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Q14203

- DCTN1_HUMAN

UniProt

Q14203 - DCTN1_HUMAN

Protein

Dynactin subunit 1

Gene

DCTN1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 3 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

    GO - Molecular functioni

    1. motor activity Source: UniProtKB-KW
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
    2. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
    3. cell death Source: UniProtKB-KW
    4. cellular protein metabolic process Source: Reactome
    5. endoplasmic reticulum unfolded protein response Source: Reactome
    6. G2/M transition of mitotic cell cycle Source: Reactome
    7. microtubule-based transport Source: InterPro
    8. mitotic cell cycle Source: Reactome
    9. mitotic nuclear division Source: ProtInc
    10. nervous system development Source: UniProtKB

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_121399. MHC class II antigen presentation.
    REACT_15296. Recruitment of mitotic centrosome proteins and complexes.
    REACT_15364. Loss of Nlp from mitotic centrosomes.
    REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
    REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
    REACT_18273. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynactin subunit 1
    Alternative name(s):
    150 kDa dynein-associated polypeptide
    DAP-150
    Short name:
    DP-150
    p135
    p150-glued
    Gene namesi
    Name:DCTN1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:2711. DCTN1.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Colocalizes with microtubules.

    GO - Cellular componenti

    1. cell leading edge Source: Ensembl
    2. centrosome Source: UniProtKB
    3. cytoplasm Source: ProtInc
    4. cytosol Source: Reactome
    5. dynactin complex Source: InterPro
    6. dynein complex Source: UniProtKB-KW
    7. kinetochore Source: UniProtKB
    8. membrane Source: UniProtKB
    9. microtubule Source: UniProtKB
    10. spindle pole Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Microtubule

    Pathology & Biotechi

    Involvement in diseasei

    Neuronopathy, distal hereditary motor, 7B (HMN7B) [MIM:607641]: A neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti59 – 591G → S in HMN7B; shows a modestly reduced affinity for microtubules which has been suggested to impair axonal transport; the effect is identical to that of complete loss of the CAP-Gly domain. 1 Publication
    VAR_015850
    Amyotrophic lateral sclerosis (ALS) [MIM:105400]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.2 Publications
    Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Perry syndrome (PERRYS) [MIM:168605]: A neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti71 – 711G → A in PERRYS. 1 Publication
    VAR_063867
    Natural varianti71 – 711G → E in PERRYS. 1 Publication
    VAR_063868
    Natural varianti71 – 711G → R in PERRYS; diminishes microtubule binding and lead to intracytoplasmic inclusions. 1 Publication
    VAR_063869
    Natural varianti72 – 721T → P in PERRYS. 1 Publication
    VAR_063870
    Natural varianti74 – 741Q → P in PERRYS; diminishes microtubule binding and lead to intracytoplasmic inclusions. 1 Publication
    VAR_063871

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi68 – 681K → A: Abolishes interaction with CLIP1. 1 Publication
    Mutagenesisi90 – 901R → E: Abolishes interaction with CLIP1. 1 Publication

    Keywords - Diseasei

    Amyotrophic lateral sclerosis, Neurodegeneration, Parkinsonism

    Organism-specific databases

    MIMi105400. phenotype.
    168605. phenotype.
    607641. phenotype.
    Orphaneti803. Amyotrophic lateral sclerosis.
    139589. Distal hereditary motor neuropathy type 7.
    178509. Perry syndrome.
    PharmGKBiPA27180.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12781278Dynactin subunit 1PRO_0000083518Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei108 – 1081Phosphothreonine1 Publication

    Post-translational modificationi

    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ14203.
    PaxDbiQ14203.
    PRIDEiQ14203.

    2D gel databases

    OGPiQ14203.

    PTM databases

    PhosphoSiteiQ14203.

    Miscellaneous databases

    PMAP-CutDBQ14203.

    Expressioni

    Tissue specificityi

    Brain.

