Reviewed, UniProtKB/Swiss-Prot Q14213 (IL27B_HUMAN)

Names and origin

Protein names

Recommended name:
Interleukin-27 subunit beta
Short name=IL-27 subunit beta
Short name=IL-27B

Alternative name(s):
Epstein-Barr virus-induced gene 3 protein
Short name=EBV-induced gene 3 protein

Gene names

Name:	EBI3
Synonyms:	IL27B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Ref.6
Subunit structure	Heterodimer with IL27. IL-27 is an heterodimer composed IL27 and EBI3. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1. Ref.6 Ref.1
Subcellular location	Secreted.
Induction	By EBV. Ref.1
Sequence similarities	Belongs to the type I cytokine receptor family. Type 3 subfamily. Contains 2 fibronectin type-III domains.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
Molecular function	Cytokine
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	T-helper 1 type immune response Traceable author statement. Source: UniProtKB humoral immune response Ref.1 Traceable author statement. Source: ProtInc positive regulation of alpha-beta T cell proliferation Traceable author statement. Source: UniProtKB positive regulation of interferon-gamma biosynthetic process Traceable author statement. Source: UniProtKB
Cellular component	extracellular space Ref.1 Traceable author statement. Source: ProtInc plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW cytokine receptor activity Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 229	209	Interleukin-27 subunit beta		PRO_0000010937
Regions
	Domain	24 – 124	101	Fibronectin type-III 1
	Domain	129 – 221	93	Fibronectin type-III 2
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	174	1	A → V: dbSNP rs1803524.		VAR_049171
	Natural variant	201	1	V → I: dbSNP rs4740. Ref.2		VAR_024342
Experimental info
	Sequence conflict	144 – 145	2	QL → HV in AAA93193. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q14213-1 [UniParc]. Last modified May 24, 2004. Version 2. Checksum: CFBAD72D91859EF0	FASTA	229	25,396
	10 20 30 40 50 60 MTPQLLLALV LWASCPPCSG RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV 70 80 90 100 110 120 SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP 130 140 150 160 170 180 FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV 190 200 210 220 GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes." Devergne O., Hummel M., Koeppen H., Le Beau M.M., Nathanson E.C., Kieff E., Birkenbach M. J. Virol. 70:1143-1153(1996) [PubMed: 8551575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GLYCOSYLATION, INTERACTION WITH SQSTM1. Tissue: B-cell.
[2]	Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ILE-201.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-201.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[6]	"IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells." Pflanz S., Timans J.C., Cheung J., Rosales R., Kanzler H., Gilbert J., Hibbert L., Churakova T., Travis M., Vaisberg E., Blumenschein W.M., Mattson J.D., Wagner J.L., To W., Zurawski S., McClanahan T.K., Gorman D.M., Bazan J.F. , de Waal Malefyt R., Rennick D., Kastelein R.A. Immunity 16:779-790(2002) [PubMed: 12121660] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"The biology and therapeutic potential of interleukin 27." Batten M., Ghilardi N. J. Mol. Med. 85:661-672(2007) [PubMed: 17294231] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

Wikipedia

Interleukin-27 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08187 mRNA. Translation: AAA93193.1. EF064740 Genomic DNA. Translation: ABK41923.1. AC005578 Genomic DNA. Translation: AAC33488.1. CH471139 Genomic DNA. Translation: EAW69245.1. BC015364 mRNA. Translation: AAH15364.1. BC046112 mRNA. Translation: AAH46112.1.
IPI	IPI00034088.
RefSeq	NP_005746.2.
UniGene	Hs.501452
3D structure databases
SMR	Q14213. Positions 30-221.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14213. 1 interaction.
STRING	Q14213.
Proteomic databases
PRIDE	Q14213.
Genome annotation databases
Ensembl	ENST00000221847; ENSP00000221847; ENSG00000105246; Homo sapiens. [Genome view]
GeneID	10148.
KEGG	hsa:10148.
UCSC	uc002lzu.1. human.
Organism-specific databases
CTD	10148.
GeneCards	GC19P004180.
H-InvDB	HIX0014656.
HGNC	HGNC:3129. EBI3.
MIM	605816. gene.
PharmGKB	PA27584.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG269481.
HOVERGEN	HBG052107.
InParanoid	Q14213.
OMA	YPVAVDC.
OrthoDB	EOG9ZGRZ2.
PhylomeDB	Q14213.
Enzyme and pathway databases
Pathway_Interaction_DB	il27pathway. IL27-mediated signaling events.
Gene expression databases
ArrayExpress	Q14213.
Bgee	Q14213.
CleanEx	HS_EBI3.
Genevestigator	Q14213.
GermOnline	ENSG00000105246. Homo sapiens.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003530. Hematopoietin_rcpt_L_F3_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit.
Pfam	PF00041. fn3. 1 hit. [Graphical view]
SMART	SM00060. FN3. 2 hits. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 2 hits.
PROSITE	PS50853. FN3. 2 hits. PS01354. HEMATOPO_REC_L_F3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	38403.
SOURCE	Search...

Entry information

Entry name

IL27B_HUMAN

Accession

Primary (citable) accession number: Q14213
Secondary accession number(s): A0N0N2, O75269

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	March 2, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.