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Q14195 (DPYL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropyrimidinase-related protein 3

Short name=DRP-3
Alternative name(s):
Collapsin response mediator protein 4
Short name=CRMP-4
Unc-33-like phosphoprotein 1
Short name=ULIP-1
Gene names
Name:DPYSL3
Synonyms:CRMP4, DRP3, ULIP, ULIP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration By similarity.

Subunit structure

Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5 By similarity. Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin By similarity.

Subcellular location

Cytoplasm By similarity. Cell projectiongrowth cone By similarity. Note: Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone By similarity.

Tissue specificity

Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

Post-translational modification

Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.

Sequence similarities

Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

Caution

Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.

Ontologies

Keywords
   Cellular componentCell projection
Cytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactin crosslink formation

Inferred from sequence or structural similarity. Source: UniProtKB

actin filament bundle assembly

Inferred from sequence or structural similarity. Source: UniProtKB

axon guidance

Traceable author statement. Source: Reactome

cellular response to cytokine stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neuron projection development

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of filopodium assembly

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron projection development

Inferred from sequence or structural similarity. Source: UniProtKB

protein homooligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

pyrimidine nucleobase catabolic process

Inferred from electronic annotation. Source: InterPro

response to axon injury

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcell body

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from sequence or structural similarity. Source: UniProtKB

filamentous actin

Inferred from sequence or structural similarity. Source: UniProtKB

growth cone

Inferred from sequence or structural similarity. Source: UniProtKB

lamellipodium

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionSH3 domain binding

Inferred from sequence or structural similarity. Source: UniProtKB

chondroitin sulfate binding

Inferred from sequence or structural similarity. Source: UniProtKB

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q14195-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform LCRMP-4 (identifier: Q14195-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MSYQGKKNIPRIT → MASGRRGWDS...VLQNLGPKDK
Note: Contains a phosphoserine at position 102.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Dihydropyrimidinase-related protein 3
PRO_0000165917

Amino acid modifications

Modified residue2591Phosphoserine Ref.9
Modified residue4311Phosphotyrosine By similarity
Modified residue5091Phosphothreonine; by GSK3 Ref.7 Ref.9
Modified residue5141Phosphothreonine; by GSK3 Ref.7 Ref.9
Modified residue5181Phosphoserine; by GSK3 Ref.7
Modified residue5221Phosphoserine; by DYRK2 Ref.7 Ref.9

Natural variations

Alternative sequence1 – 1313MSYQG…IPRIT → MASGRRGWDSSHEDDLPVYL ARPGTTDQVPRQKYGGMFCN VEGAFESKTLDFDALSVGQR GAKTPRSGQGSDRGSGSRPG IEGDTPRRGQGREESREPAP ASPAPAGVEIRSATGKEVLQ NLGPKDK in isoform LCRMP-4.
VSP_042546
Natural variant4421A → S.
Corresponds to variant rs2304044 [ dbSNP | Ensembl ].
VAR_020485

Experimental info

Sequence conflict491L → V in CAA69153. Ref.2
Sequence conflict1421T → A in CAA69153. Ref.2

Secondary structure

............................................................................... 570
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9D6AFE86CBB33AD5

FASTA57061,963
        10         20         30         40         50         60 
MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI VPGGVKTIEA 

        70         80         90        100        110        120 
NGKMVIPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG TTMIIDHVVP EPESSLTEAY 

       130        140        150        160        170        180 
EKWREWADGK SCCDYALHVD ITHWNDSVKQ EVQNLIKDKG VNSFMVYMAY KDLYQVSNTE 

       190        200        210        220        230        240 
LYEIFTCLGE LGAIAQVHAE NGDIIAQEQT RMLEMGITGP EGHVLSRPEE LEAEAVFRAI 

       250        260        270        280        290        300 
TIASQTNCPL YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA 

       310        320        330        340        350        360 
FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA IPEGTNGVEE 

       370        380        390        400        410        420 
RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR ISVGSDSDLV IWDPDAVKIV 

       430        440        450        460        470        480 
SAKNHQSAAE YNIFEGMELR GAPLVVICQG KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK 

       490        500        510        520        530        540 
RIKARRKMAD LHAVPRGMYD GPVFDLTTTP KGGTPAGSAR GSPTRPNPPV RNLHQSGFSL 

       550        560        570 
SGTQVDEGVR SASKRIVAPP GGRSNITSLS 

« Hide

Isoform LCRMP-4 [UniParc].

