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Q14194 (DPYL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropyrimidinase-related protein 1

Short name=DRP-1
Alternative name(s):
Collapsin response mediator protein 1
Short name=CRMP-1
Unc-33-like phosphoprotein 3
Short name=ULIP-3
Gene names
Name:CRMP1
Synonyms:DPYSL1, ULIP3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May participate in cytokinesis. Ref.11 Ref.12

Subunit structure

Homotetramer, and heterotetramer with DPYSL2, DPYSL3, DPYSL4 or DPYSL5. Interacts with PLXNA1 By similarity. Ref.10

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle. Note: Associated with centrosomes and the mitotic spindle during metaphase. Ref.11

Tissue specificity

Brain.

Sequence similarities

Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

Caution

Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q14194-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform LCRMP-1 (identifier: Q14194-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MSYQGKKSIPHIT → MADRRRAWNT...DLPLGRDNGQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 572572Dihydropyrimidinase-related protein 1
PRO_0000165909

Amino acid modifications

Modified residue1011Phosphothreonine; by AURKA Ref.12
Modified residue1021Phosphothreonine; by AURKA Ref.12
Modified residue3161Nitrated tyrosine By similarity
Modified residue5041Phosphotyrosine By similarity
Modified residue5091Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 1313MSYQG…IPHIT → MADRRRAWNTEDDLPVYLAR PGSAAQTPRQKYGGMFAAVE GAYENKTIDFDAYSVGRRGS ARTPRSAGRPDAVGLPGPGG SEDTASDVSEPSGSAVSSPG ERDERPPTLRIRRPAPRDLP LGRDNGQ in isoform LCRMP-1.
VSP_042545
Natural variant4611V → I.
Corresponds to variant rs34611001 [ dbSNP | Ensembl ].
VAR_037745

Experimental info

Mutagenesis101 – 1022TT → AA: 2.5-fold increase in cells with a defect of cytokinesis. Ref.12
Sequence conflict351 – 37020Missing in AAK55500. Ref.7
Sequence conflict5041Y → H in AAA93201. Ref.8

Secondary structure

............................................................................. 572
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A5385FCC79328A30

FASTA57262,184
        10         20         30         40         50         60 
MSYQGKKSIP HITSDRLLIK GGRIINDDQS LYADVYLEDG LIKQIGENLI VPGGVKTIEA 

        70         80         90        100        110        120 
NGRMVIPGGI DVNTYLQKPS QGMTAADDFF QGTRAALVGG TTMIIDHVVP EPGSSLLTSF 

       130        140        150        160        170        180 
EKWHEAADTK SCCDYSLHVD ITSWYDGVRE ELEVLVQDKG VNSFQVYMAY KDVYQMSDSQ 

       190        200        210        220        230        240 
LYEAFTFLKG LGAVILVHAE NGDLIAQEQK RILEMGITGP EGHALSRPEE LEAEAVFRAI 

       250        260        270        280        290        300 
TIAGRINCPV YITKVMSKSA ADIIALARKK GPLVFGEPIA ASLGTDGTHY WSKNWAKAAA 

       310        320        330        340        350        360 
FVTSPPLSPD PTTPDYLTSL LACGDLQVTG SGHCPYSTAQ KAVGKDNFTL IPEGVNGIEE 

       370        380        390        400        410        420 
RMTVVWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR IAVGSDADVV IWDPDKLKTI 

       430        440        450        460        470        480 
TAKSHKSAVE YNIFEGMECH GSPLVVISQG KIVFEDGNIN VNKGMGRFIP RKAFPEHLYQ 

       490        500        510        520        530        540 
RVKIRNKVFG LQGVSRGMYD GPVYEVPATP KYATPAPSAK SSPSKHQPPP IRNLHQSNFS 

       550        560        570 
LSGAQIDDNN PRRTGHRIVA PPGGRSNITS LG 

« Hide

Isoform LCRMP-1 [UniParc].

