Q14161 (GIT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ARF GTPase-activating protein GIT2
Short name=ARF GAP GIT2

Alternative name(s):
Cool-interacting tyrosine-phosphorylated protein 2
Short name=CAT-2
Short name=CAT2
G protein-coupled receptor kinase-interactor 2
GRK-interacting protein 2

Gene names

Name:	GIT2
Synonyms:	KIAA0148

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein for the ADP ribosylation factor family.
Subunit structure	Interacts with TGFB1I1 By similarity. Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Identified in a complex with ARHGEF6 and BIN2. Ref.6 Ref.15
Sequence similarities	Contains 3 ANK repeats. Contains 1 Arf-GAP domain.
Sequence caution	The sequence BAA09769.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro regulation of G-protein coupled receptor protein signaling pathway Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nucleoplasm Inferred from direct assay PubMed 10942595. Source: UniProtKB
Molecular_function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Arhgef7	Q9ES28	2	EBI-1046878,EBI-642580	From a different organism.
IKBKG	Q9Y6K9	6	EBI-1046878,EBI-81279
TNIP1	Q15025	3	EBI-1046878,EBI-357849
TRAF1	Q13077	2	EBI-1046878,EBI-359224
TSN	Q15631	2	EBI-1046878,EBI-1044160

Alternative products

This entry describes 11 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q14161-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14161-2)

Also known as: GIT2-short;

The sequence of this isoform differs from the canonical sequence as follows:
     466-471: QTLQSE → LGKDAN
     472-759: Missing.

Isoform 3 (identifier: Q14161-3)

Also known as: C-;

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.

Isoform 4 (identifier: Q14161-4)

Also known as: BC-;

The sequence of this isoform differs from the canonical sequence as follows:
     415-449: Missing.
     450-464: Missing.

Isoform 5 (identifier: Q14161-5)

Also known as: E-;

The sequence of this isoform differs from the canonical sequence as follows:
     548-577: Missing.

Isoform 6 (identifier: Q14161-6)

Also known as: CD-;

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.
     465-547: Missing.

Isoform 7 (identifier: Q14161-7)

Also known as: DE-;

The sequence of this isoform differs from the canonical sequence as follows:
     465-547: Missing.
     548-577: Missing.

Isoform 8 (identifier: Q14161-8)

Also known as: BE-;

The sequence of this isoform differs from the canonical sequence as follows:
     415-449: Missing.
     548-577: Missing.

Isoform 9 (identifier: Q14161-9)

Also known as: AE-;

The sequence of this isoform differs from the canonical sequence as follows:
     334-414: Missing.
     548-577: Missing.

Isoform 10 (identifier: Q14161-10)

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: S → RRL
     414-463: Missing.
     548-577: Missing.

Note: No experimental confirmation available.

Isoform 11 (identifier: Q14161-11)

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.
     465-547: Missing.
     548-577: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	ARF GTPase-activating protein GIT2		PRO_0000074203
Regions
	Domain	1 – 124	124	Arf-GAP
	Repeat	132 – 161	30	ANK 1
	Repeat	166 – 195	30	ANK 2
	Repeat	199 – 228	30	ANK 3
	Zinc finger	11 – 34	24	C4-type
Amino acid modifications
	Modified residue	394	1	Phosphoserine Ref.11 Ref.12
	Modified residue	397	1	Phosphoserine Ref.11 Ref.12
	Modified residue	401	1	Phosphothreonine By similarity
	Modified residue	415	1	Phosphoserine Ref.9
	Modified residue	418	1	Phosphoserine Ref.9
	Modified residue	421	1	Phosphoserine Ref.9 Ref.13
	Modified residue	484	1	Phosphotyrosine Ref.8
	Modified residue	559	1	Phosphoserine Ref.12
	Modified residue	586	1	Phosphoserine By similarity
	Modified residue	614	1	Phosphoserine Ref.10 Ref.12
Natural variations
	Alternative sequence	255	1	S → RRL in isoform 10.		VSP_026456
	Alternative sequence	334 – 414	81	Missing in isoform 9.		VSP_000305
	Alternative sequence	414 – 463	50	Missing in isoform 10.		VSP_008654
	Alternative sequence	415 – 449	35	Missing in isoform 4 and isoform 8.		VSP_000306
	Alternative sequence	450 – 464	15	Missing in isoform 3, isoform 4, isoform 6 and isoform 11.		VSP_000307
	Alternative sequence	465 – 547	83	Missing in isoform 6, isoform 7 and isoform 11.		VSP_000308
	Alternative sequence	466 – 471	6	QTLQSE → LGKDAN in isoform 2.		VSP_000303
	Alternative sequence	472 – 759	288	Missing in isoform 2.		VSP_000304
	Alternative sequence	548 – 577	30	Missing in isoform 5, isoform 7, isoform 8, isoform 9, isoform 10 and isoform 11.		VSP_000309
	Natural variant	338	1	N → S. Corresponds to variant rs9804905 [ dbSNP | Ensembl ].		VAR_048324
	Natural variant	387	1	N → S. Corresponds to variant rs925368 [ dbSNP | Ensembl ].		VAR_024368
	Natural variant	552	1	A → V. Corresponds to variant rs11068997 [ dbSNP | Ensembl ].		VAR_048325
Experimental info
	Sequence conflict	285	1	V → M in AAP35976. Ref.4
	Sequence conflict	285	1	V → M in AAH01379. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 18, 2001. Version 2. Checksum: 07EFE266DB2F3258	FASTA	759	84,543
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLQTLQS ENSNLRKQAT 490 500 510 520 530 540 TNVYQVQTGS EYTDTSNHSS LKRRPSARGS RPMSMYETGS GQKPYLPMGE ASRPEESRMR 550 560 570 580 590 600 LQPFPAHIGR SALVTSSSSL PSFPSTLSWS RDESARRASR LEKQNSTPES DYDNTPNDME 610 620 630 640 650 660 PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH VAPSPTLPST EDVIRKTEQI TKNIQELLRA 670 680 690 700 710 720 AQENKHDSYI PCSERIHVAV TEMAALFPKK PKSDMVRTSL RLLTSSAYRL QSECKKTLPG 730 740 750 DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV TITTKENNN
	Isoform 2 (GIT2-short) [UniParc]. Checksum: 4C82BB94EA3634DA	FASTA	471	52,577
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLLGKDA N
	Isoform 3 (C-) [UniParc]. Checksum: FE6EB7D7BA75DF1D	FASTA	744	82,759
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKL QTLQSENSNL RKQATTNVYQ VQTGSEYTDT 490 500 510 520 530 540 SNHSSLKRRP SARGSRPMSM YETGSGQKPY LPMGEASRPE ESRMRLQPFP AHIGRSALVT 550 560 570 580 590 600 SSSSLPSFPS TLSWSRDESA RRASRLEKQN STPESDYDNT PNDMEPDGMG SSRKGRQRSM 610 620 630 640 650 660 VWPGDGLVPD TAEPHVAPSP TLPSTEDVIR KTEQITKNIQ ELLRAAQENK HDSYIPCSER 670 680 690 700 710 720 IHVAVTEMAA LFPKKPKSDM VRTSLRLLTS SAYRLQSECK KTLPGDPGSP TDVQLVTQQV 730 740 IQCAYDIAKA AKQLVTITTK ENNN
	Isoform 4 (BC-) [UniParc]. Checksum: 5D6679379A84F684	FASTA	709	78,954
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKLQTLQS 430 440 450 460 470 480 ENSNLRKQAT TNVYQVQTGS EYTDTSNHSS LKRRPSARGS RPMSMYETGS GQKPYLPMGE 490 500 510 520 530 540 ASRPEESRMR LQPFPAHIGR SALVTSSSSL PSFPSTLSWS RDESARRASR LEKQNSTPES 550 560 570 580 590 600 DYDNTPNDME PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH VAPSPTLPST EDVIRKTEQI 610 620 630 640 650 660 TKNIQELLRA AQENKHDSYI PCSERIHVAV TEMAALFPKK PKSDMVRTSL RLLTSSAYRL 670 680 690 700 QSECKKTLPG DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV TITTKENNN
	Isoform 5 (E-) [UniParc]. Checksum: 6B3A644B9F357C11	FASTA	729	81,322
