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Protein

Tripartite motif-containing protein 14

Gene

TRIM14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways (PubMed:24379373). Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation (PubMed:27666593).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri19 – 61B box-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

GO - Biological processi

  • innate immune response Source: UniProtKB
  • negative regulation of viral transcription Source: UniProtKB
  • positive regulation of DNA binding transcription factor activity Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB

Keywordsi

Biological processImmunity, Innate immunity
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 14
Gene namesi
Name:TRIM14
Synonyms:KIAA0129
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000106785.14
HGNCiHGNC:16283 TRIM14
MIMi606556 gene
neXtProtiNX_Q14142

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi365K → R: More than 50% loss of interaction with MAVS. 1 Publication1

Organism-specific databases

DisGeNETi9830
OpenTargetsiENSG00000106785
PharmGKBiPA38113

Polymorphism and mutation databases

DMDMi20141957

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002203701 – 442Tripartite motif-containing protein 14Add BLAST442

Post-translational modificationi

Ubiquitinated (PubMed:24379373). Undergoes 'Lys-63'-linked polyubiquitination; this modification allows IKBKG/NEMO recruitment to MAVS.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ14142
MaxQBiQ14142
PaxDbiQ14142
PeptideAtlasiQ14142
PRIDEiQ14142
ProteomicsDBi59847
59848 [Q14142-2]

PTM databases

iPTMnetiQ14142
PhosphoSitePlusiQ14142

Expressioni

Tissue specificityi

Highest expression in liver; undetectable in skeletal muscle.

Inductioni

By interferons alpha and gamma.1 Publication

Gene expression databases

BgeeiENSG00000106785
CleanExiHS_TRIM14
ExpressionAtlasiQ14142 baseline and differential
GenevisibleiQ14142 HS

Organism-specific databases

HPAiHPA053217

Interactioni

Subunit structurei

Interacts with MAVS (PubMed:24379373). Interacts with WRNIP1 and PPP6C; these interactions positively regulate the RIG-I/DDX58 signaling pathway (PubMed:29053956). Interacts with CGAS; this interaction stabilizes CGAS and promotes type I interferon production (PubMed:27666593). Interacts with USP14; this interaction mediates the cleavage of 'Lys-48'-linked ubiquitination of CGAS (PubMed:27666593). Interacts with SPI1 (By similarity).By similarity3 Publications

Protein-protein interaction databases

BioGridi115168, 10 interactors
IntActiQ14142, 37 interactors
STRINGi9606.ENSP00000343990

Structurei

3D structure databases

ProteinModelPortaliQ14142
SMRiQ14142
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini249 – 442B30.2/SPRYPROSITE-ProRule annotationAdd BLAST194

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili76 – 109Sequence analysisAdd BLAST34
Coiled coili135 – 156Sequence analysisAdd BLAST22

Domaini

The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri19 – 61B box-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJ3M Eukaryota
ENOG410YJX9 LUCA
GeneTreeiENSGT00840000129835
HOGENOMiHOG000013201
HOVERGENiHBG054251
InParanoidiQ14142
KOiK12004
OMAiLEYWAFH
OrthoDBiEOG091G09TT
PhylomeDBiQ14142
TreeFamiTF351014

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR000315 Znf_B-box
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00589 PRY, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q14142-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGAATGSRT PGRSELVEGC GWRCPEHGDR VAELFCRRCR RCVCALCPVL
60 70 80 90 100
GAHRGHPVGL ALEAAVHVQK LSQECLKQLA IKKQQHIDNI TQIEDATEKL
110 120 130 140 150
KANAESSKTW LKGKFTELRL LLDEEEALAK KFIDKNTQLT LQVYREQADS
160 170 180 190 200
CREQLDIMND LSNRVWSISQ EPDPVQRLQA YTATEQEMQQ QMSLGELCHP
210 220 230 240 250
VPLSFEPVKS FFKGLVEAVE STLQTPLDIR LKESINCQLS DPSSTKPGTL
260 270 280 290 300
LKTSPSPERS LLLKYARTPT LDPDTMHARL RLSADRLTVR CGLLGSLGPV
310 320 330 340 350
PVLRFDALWQ VLARDCFATG RHYWEVDVQE AGAGWWVGAA YASLRRRGAS
360 370 380 390 400
AAARLGCNRQ SWCLKRYDLE YWAFHDGQRS RLRPRDDLDR LGVFLDYEAG
410 420 430 440
VLAFYDVTGG MSHLHTFRAT FQEPLYPALR LWEGAISIPR LP
Length:442
Mass (Da):49,773
Last modified:February 21, 2002 - v2
Checksum:i68990B2B57F1D083
GO
Isoform Beta (identifier: Q14142-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     232-253: KESINCQLSDPSSTKPGTLLKT → SPTNHAYSALKVSSPRLVSNRP
     254-442: Missing.

