Reviewed, UniProtKB/Swiss-Prot Q141D1 (ASTE_BURXL)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Bxeno_A1520
ORF Names:	Bxe_A2918

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000257710
Sites
	Active site	222	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q141D1-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: FE230AF81640883E	FASTA	342	37,411
	10 20 30 40 50 60 MPVSLLDDFL AYTLAGTRPA ASEAQGTCAG GVRWSWLDDG VLLMEPAVQE EGMRSVLVSA 70 80 90 100 110 120 GVHGDETAPI ELLAFLVRDI AHGTAALTGR LLVILGNVDA MRDACRYRDD DLNRLFSGRH 130 140 150 160 170 180 LQLPQSHEAP RAAALERAAT RFFAAAPDNP GARWHIDMHT AIRASAFEQF ALLPHTGKPF 190 200 210 220 230 240 SRAMFEWLGE ARISAVLLHT TRGNTYSHFT AQACGALACT LELGKVRPFG QNDLTRFAGA 250 260 270 280 290 300 DHAVRHLLAG MRGEVRAPMP RAFTVIDQIT RQSEAFELLV APDVANFTPF AKDTVLARDG 310 320 330 340 DYRYVVRHDE ERLVFPNATV KPGLRAGLMV IETTQDTLSK LV

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE30058.1.
RefSeq	YP_558110.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4004451.
GenomeReviews	Gene locus Bxeno_A1520 in contig CP000270_GR.
KEGG	bxe:Bxe_A2918.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q141D1.
OMA	Q141D1. EKFAIYP.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_BURXL

Accession

Primary (citable) accession number: Q141D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)