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Protein

Peptidyl-tRNA hydrolase ICT1, mitochondrial

Gene

MRPL58

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.1 Publication

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

  • aminoacyl-tRNA hydrolase activity Source: UniProtKB
  • ribosome binding Source: GO_Central
  • translation release factor activity, codon nonspecific Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167862-MONOMER.
BRENDAi3.1.1.29. 2681.
ReactomeiR-HSA-5368286. Mitochondrial translation initiation.
R-HSA-5389840. Mitochondrial translation elongation.
R-HSA-5419276. Mitochondrial translation termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase ICT1, mitochondrial (EC:3.1.1.29)
Alternative name(s):
39S ribosomal protein L58, mitochondrialImported
Short name:
MRP-L58
Digestion substraction 1
Short name:
DS-1
Immature colon carcinoma transcript 1 protein
Gene namesi
Name:MRPL58Imported
Synonyms:DS1, ICT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:5359. MRPL58.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: Reactome
  • mitochondrial large ribosomal subunit Source: UniProtKB
  • mitochondrial matrix Source: Reactome
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88G → A: Strongly impairs peptide release activity. 1 Publication1
Mutagenesisi89G → S: Strongly impairs peptide release activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000167862.
PharmGKBiPA29607.

Polymorphism and mutation databases

BioMutaiICT1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000003033930 – 206Peptidyl-tRNA hydrolase ICT1, mitochondrialAdd BLAST177

Proteomic databases

EPDiQ14197.
MaxQBiQ14197.
PaxDbiQ14197.
PeptideAtlasiQ14197.
PRIDEiQ14197.
TopDownProteomicsiQ14197.

PTM databases

iPTMnetiQ14197.
PhosphoSitePlusiQ14197.

Expressioni

Tissue specificityi

Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.1 Publication

Gene expression databases

BgeeiENSG00000167862.
CleanExiHS_ICT1.
ExpressionAtlasiQ14197. baseline and differential.
GenevisibleiQ14197. HS.

Organism-specific databases

HPAiHPA003634.

Interactioni

Subunit structurei

Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.2 Publications

Protein-protein interaction databases

BioGridi109622. 243 interactors.
IntActiQ14197. 218 interactors.
MINTiMINT-3029229.
STRINGi9606.ENSP00000301585.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40p1-206[»]
3J9Melectron microscopy3.50p1-206[»]
ProteinModelPortaliQ14197.
SMRiQ14197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3429. Eukaryota.
COG1186. LUCA.
GeneTreeiENSGT00390000013268.
HOGENOMiHOG000231063.
HOVERGENiHBG006115.
InParanoidiQ14197.
KOiK15033.
OMAiKIYPNAR.
OrthoDBiEOG091G0SBR.
PhylomeDBiQ14197.
TreeFamiTF315161.

Family and domain databases

InterProiIPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF00472. RF-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q14197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATRCLRWG LSRAGVWLLP PPARCPRRAL HKQKDGTEFK SIYSLDKLYP
60 70 80 90 100
ESQGSDTAWR VPNGAKQADS DIPLDRLTIS YCRSSGPGGQ NVNKVNSKAE
110 120 130 140 150
VRFHLATAEW IAEPVRQKIA ITHKNKINRL GELILTSESS RYQFRNLADC
160 170 180 190 200
LQKIRDMITE ASQTPKEPTK EDVKLHRIRI ENMNRERLRQ KRIHSAVKTS

RRVDMD
Length:206
Mass (Da):23,630
Last modified:November 1, 1996 - v1
Checksum:i663BF52443D41540
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94K → R in BAD96273 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0200458R → P.Corresponds to variant rs3744206dbSNPEnsembl.1
Natural variantiVAR_02460477L → F.Corresponds to variant rs10512599dbSNPEnsembl.1
Natural variantiVAR_061767122T → M.Corresponds to variant rs34496172dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81788 mRNA. Translation: CAA57387.1.
BT007111 mRNA. Translation: AAP35775.1.
AK222553 mRNA. Translation: BAD96273.1.
AK314138 mRNA. Translation: BAG36828.1.
CH471099 Genomic DNA. Translation: EAW89227.1.
BC015335 mRNA. Translation: AAH15335.1.
CCDSiCCDS11711.1.
PIRiS63540.
RefSeqiNP_001290194.1. NM_001303265.1.
NP_001536.1. NM_001545.2.
UniGeneiHs.407955.

Genome annotation databases

EnsembliENST00000301585; ENSP00000301585; ENSG00000167862.
GeneIDi3396.
KEGGihsa:3396.
UCSCiuc002jmm.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81788 mRNA. Translation: CAA57387.1.
BT007111 mRNA. Translation: AAP35775.1.
AK222553 mRNA. Translation: BAD96273.1.
AK314138 mRNA. Translation: BAG36828.1.
CH471099 Genomic DNA. Translation: EAW89227.1.
BC015335 mRNA. Translation: AAH15335.1.
CCDSiCCDS11711.1.
PIRiS63540.
RefSeqiNP_001290194.1. NM_001303265.1.
NP_001536.1. NM_001545.2.
UniGeneiHs.407955.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40p1-206[»]
3J9Melectron microscopy3.50p1-206[»]
ProteinModelPortaliQ14197.
SMRiQ14197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109622. 243 interactors.
IntActiQ14197. 218 interactors.
MINTiMINT-3029229.
STRINGi9606.ENSP00000301585.

PTM databases

iPTMnetiQ14197.
PhosphoSitePlusiQ14197.

Polymorphism and mutation databases

BioMutaiICT1.

Proteomic databases

EPDiQ14197.
MaxQBiQ14197.
PaxDbiQ14197.
PeptideAtlasiQ14197.
PRIDEiQ14197.
TopDownProteomicsiQ14197.

Protocols and materials databases

DNASUi3396.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301585; ENSP00000301585; ENSG00000167862.
GeneIDi3396.
KEGGihsa:3396.
UCSCiuc002jmm.4. human.

Organism-specific databases

CTDi3396.
GeneCardsiICT1.
HGNCiHGNC:5359. MRPL58.
HPAiHPA003634.
MIMi603000. gene.
neXtProtiNX_Q14197.
OpenTargetsiENSG00000167862.
PharmGKBiPA29607.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3429. Eukaryota.
COG1186. LUCA.
GeneTreeiENSGT00390000013268.
HOGENOMiHOG000231063.
HOVERGENiHBG006115.
InParanoidiQ14197.
KOiK15033.
OMAiKIYPNAR.
OrthoDBiEOG091G0SBR.
PhylomeDBiQ14197.
TreeFamiTF315161.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167862-MONOMER.
BRENDAi3.1.1.29. 2681.
ReactomeiR-HSA-5368286. Mitochondrial translation initiation.
R-HSA-5389840. Mitochondrial translation elongation.
R-HSA-5419276. Mitochondrial translation termination.

Miscellaneous databases

GenomeRNAii3396.
PROiQ14197.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167862.
CleanExiHS_ICT1.
ExpressionAtlasiQ14197. baseline and differential.
GenevisibleiQ14197. HS.

Family and domain databases

InterProiIPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF00472. RF-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICT1_HUMAN
AccessioniPrimary (citable) accession number: Q14197
Secondary accession number(s): B2RAD1, Q53HM7, Q53Y11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site and determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.