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Protein

ARF GTPase-activating protein GIT2

Gene

GIT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000139436-MONOMER.
SIGNORiQ14161.

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT2
Short name:
ARF GAP GIT2
Alternative name(s):
Cool-interacting tyrosine-phosphorylated protein 2
Short name:
CAT-2
Short name:
CAT2
G protein-coupled receptor kinase-interactor 2
GRK-interacting protein 2
Gene namesi
Name:GIT2
Synonyms:KIAA0148
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:4273. GIT2.

Subcellular locationi

GO - Cellular componenti

  • focal adhesion Source: UniProtKB
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi9815.
OpenTargetsiENSG00000139436.
PharmGKBiPA28684.

Polymorphism and mutation databases

BioMutaiGIT2.
DMDMi17376322.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742031 – 759ARF GTPase-activating protein GIT2Add BLAST759

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineBy similarity1
Modified residuei361PhosphoserineBy similarity1
Modified residuei384PhosphoserineBy similarity1
Modified residuei392PhosphotyrosineBy similarity1
Modified residuei394PhosphoserineCombined sources1
Modified residuei397PhosphoserineCombined sources1
Modified residuei401PhosphothreonineBy similarity1
Modified residuei415PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei421PhosphoserineCombined sources1
Modified residuei484PhosphotyrosineCombined sources1
Modified residuei488PhosphothreonineBy similarity1
Modified residuei506PhosphoserineBy similarity1
Modified residuei556PhosphoserineBy similarity1
Modified residuei559PhosphoserineCombined sources1
Modified residuei562PhosphoserineBy similarity1
Modified residuei570PhosphoserineBy similarity1
Modified residuei586PhosphoserineBy similarity1
Modified residuei587PhosphothreonineBy similarity1
Modified residuei590PhosphoserineBy similarity1
Modified residuei595PhosphothreonineBy similarity1
Modified residuei614PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14161.
MaxQBiQ14161.
PaxDbiQ14161.
PeptideAtlasiQ14161.
PRIDEiQ14161.

PTM databases

iPTMnetiQ14161.
PhosphoSitePlusiQ14161.

Expressioni

Gene expression databases

BgeeiENSG00000139436.
CleanExiHS_GIT2.
ExpressionAtlasiQ14161. baseline and differential.
GenevisibleiQ14161. HS.

Organism-specific databases

HPAiCAB004681.

Interactioni

Subunit structurei

Interacts with TGFB1I1 (By similarity). Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Identified in a complex with ARHGEF6 and BIN2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF6Q150524EBI-12028686,EBI-1642523
Arhgef7Q9ES282EBI-1046878,EBI-642580From a different organism.
IKBKGQ9Y6K96EBI-1046878,EBI-81279
PXNP490232EBI-1046878,EBI-702209
TNIP1Q150253EBI-1046878,EBI-357849
TRAF1Q130772EBI-1046878,EBI-359224
TSNQ156312EBI-1046878,EBI-1044160

Protein-protein interaction databases

BioGridi115154. 60 interactors.
IntActiQ14161. 47 interactors.
MINTiMINT-1953372.
STRINGi9606.ENSP00000347464.

Structurei

3D structure databases

ProteinModelPortaliQ14161.
SMRiQ14161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati132 – 161ANK 1Add BLAST30
Repeati166 – 195ANK 2Add BLAST30
Repeati199 – 228ANK 3Add BLAST30

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ14161.
KOiK12487.
OMAiRQGGHHE.
OrthoDBiEOG091G03VD.
PhylomeDBiQ14161.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR032352. GIT1/2_CC.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF16559. GIT_CC. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (11)i

Sequence statusi: Complete.

This entry describes 11 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q14161-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH
60 70 80 90 100
LKHTPWPPTL LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH
110 120 130 140 150
PNKAEFIRAK YQMLAFVHRL PCRDDDSVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGNTP LHVASKAGQI LQAELLAVYG ADPGTQDSSG
210 220 230 240 250
KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD HKNGQHFIIP
260 270 280 290 300
QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA
310 320 330 340 350
TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL
360 370 380 390 400
SDAKRRQQGS SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED
410 420 430 440 450
TDLETTASKT NRQKSLDSDL SDGPVTVQEF MEVKNALVAS EAKIQQLMKV
460 470 480 490 500
NNNLSDELRI MQKKLQTLQS ENSNLRKQAT TNVYQVQTGS EYTDTSNHSS
510 520 530 540 550
LKRRPSARGS RPMSMYETGS GQKPYLPMGE ASRPEESRMR LQPFPAHIGR
560 570 580 590 600
SALVTSSSSL PSFPSTLSWS RDESARRASR LEKQNSTPES DYDNTPNDME
610 620 630 640 650
PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH VAPSPTLPST EDVIRKTEQI
660 670 680 690 700
TKNIQELLRA AQENKHDSYI PCSERIHVAV TEMAALFPKK PKSDMVRTSL
710 720 730 740 750
RLLTSSAYRL QSECKKTLPG DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV

TITTKENNN
Length:759
Mass (Da):84,543
Last modified:October 18, 2001 - v2
Checksum:i07EFE266DB2F3258
GO
Isoform 2 (identifier: Q14161-2) [UniParc]FASTAAdd to basket
Also known as: GIT2-short

The sequence of this isoform differs from the canonical sequence as follows:
     466-471: QTLQSE → LGKDAN
     472-759: Missing.