Show »
Length:253
Mass (Da):28,347
Checksum:iD3E44449E5113EAC
GO
Isoform 3 (identifier: Q14142-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-442: RLRPRDDLDR...EGAISIPRLP → ACGPATTSTGSASSWTTRPASSPSTT

Show »
Length:406
Mass (Da):45,112
Checksum:iE6E0BA0376315664
GO
Isoform 4 (identifier: Q14142-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.
     265-380: YARTPTLDPD...YWAFHDGQRS → SCWAAWGPCP...TGPSTTASAA
     381-442: RLRPRDDLDR...EGAISIPRLP → ACGPATTSTGSASSWTTRPASSPSTT

Show »
Length:222
Mass (Da):23,146
Checksum:i761C4E78AE472996
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 4 (identifier: Q14142-4)
Sequence conflicti106 – 108LKS → PIA in BAC05071 (PubMed:14702039).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048399219V → M. Corresponds to variant dbSNP:rs2296079Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0440971 – 157Missing in isoform 4. 1 PublicationAdd BLAST157
Alternative sequenceiVSP_000510232 – 253KESIN…TLLKT → SPTNHAYSALKVSSPRLVSN RP in isoform Beta. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_000511254 – 442Missing in isoform Beta. 1 PublicationAdd BLAST189
Alternative sequenceiVSP_044098265 – 380YARTP…DGQRS → SCWAAWGPCPCCGSTRSGKC WLVTASPPAATTGRLTCRRR APAGGWARPTPPFGAAGPRP PPAWAATASPGASSATTLST GPSTTASAA in isoform 4. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_044099381 – 442RLRPR…IPRLP → ACGPATTSTGSASSWTTRPA SSPSTT in isoform 3 and isoform 4. 3 PublicationsAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220130 mRNA Translation: AAG53503.1
AF220131 mRNA Translation: AAG53504.1
D50919 mRNA Translation: BAA09478.1
AK097480 mRNA Translation: BAC05071.1
AK292825 mRNA Translation: BAF85514.1
AL137073 Genomic DNA No translation available.
CH471105 Genomic DNA Translation: EAW58870.1
CH471105 Genomic DNA Translation: EAW58877.1
BC006333 mRNA Translation: AAH06333.1
CCDSiCCDS6734.1 [Q14142-1]
RefSeqiNP_055603.2, NM_014788.3 [Q14142-1]
NP_150088.1, NM_033219.2 [Q14142-1]
XP_005252377.1, XM_005252320.3 [Q14142-1]
XP_016870841.1, XM_017015352.1 [Q14142-1]
XP_016870842.1, XM_017015353.1 [Q14142-1]
UniGeneiHs.575631
Hs.665191

Genome annotation databases

EnsembliENST00000341469; ENSP00000344208; ENSG00000106785 [Q14142-1]
ENST00000342043; ENSP00000343990; ENSG00000106785 [Q14142-1]
ENST00000375098; ENSP00000364239; ENSG00000106785 [Q14142-1]
GeneIDi9830
KEGGihsa:9830
UCSCiuc004ayg.3 human [Q14142-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTRI14_HUMAN
AccessioniPrimary (citable) accession number: Q14142
Secondary accession number(s): A8K9W0
, E7EQC4, F8W956, Q548W9, Q5TBQ8, Q6ZWL7, Q9BRD8, Q9C020
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 21, 2002
Last modified: June 20, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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