeptideAtlasiQ14240.
PRIDEiQ14240.

PTM databases

iPTMnetiQ14240.
PhosphoSitePlusiQ14240.
SwissPalmiQ14240.

Miscellaneous databases

PMAP-CutDBQ14240.

Expressioni

Gene expression databases

BgeeiENSG00000156976.
CleanExiHS_EIF4A2.
ExpressionAtlasiQ14240. baseline and differential.
GenevisibleiQ14240. HS.

Organism-specific databases

HPAiCAB011690.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3 (By similarity). Interacts with EIF4E. May interact with NOM1. Interacts with HHV-1 Vhs.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P279584EBI-73473,EBI-6904388From a different organism.
DPPA4Q7L1903EBI-73473,EBI-710457
HSPB1P047922EBI-73473,EBI-352682
IPO11Q9UI263EBI-73473,EBI-748752
MDFIQ997503EBI-10232522,EBI-724076
PDCD4Q53EL65EBI-73473,EBI-935824
PIH1D2Q8WWB53EBI-10232522,EBI-10232538
SLC16A9Q7RTY13EBI-73473,EBI-10232636
SSX2IPQ9Y2D83EBI-73473,EBI-2212028
TRIM36Q9NQ863EBI-73473,EBI-2341518
TRIM39Q9HCM93EBI-10232522,EBI-739510

Protein-protein interaction databases

BioGridi108290. 62 interactors.
IntActiQ14240. 28 interactors.
MINTiMINT-202114.
STRINGi9606.ENSP00000326381.

Structurei

Secondary structure

1407
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 37Combined sources3
Helixi42 – 51Combined sources10
Helixi58 – 68Combined sources11
Beta strandi73 – 75Combined sources3
Helixi81 – 94Combined sources14
Beta strandi104 – 107Combined sources4
Helixi111 – 124Combined sources14
Turni125 – 129Combined sources5
Beta strandi132 – 135Combined sources4
Beta strandi154 – 158Combined sources5
Helixi160 – 168Combined sources9
Beta strandi179 – 184Combined sources6
Helixi185 – 190Combined sources6
Helixi194 – 203Combined sources10
Beta strandi209 – 213Combined sources5
Helixi219 – 228Combined sources10
Beta strandi233 – 235Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BORX-ray1.85A22-240[»]
ProteinModelPortaliQ14240.
SMRiQ14240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14240.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 235Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini246 – 407Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi33 – 61Q motifAdd BLAST29
Motifi183 – 186DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ14240.
KOiK03257.
OMAiNEITDNF.
OrthoDBiEOG091G07OI.
PhylomeDBiQ14240.
TreeFamiTF101524.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14240-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA
60 70 80 90 100
YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK
110 120 130 140 150
ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA
160 170 180 190 200
EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI
210 220 230 240 250
FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF
260 270 280 290 300
YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV
310 320 330 340 350
SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL
360 370 380 390 400
PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP

MNVADLI
Length:407
Mass (Da):46,402
Last modified:March 15, 2004 - v2
Checksum:iFAA7D3BA9D8C6DA0
GO
Isoform 2 (identifier: Q14240-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-9: N → NS

Show »
Length:408
Mass (Da):46,489
Checksum:i0F47C0C4EEF00DD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27N → S in AAH15842 (PubMed:15489334).Curated1
Sequence conflicti212 – 213LL → FA in BAA06336 (PubMed:8521730).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05215893Q → H.Corresponds to variant rs11538616dbSNPEnsembl.1
Natural variantiVAR_035838181V → L in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096299N → NS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30655 mRNA. Translation: BAA06336.1.
BT009860 mRNA. Translation: AAP88862.1.
AC112907 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78172.1.
CH471052 Genomic DNA. Translation: EAW78177.1.
BC012547 mRNA. Translation: AAH12547.1.
BC013708 mRNA. Translation: AAH13708.1.
BC015842 mRNA. Translation: AAH15842.1.
BC048105 mRNA. Translation: AAH48105.1.
CCDSiCCDS3282.1. [Q14240-1]
RefSeqiNP_001958.2. NM_001967.3. [Q14240-1]
UniGeneiHs.518475.
Hs.599481.

Genome annotation databases

EnsembliENST00000323963; ENSP00000326381; ENSG00000156976. [Q14240-1]
ENST00000440191; ENSP00000398370; ENSG00000156976. [Q14240-2]
GeneIDi1974.
KEGGihsa:1974.
UCSCiuc003fqs.4. human. [Q14240-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30655 mRNA. Translation: BAA06336.1.
BT009860 mRNA. Translation: AAP88862.1.
AC112907 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78172.1.
CH471052 Genomic DNA. Translation: EAW78177.1.
BC012547 mRNA. Translation: AAH12547.1.
BC013708 mRNA. Translation: AAH13708.1.
BC015842 mRNA. Translation: AAH15842.1.
BC048105 mRNA. Translation: AAH48105.1.
CCDSiCCDS3282.1. [Q14240-1]
RefSeqiNP_001958.2. NM_001967.3. [Q14240-1]
UniGeneiHs.518475.
Hs.599481.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BORX-ray1.85A22-240[»]
ProteinModelPortaliQ14240.
SMRiQ14240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108290. 62 interactors.
IntActiQ14240. 28 interactors.
MINTiMINT-202114.
STRINGi9606.ENSP00000326381.

PTM databases

iPTMnetiQ14240.
PhosphoSitePlusiQ14240.
SwissPalmiQ14240.

Polymorphism and mutation databases

BioMutaiEIF4A2.
DMDMi45645183.

Proteomic databases

EPDiQ14240.
MaxQBiQ14240.
PaxDbiQ14240.
PeptideAtlasiQ14240.
PRIDEiQ14240.

Protocols and materials databases

DNASUi1974.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323963; ENSP00000326381; ENSG00000156976. [Q14240-1]
ENST00000440191; ENSP00000398370; ENSG00000156976. [Q14240-2]
GeneIDi1974.
KEGGihsa:1974.
UCSCiuc003fqs.4. human. [Q14240-1]

Organism-specific databases

CTDi1974.
DisGeNETi1974.
GeneCardsiEIF4A2.
H-InvDBHIX0003899.
HGNCiHGNC:3284. EIF4A2.
HPAiCAB011690.
MIMi601102. gene.
neXtProtiNX_Q14240.
OpenTargetsiENSG00000156976.
PharmGKBiPA27712.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ14240.
KOiK03257.
OMAiNEITDNF.
OrthoDBiEOG091G07OI.
PhylomeDBiQ14240.
TreeFamiTF101524.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156976-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiQ14240.

Miscellaneous databases

ChiTaRSiEIF4A2. human.
EvolutionaryTraceiQ14240.
GeneWikiiEIF4A2.
GenomeRNAii1974.
PMAP-CutDBQ14240.
PROiQ14240.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156976.
CleanExiHS_EIF4A2.
ExpressionAtlasiQ14240. baseline and differential.
GenevisibleiQ14240. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4A2_HUMAN
AccessioniPrimary (citable) accession number: Q14240
Secondary accession number(s): D3DNU9
, Q53XJ6, Q96B90, Q96EA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 176 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Translation initiation factors
    List of translation initiation factor entries
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.