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Q14195

- DPYL3_HUMAN

UniProt

Q14195 - DPYL3_HUMAN

Protein

Dihydropyrimidinase-related protein 3

Gene

DPYSL3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration By similarity.By similarity

    GO - Molecular functioni

    1. chondroitin sulfate binding Source: UniProtKB
    2. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides Source: InterPro
    3. SH3 domain binding Source: UniProtKB

    GO - Biological processi

    1. actin crosslink formation Source: UniProtKB
    2. actin filament bundle assembly Source: UniProtKB
    3. axon guidance Source: Reactome
    4. cellular response to cytokine stimulus Source: UniProtKB
    5. negative regulation of cell migration Source: UniProtKB
    6. negative regulation of neuron projection development Source: UniProtKB
    7. positive regulation of filopodium assembly Source: UniProtKB
    8. positive regulation of neuron projection development Source: UniProtKB
    9. protein homooligomerization Source: UniProtKB
    10. pyrimidine nucleobase catabolic process Source: InterPro
    11. response to axon injury Source: UniProtKB

    Enzyme and pathway databases

    ReactomeiREACT_19199. CRMPs in Sema3A signaling.

    Protein family/group databases

    MEROPSiM38.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropyrimidinase-related protein 3
    Short name:
    DRP-3
    Alternative name(s):
    Collapsin response mediator protein 4
    Short name:
    CRMP-4
    Unc-33-like phosphoprotein 1
    Short name:
    ULIP-1
    Gene namesi
    Name:DPYSL3
    Synonyms:CRMP4, DRP3, ULIP, ULIP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:3015. DPYSL3.

    Subcellular locationi

    Cytoplasm By similarity. Cell projectiongrowth cone By similarity
    Note: Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.By similarity

    GO - Cellular componenti

    1. cell body Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. extracellular space Source: UniProtKB
    4. filamentous actin Source: UniProtKB
    5. growth cone Source: UniProtKB
    6. lamellipodium Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    Orphaneti803. Amyotrophic lateral sclerosis.
    PharmGKBiPA27473.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Dihydropyrimidinase-related protein 3PRO_0000165917Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei259 – 2591Phosphoserine1 Publication
    Modified residuei431 – 4311PhosphotyrosineBy similarity
    Modified residuei509 – 5091Phosphothreonine; by GSK32 Publications
    Modified residuei514 – 5141Phosphothreonine; by GSK32 Publications
    Modified residuei518 – 5181Phosphoserine; by GSK31 Publication
    Modified residuei522 – 5221Phosphoserine; by DYRK22 Publications

    Post-translational modificationi

    Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ14195.
    PaxDbiQ14195.
    PRIDEiQ14195.

    2D gel databases

    REPRODUCTION-2DPAGEQ14195.
    UCD-2DPAGEQ14195.

    PTM databases

    PhosphoSiteiQ14195.

    Expressioni

    Tissue specificityi

    Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

    Gene expression databases

    ArrayExpressiQ14195.
    BgeeiQ14195.
    CleanExiHS_DPYSL3.
    GenevestigatoriQ14195.

    Organism-specific databases

    HPAiHPA010948.

    Interactioni

    Subunit structurei

    Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5. Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin.By similarity

    Protein-protein interaction databases

    BioGridi108143. 15 interactions.
    IntActiQ14195. 2 interactions.
    STRINGi9606.ENSP00000381526.

    Structurei

    Secondary structure

    1
    570
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi17 – 259
    Beta strandi30 – 389
    Beta strandi41 – 488
    Beta strandi56 – 594
    Beta strandi64 – 674
    Beta strandi69 – 746
    Helixi89 – 9810
    Beta strandi101 – 1088
    Helixi116 – 13015
    Beta strandi132 – 14110
    Helixi146 – 15813
    Beta strandi163 – 1686
    Turni171 – 1744
    Helixi178 – 19114
    Beta strandi194 – 1985
    Helixi202 – 21413
    Helixi221 – 2255
    Helixi229 – 24517
    Beta strandi250 – 2556
    Helixi258 – 27013
    Beta strandi274 – 2796
    Helixi280 – 2845
    Helixi287 – 2915
    Helixi295 – 3006
    Helixi313 – 32210
    Helixi338 – 3414
    Helixi342 – 3443
    Helixi348 – 3503
    Turni358 – 3603
    Helixi361 – 3699
    Turni370 – 3734
    Helixi377 – 3848
    Helixi386 – 3916
    Turni395 – 3973
    Beta strandi409 – 41911
    Turni422 – 4243
    Beta strandi426 – 4283
    Turni433 – 4364
    Beta strandi438 – 44811
    Beta strandi451 – 4555
    Helixi476 – 48712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BKNX-ray2.10A/B1-570[»]
    4CNSX-ray2.40A/B/C/D13-490[»]
    4CNUX-ray2.80A/B1-570[»]
    ProteinModelPortaliQ14195.
    SMRiQ14195. Positions 13-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0044.
    HOGENOMiHOG000219145.
    HOVERGENiHBG000806.
    OMAiTPRSSQG.
    OrthoDBiEOG7SJD48.
    PhylomeDBiQ14195.
    TreeFamiTF314706.