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLQTLQS ENSNLRKQAT 490 500 510 520 530 540 TNVYQVQTGS EYTDTSNHSS LKRRPSARGS RPMSMYETGS GQKPYLPMGE ASRPEESRMR 550 560 570 580 590 600 LQPFPAHASR LEKQNSTPES DYDNTPNDME PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH 610 620 630 640 650 660 VAPSPTLPST EDVIRKTEQI TKNIQELLRA AQENKHDSYI PCSERIHVAV TEMAALFPKK 670 680 690 700 710 720 PKSDMVRTSL RLLTSSAYRL QSECKKTLPG DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV TITTKENNN
	Isoform 6 (CD-) [UniParc]. Checksum: DD63B22D57DE9FAA	FASTA	661	73,403
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKI GRSALVTSSS SLPSFPSTLS WSRDESARRA 490 500 510 520 530 540 SRLEKQNSTP ESDYDNTPND MEPDGMGSSR KGRQRSMVWP GDGLVPDTAE PHVAPSPTLP 550 560 570 580 590 600 STEDVIRKTE QITKNIQELL RAAQENKHDS YIPCSERIHV AVTEMAALFP KKPKSDMVRT 610 620 630 640 650 660 SLRLLTSSAY RLQSECKKTL PGDPGSPTDV QLVTQQVIQC AYDIAKAAKQ LVTITTKENN N
	Isoform 7 (DE-) [UniParc]. Checksum: 5A146DE59620E062	FASTA	646	71,967
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKASRLEK QNSTPESDYD 490 500 510 520 530 540 NTPNDMEPDG MGSSRKGRQR SMVWPGDGLV PDTAEPHVAP SPTLPSTEDV IRKTEQITKN 550 560 570 580 590 600 IQELLRAAQE NKHDSYIPCS ERIHVAVTEM AALFPKKPKS DMVRTSLRLL TSSAYRLQSE 610 620 630 640 CKKTLPGDPG SPTDVQLVTQ QVIQCAYDIA KAAKQLVTIT TKENNN
	Isoform 8 (BE-) [UniParc]. Checksum: 40D620FE62406931	FASTA	694	77,518
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKVNNNLS 430 440 450 460 470 480 DELRIMQKKL QTLQSENSNL RKQATTNVYQ VQTGSEYTDT SNHSSLKRRP SARGSRPMSM 490 500 510 520 530 540 YETGSGQKPY LPMGEASRPE ESRMRLQPFP AHASRLEKQN STPESDYDNT PNDMEPDGMG 550 560 570 580 590 600 SSRKGRQRSM VWPGDGLVPD TAEPHVAPSP TLPSTEDVIR KTEQITKNIQ ELLRAAQENK 610 620 630 640 650 660 HDSYIPCSER IHVAVTEMAA LFPKKPKSDM VRTSLRLLTS SAYRLQSECK KTLPGDPGSP 670 680 690 TDVQLVTQQV IQCAYDIAKA AKQLVTITTK ENNN
	Isoform 9 (AE-) [UniParc]. Checksum: 3FE0E683427F3E09	FASTA	648	72,318
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKSLDSDLS DGPVTVQEFM EVKNALVASE 370 380 390 400 410 420 AKIQQLMKVN NNLSDELRIM QKKLQTLQSE NSNLRKQATT NVYQVQTGSE YTDTSNHSSL 430 440 450 460 470 480 KRRPSARGSR PMSMYETGSG QKPYLPMGEA SRPEESRMRL QPFPAHASRL EKQNSTPESD 490 500 510 520 530 540 YDNTPNDMEP DGMGSSRKGR QRSMVWPGDG LVPDTAEPHV APSPTLPSTE DVIRKTEQIT 550 560 570 580 590 600 KNIQELLRAA QENKHDSYIP CSERIHVAVT EMAALFPKKP KSDMVRTSLR LLTSSAYRLQ 610 620 630 640 SECKKTLPGD PGSPTDVQLV TQQVIQCAYD IAKAAKQLVT ITTKENNN
	Isoform 10 [UniParc]. Checksum: DADC7AB863457F3D	FASTA	681	76,072
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADRRLSLD LSELAKAAKK KLQSLSNHLF EELAMDVYDE VDRRETDAVW 310 320 330 340 350 360 LATQNHSALV TETTVVPFLP VNPEYSSTRN QGRQKLARFN AHEFATLVID ILSDAKRRQQ 370 380 390 400 410 420 GSSLSGSKDN VELILKTINN QHSVESQDND QPDYDSVASD EDTDLETTAS KTNRQKLQTL 430 440 450 460 470 480 QSENSNLRKQ ATTNVYQVQT GSEYTDTSNH SSLKRRPSAR GSRPMSMYET GSGQKPYLPM 490 500 510 520 530 540 GEASRPEESR MRLQPFPAHA SRLEKQNSTP ESDYDNTPND MEPDGMGSSR KGRQRSMVWP 550 560 570 580 590 600 GDGLVPDTAE PHVAPSPTLP STEDVIRKTE QITKNIQELL RAAQENKHDS YIPCSERIHV 610 620 630 640 650 660 AVTEMAALFP KKPKSDMVRT SLRLLTSSAY RLQSECKKTL PGDPGSPTDV QLVTQQVIQC 670 680 AYDIAKAAKQ LVTITTKENN N
	Isoform 11 [UniParc]. Checksum: C14DEB3FF1A577CD	FASTA	631	70,183
	10 20 30 40 50 60 MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA 310 320 330 340 350 360 TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS 370 380 390 400 410 420 SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL 430 440 450 460 470 480 SDGPVTVQEF MEVKNALVAS EAKIQQLMKA SRLEKQNSTP ESDYDNTPND MEPDGMGSSR 490 500 510 520 530 540 KGRQRSMVWP GDGLVPDTAE PHVAPSPTLP STEDVIRKTE QITKNIQELL RAAQENKHDS 550 560 570 580 590 600 YIPCSERIHV AVTEMAALFP KKPKSDMVRT SLRLLTSSAY RLQSECKKTL PGDPGSPTDV 610 620 630 QLVTQQVIQC AYDIAKAAKQ LVTITTKENN N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing." Premont R.T., Claing A., Vitale N., Perry S.J., Lefkowitz R.J. J. Biol. Chem. 275:22373-22380(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5; 6; 7; 8 AND 9), CHARACTERIZATION.
[2]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 10). Tissue: Bone marrow.
[3]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 29-759 (ISOFORM 11). Tissue: B-cell and Skin.
[6]	"An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization." Mazaki Y., Hashimoto S., Okawa K., Tsubouchi A., Nakamura K., Yagi R., Yano H., Kondo A., Iwamatsu A., Mizoguchi A., Sabe H. Mol. Biol. Cell 12:645-662(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PAXILLIN.
[7]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[8]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-484, MASS SPECTROMETRY.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-415; SER-418 AND SER-421, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394 AND SER-397, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394; SER-397; SER-559 AND SER-614, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]	"Bin2 is a membrane sculpting N-BAR protein that influences leucocyte podosomes, motility and phagocytosis." Sanchez-Barrena M.J., Vallis Y., Clatworthy M.R., Doherty G.J., Veprintsev D.B., Evans P.R., McMahon H.T. PLoS ONE 7:E52401-E52401(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH BIN2 AND ARHGEF6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF124491 mRNA. Translation: AAD28047.1. D63482 mRNA. Translation: BAA09769.2. Different initiation. BT007312 mRNA. Translation: AAP35976.1. BC001379 mRNA. Translation: AAH01379.1. BC014223 mRNA. Translation: AAH14223.2.
IPI	IPI00186690. IPI00216991. IPI00216993. IPI00216994. IPI00216996. IPI00216997. IPI00216998. IPI00238218. IPI00376496. IPI00413167. IPI00413822.
RefSeq	NP_001128685.1. NM_001135213.1. NP_001128686.1. NM_001135214.1. NP_055591.2. NM_014776.3. NP_476510.1. NM_057169.3. NP_476511.1. NM_057170.3.
UniGene	Hs.434996.
3D structure databases
ProteinModelPortal	Q14161.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14161. 40 interactions.
MINT	MINT-1953372.
STRING	9606.ENSP00000347464.
PTM databases
PhosphoSite	Q14161.
Polymorphism databases
DMDM	17376322.
Proteomic databases
PaxDb	Q14161.
PRIDE	Q14161.
Protocols and materials databases
DNASU	9815.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338373; ENSP00000340342; ENSG00000139436. ENST00000355312; ENSP00000347464; ENSG00000139436. ENST00000356259; ENSP00000348595; ENSG00000139436. ENST00000360185; ENSP00000353312; ENSG00000139436. ENST00000361006; ENSP00000354282; ENSG00000139436. ENST00000457474; ENSP00000391813; ENSG00000139436. ENST00000547815; ENSP00000450348; ENSG00000139436. ENST00000553118; ENSP00000447465; ENSG00000139436.
GeneID	9815.
KEGG	hsa:9815.
UCSC	uc001tpq.2. human. uc001tps.2. human. uc001tpt.2. human. uc001tpv.2. human.
Organism-specific databases
CTD	9815.
GeneCards	GC12M110367.
HGNC	HGNC:4273. GIT2.
HPA	CAB004681.
MIM	608564. gene.
neXtProt	NX_Q14161.
PharmGKB	PA28684.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5347.
HOGENOM	HOG000232135.
HOVERGEN	HBG012506.
InParanoid	Q14161.
KO	K12487.
OMA	GPDPSWA.
PhylomeDB	Q14161.
Gene expression databases
ArrayExpress	Q14161.
Bgee	Q14161.
CleanEx	HS_GIT2.
Genevestigator	Q14161.
GermOnline	ENSG00000139436. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 2 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001164. ArfGAP. IPR022018. GIT1_C. IPR013724. GIT_SHD. [Graphical view]
Pfam	PF00023. Ank. 1 hit. PF01412. ArfGap. 1 hit. PF12205. GIT1_C. 1 hit. PF08518. GIT_SHD. 2 hits. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00248. ANK. 3 hits. SM00105. ArfGap. 1 hit. SM00555. GIT. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS50115. ARFGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GIT2. human.
GenomeRNAi	9815.
NextBio	36954.
SOURCE	Search...

Entry information

Entry name

GIT2_HUMAN

Accession

Primary (citable) accession number: Q14161
Secondary accession number(s): Q86U59 , Q96CI2, Q9BV91, Q9Y5V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 18, 2001
Last modified:	May 1, 2013
This is version 141 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.