    Gene expression databases

    ArrayExpressiQ14203.
    BgeeiQ14203.
    CleanExiHS_DCTN1.
    GenevestigatoriQ14203.

    Organism-specific databases

    HPAiCAB009108.
    HPA034635.

    Interactioni

    Subunit structurei

    Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts (via C-terminus) with SNX6. Interacts with CLIP1, CLN3, DYNAP, ECM29 and FBXL5. Interacts with MISP; this interaction regulates its distribution at the cell cortex. Interacts with CEP131.12 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BBS4Q96RK43EBI-724352,EBI-1805814
    Hap1P542564EBI-724352,EBI-994539From a different organism.
    MAPTP10636-88EBI-724352,EBI-366233

    Protein-protein interaction databases

    BioGridi108007. 58 interactions.
    DIPiDIP-31365N.
    IntActiQ14203. 42 interactions.
    MINTiMINT-5004548.
    STRINGi9606.ENSP00000354791.

    Structurei

    Secondary structure

    1
    1278
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi17 – 193
    Beta strandi32 – 354
    Turni36 – 383
    Beta strandi41 – 488
    Beta strandi51 – 555
    Beta strandi57 – 659
    Beta strandi67 – 737
    Beta strandi76 – 783
    Turni83 – 853
    Beta strandi86 – 894
    Helixi91 – 933
    Beta strandi94 – 963

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TXQX-ray1.80A15-107[»]
    2COYNMR-A1-99[»]
    2HKNX-ray1.87A/B18-111[»]
    2HKQX-ray1.86B18-111[»]
    2HL3X-ray2.03A/B18-111[»]
    2HL5X-ray1.93C/D18-111[»]
    2HQHX-ray1.80A/B/C/D15-107[»]
    3E2UX-ray2.60A/B/C/D18-111[»]
    3TQ7X-ray2.30P/Q27-97[»]
    ProteinModelPortaliQ14203.
    SMRiQ14203. Positions 25-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ14203.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 9043CAP-GlyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili213 – 547335Sequence AnalysisAdd
    BLAST
    Coiled coili943 – 1049107Sequence AnalysisAdd
    BLAST
    Coiled coili1182 – 121130Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi164 – 19128Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dynactin 150 kDa subunit family.Curated
    Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5244.
    HOGENOMiHOG000015352.
    HOVERGENiHBG004956.
    KOiK04648.
    OMAiPEDSTMQ.
    OrthoDBiEOG79W94B.
    PhylomeDBiQ14203.
    TreeFamiTF105246.

