ID   IL27B_HUMAN             Reviewed;         229 AA.
AC   Q14213; O75269;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2004, sequence version 2.
DT   24-NOV-2009, entry version 78.
DE   RecName: Full=Interleukin-27 subunit beta;
DE            Short=IL-27 subunit beta;
DE            Short=IL-27B;
DE   AltName: Full=Epstein-Barr virus-induced gene 3 protein;
DE            Short=EBV-induced gene 3 protein;
DE   Flags: Precursor;
GN   Name=EBI3; Synonyms=IL27B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GLYCOSYLATION, AND INTERACTION
RP   WITH SQSTM1.
RC   TISSUE=B-cell;
RX   MEDLINE=96135230; PubMed=8551575;
RA   Devergne O., Hummel M., Koeppen H., Le Beau M.M., Nathanson E.C.,
RA   Kieff E., Birkenbach M.;
RT   "A novel interleukin-12 p40-related protein induced by latent Epstein-
RT   Barr virus infection in B lymphocytes.";
RL   J. Virol. 70:1143-1153(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J.,
RA   Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M.,
RA   Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E.,
RA   Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M.,
RA   Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C.,
RA   Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M.,
RA   Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H.,
RA   Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S.,
RA   Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J.,
RA   Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M.,
RA   Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J.,
RA   Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D.,
RA   Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A.,
RA   Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I.,
RA   Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=12121660; DOI=10.1016/S1074-7613(02)00324-2;
RA   Pflanz S., Timans J.C., Cheung J., Rosales R., Kanzler H., Gilbert J.,
RA   Hibbert L., Churakova T., Travis M., Vaisberg E., Blumenschein W.M.,
RA   Mattson J.D., Wagner J.L., To W., Zurawski S., McClanahan T.K.,
RA   Gorman D.M., Bazan J.F., de Waal Malefyt R., Rennick D.,
RA   Kastelein R.A.;
RT   "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein,
RT   induces proliferation of naive CD4(+) T cells.";
RL   Immunity 16:779-790(2002).
RN   [5]
RP   REVIEW.
RX   PubMed=17294231; DOI=10.1007/s00109-007-0164-7;
RA   Batten M., Ghilardi N.;
RT   "The biology and therapeutic potential of interleukin 27.";
RL   J. Mol. Med. 85:661-672(2007).
CC   -!- FUNCTION: Cytokine with pro- and anti-inflammatory properties,
CC       that can regulate T helper cell development, suppress T-cell
CC       proliferation, stimulate cytotoxic T cell activity, induce isotype
CC       switching in B-cells, and that has diverse effects on innate
CC       immune cells. Among its target cells are CD4 T helper cells which
CC       can differentiate in type 1 effector cells (TH1), type 2 effector
CC       cells (TH2) and IL17 producing helper T-cells (TH17). It drives
CC       rapid clonal expansion of naive but not memory CD4 T-cells. It
CC       also strongly synergizes with IL-12 to trigger interferon-
CC       gamma/IFN-gamma production of naive CD4 T-cells, binds to the
CC       cytokine receptor WSX-1/TCCR. Another important role of IL27 is
CC       its antitumor activity as well as its antiangiogenic activity with
CC       activation of production of antiangiogenic chemokines.
CC   -!- SUBUNIT: Heterodimer with IL27. IL-27 is an heterodimer composed
CC       IL27 and EBI3. EBI3 is also a component of the IL-12 heterodimer.
CC       Interacts with SQSTM1.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- INDUCTION: By EBV.
CC   -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 3
CC       subfamily.
CC   -!- SIMILARITY: Contains 2 fibronectin type-III domains.
CC   -!- WEB RESOURCE: Name=Wikipedia; Note=Interleukin-27 entry;
CC       URL="http://en.wikipedia.org/wiki/Interleukin_27";
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; L08187; AAA93193.1; -; mRNA.
DR   EMBL; AC005578; AAC33488.1; -; Genomic_DNA.
DR   EMBL; BC015364; AAH15364.1; -; mRNA.
DR   EMBL; BC046112; AAH46112.1; -; mRNA.
DR   IPI; IPI00034088; -.
DR   RefSeq; NP_005746.2; -.
DR   UniGene; Hs.501452; -.
DR   IntAct; Q14213; 1.
DR   STRING; Q14213; -.
DR   PRIDE; Q14213; -.
DR   Ensembl; ENST00000221847; ENSP00000221847; ENSG00000105246; Homo sapiens.
DR   GeneID; 10148; -.
DR   KEGG; hsa:10148; -.
DR   UCSC; uc002lzu.1; human.
DR   CTD; 10148; -.
DR   GeneCards; GC19P004180; -.
DR   H-InvDB; HIX0014656; -.
DR   HGNC; HGNC:3129; EBI3.
DR   MIM; 605816; gene.
DR   PharmGKB; PA27584; -.
DR   HOGENOM; Q14213; -.
DR   HOVERGEN; Q14213; -.
DR   OMA; YPVAVDC; -.
DR   OrthoDB; EOG9ZGRZ2; -.
DR   Pathway_Interaction_DB; il27pathway; IL27-mediated signaling events.
DR   NextBio; 38403; -.
DR   ArrayExpress; Q14213; -.
DR   Bgee; Q14213; -.
DR   CleanEx; HS_EBI3; -.
DR   Genevestigator; Q14213; -.
DR   GermOnline; ENSG00000105246; Homo sapiens.
DR   GO; GO:0005615; C:extracellular space; TAS:ProtInc.
DR   GO; GO:0005886; C:plasma membrane; TAS:ProtInc.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0004896; F:cytokine receptor activity; IPI:UniProtKB.
DR   GO; GO:0006959; P:humoral immune response; TAS:ProtInc.
DR   GO; GO:0046641; P:positive regulation of alpha-beta T cell pr...; TAS:UniProtKB.
DR   GO; GO:0045078; P:positive regulation of interferon-gamma bio...; TAS:UniProtKB.
DR   GO; GO:0042088; P:T-helper 1 type immune response; TAS:UniProtKB.
DR   InterPro; IPR008957; Fibronectin_typ-III-like_fold.
DR   InterPro; IPR003961; FN_III.
DR   InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR   Gene3D; G3DSA:2.60.40.30; FN_III-like; 1.
DR   Pfam; PF00041; fn3; 1.
DR   SMART; SM00060; FN3; 2.
DR   PROSITE; PS50853; FN3; 2.
DR   PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytokine; Glycoprotein; Polymorphism; Repeat;
KW   Secreted; Signal.
FT   SIGNAL        1     20       Potential.
FT   CHAIN        21    229       Interleukin-27 subunit beta.
FT                                /FTId=PRO_0000010937.
FT   DOMAIN       24    124       Fibronectin type-III 1.
FT   DOMAIN      129    221       Fibronectin type-III 2.
FT   CARBOHYD     55     55       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    105    105       N-linked (GlcNAc...) (Potential).
FT   VARIANT     174    174       A -> V (in dbSNP:rs1803524).
FT                                /FTId=VAR_049171.
FT   VARIANT     201    201       V -> I (in dbSNP:rs4740).
FT                                /FTId=VAR_024342.
FT   CONFLICT    144    145       QL -> HV (in Ref. 1; AAA93193).
SQ   SEQUENCE   229 AA;  25396 MW;  CFBAD72D91859EF0 CRC64;
     MTPQLLLALV LWASCPPCSG RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV
     SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP
     FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV
     GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK
//