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Q14197 (ICT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidyl-tRNA hydrolase ICT1, mitochondrial

EC=3.1.1.29
Alternative name(s):
39S ribosomal protein L58, mitochondrial
Short name=MRP-L58
Digestion substraction 1
Short name=DS-1
Immature colon carcinoma transcript 1 protein
Gene names
Name:ICT1
Synonyms:DS1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Ref.7

Catalytic activity

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

Subunit structure

Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. Ref.7 Ref.9

Subcellular location

Mitochondrion Ref.7 Ref.9.

Tissue specificity

Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells. Ref.1

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.

Caution

In contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site and determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   Coding sequence diversityPolymorphism
   DomainTransit peptide
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitochondrial translational termination

Inferred from direct assay Ref.7. Source: UniProtKB

   Cellular_componentmitochondrial large ribosomal subunit

Inferred from direct assay Ref.7. Source: UniProtKB

mitochondrion

Inferred from direct assay Ref.7. Source: UniProtKB

   Molecular_functionaminoacyl-tRNA hydrolase activity

Inferred from direct assay Ref.7. Source: UniProtKB

translation release factor activity, codon nonspecific

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2929Mitochondrion Potential
Chain30 – 206177Peptidyl-tRNA hydrolase ICT1, mitochondrial
PRO_0000030339

Natural variations

Natural variant81R → P.
Corresponds to variant rs3744206 [ dbSNP | Ensembl ].
VAR_020045
Natural variant771L → F.
Corresponds to variant rs10512599 [ dbSNP | Ensembl ].
VAR_024604
Natural variant1221T → M.
Corresponds to variant rs34496172 [ dbSNP | Ensembl ].
VAR_061767

Experimental info

Mutagenesis881G → A: Strongly impairs peptide release activity. Ref.7
Mutagenesis891G → S: Strongly impairs peptide release activity. Ref.7
Sequence conflict941K → R in BAD96273. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q14197 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 663BF52443D41540

FASTA20623,630
        10         20         30         40         50         60 
MAATRCLRWG LSRAGVWLLP PPARCPRRAL HKQKDGTEFK SIYSLDKLYP ESQGSDTAWR 

        70         80         90        100        110        120 
VPNGAKQADS DIPLDRLTIS YCRSSGPGGQ NVNKVNSKAE VRFHLATAEW IAEPVRQKIA 

       130        140        150        160        170        180 
ITHKNKINRL GELILTSESS RYQFRNLADC LQKIRDMITE ASQTPKEPTK EDVKLHRIRI 

       190        200 
ENMNRERLRQ KRIHSAVKTS RRVDMD 

« Hide

References

« Hide 'large scale' references
[1]"Identification of mRNAs that show modulated expression during colon carcinoma cell differentiation."
van Belzen N., Diesveld M.P.G., van der Made A.C.J., Nozawa Y., Dinjens W.N.M., Vlietstra R., Trapman J., Bosman F.T.
Eur. J. Biochem. 234:843-848(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Tongue.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Adipose tissue.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph.
[7]"A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome."
Richter R., Rorbach J., Pajak A., Smith P.M., Wessels H.J., Huynen M.A., Smeitink J.A., Lightowlers R.N., Chrzanowska-Lightowlers Z.M.
EMBO J. 29:1116-1125(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE MITOCHONDRIAL 39S RIBOSOMAL SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-88 AND GLY-89.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome."
Koc E.C., Cimen H., Kumcuoglu B., Abu N., Akpinar G., Haque M.E., Spremulli L.L., Koc H.
Front. Physiol. 4:183-183(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81788 mRNA. Translation: CAA57387.1.
BT007111 mRNA. Translation: AAP35775.1.
AK222553 mRNA. Translation: BAD96273.1.
AK314138 mRNA. Translation: BAG36828.1.
CH471099 Genomic DNA. Translation: EAW89227.1.
BC015335 mRNA. Translation: AAH15335.1.
CCDSCCDS11711.1.
PIRS63540.
RefSeqNP_001536.1. NM_001545.1.
UniGeneHs.407955.

3D structure databases

ProteinModelPortalQ14197.
SMRQ14197. Positions 65-162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109622. 236 interactions.
IntActQ14197. 217 interactions.
MINTMINT-3029229.
STRING9606.ENSP00000301585.

PTM databases

PhosphoSiteQ14197.

Proteomic databases

MaxQBQ14197.
PaxDbQ14197.
PRIDEQ14197.

Protocols and materials databases

DNASU3396.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301585; ENSP00000301585; ENSG00000167862.
GeneID3396.
KEGGhsa:3396.
UCSCuc002jmm.3. human.

Organism-specific databases

CTD3396.
GeneCardsGC17P073008.
HGNCHGNC:5359. ICT1.
HPAHPA003634.
MIM603000. gene.
neXtProtNX_Q14197.
PharmGKBPA29607.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1186.
HOGENOMHOG000231063.
HOVERGENHBG006115.
InParanoidQ14197.
KOK15033.
OMALYPESRG.
PhylomeDBQ14197.
TreeFamTF315161.

Gene expression databases

ArrayExpressQ14197.
BgeeQ14197.
CleanExHS_ICT1.
GenevestigatorQ14197.

Family and domain databases

InterProIPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamPF00472. RF-1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi3396.
NextBio13418.
PROQ14197.
SOURCESearch...

Entry information

Entry nameICT1_HUMAN
AccessionPrimary (citable) accession number: Q14197
Secondary accession number(s): B2RAD1, Q53HM7, Q53Y11
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM