Reviewed,
UniProtKB/Swiss-Prot Q14161 (GIT2_HUMAN)
Last modified
November 3, 2009.
Version 105.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: ARF GTPase-activating protein GIT2 Short name=ARF GAP GIT2 Alternative name(s): G protein-coupled receptor kinase-interactor 2 GRK-interacting protein 2 Cool-interacting tyrosine-phosphorylated protein 2 Short name=CAT-2 Short name=CAT2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 759 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | GTPase-activating protein for the ADP ribosylation factor family. |
| Subunit structure | Interacts with TGFB1I1 By similarity. Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. |
| Sequence similarities | Contains 3 ANK repeats. Contains 1 Arf-GAP domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | ANK repeat Repeat Zinc-finger |
| Ligand | Metal-binding Zinc |
| Molecular function | GTPase activation |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro regulation of G-protein coupled receptor protein signaling pathway Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | nucleoplasm Inferred from direct assay. Source: UniProtKB |
| Molecular function | ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 11 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: Q14161-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q14161-2) Also known as: GIT2-short; The sequence of this isoform differs from the canonical sequence as follows: 466-471: QTLQSE → LGKDAN 472-759: Missing. | ||||||
| Isoform 3 (identifier: Q14161-3) Also known as: C-; The sequence of this isoform differs from the canonical sequence as follows: 450-464: Missing. | ||||||
| Isoform 4 (identifier: Q14161-4) Also known as: BC-; The sequence of this isoform differs from the canonical sequence as follows: 415-449: Missing. 450-464: Missing. | ||||||
| Isoform 5 (identifier: Q14161-5) Also known as: E-; The sequence of this isoform differs from the canonical sequence as follows: 548-577: Missing. | ||||||
| Isoform 6 (identifier: Q14161-6) Also known as: CD-; The sequence of this isoform differs from the canonical sequence as follows: 450-464: Missing. 465-547: Missing. | ||||||
| Isoform 7 (identifier: Q14161-7) Also known as: DE-; The sequence of this isoform differs from the canonical sequence as follows: 465-547: Missing. 548-577: Missing. | ||||||
| Isoform 8 (identifier: Q14161-8) Also known as: BE-; The sequence of this isoform differs from the canonical sequence as follows: 415-449: Missing. 548-577: Missing. | ||||||
| Isoform 9 (identifier: Q14161-9) Also known as: AE-; The sequence of this isoform differs from the canonical sequence as follows: 334-414: Missing. 548-577: Missing. | ||||||
| Isoform 10 (identifier: Q14161-10) The sequence of this isoform differs from the canonical sequence as follows: 255-255: S → RRL 414-463: Missing. 548-577: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 11 (identifier: Q14161-11) The sequence of this isoform differs from the canonical sequence as follows: 450-464: Missing. 465-547: Missing. 548-577: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 759 | 759 | ARF GTPase-activating protein GIT2 | PRO_0000074203 | |||||
Regions | |||||||||
| Domain | 1 – 124 | 124 | Arf-GAP | ||||||
| Repeat | 132 – 161 | 30 | ANK 1 | ||||||
| Repeat | 166 – 195 | 30 | ANK 2 | ||||||
| Repeat | 199 – 228 | 30 | ANK 3 | ||||||
| Zinc finger | 11 – 34 | 24 | C4-type | ||||||
Amino acid modifications | |||||||||
| Modified residue | 394 | 1 | Phosphoserine Ref.9 Ref.11 | ||||||
| Modified residue | 397 | 1 | Phosphoserine Ref.9 Ref.11 | ||||||
| Modified residue | 401 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 415 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 418 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 421 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 484 | 1 | Phosphotyrosine Ref.8 | ||||||
| Modified residue | 488 | 1 | Phosphothreonine Ref.7 | ||||||
| Modified residue | 492 | 1 | Phosphotyrosine Ref.8 | ||||||
| Modified residue | 496 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 559 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 586 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 614 | 1 | Phosphoserine Ref.10 | ||||||
Natural variations | |||||||||
| Alternative sequence | 255 | 1 | S → RRL in isoform 10. | VSP_026456 | |||||
| Alternative sequence | 334 – 414 | 81 | Missing in isoform 9. | VSP_000305 | |||||
| Alternative sequence | 414 – 463 | 50 | Missing in isoform 10. | VSP_008654 | |||||
| Alternative sequence | 415 – 449 | 35 | Missing in isoform 4 and isoform 8. | VSP_000306 | |||||
| Alternative sequence | 450 – 464 | 15 | Missing in isoform 3, isoform 4, isoform 6 and isoform 11. | VSP_000307 | |||||
| Alternative sequence | 465 – 547 | 83 | Missing in isoform 6, isoform 7 and isoform 11. | VSP_000308 | |||||
| Alternative sequence | 466 – 471 | 6 | QTLQSE → LGKDAN in isoform 2. | VSP_000303 | |||||
| Alternative sequence | 472 – 759 | 288 | Missing in isoform 2. | VSP_000304 | |||||
| Alternative sequence | 548 – 577 | 30 | Missing in isoform 5, isoform 7, isoform 8, isoform 9, isoform 10 and isoform 11. | VSP_000309 | |||||
| Natural variant | 338 | 1 | N → S: dbSNP rs9804905. | VAR_048324 | |||||
| Natural variant | 387 | 1 | N → S: dbSNP rs925368. | VAR_024368 | |||||
| Natural variant | 552 | 1 | A → V: dbSNP rs11068997. | VAR_048325 | |||||
Experimental info | |||||||||
| Sequence conflict | 285 | 1 | V → M in AAP35976. Ref.5 | ||||||
| Sequence conflict | 285 | 1 | V → M in AAH01379. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing." Premont R.T., Claing A., Vitale N., Perry S.J., Lefkowitz R.J. J. Biol. Chem. 275:22373-22380(2000) [PubMed: 10896954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5; 6; 7; 8 AND 9), CHARACTERIZATION. |
| [2] | "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed: 8590280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 10). Tissue: Bone marrow. |
| [3] | "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION. |
| [4] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 29-759 (ISOFORM 11). Tissue: B-cell and Skin. |
| [6] | "An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization." Mazaki Y., Hashimoto S., Okawa K., Tsubouchi A., Nakamura K., Yagi R., Yano H., Kondo A., Iwamatsu A., Mizoguchi A., Sabe H. Mol. Biol. Cell 12:645-662(2001) [PubMed: 11251077] [Abstract] Cited for: INTERACTION WITH PAXILLIN. |
| [7] | "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-488 AND SER-496, MASS SPECTROMETRY. Tissue: T-cell. |
| [8] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-484 AND TYR-492, MASS SPECTROMETRY. |
| [9] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394; SER-397; SER-415; SER-418 AND SER-421, MASS SPECTROMETRY. Tissue: Epithelium. |
| [10] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-559 AND SER-614, MASS SPECTROMETRY. |
| [11] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394 AND SER-397, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF124491 mRNA. Translation: AAD28047.1. D63482 mRNA. Translation: BAA09769.2. Different initiation. BT007312 mRNA. Translation: AAP35976.1. BC001379 mRNA. Translation: AAH01379.1. BC014223 mRNA. Translation: AAH14223.2. | |
| IPI | IPI00186690. IPI00216991. IPI00216993. IPI00216994. IPI00216996. IPI00216997. IPI00216998. IPI00238218. IPI00376496. IPI00413167. IPI00413822. |
| RefSeq | NP_001128685.1. NP_001128686.1. NP_055591.2. NP_476510.1. NP_476511.1. |
| UniGene | Hs.434996 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OY3 based on UniProtKB Q60778. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q14161. 1 interaction. |
| STRING | Q14161. |
PTM databases | |
| PhosphoSite | Q14161. |
Proteomic databases | |
| PRIDE | Q14161. |
Genome annotation databases | |
| Ensembl | ENST00000320063; ENSP00000323833; ENSG00000139436; Homo sapiens. [Genome view] ENST00000338373; ENSP00000340342; ENSG00000139436; Homo sapiens. [Genome view] ENST00000343646; ENSP00000340938; ENSG00000139436; Homo sapiens. [Genome view] ENST00000354574; ENSP00000346585; ENSG00000139436; Homo sapiens. [Genome view] ENST00000355312; ENSP00000347464; ENSG00000139436; Homo sapiens. [Genome view] ENST00000356259; ENSP00000348595; ENSG00000139436; Homo sapiens. [Genome view] ENST00000360185; ENSP00000353312; ENSG00000139436; Homo sapiens. [Genome view] ENST00000361006; ENSP00000354282; ENSG00000139436; Homo sapiens. [Genome view] ENST00000457474; ENSP00000391813; ENSG00000139436; Homo sapiens. [Genome view] |
| GeneID | 9815. |
| KEGG | hsa:9815. |
| UCSC | uc001tps.1. human. uc001tpt.1. human. uc001tpu.1. human. |
Organism-specific databases | |
| CTD | 9815. |
| GeneCards | GC12M108830. |
| H-InvDB | HIX0010983. HIX0010985. |
| HGNC | HGNC:4273. GIT2. |
| HPA | CAB004681. |
| MIM | 608564. gene. |
| PharmGKB | PA28684. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q14161. |
| HOVERGEN | Q14161. |
| OMA | PMSMYET. |
Gene expression databases | |
| ArrayExpress | Q14161. |
| Bgee | Q14161. |
| CleanEx | HS_GIT2. |
| Genevestigator | Q14161. |
| GermOnline | ENSG00000139436. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR002110. ANK. IPR001164. ArfGAP. IPR013724. GIT_SHD. [Graphical view] |
| Gene3D | G3DSA:1.25.40.20. ANK. 1 hit. |
| Pfam | PF00023. Ank. 3 hits. PF01412. ArfGap. 1 hit. PF08518. GIT_SHD. 2 hits. [Graphical view] |
| PRINTS | PR00405. REVINTRACTNG. |
| SMART | SM00248. ANK. 3 hits. SM00105. ArfGap. 1 hit. SM00555. GIT. 2 hits. [Graphical view] |
| PROSITE | PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS50115. ARFGAP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 36954. |
| SOURCE | Search... |
Entry information
| Entry name | GIT2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q14161 Secondary accession number(s): Q86U59 Q9Y5V2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


