Reviewed, UniProtKB/Swiss-Prot Q141D3 (ASTD_BURXL)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	Bxeno_A1518
ORF Names:	Bxe_A2920

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_0000262393
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q141D3-1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: B6578808C1255D64	FASTA	487	51,705
	10 20 30 40 50 60 MSELFIGGEW AAGTGPAFAS RNPGTGAAVW EGNSASADDV DRAVRSARRA FAAWSASSVD 70 80 90 100 110 120 ERCAVVRRFA ALVTERKEAL AEAIGRETGK PLWEARTEAA SMAAKVEISI QAYNERTGEK 130 140 150 160 170 180 RSAMADGTAV LRHRPHGVVA VFGPYNFPGH LPNGHIVPAL IAGNAVVFKP SELAPGVAAL 190 200 210 220 230 240 TVQIWRDAGL PAGVLNLVQG EKDTGIALAN HRQIDGLFFT GSSDTGTLLH KQFGGRPEIV 250 260 270 280 290 300 LALEMGGNNP LVIGPVADVD AAVHHTIQSA FLSAGQRCTC ARRIFVPNDA AGDRFLERFT 310 320 330 340 350 360 EVTSRITVGE YNADPQPFMG AVISVRAASR LVAAQERLLA DGAKALLKME QRDPKLGFVT 370 380 390 400 410 420 PAILDVTNVA NRPDEEHFGP LAQIIRYGSF NEALEQANDT EFGLSAGLLA DDEALWAHFQ 430 440 450 460 470 480 RTIRAGIVNW NRPTNGASSG APFGGPGRSG NHRPSAYYAA DYCAFPMASV ESAQLQMPAS VSPGLQF

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE30056.1. Different initiation.
RefSeq	YP_558108.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4004449.
GenomeReviews	Gene locus Bxeno_A1518 in contig CP000270_GR.
KEGG	bxe:Bxe_A2920.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q141D3.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_BURXL

Accession

Primary (citable) accession number: Q141D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	November 25, 2008
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)