Checksum: 2C897F656E5F34FA
Show »

FASTA68674,262

References

« Hide 'large scale' references
[1]"A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution."
Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M.
Gene 180:157-163(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Long form collapsin response mediator protein-1 (LCRMP-1) expression is associated with clinical outcome and lymph node metastasis in non-small cell lung cancer patients."
Pan S.-H., Chao Y.-C., Chen H.Y., Hung P.F., Lin P.Y., Lin C.W., Chang Y.L., Wu C.T., Lee Y.C., Yang S.-C., Hong T.-M., Yang P.-C.
Lung Cancer 67:93-100(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LCRMP-1), ALTERNATIVE SPLICING.
Tissue: Lung adenocarcinoma.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
[7]"Genomic organization and localization of the human CRMP-1 gene."
Torres R., Polymeropoulos M.H.
DNA Res. 5:393-395(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-572 (ISOFORM 1).
[8]"Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33."
Goshima Y., Nakamura F., Strittmatter P., Strittmatter S.M.
Nature 376:509-514(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-572 (ISOFORM 1).
Tissue: Brain.
[9]Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 44-56; 190-210; 246-254; 259-268; 346-361; 391-397; 452-463; 472-481; 488-511 AND 533-552, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[10]"Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase."
Wang L.H., Strittmatter S.M.
J. Neurochem. 69:2261-2269(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH DPYSL2 AND DPYSL4.
[11]"Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells."
Shih J.-Y., Yang S.-C., Hong T.-M., Yuan A., Chen J.J.W., Yu C.-J., Chang Y.-L., Lee Y.-C., Peck K., Wu C.-W., Yang P.-C.
J. Natl. Cancer Inst. 93:1392-1400(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]"From midbody protein-protein interaction network construction to novel regulators in cytokinesis."
Chen T.C., Lee S.A., Hong T.M., Shih J.Y., Lai J.M., Chiou H.Y., Yang S.C., Chan C.H., Kao C.Y., Yang P.C., Huang C.Y.
J. Proteome Res. 8:4943-4953(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT THR-101 AND THR-102, MUTAGENESIS OF 101-THR-THR-102.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78012 mRNA. Translation: BAA11190.1.
DQ206871 mRNA. Translation: ABB22046.1.
BT006806 mRNA. Translation: AAP35452.1.
AC105915 Genomic DNA. Translation: AAY40959.1.
CH471131 Genomic DNA. Translation: EAW82405.1.
CH471131 Genomic DNA. Translation: EAW82407.1.
BC000252 mRNA. Translation: AAH00252.1.
BC007613 mRNA. Translation: AAH07613.1.
AH010780 Genomic DNA. Translation: AAK55500.1.
U17278 mRNA. Translation: AAA93201.1.
CCDSCCDS33950.1. [Q14194-2]
CCDS43207.1. [Q14194-1]
PIRJC5316.
RefSeqNP_001014809.1. NM_001014809.2. [Q14194-2]
NP_001275590.1. NM_001288661.1.
NP_001275591.1. NM_001288662.1.
NP_001304.1. NM_001313.4. [Q14194-1]
UniGeneHs.135270.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4B3ZX-ray3.05A/B/C/D1-572[»]
ProteinModelPortalQ14194.
SMRQ14194. Positions 15-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107790. 83 interactions.
IntActQ14194. 71 interactions.
MINTMINT-1423114.
STRING9606.ENSP00000321606.

Protein family/group databases

MEROPSM38.974.

PTM databases

PhosphoSiteQ14194.

Polymorphism databases

DMDM3122031.

Proteomic databases

MaxQBQ14194.
PaxDbQ14194.
PRIDEQ14194.

Protocols and materials databases

DNASU1400.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000324989; ENSP00000321606; ENSG00000072832. [Q14194-2]
ENST00000397890; ENSP00000380987; ENSG00000072832. [Q14194-1]
GeneID1400.
KEGGhsa:1400.
UCSCuc003giq.3. human. [Q14194-1]
uc003gis.3. human. [Q14194-2]

Organism-specific databases

CTD1400.
GeneCardsGC04M005815.
HGNCHGNC:2365. CRMP1.
HPAHPA035640.
MIM602462. gene.
neXtProtNX_Q14194.
PharmGKBPA26885.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0044.
HOGENOMHOG000219145.
HOVERGENHBG000806.
OMAEDIHTGT.
OrthoDBEOG7SJD48.
PhylomeDBQ14194.
TreeFamTF314706.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ14194.
BgeeQ14194.
CleanExHS_CRMP1.
GenevestigatorQ14194.

Family and domain databases

Gene3D2.30.40.10. 2 hits.
InterProIPR006680. Amidohydro_1.
IPR011778. Hydantoinase/dihydroPyrase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. SSF51338. 2 hits.
TIGRFAMsTIGR02033. D-hydantoinase. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCRMP1. human.
GeneWikiCRMP1.
GenomeRNAi1400.
NextBio5731.
PROQ14194.
SOURCESearch...

Entry information

Entry nameDPYL1_HUMAN
AccessionPrimary (citable) accession number: Q14194
Secondary accession number(s): A0EJG6 expand/collapse secondary AC list , Q13024, Q4W5F1, Q96TC8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM