Q14168 (MPP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAGUK p55 subfamily member 2

Alternative name(s):
Discs large homolog 2
Protein MPP2

Gene names

Name:	MPP2
Synonyms:	DLG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 2 L27 domains. Contains 1 PDZ (DHR) domain. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Traceable author statement Ref.1PubMed 8824795. Source: ProtInc
Cellular_component	cell surface Inferred from direct assay PubMed 12493773. Source: UniProtKB integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	guanylate kinase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14168-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14168-2)

The sequence of this isoform differs from the canonical sequence as follows:
     51-74: Missing.

Isoform 3 (identifier: Q14168-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MPVAATNSET → MAGSPGSGVSLEGISLESSEEAELQRE
     51-74: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 576

576

MAGUK p55 subfamily member 2

PRO_0000094573

Regions

Domain

8 – 60

53

L27 1

Domain

84 – 142

59

L27 2

Domain

185 – 240

56

PDZ

Domain

249 – 317

69

SH3

Domain

374 – 561

188

Guanylate kinase-like

Amino acid modifications

Modified residue

42

1

Phosphoserine By similarity

Modified residue

141

1

Phosphothreonine Ref.7

Natural variations

Alternative sequence

1 – 10

10

MPVAATNSET → MAGSPGSGVSLEGISLESSE EAELQRE in isoform 3.

VSP_022951

Alternative sequence

51 – 74

24

Missing in isoform 2 and isoform 3.

VSP_003156

Experimental info

Sequence conflict

80

1

E → G in CAI56746. Ref.4

Sequence conflict

104 – 105

2

HV → QL in CAA58067. Ref.1

Sequence conflict

104 – 105

2

HV → QL in BAD97280. Ref.2

Sequence conflict

104 – 105

2

HV → QL in CAB66489. Ref.3

Sequence conflict

104 – 105

2

HV → QL in CAI56746. Ref.4

Sequence conflict

104 – 105

2

HV → QL in AAH30287. Ref.6

Sequence conflict

242

1

S → N in CAA58067. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

576

Helix Strand Turn

Details...

Beta strand

177 – 191

15

Beta strand

193 – 195

3

Helix

196 – 200

5

Beta strand

208 – 212

5

Helix

221 – 229

9

Beta strand

233 – 235

3

Beta strand

237 – 240

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 2, 2010. Version 3. Checksum: A08E858EA646A305	FASTA	576	64,581
	10 20 30 40 50 60 MPVAATNSET AMQQVLDNLG SLPSATGAAE LDLIFLRGIM ESPIVRSLAK VIMVLWFMQQ 70 80 90 100 110 120 NVFVPMKYML KYFGAHERLE ETKLEAVRDN NLELVQEILR DLAHVAEQSS TAAELAHILQ 130 140 150 160 170 180 EPHFQSLLET HDSVASKTYE TPPPSPGLDP TFSNQPVPPD AVRMVGIRKT AGEHLGVTFR 190 200 210 220 230 240 VEGGELVIAR ILHGGMVAQQ GLLHVGDIIK EVNGQPVGSD PRALQELLRN ASGSVILKIL 250 260 270 280 290 300 PSYQEPHLPR QVFVKCHFDY DPARDSLIPC KEAGLRFNAG DLLQIVNQDD ANWWQACHVE 310 320 330 340 350 360 GGSAGLIPSQ LLEEKRKAFV KRDLELTPNS GTLCGSLSGK KKKRMMYLTT KNAEFDRHEL 370 380 390 400 410 420 LIYEEVARMP PFRRKTLVLI GAQGVGRRSL KNKLIMWDPD RYGTTVPYTS RRPKDSEREG 430 440 450 460 470 480 QGYSFVSRGE MEADVRAGRY LEHGEYEGNL YGTRIDSIRG VVAAGKVCVL DVNPQAVKVL 490 500 510 520 530 540 RTAEFVPYVV FIEAPDFETL RAMNRAALES GISTKQLTEA DLRRTVEESS RIQRGYGHYF 550 560 570 DLCLVNSNLE RTFRELQTAM EKLRTEPQWV PVSWVY
	Isoform 2 [UniParc]. Checksum: 81BBB336B3C9DA89	FASTA	552	61,585
	10 20 30 40 50 60 MPVAATNSET AMQQVLDNLG SLPSATGAAE LDLIFLRGIM ESPIVRSLAK AHERLEETKL 70 80 90 100 110 120 EAVRDNNLEL VQEILRDLAH VAEQSSTAAE LAHILQEPHF QSLLETHDSV ASKTYETPPP 130 140 150 160 170 180 SPGLDPTFSN QPVPPDAVRM VGIRKTAGEH LGVTFRVEGG ELVIARILHG GMVAQQGLLH 190 200 210 220 230 240 VGDIIKEVNG QPVGSDPRAL QELLRNASGS VILKILPSYQ EPHLPRQVFV KCHFDYDPAR 250 260 270 280 290 300 DSLIPCKEAG LRFNAGDLLQ IVNQDDANWW QACHVEGGSA GLIPSQLLEE KRKAFVKRDL 310 320 330 340 350 360 ELTPNSGTLC GSLSGKKKKR MMYLTTKNAE FDRHELLIYE EVARMPPFRR KTLVLIGAQG 370 380 390 400 410 420 VGRRSLKNKL IMWDPDRYGT TVPYTSRRPK DSEREGQGYS FVSRGEMEAD VRAGRYLEHG 430 440 450 460 470 480 EYEGNLYGTR IDSIRGVVAA GKVCVLDVNP QAVKVLRTAE FVPYVVFIEA PDFETLRAMN 490 500 510 520 530 540 RAALESGIST KQLTEADLRR TVEESSRIQR GYGHYFDLCL VNSNLERTFR ELQTAMEKLR 550 TEPQWVPVSW VY
	Isoform 3 [UniParc]. Checksum: 40FE59BE6B6D998C	FASTA	569	63,315
	10 20 30 40 50 60 MAGSPGSGVS LEGISLESSE EAELQREAMQ QVLDNLGSLP SATGAAELDL IFLRGIMESP 70 80 90 100 110 120 IVRSLAKAHE RLEETKLEAV RDNNLELVQE ILRDLAHVAE QSSTAAELAH ILQEPHFQSL 130 140 150 160 170 180 LETHDSVASK TYETPPPSPG LDPTFSNQPV PPDAVRMVGI RKTAGEHLGV TFRVEGGELV 190 200 210 220 230 240 IARILHGGMV AQQGLLHVGD IIKEVNGQPV GSDPRALQEL LRNASGSVIL KILPSYQEPH 250 260 270 280 290 300 LPRQVFVKCH FDYDPARDSL IPCKEAGLRF NAGDLLQIVN QDDANWWQAC HVEGGSAGLI 310 320 330 340 350 360 PSQLLEEKRK AFVKRDLELT PNSGTLCGSL SGKKKKRMMY LTTKNAEFDR HELLIYEEVA 370 380 390 400 410 420 RMPPFRRKTL VLIGAQGVGR RSLKNKLIMW DPDRYGTTVP YTSRRPKDSE REGQGYSFVS 430 440 450 460 470 480 RGEMEADVRA GRYLEHGEYE GNLYGTRIDS IRGVVAAGKV CVLDVNPQAV KVLRTAEFVP 490 500 510 520 530 540 YVVFIEAPDF ETLRAMNRAA LESGISTKQL TEADLRRTVE ESSRIQRGYG HYFDLCLVNS 550 560 NLERTFRELQ TAMEKLRTEP QWVPVSWVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene (DLG2) located at 17q12-q21 encodes a new homologue of the Drosophila tumor suppressor dlg-A." Mazoyer S., Gayther S.A., Nagai M.A., Smith S.A., Dunning A., van Rensburg E.J., Albertsen H., White R., Ponder B.A.J. Genomics 28:25-31(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Amygdala.
[3]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Salivary gland.
[5]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-141, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Solution structure of the PDZ domain from human maguk p55 subfamily member 2." RIKEN structural genomics initiative (RSGI) Submitted (JAN-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 163-240.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X82895 mRNA. Translation: CAA58067.1.
AK223560 mRNA. Translation: BAD97280.1.
AL136554 mRNA. Translation: CAB66489.1.
CR936598 mRNA. Translation: CAI56746.1.
AC007993 Genomic DNA. No translation available.
BC030287 mRNA. Translation: AAH30287.1.

IPI

IPI00218271.
IPI00956110.
IPI01011986.

PIR

A57653.

RefSeq

NP_005365.3. NM_005374.3.

UniGene

Hs.514208.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E7K	NMR	-	A	163-240	[»]

ProteinModelPortal

Q14168.

SMR

Q14168. Positions 163-240, 252-571.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14168. 2 interactions.

STRING

9606.ENSP00000269095.

PTM databases

PhosphoSite

Q14168.

Polymorphism databases

DMDM

290457681.

Proteomic databases

PaxDb

Q14168.

PRIDE

Q14168.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000269095; ENSP00000269095; ENSG00000108852.
ENST00000377184; ENSP00000366389; ENSG00000108852.
ENST00000461854; ENSP00000428286; ENSG00000108852.

GeneID

4355.

KEGG

hsa:4355.

UCSC

uc002ien.1. human.
uc002ieo.1. human.
uc010win.1. human.

Organism-specific databases

CTD

4355.

GeneCards

GC17M041963.

H-InvDB

HIX0013870.

HGNC

HGNC:7220. MPP2.

HPA

HPA026486.

MIM

600723. gene.

neXtProt

NX_Q14168.

PharmGKB

PA30925.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0194.

HOGENOM

HOG000233034.

HOVERGEN

HBG001858.

InParanoid

Q14168.

PhylomeDB

Q14168.

Gene expression databases

ArrayExpress

Q14168.

Bgee

Q14168.

CleanEx

HS_DLG2.
HS_MPP2.

Genevestigator

Q14168.

GermOnline

ENSG00000108852. Homo sapiens.

Family and domain databases

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylate_kin/L-typ_Ca_channel.
IPR020590. Guanylate_kinase_CS.
IPR004172. L27.
IPR014775. L27_C.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00625. Guanylate_kin. 1 hit.
PF02828. L27. 2 hits.
PF00595. PDZ. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]

SMART

SM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.
SSF50044. SH3. 1 hit.

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q14168.

GenomeRNAi

4355.

NextBio

17134.

SOURCE

Search...

Entry information

Entry name

MPP2_HUMAN

Accession

Primary (citable) accession number: Q14168
Secondary accession number(s): Q53ES9, Q5CZB9, Q9BQJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.