    Family and domain databases

    Gene3Di2.30.40.10. 2 hits.
    InterProiIPR006680. Amidohydro_1.
    IPR011778. Hydantoinase/dihydroPyrase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 2 hits.
    TIGRFAMsiTIGR02033. D-hydantoinase. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14195-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI    50
    VPGGVKTIEA NGKMVIPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG 100
    TTMIIDHVVP EPESSLTEAY EKWREWADGK SCCDYALHVD ITHWNDSVKQ 150
    EVQNLIKDKG VNSFMVYMAY KDLYQVSNTE LYEIFTCLGE LGAIAQVHAE 200
    NGDIIAQEQT RMLEMGITGP EGHVLSRPEE LEAEAVFRAI TIASQTNCPL 250
    YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA 300
    FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA 350
    IPEGTNGVEE RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR 400
    ISVGSDSDLV IWDPDAVKIV SAKNHQSAAE YNIFEGMELR GAPLVVICQG 450
    KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK RIKARRKMAD LHAVPRGMYD 500
    GPVFDLTTTP KGGTPAGSAR GSPTRPNPPV RNLHQSGFSL SGTQVDEGVR 550
    SASKRIVAPP GGRSNITSLS 570
    Length:570
    Mass (Da):61,963
    Last modified:November 1, 1996 - v1
    Checksum:i9D6AFE86CBB33AD5
    GO
    Isoform LCRMP-4 (identifier: Q14195-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-13: MSYQGKKNIPRIT → MASGRRGWDS...VLQNLGPKDK

    Note: Contains a phosphoserine at position 102.

    Show »
    Length:684
    Mass (Da):73,911
    Checksum:i1729A3ED4DA83D30
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491L → V in CAA69153. (PubMed:9115293)Curated
    Sequence conflicti142 – 1421T → A in CAA69153. (PubMed:9115293)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti442 – 4421A → S.
    Corresponds to variant rs2304044 [ dbSNP | Ensembl ].
    VAR_020485

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1313MSYQG…IPRIT → MASGRRGWDSSHEDDLPVYL ARPGTTDQVPRQKYGGMFCN VEGAFESKTLDFDALSVGQR GAKTPRSGQGSDRGSGSRPG IEGDTPRRGQGREESREPAP ASPAPAGVEIRSATGKEVLQ NLGPKDK in isoform LCRMP-4. 1 PublicationVSP_042546Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78014 mRNA. Translation: BAA11192.1.
    Y07818 mRNA. Translation: CAA69153.1.
    EU007906 mRNA. Translation: ABV80252.1.
    AC011373 Genomic DNA. No translation available.
    CCDSiCCDS43381.1. [Q14195-1]
    CCDS56387.1. [Q14195-2]
    PIRiJC5318.
    RefSeqiNP_001184223.1. NM_001197294.1. [Q14195-2]
    NP_001378.1. NM_001387.2. [Q14195-1]
    UniGeneiHs.519659.

    Genome annotation databases

    EnsembliENST00000343218; ENSP00000343690; ENSG00000113657. [Q14195-2]
    ENST00000398514; ENSP00000381526; ENSG00000113657. [Q14195-1]
    GeneIDi1809.
    KEGGihsa:1809.
    UCSCiuc003lon.1. human. [Q14195-1]
    uc003loo.3. human. [Q14195-2]

    Polymorphism databases

    DMDMi3122050.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78014 mRNA. Translation: BAA11192.1 .
    Y07818 mRNA. Translation: CAA69153.1 .
    EU007906 mRNA. Translation: ABV80252.1 .
    AC011373 Genomic DNA. No translation available.
    CCDSi CCDS43381.1. [Q14195-1 ]
    CCDS56387.1. [Q14195-2 ]
    PIRi JC5318.
    RefSeqi NP_001184223.1. NM_001197294.1. [Q14195-2 ]
    NP_001378.1. NM_001387.2. [Q14195-1 ]
    UniGenei Hs.519659.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BKN X-ray 2.10 A/B 1-570 [» ]
    4CNS X-ray 2.40 A/B/C/D 13-490 [» ]
    4CNU X-ray 2.80 A/B 1-570 [» ]
    ProteinModelPortali Q14195.
    SMRi Q14195. Positions 13-496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108143. 15 interactions.
    IntActi Q14195. 2 interactions.
    STRINGi 9606.ENSP00000381526.

    Protein family/group databases

    MEROPSi M38.976.

    PTM databases

    PhosphoSitei Q14195.

    Polymorphism databases

    DMDMi 3122050.

    2D gel databases

    REPRODUCTION-2DPAGE Q14195.
    UCD-2DPAGE Q14195.

    Proteomic databases

    MaxQBi Q14195.
    PaxDbi Q14195.
    PRIDEi Q14195.

    Protocols and materials databases

    DNASUi 1809.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000343218 ; ENSP00000343690 ; ENSG00000113657 . [Q14195-2 ]
    ENST00000398514 ; ENSP00000381526 ; ENSG00000113657 . [Q14195-1 ]
    GeneIDi 1809.
    KEGGi hsa:1809.
    UCSCi uc003lon.1. human. [Q14195-1 ]
    uc003loo.3. human. [Q14195-2 ]

    Organism-specific databases

    CTDi 1809.
    GeneCardsi GC05M146750.
    HGNCi HGNC:3015. DPYSL3.
    HPAi HPA010948.
    MIMi 601168. gene.
    neXtProti NX_Q14195.
    Orphaneti 803. Amyotrophic lateral sclerosis.
    PharmGKBi PA27473.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0044.
    HOGENOMi HOG000219145.
    HOVERGENi HBG000806.
    OMAi TPRSSQG.
    OrthoDBi EOG7SJD48.
    PhylomeDBi Q14195.
    TreeFami TF314706.

    Enzyme and pathway databases

    Reactomei REACT_19199. CRMPs in Sema3A signaling.

    Miscellaneous databases

    ChiTaRSi DPYSL3. human.
    GeneWikii DPYSL3.
    GenomeRNAii 1809.
    NextBioi 7373.
    PROi Q14195.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14195.
    Bgeei Q14195.
    CleanExi HS_DPYSL3.
    Genevestigatori Q14195.

    Family and domain databases

    Gene3Di 2.30.40.10. 2 hits.
    InterProi IPR006680. Amidohydro_1.
    IPR011778. Hydantoinase/dihydroPyrase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    Pfami PF01979. Amidohydro_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 2 hits.
    TIGRFAMsi TIGR02033. D-hydantoinase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution."
      Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M.
      Gene 180:157-163(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "Identification and characterization of a retinoic acid-regulated human homologue of the unc-33-like phosphoprotein gene (hUlip) from neuroblastoma cells."
      Gaetano C., Matsuo T., Thiele C.J.
      J. Biol. Chem. 272:12195-12201(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Long form collapsin response mediator protein-1 (LCRMP-1) is a novel invasion enhancer: counter-regulation of cancer cell invasion by LCRMP-1 and CRMP-1."
      Pan S.-H., Hong T.-M., Chao Y.-C., Yang S.-C., Yang P.-C.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LCRMP-4).
    4. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 24-56; 82-122; 131-157; 239-254; 259-268; 346-368; 375-397; 401-418; 424-440; 452-480; 488-511 AND 532-550, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 (ISOFORM LCRMP-4), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Distinct priming kinases contribute to differential regulation of collapsin response mediator proteins by glycogen synthase kinase-3 in vivo."
      Cole A.R., Causeret F., Yadirgi G., Hastie C.J., McLauchlan H., McManus E.J., Hernandez F., Eickholt B.J., Nikolic M., Sutherland C.
      J. Biol. Chem. 281:16591-16598(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-522 BY DYRK2, PHOSPHORYLATION AT THR-509; THR-514 AND SER-518 BY GSK3.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259; THR-509; THR-514 AND SER-522, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 (ISOFORM LCRMP-4), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDPYL3_HUMAN
    AccessioniPrimary (citable) accession number: Q14195
    Secondary accession number(s): B3SXQ8, Q93012
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3