Reviewed, UniProtKB/Swiss-Prot Q141D2 (ASTB_BURXL)

Names and origin

Protein names

Recommended name:
    N-succinylarginine dihydrolase
    EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	Bxeno_A1519
ORF Names:	Bxe_A2919

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity.
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000262344
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	249	1	By similarity
	Active site	370	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	364	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q141D2-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 3FF0ECB529441E9A	FASTA	446	48,896
	10 20 30 40 50 60 MQATEANFDG LVGPTHNYAG LSFGNVASQN NEKSVANPKA AAKQGLRKMK QLADLGFHQG 70 80 90 100 110 120 VLPPQERPSM RLLRELGFSG DDATVIARVA RDAPELLAAA SSASAMWTAN AATVSPSADT 130 140 150 160 170 180 NDGRVHFTPA NLCSKLHRAI EHESTRRTLR TMFNDTDRFV VHEALPGTPA LGDEGAANHT 190 200 210 220 230 240 RFCEEYGARG VEFFVYGRSE YRRGPEPKRF PARQSFEASR AVAHRHGLAD EATVYAQQNP 250 260 270 280 290 300 DVIDAGVFHN DVIAVGNRNT LFCHQFAFVE PNAVYDELRA KLSGLKAAFN VIEVPDAQVS 310 320 330 340 350 360 VADAVSSYLF NSQLLTRPDG KQVLVVPQEC RENPRVAAYL DDLTSRTGPI DDVLVFDLRE 370 380 390 400 410 420 SMKNGGGPAC LRLRVVLDDA ERAAVTPGVW IDDTLFGRLD AWIEKHYRDR LAPADLTDPH 430 440 LLAESRTALD ELTQILGLGS LYDFQR

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE30057.1.
RefSeq	YP_558109.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4004450.
GenomeReviews	Gene locus Bxeno_A1519 in contig CP000270_GR.
KEGG	bxe:Bxe_A2919.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q141D2.
OMA	Q141D2. HFAHHPA.
Family and domain databases
HAMAP	MF_01172. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_BURXL

Accession

Primary (citable) accession number: Q141D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)