Show »
Length:471
Mass (Da):52,577
Checksum:i4C82BB94EA3634DA
GO
Isoform 3 (identifier: Q14161-3) [UniParc]FASTAAdd to basket
Also known as: C-

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.

Show »
Length:744
Mass (Da):82,759
Checksum:iFE6EB7D7BA75DF1D
GO
Isoform 4 (identifier: Q14161-4) [UniParc]FASTAAdd to basket
Also known as: BC-

The sequence of this isoform differs from the canonical sequence as follows:
     415-449: Missing.
     450-464: Missing.

Show »
Length:709
Mass (Da):78,954
Checksum:i5D6679379A84F684
GO
Isoform 5 (identifier: Q14161-5) [UniParc]FASTAAdd to basket
Also known as: E-

The sequence of this isoform differs from the canonical sequence as follows:
     548-577: Missing.

Show »
Length:729
Mass (Da):81,322
Checksum:i6B3A644B9F357C11
GO
Isoform 6 (identifier: Q14161-6) [UniParc]FASTAAdd to basket
Also known as: CD-

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.
     465-547: Missing.

Show »
Length:661
Mass (Da):73,403
Checksum:iDD63B22D57DE9FAA
GO
Isoform 7 (identifier: Q14161-7) [UniParc]FASTAAdd to basket
Also known as: DE-

The sequence of this isoform differs from the canonical sequence as follows:
     465-547: Missing.
     548-577: Missing.

Show »
Length:646
Mass (Da):71,967
Checksum:i5A146DE59620E062
GO
Isoform 8 (identifier: Q14161-8) [UniParc]FASTAAdd to basket
Also known as: BE-

The sequence of this isoform differs from the canonical sequence as follows:
     415-449: Missing.
     548-577: Missing.

Show »
Length:694
Mass (Da):77,518
Checksum:i40D620FE62406931
GO
Isoform 9 (identifier: Q14161-9) [UniParc]FASTAAdd to basket
Also known as: AE-

The sequence of this isoform differs from the canonical sequence as follows:
     334-414: Missing.
     548-577: Missing.

Show »
Length:648
Mass (Da):72,318
Checksum:i3FE0E683427F3E09
GO
Isoform 10 (identifier: Q14161-10) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: S → RRL
     414-463: Missing.
     548-577: Missing.

Note: No experimental confirmation available.
Show »
Length:681
Mass (Da):76,072
Checksum:iDADC7AB863457F3D
GO
Isoform 11 (identifier: Q14161-11) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     450-464: Missing.
     465-547: Missing.
     548-577: Missing.

Note: No experimental confirmation available.
Show »
Length:631
Mass (Da):70,183
Checksum:iC14DEB3FF1A577CD
GO

Sequence cautioni

The sequence BAA09769 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285V → M in AAP35976 (Ref. 4) Curated1
Sequence conflicti285V → M in AAH01379 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048324338N → S.Corresponds to variant rs9804905dbSNPEnsembl.1
Natural variantiVAR_024368387N → S.Corresponds to variant rs925368dbSNPEnsembl.1
Natural variantiVAR_048325552A → V.Corresponds to variant rs11068997dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026456255S → RRL in isoform 10. 1 Publication1
Alternative sequenceiVSP_000305334 – 414Missing in isoform 9. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_008654414 – 463Missing in isoform 10. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_000306415 – 449Missing in isoform 4 and isoform 8. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_000307450 – 464Missing in isoform 3, isoform 4, isoform 6 and isoform 11. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_000308465 – 547Missing in isoform 6, isoform 7 and isoform 11. 2 PublicationsAdd BLAST83
Alternative sequenceiVSP_000303466 – 471QTLQSE → LGKDAN in isoform 2. 3 Publications6
Alternative sequenceiVSP_000304472 – 759Missing in isoform 2. 3 PublicationsAdd BLAST288
Alternative sequenceiVSP_000309548 – 577Missing in isoform 5, isoform 7, isoform 8, isoform 9, isoform 10 and isoform 11. 3 PublicationsAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124491 mRNA. Translation: AAD28047.1.
D63482 mRNA. Translation: BAA09769.2. Different initiation.
BT007312 mRNA. Translation: AAP35976.1.
BC001379 mRNA. Translation: AAH01379.1.
BC014223 mRNA. Translation: AAH14223.2.
CCDSiCCDS44968.1. [Q14161-5]
CCDS44969.1. [Q14161-11]
CCDS55884.1. [Q14161-10]
CCDS9138.1. [Q14161-1]
CCDS9139.1. [Q14161-2]
RefSeqiNP_001128685.1. NM_001135213.2. [Q14161-10]
NP_001128686.1. NM_001135214.2. [Q14161-5]
NP_001317082.1. NM_001330153.1.
NP_055591.2. NM_014776.4.
NP_476510.1. NM_057169.4. [Q14161-1]
NP_476511.1. NM_057170.4. [Q14161-11]
XP_006719770.1. XM_006719707.3. [Q14161-3]
XP_006719772.1. XM_006719709.3. [Q14161-4]
UniGeneiHs.434996.

Genome annotation databases

EnsembliENST00000355312; ENSP00000347464; ENSG00000139436. [Q14161-1]
ENST00000361006; ENSP00000354282; ENSG00000139436. [Q14161-5]
ENST00000457474; ENSP00000391813; ENSG00000139436. [Q14161-10]
ENST00000547815; ENSP00000450348; ENSG00000139436. [Q14161-2]
ENST00000553118; ENSP00000447465; ENSG00000139436. [Q14161-11]
GeneIDi9815.
KEGGihsa:9815.
UCSCiuc001tpq.3. human. [Q14161-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124491 mRNA. Translation: AAD28047.1.
D63482 mRNA. Translation: BAA09769.2. Different initiation.
BT007312 mRNA. Translation: AAP35976.1.
BC001379 mRNA. Translation: AAH01379.1.
BC014223 mRNA. Translation: AAH14223.2.
CCDSiCCDS44968.1. [Q14161-5]
CCDS44969.1. [Q14161-11]
CCDS55884.1. [Q14161-10]
CCDS9138.1. [Q14161-1]
CCDS9139.1. [Q14161-2]
RefSeqiNP_001128685.1. NM_001135213.2. [Q14161-10]
NP_001128686.1. NM_001135214.2. [Q14161-5]
NP_001317082.1. NM_001330153.1.
NP_055591.2. NM_014776.4.
NP_476510.1. NM_057169.4. [Q14161-1]
NP_476511.1. NM_057170.4. [Q14161-11]
XP_006719770.1. XM_006719707.3. [Q14161-3]
XP_006719772.1. XM_006719709.3. [Q14161-4]
UniGeneiHs.434996.

3D structure databases

ProteinModelPortaliQ14161.
SMRiQ14161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115154. 60 interactors.
IntActiQ14161. 47 interactors.
MINTiMINT-1953372.
STRINGi9606.ENSP00000347464.

PTM databases

iPTMnetiQ14161.
PhosphoSitePlusiQ14161.

Polymorphism and mutation databases

BioMutaiGIT2.
DMDMi17376322.

Proteomic databases

EPDiQ14161.
MaxQBiQ14161.
PaxDbiQ14161.
PeptideAtlasiQ14161.
PRIDEiQ14161.

Protocols and materials databases

DNASUi9815.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355312; ENSP00000347464; ENSG00000139436. [Q14161-1]
ENST00000361006; ENSP00000354282; ENSG00000139436. [Q14161-5]
ENST00000457474; ENSP00000391813; ENSG00000139436. [Q14161-10]
ENST00000547815; ENSP00000450348; ENSG00000139436. [Q14161-2]
ENST00000553118; ENSP00000447465; ENSG00000139436. [Q14161-11]
GeneIDi9815.
KEGGihsa:9815.
UCSCiuc001tpq.3. human. [Q14161-1]

Organism-specific databases

CTDi9815.
DisGeNETi9815.
GeneCardsiGIT2.
HGNCiHGNC:4273. GIT2.
HPAiCAB004681.
MIMi608564. gene.
neXtProtiNX_Q14161.
OpenTargetsiENSG00000139436.
PharmGKBiPA28684.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ14161.
KOiK12487.
OMAiRQGGHHE.
OrthoDBiEOG091G03VD.
PhylomeDBiQ14161.
TreeFamiTF317762.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000139436-MONOMER.
SIGNORiQ14161.

Miscellaneous databases

ChiTaRSiGIT2. human.
GeneWikiiGIT2.
GenomeRNAii9815.
PROiQ14161.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139436.
CleanExiHS_GIT2.
ExpressionAtlasiQ14161. baseline and differential.
GenevisibleiQ14161. HS.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR032352. GIT1/2_CC.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF16559. GIT_CC. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGIT2_HUMAN
AccessioniPrimary (citable) accession number: Q14161
Secondary accession number(s): Q86U59
, Q96CI2, Q9BV91, Q9Y5V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 18, 2001
Last modified: November 30, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.