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Protein

Peptidyl-tRNA hydrolase ICT1, mitochondrial

Gene

MRPL58

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.1 Publication

Caution

In contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site and determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity.Curated

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

  • aminoacyl-tRNA hydrolase activity Source: UniProtKB
  • translation release factor activity, codon nonspecific Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ribonucleoprotein, Ribosomal protein
Biological processProtein biosynthesis

Enzyme and pathway databases

BRENDAi3.1.1.29 2681
ReactomeiR-HSA-5368286 Mitochondrial translation initiation
R-HSA-5389840 Mitochondrial translation elongation
R-HSA-5419276 Mitochondrial translation termination

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase ICT1, mitochondrial (EC:3.1.1.29)
Alternative name(s):
39S ribosomal protein L58, mitochondrialImported
Short name:
MRP-L58
Digestion substraction 1
Short name:
DS-1
Immature colon carcinoma transcript 1 protein
Mitochondrial large ribosomal subunit protein mL621 Publication
Gene namesi
Name:MRPL58Imported
Synonyms:DS1, ICT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000167862.9
HGNCiHGNC:5359 MRPL58
MIMi603000 gene
neXtProtiNX_Q14197

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88G → A: Strongly impairs peptide release activity. 1 Publication1
Mutagenesisi89G → S: Strongly impairs peptide release activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000167862
PharmGKBiPA29607

Polymorphism and mutation databases

BioMutaiICT1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000003033930 – 206Peptidyl-tRNA hydrolase ICT1, mitochondrialAdd BLAST177

Proteomic databases

EPDiQ14197
MaxQBiQ14197
PaxDbiQ14197
PeptideAtlasiQ14197
PRIDEiQ14197
ProteomicsDBi59919
TopDownProteomicsiQ14197

PTM databases

iPTMnetiQ14197
PhosphoSitePlusiQ14197

Expressioni

Tissue specificityi

Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.1 Publication

Gene expression databases

BgeeiENSG00000167862
CleanExiHS_ICT1
ExpressionAtlasiQ14197 baseline and differential
GenevisibleiQ14197 HS

Organism-specific databases

HPAiHPA003634

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.4 Publications

Protein-protein interaction databases

BioGridi109622, 248 interactors
IntActiQ14197, 222 interactors
MINTiQ14197
STRINGi9606.ENSP00000301585

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 48Combined sources4
Helixi55 – 58Combined sources4
Turni74 – 76Combined sources3
Beta strandi77 – 81Combined sources5
Beta strandi99 – 107Combined sources9
Helixi113 – 122Combined sources10
Turni123 – 125Combined sources3
Beta strandi131 – 137Combined sources7
Beta strandi140 – 142Combined sources3
Helixi143 – 162Combined sources20
Helixi175 – 188Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40p1-206[»]
3J9Melectron microscopy3.50p1-206[»]
5OOLelectron microscopy3.06p1-206[»]
5OOMelectron microscopy3.03p1-206[»]
ProteinModelPortaliQ14197
SMRiQ14197
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3429 Eukaryota
COG1186 LUCA
GeneTreeiENSGT00390000013268
HOGENOMiHOG000231063
HOVERGENiHBG006115
InParanoidiQ14197
KOiK15033
OMAiKIYPNAR
OrthoDBiEOG091G0SBR
PhylomeDBiQ14197
TreeFamiTF315161

Family and domain databases

InterProiView protein in InterPro
IPR000352 Pep_chain_release_fac_I_II
PfamiView protein in Pfam
PF00472 RF-1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q14197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATRCLRWG LSRAGVWLLP PPARCPRRAL HKQKDGTEFK SIYSLDKLYP
60 70 80 90 100
ESQGSDTAWR VPNGAKQADS DIPLDRLTIS YCRSSGPGGQ NVNKVNSKAE
110 120 130 140 150
VRFHLATAEW IAEPVRQKIA ITHKNKINRL GELILTSESS RYQFRNLADC
160 170 180 190 200
LQKIRDMITE ASQTPKEPTK EDVKLHRIRI ENMNRERLRQ KRIHSAVKTS

RRVDMD
Length:206
Mass (Da):23,630
Last modified:November 1, 1996 - v1
Checksum:i663BF52443D41540
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94K → R in BAD96273 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0200458R → P. Corresponds to variant dbSNP:rs3744206Ensembl.1
Natural variantiVAR_02460477L → F. Corresponds to variant dbSNP:rs10512599Ensembl.1
Natural variantiVAR_061767122T → M. Corresponds to variant dbSNP:rs34496172Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81788 mRNA Translation: CAA57387.1
BT007111 mRNA Translation: AAP35775.1
AK222553 mRNA Translation: BAD96273.1
AK314138 mRNA Translation: BAG36828.1
CH471099 Genomic DNA Translation: EAW89227.1
BC015335 mRNA Translation: AAH15335.1
CCDSiCCDS11711.1
PIRiS63540
RefSeqiNP_001290194.1, NM_001303265.1
NP_001536.1, NM_001545.2
UniGeneiHs.407955

Genome annotation databases

EnsembliENST00000301585; ENSP00000301585; ENSG00000167862
GeneIDi3396
KEGGihsa:3396
UCSCiuc002jmm.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiICT1_HUMAN
AccessioniPrimary (citable) accession number: Q14197
Secondary accession number(s): B2RAD1, Q53HM7, Q53Y11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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