    Family and domain databases

    Gene3Di2.30.30.190. 1 hit.
    InterProiIPR000938. CAP-Gly_domain.
    IPR027663. DCTN1.
    IPR022157. Dynactin.
    [Graphical view]
    PANTHERiPTHR18916:SF29. PTHR18916:SF29. 1 hit.
    PfamiPF01302. CAP_GLY. 1 hit.
    PF12455. Dynactin. 1 hit.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 1 hit.
    [Graphical view]
    SUPFAMiSSF74924. SSF74924. 1 hit.
    PROSITEiPS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform p150 (identifier: Q14203-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQSKRHVYS RTPSGSRMSA EASARPLRVG SRVEVIGKGH RGTVAYVGAT     50
    LFATGKWVGV ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG 100
    ADTTSPETPD SSASKVLKRE GTDTTAKTSK LRGLKPKKAP TARKTTTRRP 150
    KPTRPASTGV AGASSSLGPS GSASAGELSS SEPSTPAQTP LAAPIIPTPV 200
    LTSPGAVPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRAE DKAKLKELEK 250
    HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 300
    ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG 350
    SDGAASSYQL KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ 400
    ELEVVRQQRE RLQEELSQAE STIDELKEQV DAALGAEEMV EMLTDRNLNL 450
    EEKVRELRET VGDLEAMNEM NDELQENARE TELELREQLD MAGARVREAQ 500
    KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA SVERQQQPPP 550
    ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 600
    GDHDCVLVLL LMPRLICKAE LIRKQAQEKF ELSENCSERP GLRGAAGEQL 650
    SFAAGLVYSL SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD 700
    FLIELLHKDQ LDETVNVEPL TKAIKYYQHL YSIHLAEQPE DCTMQLADHI 750
    KFTQSALDCM SVEVGRLRAF LQGGQEATDI ALLLRDLETS CSDIRQFCKK 800
    IRRRMPGTDA PGIPAALAFG PQVSDTLLDC RKHLTWVVAV LQEVAAAAAQ 850
    LIAPLAENEG LLVAALEELA FKASEQIYGT PSSSPYECLR QSCNILISTM 900
    NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET 950
    VIKELKKSLK IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLEE 1000
    TQALLRKKEK EFEETMDALQ ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG 1050
    PPPSGIATLV SGIAGEEQQR GAIPGQAPGS VPGPGLVKDS PLLLQQISAM 1100
    RLHISQLQHE NSILKGAQMK ASLASLPPLH VAKLSHEGPG SELPAGALYR 1150
    KTSQLLETLN QLSTHTHVVD ITRTSPAAKS PSAQLMEQVA QLKSLSDTVE 1200
    KLKDEVLKET VSQRPGATVP TDFATFPSSA FLRAKEEQQD DTVYMGKVTF 1250
    SCAAGFGQRH RLVLTQEQLH QLHSRLIS 1278
    Length:1,278
    Mass (Da):141,695
    Last modified:October 18, 2001 - v3
    Checksum:i6DCEA5E67856E4BC
    GO
    Isoform p135 (identifier: Q14203-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-138: MAQSKRHVYS...SKLRGLKPKK → MMRQ

    Show »
    Length:1,144
    Mass (Da):127,404
    Checksum:iD4011CF3E4BF06F6
    GO
    Isoform 3 (identifier: Q14203-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-17: Missing.
         132-151: Missing.
         1066-1070: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,236
    Mass (Da):136,820
    Checksum:iF4374391D7DF0F04
    GO
    Isoform 4 (identifier: Q14203-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         132-151: Missing.
         1066-1070: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,253
    Mass (Da):138,750
    Checksum:i9871FA03C07751DE
    GO
    Isoform 5 (identifier: Q14203-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-138: MAQSKRHVYS...SKLRGLKPKK → MMRQ
         1066-1070: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,139
    Mass (Da):126,733
    Checksum:i1997342C3ADA9F82
    GO
    Isoform 6 (identifier: Q14203-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         132-138: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,271
    Mass (Da):140,887
    Checksum:i7BCE4BF282947485
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101S → N in CAA67333. (PubMed:8856662)Curated
    Sequence conflicti257 – 2571Q → R in BAG59757. (PubMed:14702039)Curated
    Sequence conflicti349 – 3491K → R in AK314352. (PubMed:14702039)Curated
    Sequence conflicti368 – 3681A → V in AK314352. (PubMed:14702039)Curated
    Sequence conflicti526 – 5261H → N in AAH71583. (PubMed:15489334)Curated
    Sequence conflicti618 – 6181K → R in AAH71583. (PubMed:15489334)Curated
    Sequence conflicti712 – 7121D → V in CAA67333. (PubMed:8856662)Curated
    Sequence conflicti1081 – 10811V → M in AAP35404. 1 PublicationCurated
    Sequence conflicti1261 – 12611R → Q in BAG59757. (PubMed:14702039)Curated
    Sequence conflicti1274 – 12741S → I in AAH71583. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti59 – 591G → S in HMN7B; shows a modestly reduced affinity for microtubules which has been suggested to impair axonal transport; the effect is identical to that of complete loss of the CAP-Gly domain. 1 Publication
    VAR_015850
    Natural varianti71 – 711G → A in PERRYS. 1 Publication
    VAR_063867
    Natural varianti71 – 711G → E in PERRYS. 1 Publication
    VAR_063868
    Natural varianti71 – 711G → R in PERRYS; diminishes microtubule binding and lead to intracytoplasmic inclusions. 1 Publication
    VAR_063869
    Natural varianti72 – 721T → P in PERRYS. 1 Publication
    VAR_063870
    Natural varianti74 – 741Q → P in PERRYS; diminishes microtubule binding and lead to intracytoplasmic inclusions. 1 Publication
    VAR_063871
    Natural varianti163 – 1631A → P.
    VAR_001373
    Natural varianti287 – 2871L → M.
    Corresponds to variant rs13420401 [ dbSNP | Ensembl ].
    VAR_048677
    Natural varianti495 – 4951R → Q.
    Corresponds to variant rs17721059 [ dbSNP | Ensembl ].
    VAR_048678
    Natural varianti571 – 5711M → T in susceptibility to amyotrophic lateral sclerosis. 1 Publication
    VAR_063872
    Natural varianti785 – 7851R → W in susceptibility to amyotrophic lateral sclerosis. 1 Publication
    VAR_063873
    Natural varianti1101 – 11011R → K in susceptibility to amyotrophic lateral sclerosis. 1 Publication
    VAR_063874
    Natural varianti1249 – 12491T → I in susceptibility to amyotrophic lateral sclerosis; unknown pathological significance. 2 Publications
    Corresponds to variant rs72466496 [ dbSNP | Ensembl ].
    VAR_063875

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 138138MAQSK…LKPKK → MMRQ in isoform p135 and isoform 5. 1 PublicationVSP_000760Add
    BLAST
    Alternative sequencei1 – 1717Missing in isoform 3. 1 PublicationVSP_045392Add
    BLAST
    Alternative sequencei132 – 15120Missing in isoform 3 and isoform 4. 1 PublicationVSP_045393Add
    BLAST
    Alternative sequencei132 – 1387Missing in isoform 6. 1 PublicationVSP_047174
    Alternative sequencei1066 – 10705Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsVSP_045394

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064205, AF064203, AF064204 Genomic DNA. Translation: AAD55811.1.
    AF064205, AF064204 Genomic DNA. Translation: AAD55812.1.
    AK297286 mRNA. Translation: BAG59757.1.
    AK314352 mRNA. No translation available.
    AC005041 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAW99684.1.
    BC071583 mRNA. Translation: AAH71583.1.
    X98801 mRNA. Translation: CAA67333.1.
    AF086947
    , AF086927, AF086928, AF086929, AF086930, AF086931, AF086932, AF086933, AF086934, AF086935, AF086936, AF086937, AF086938, AF086939, AF086940, AF086941, AF086942, AF086943, AF086944, AF086945, AF086946 Genomic DNA. Translation: AAD03694.1.
    BT006758 mRNA. Translation: AAP35404.1.
    CCDSiCCDS1939.1. [Q14203-1]
    CCDS46341.1. [Q14203-4]
    CCDS46342.1. [Q14203-5]
    CCDS46343.1. [Q14203-2]
    CCDS54368.1. [Q14203-3]
    CCDS54369.1. [Q14203-6]
    RefSeqiNP_001128512.1. NM_001135040.2. [Q14203-4]
    NP_001128513.1. NM_001135041.2. [Q14203-5]
    NP_001177765.1. NM_001190836.1. [Q14203-3]
    NP_001177766.1. NM_001190837.1. [Q14203-6]
    NP_004073.2. NM_004082.4. [Q14203-1]
    NP_075408.1. NM_023019.3. [Q14203-2]
    UniGeneiHs.516111.

    Genome annotation databases

    EnsembliENST00000361874; ENSP00000354791; ENSG00000204843. [Q14203-1]
    ENST00000394003; ENSP00000377571; ENSG00000204843. [Q14203-6]
    ENST00000409240; ENSP00000386406; ENSG00000204843. [Q14203-3]
    ENST00000409438; ENSP00000387270; ENSG00000204843. [Q14203-5]
    ENST00000409567; ENSP00000386843; ENSG00000204843. [Q14203-4]
    GeneIDi1639.
    KEGGihsa:1639.
    UCSCiuc002sku.3. human.
    uc002skv.3. human. [Q14203-2]
    uc002skx.3. human. [Q14203-1]

    Polymorphism databases

    DMDMi17375490.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064205 , AF064203 , AF064204 Genomic DNA. Translation: AAD55811.1 .
    AF064205 , AF064204 Genomic DNA. Translation: AAD55812.1 .
    AK297286 mRNA. Translation: BAG59757.1 .
    AK314352 mRNA. No translation available.
    AC005041 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAW99684.1 .
    BC071583 mRNA. Translation: AAH71583.1 .
    X98801 mRNA. Translation: CAA67333.1 .
    AF086947
    , AF086927 , AF086928 , AF086929 , AF086930 , AF086931 , AF086932 , AF086933 , AF086934 , AF086935 , AF086936 , AF086937 , AF086938 , AF086939 , AF086940 , AF086941 , AF086942 , AF086943 , AF086944 , AF086945 , AF086946 Genomic DNA. Translation: AAD03694.1 .
    BT006758 mRNA. Translation: AAP35404.1 .
    CCDSi CCDS1939.1. [Q14203-1 ]
    CCDS46341.1. [Q14203-4 ]
    CCDS46342.1. [Q14203-5 ]
    CCDS46343.1. [Q14203-2 ]
    CCDS54368.1. [Q14203-3 ]
    CCDS54369.1. [Q14203-6 ]
    RefSeqi NP_001128512.1. NM_001135040.2. [Q14203-4 ]
    NP_001128513.1. NM_001135041.2. [Q14203-5 ]
    NP_001177765.1. NM_001190836.1. [Q14203-3 ]
    NP_001177766.1. NM_001190837.1. [Q14203-6 ]
    NP_004073.2. NM_004082.4. [Q14203-1 ]
    NP_075408.1. NM_023019.3. [Q14203-2 ]
    UniGenei Hs.516111.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TXQ X-ray 1.80 A 15-107 [» ]
    2COY NMR - A 1-99 [» ]
    2HKN X-ray 1.87 A/B 18-111 [» ]
    2HKQ X-ray 1.86 B 18-111 [» ]
    2HL3 X-ray 2.03 A/B 18-111 [» ]
    2HL5 X-ray 1.93 C/D 18-111 [» ]
    2HQH X-ray 1.80 A/B/C/D 15-107 [» ]
    3E2U X-ray 2.60 A/B/C/D 18-111 [» ]
    3TQ7 X-ray 2.30 P/Q 27-97 [» ]
    ProteinModelPortali Q14203.
    SMRi Q14203. Positions 25-98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108007. 58 interactions.
    DIPi DIP-31365N.
    IntActi Q14203. 42 interactions.
    MINTi MINT-5004548.
    STRINGi 9606.ENSP00000354791.

    PTM databases

    PhosphoSitei Q14203.

    Polymorphism databases

    DMDMi 17375490.

    2D gel databases

    OGPi Q14203.

    Proteomic databases

    MaxQBi Q14203.
    PaxDbi Q14203.
    PRIDEi Q14203.

    Protocols and materials databases

    DNASUi 1639.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361874 ; ENSP00000354791 ; ENSG00000204843 . [Q14203-1 ]
    ENST00000394003 ; ENSP00000377571 ; ENSG00000204843 . [Q14203-6 ]
    ENST00000409240 ; ENSP00000386406 ; ENSG00000204843 . [Q14203-3 ]
    ENST00000409438 ; ENSP00000387270 ; ENSG00000204843 . [Q14203-5 ]
    ENST00000409567 ; ENSP00000386843 ; ENSG00000204843 . [Q14203-4 ]
    GeneIDi 1639.
    KEGGi hsa:1639.
    UCSCi uc002sku.3. human.
    uc002skv.3. human. [Q14203-2 ]
    uc002skx.3. human. [Q14203-1 ]

    Organism-specific databases

    CTDi 1639.
    GeneCardsi GC02M074588.
    GeneReviewsi DCTN1.
    H-InvDB HIX0204314.
    HGNCi HGNC:2711. DCTN1.
    HPAi CAB009108.
    HPA034635.
    MIMi 105400. phenotype.
    168605. phenotype.
    601143. gene.
    607641. phenotype.
    neXtProti NX_Q14203.
    Orphaneti 803. Amyotrophic lateral sclerosis.
    139589. Distal hereditary motor neuropathy type 7.
    178509. Perry syndrome.
    PharmGKBi PA27180.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5244.
    HOGENOMi HOG000015352.
    HOVERGENi HBG004956.
    KOi K04648.
    OMAi PEDSTMQ.
    OrthoDBi EOG79W94B.
    PhylomeDBi Q14203.
    TreeFami TF105246.

    Enzyme and pathway databases

    Reactomei REACT_121399. MHC class II antigen presentation.
    REACT_15296. Recruitment of mitotic centrosome proteins and complexes.
    REACT_15364. Loss of Nlp from mitotic centrosomes.
    REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
    REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
    REACT_18273. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    ChiTaRSi DCTN1. human.
    EvolutionaryTracei Q14203.
    GeneWikii DCTN1.
    GenomeRNAii 1639.
    NextBioi 6734.
    PMAP-CutDB Q14203.
    PROi Q14203.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14203.
    Bgeei Q14203.
    CleanExi HS_DCTN1.
    Genevestigatori Q14203.

    Family and domain databases

    Gene3Di 2.30.30.190. 1 hit.
    InterProi IPR000938. CAP-Gly_domain.
    IPR027663. DCTN1.
    IPR022157. Dynactin.
    [Graphical view ]
    PANTHERi PTHR18916:SF29. PTHR18916:SF29. 1 hit.
    Pfami PF01302. CAP_GLY. 1 hit.
    PF12455. Dynactin. 1 hit.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74924. SSF74924. 1 hit.
    PROSITEi PS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome."
      Collin G.B., Nishina P.M., Marshall J.D., Naggert J.K.
      Genomics 53:359-364(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
      Tissue: Brain and Trachea.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
      Tissue: Brain.
    6. "Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization."
      Holzbaur E.L.F., Tokito M.K.
      Genomics 31:398-399(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-1278.
      Tissue: Brain.
    7. "Functionally distinct isoforms of dynactin are expressed in human neurons."
      Tokito M.K., Howland D.S., Lee V.M.-Y., Holzbaur E.L.F.
      Mol. Biol. Cell 7:1167-1180(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-1278 (ISOFORM 6), ALTERNATIVE SPLICING.
      Tissue: Brain.
    8. "The genomic structure of DCTN1, a candidate gene for limb-girdle muscular dystrophy."
      Tokito M.K., Holzbaur E.L.F.
      Biochim. Biophys. Acta 1442:432-436(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-1278.
    9. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1081-1278.
    10. "Characterization of functional domains of human EB1 family proteins."
      Bu W., Su L.-K.
      J. Biol. Chem. 278:49721-49731(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPRE1; MAPRE2 AND MAPRE3.
    11. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "FBXL5 interacts with p150Glued and regulates its ubiquitination."
      Zhang N., Liu J., Ding X., Aikhionbare F., Jin C., Yao X.
      Biochem. Biophys. Res. Commun. 359:34-39(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION, INTERACTION WITH FBXL5.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. "The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport."
      Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J.
      Cell Res. 19:1334-1349(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX6.
    15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-108, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    16. "A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components."
      Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M.
      J. Biol. Chem. 285:31616-31633(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ECM29.
    17. "A novel human dynactin-associated protein, dynAP, promotes activation of Akt, and ergosterol-related compounds induce dynAP-dependent apoptosis of human cancer cells."
      Kunoh T., Noda T., Koseki K., Sekigawa M., Takagi M., Shin-ya K., Goshima N., Iemura S., Natsume T., Wada S., Mukai Y., Ohta S., Sasaki R., Mizukami T.
      Mol. Cancer Ther. 9:2934-2942(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DYNAP.
    18. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. "Neuronal ceroid lipofuscinosis protein CLN3 interacts with motor proteins and modifies location of late endosomal compartments."
      Uusi-Rauva K., Kyttala A., van der Kant R., Vesa J., Tanhuanpaa K., Neefjes J., Olkkonen V.M., Jalanko A.
      Cell. Mol. Life Sci. 69:2075-2089(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLN3.
    20. Cited for: INTERACTION WITH CEP131.
    21. "MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression."
      Zhu M., Settele F., Kotak S., Sanchez-Pulido L., Ehret L., Ponting C.P., Goenczy P., Hoffmann I.
      J. Cell Biol. 200:773-787(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MISP.
    22. "Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex."
      Hayashi I., Wilde A., Mal T.K., Ikura M.
      Mol. Cell 19:449-460(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 15-107 IN COMPLEX WITH MAPRE1, INTERACTION WITH MAPRE1.
    23. "Solution structure of the CAP-Gly domain in human dynactin 1."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 1-99.
    24. Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 18-111 IN COMPLEX WITH MAPRE1, INTERACTION WITH MAPRE1.
    25. Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 15-111 IN COMPLEX WITH CLIP1, SUBCELLULAR LOCATION, INTERACTION WITH CLIP1.
    26. "CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1."
      Hayashi I., Plevin M.J., Ikura M.
      Nat. Struct. Mol. Biol. 14:980-981(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 15-107 IN COMPLEX WITH CLIP1, INTERACTION WITH CLIP1, MUTAGENESIS OF LYS-68 AND ARG-90.
    27. Cited for: VARIANT HMN7B SER-59.
    28. Cited for: VARIANTS SUSCEPTIBILITY TO ALS THR-571; TRP-785 AND ILE-1249.
    29. Cited for: VARIANT SUSCEPTIBILITY TO ALS LYS-1101.
    30. "A motor neuron disease-associated mutation in p150Glued perturbs dynactin function and induces protein aggregation."
      Levy J.R., Sumner C.J., Caviston J.P., Tokito M.K., Ranganathan S., Ligon L.A., Wallace K.E., LaMonte B.H., Harmison G.G., Puls I., Fischbeck K.H., Holzbaur E.L.F.
      J. Cell Biol. 172:733-745(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF VARIANT HMN7B SER-59.
    31. Cited for: VARIANTS PERRYS ARG-71; GLU-71; ALA-71; PRO-72 AND PRO-74, CHARACTERIZATION OF VARIANTS PERRYS ARG-71 AND PRO-74, CHARACTERIZATION OF VARIANT HMN7B SER-59.
    32. Cited for: VARIANT ILE-1249.
    33. "Neurodegeneration mutations in dynactin impair dynein-dependent nuclear migration."
      Moore J.K., Sept D., Cooper J.A.
      Proc. Natl. Acad. Sci. U.S.A. 106:5147-5152(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF VARIANT HMN7B SER-59.

    Entry informationi

    Entry nameiDCTN1_HUMAN
    AccessioniPrimary (citable) accession number: Q14203
    Secondary accession number(s): A8MY36
    , B4DM45, E9PFS5, E9PGE1, G5E9H4, O95296, Q6IQ37, Q9BRM9, Q9UIU1, Q9UIU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 146 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3