Reviewed, UniProtKB/Swiss-Prot Q141F5 (HUTI_BURXL)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Bxeno_A1496
ORF Names:	Bxe_A2943

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Imidazolonepropionase HAMAP MF_00372		PRO_0000306455
Sites
	Metal binding	67	1	Zinc or iron By similarity
	Metal binding	69	1	Zinc or iron By similarity
	Metal binding	237	1	Zinc or iron By similarity
	Metal binding	312	1	Zinc or iron By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	240	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q141F5-1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 3E312BA841FB71B6	FASTA	411	44,612
	10 20 30 40 50 60 MKQTVWHHLK LCPQGDPQHT LADAAIAVEN GTIVWLGAAC ALPVEYAAWP REDLHGAWVT 70 80 90 100 110 120 PGLVDCHTHL VYGGQRADEF AQRLAGVSYE EIARQGGGIV STVRATRAAD EASLLRQSAA 130 140 150 160 170 180 RLEPLLAEGV TTVEIKSGYG LDLASERKML RVARQLGERY PVTVYTTFLG AHALPPEFTG 190 200 210 220 230 240 RADAYIDEVC NTMLPALADE GLVDAVDVFC ERIGFSLEQS ERVFKAAERY QLPVKMHAEQ 250 260 270 280 290 300 LSNGGGTALA ARHHALSADH LEFLDEAGVA AMKEAGTVAV LLPGAYYFIR ETQLPPLELL 310 320 330 340 350 360 RRYQVPIAIS TDSNPGTSPA TSLLLMMNMA TTLFRMTVPE VLQGVTSHAA RALGKADRHG 370 380 390 400 410 SLEAGRAADF AVWSVDSLAE LAYWIGRPLC ARVVRAGETV YTRRELSQGL R

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE30034.1. Different initiation.
RefSeq	YP_558086.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4003963.
GenomeReviews	Gene locus Bxeno_A1496 in contig CP000270_GR.
KEGG	bxe:Bxe_A2943.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q141F5.
OMA	ECHTHAI.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURXL

Accession

Primary (citable) accession number: Q141F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)