Checksum: 1729A3ED4DA83D30
Show »

FASTA68473,911

References

« Hide 'large scale' references
[1]"A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution."
Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M.
Gene 180:157-163(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Identification and characterization of a retinoic acid-regulated human homologue of the unc-33-like phosphoprotein gene (hUlip) from neuroblastoma cells."
Gaetano C., Matsuo T., Thiele C.J.
J. Biol. Chem. 272:12195-12201(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Long form collapsin response mediator protein-1 (LCRMP-1) is a novel invasion enhancer: counter-regulation of cancer cell invasion by LCRMP-1 and CRMP-1."
Pan S.-H., Hong T.-M., Chao Y.-C., Yang S.-C., Yang P.-C.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LCRMP-4).
[4]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 24-56; 82-122; 131-157; 239-254; 259-268; 346-368; 375-397; 401-418; 424-440; 452-480; 488-511 AND 532-550, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 (ISOFORM LCRMP-4), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Distinct priming kinases contribute to differential regulation of collapsin response mediator proteins by glycogen synthase kinase-3 in vivo."
Cole A.R., Causeret F., Yadirgi G., Hastie C.J., McLauchlan H., McManus E.J., Hernandez F., Eickholt B.J., Nikolic M., Sutherland C.
J. Biol. Chem. 281:16591-16598(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-522 BY DYRK2, PHOSPHORYLATION AT THR-509; THR-514 AND SER-518 BY GSK3.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259; THR-509; THR-514 AND SER-522, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 (ISOFORM LCRMP-4), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78014 mRNA. Translation: BAA11192.1.
Y07818 mRNA. Translation: CAA69153.1.
EU007906 mRNA. Translation: ABV80252.1.
AC011373 Genomic DNA. No translation available.
CCDSCCDS43381.1. [Q14195-1]
CCDS56387.1. [Q14195-2]
PIRJC5318.
RefSeqNP_001184223.1. NM_001197294.1. [Q14195-2]
NP_001378.1. NM_001387.2. [Q14195-1]
UniGeneHs.519659.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BKNX-ray2.10A/B1-570[»]
ProteinModelPortalQ14195.
SMRQ14195. Positions 13-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108143. 15 interactions.
IntActQ14195. 1 interaction.
STRING9606.ENSP00000381526.

Protein family/group databases

MEROPSM38.976.

PTM databases

PhosphoSiteQ14195.

Polymorphism databases

DMDM3122050.

2D gel databases

REPRODUCTION-2DPAGEQ14195.
UCD-2DPAGEQ14195.

Proteomic databases

MaxQBQ14195.
PaxDbQ14195.
PRIDEQ14195.

Protocols and materials databases

DNASU1809.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000343218; ENSP00000343690; ENSG00000113657. [Q14195-2]
ENST00000398514; ENSP00000381526; ENSG00000113657. [Q14195-1]
GeneID1809.
KEGGhsa:1809.
UCSCuc003lon.1. human. [Q14195-1]
uc003loo.3. human. [Q14195-2]

Organism-specific databases

CTD1809.
GeneCardsGC05M146750.
HGNCHGNC:3015. DPYSL3.
HPAHPA010948.
MIM601168. gene.
neXtProtNX_Q14195.
Orphanet803. Amyotrophic lateral sclerosis.
PharmGKBPA27473.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0044.
HOGENOMHOG000219145.
HOVERGENHBG000806.
OMATPRSSQG.
OrthoDBEOG7SJD48.
PhylomeDBQ14195.
TreeFamTF314706.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ14195.
BgeeQ14195.
CleanExHS_DPYSL3.
GenevestigatorQ14195.

Family and domain databases

Gene3D2.30.40.10. 2 hits.
InterProIPR006680. Amidohydro_1.
IPR011778. Hydantoinase/dihydroPyrase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. SSF51338. 2 hits.
TIGRFAMsTIGR02033. D-hydantoinase. 1 hit.
ProtoNetSearch...

Other

ChiTaRSDPYSL3. human.
GeneWikiDPYSL3.
GenomeRNAi1809.
NextBio7373.
PROQ14195.
SOURCESearch...

Entry information

Entry nameDPYL3_HUMAN
AccessionPrimary (citable) accession number: Q14195
Secondary accession number(s): B3SXQ8, Q93012
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM