Reviewed, UniProtKB/Swiss-Prot Q14194 (DPYL1_HUMAN)

Names and origin

Protein names

Recommended name:
    Dihydropyrimidinase-related protein 1
      Short name=DRP-1
Alternative name(s):
    Collapsin response mediator protein 1
      Short name=CRMP-1

Gene names

Name:	CRMP1
Synonyms:	DPYSL1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Ref.8
Subunit structure	Homotetramer, and heterotetramer with DPYSL2, DPYSL3, DPYSL4 or DPYSL5. Interacts with PLXNA1 By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle. Note: Associated with centrosomes and the mitotic spindle during metaphase. Ref.8
Tissue specificity	Brain.
Sequence similarities	Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
Caution	Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nervous system development Ref.1 Traceable author statement. Source: ProtInc nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydropyrimidinase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AXIN1	O15169	1	EBI-473101,EBI-710484
HTT	P42858	1	EBI-473101,EBI-466029
VIM	P08670	1	EBI-473101,EBI-353844

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Dihydropyrimidinase-related protein 1		PRO_0000165909
Amino acid modifications
	Modified residue	431	1	Phosphotyrosine By similarity
	Modified residue	504	1	Phosphotyrosine By similarity
	Modified residue	509	1	Phosphothreonine By similarity
Natural variations
	Natural variant	461	1	V → I: dbSNP rs34611001.		VAR_037745
Experimental info
	Sequence conflict	351 – 370	20	Missing in AAK55500. Ref.4
	Sequence conflict	504	1	Y → H in AAA93201. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q14194-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A5385FCC79328A30	FASTA	572	62,184
	10 20 30 40 50 60 MSYQGKKSIP HITSDRLLIK GGRIINDDQS LYADVYLEDG LIKQIGENLI VPGGVKTIEA 70 80 90 100 110 120 NGRMVIPGGI DVNTYLQKPS QGMTAADDFF QGTRAALVGG TTMIIDHVVP EPGSSLLTSF 130 140 150 160 170 180 EKWHEAADTK SCCDYSLHVD ITSWYDGVRE ELEVLVQDKG VNSFQVYMAY KDVYQMSDSQ 190 200 210 220 230 240 LYEAFTFLKG LGAVILVHAE NGDLIAQEQK RILEMGITGP EGHALSRPEE LEAEAVFRAI 250 260 270 280 290 300 TIAGRINCPV YITKVMSKSA ADIIALARKK GPLVFGEPIA ASLGTDGTHY WSKNWAKAAA 310 320 330 340 350 360 FVTSPPLSPD PTTPDYLTSL LACGDLQVTG SGHCPYSTAQ KAVGKDNFTL IPEGVNGIEE 370 380 390 400 410 420 RMTVVWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR IAVGSDADVV IWDPDKLKTI 430 440 450 460 470 480 TAKSHKSAVE YNIFEGMECH GSPLVVISQG KIVFEDGNIN VNKGMGRFIP RKAFPEHLYQ 490 500 510 520 530 540 RVKIRNKVFG LQGVSRGMYD GPVYEVPATP KYATPAPSAK SSPSKHQPPP IRNLHQSNFS 550 560 570 LSGAQIDDNN PRRTGHRIVA PPGGRSNITS LG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution." Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M. Gene 180:157-163(1996) [PubMed: 8973361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[4]	"Genomic organization and localization of the human CRMP-1 gene." Torres R., Polymeropoulos M.H. DNA Res. 5:393-395(1998) [PubMed: 10048489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-572.
[5]	"Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33." Goshima Y., Nakamura F., Strittmatter P., Strittmatter S.M. Nature 376:509-514(1995) [PubMed: 7637782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-572. Tissue: Brain.
[6]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 44-56; 190-210; 246-254; 259-268; 346-361; 391-397; 452-463; 472-481; 488-511 AND 533-552, MASS SPECTROMETRY. Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[7]	"Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase." Wang L.H., Strittmatter S.M. J. Neurochem. 69:2261-2269(1997) [PubMed: 9375656] [Abstract] Cited for: SUBUNIT, INTERACTION WITH DPYSL2 AND DPYSL4.
[8]	"Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells." Shih J.-Y., Yang S.-C., Hong T.-M., Yuan A., Chen J.J.W., Yu C.-J., Chang Y.-L., Lee Y.-C., Peck K., Wu C.-W., Yang P.-C. J. Natl. Cancer Inst. 93:1392-1400(2001) [PubMed: 11562390] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78012 mRNA. Translation: BAA11190.1. BT006806 mRNA. Translation: AAP35452.1. BC000252 mRNA. Translation: AAH00252.1. BC007613 mRNA. Translation: AAH07613.1. AF096152 , AF096140, AF096141, AF096142, AF096143, AF096144, AF096145, AF096146, AF096147, AF096148, AF096149, AF096150, AF096151 Genomic DNA. Translation: AAK55500.1. U17278 mRNA. Translation: AAA93201.1.
IPI	IPI00414123.
PIR	JC5316.
RefSeq	NP_001304.1.
UniGene	Hs.135270
3D structure databases
HSSP	HSSP built from PDB template 1K1D based on UniProtKB Q45515.
SMR	Q14194. Positions 15-490.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14194. 54 interactions.
STRING	Q14194.
Protein family/group databases
MEROPS	M38.974.
PTM databases
PhosphoSite	Q14194.
Proteomic databases
PRIDE	Q14194.
Genome annotation databases
Ensembl	ENST00000324989; ENSP00000321606; ENSG00000072832; Homo sapiens. [Genome view] ENST00000397890; ENSP00000380987; ENSG00000072832; Homo sapiens. [Genome view]
GeneID	1400.
UCSC	uc003gip.1. human.
Organism-specific databases
CTD	1400.
GeneCards	GC04M005940.
H-InvDB	HIX0004057.
HGNC	HGNC:2365. CRMP1.
MIM	602462. gene.
PharmGKB	PA26885.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q14194.
HOVERGEN	Q14194.
Enzyme and pathway databases
Reactome	REACT_18266. Axon guidance.
Gene expression databases
ArrayExpress	Q14194.
Bgee	Q14194.
CleanEx	HS_CRMP1.
Genevestigator	Q14194.
GermOnline	ENSG00000072832. Homo sapiens.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. IPR011778. D-hydantoinase. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02033. D-hydantoinase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	5731.
SOURCE	Search...

Entry information

Entry name

DPYL1_HUMAN

Accession

Primary (citable) accession number: Q14194
Secondary accession number(s): Q13024, Q96TC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.