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Protein

Ubiquitin conjugation factor E4 A

Gene

UBE4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 ACurated (EC:2.3.2.27By similarity)
Alternative name(s):
RING-type E3 ubiquitin transferase E4 A
Gene namesi
Name:UBE4AImported
Synonyms:KIAA0126Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110344.9
HGNCiHGNC:12499 UBE4A
MIMi603753 gene
neXtProtiNX_Q14139

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi9354
OpenTargetsiENSG00000110344
PharmGKBiPA37147

Polymorphism and mutation databases

BioMutaiUBE4A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949901 – 1066Ubiquitin conjugation factor E4 AAdd BLAST1066

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ14139
MaxQBiQ14139
PaxDbiQ14139
PeptideAtlasiQ14139
PRIDEiQ14139

PTM databases

iPTMnetiQ14139
PhosphoSitePlusiQ14139

Miscellaneous databases

PMAP-CutDBiB0YJB6

Expressioni

Gene expression databases

BgeeiENSG00000110344
CleanExiHS_UBE4A
ExpressionAtlasiQ14139 baseline and differential
GenevisibleiQ14139 HS

Organism-specific databases

HPAiHPA055035
HPA064010

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MAVSQ7Z4342EBI-1048119,EBI-995373

Protein-protein interaction databases

BioGridi114757, 40 interactors
IntActiQ14139, 13 interactors
MINTiQ14139
STRINGi9606.ENSP00000387362

Structurei

Secondary structure

11066
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni990 – 992Combined sources3
Turni995 – 997Combined sources3
Beta strandi1002 – 1006Combined sources5
Turni1008 – 1010Combined sources3
Beta strandi1013 – 1015Combined sources3
Helixi1016 – 1022Combined sources7
Turni1023 – 1025Combined sources3
Turni1030 – 1032Combined sources3
Turni1038 – 1040Combined sources3
Beta strandi1041 – 1043Combined sources3
Helixi1045 – 1057Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGMNMR-A977-1061[»]
ProteinModelPortaliQ14139
SMRiQ14139
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14139

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini987 – 1061U-boxAdd BLAST75

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2042 Eukaryota
COG5113 LUCA
GeneTreeiENSGT00390000009300
HOGENOMiHOG000007049
HOVERGENiHBG059871
InParanoidiQ14139
KOiK10596
OMAiTLHLGFH
OrthoDBiEOG091G07J9
PhylomeDBiQ14139
TreeFamiTF300802

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR019474 Ub_conjug_fac_E4_core
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
PF10408 Ufd2P_core, 1 hit
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS
60 70 80 90 100
DNSVSESLDE FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD
110 120 130 140 150
PSLKSGNGIP SRCVYLEEMA VELEDQDWLD MSNVEQALFA RLLLQDPGNH
160 170 180 190 200
LINMTSSTTL NLSADRDAGE RHIFCYLYSC FQRAKEEITK VPENLLPFAV
210 220 230 240 250
QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA HFEDVTEFLE
260 270 280 290 300
EVIEALILDE EVRTFPEVMI PVFDILLGRI KDLELCQILL YAYLDILLYF
310 320 330 340 350
TRQKDMAKVF VEYIQPKDPT NGQMYQKTLL GVILSISCLL KTPGVVENHG
360 370 380 390 400
YFLNPSRSSP QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL
410 420 430 440 450
SWLGNCLHAN AGRTKIWANQ MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC
460 470 480 490 500
KPRSSRLLTF NPTYCALKEL NDEERKIKNV HMRGLDKETC LIPAVQEPKF
510 520 530 540 550
PQNYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ VAWRDAQQSS
560 570 580 590 600
SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI
610 620 630 640 650
GNEGSQPIEL TFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFADDILETS
660 670 680 690 700
ADSLEHVLHF ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPNPLVS
710 720 730 740 750
SVFHRKRVFC NFQYAPQLAE ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP
760 770 780 790 800
ILRYMWGTDT YRESIKDLAD YASKNLEAMN PPLFLRFLNL LMNDAIFLLD
810 820 830 840 850
EAIQYLSKIK IQQIEKDRGE WDSLTPEARR EKEAGLQMFG QLARFHNIMS
860 870 880 890 900
NETIGTLAFL TSEIKSLFVH PFLAERIISM LNYFLQHLVG PKMGALKVKD
910 920 930 940 950
FSEFDFKPQQ LVSDICTIYL NLGDEENFCA TVPKDGRSYS PTLFAQTVRV
960 970 980 990 1000
LKKINKPGNM IMAFSNLAER IKSLADLQQQ EEETYADACD EFLDPIMSTL
1010 1020 1030 1040 1050
MCDPVVLPSS RVTVDRSTIA RHLLSDQTDP FNRSPLTMDQ IRPNTELKEK
1060
IQRWLAERKQ QKEQLE
Length:1,066
Mass (Da):122,561
Last modified:May 10, 2005 - v2
Checksum:iA735D670220F9DDA
GO
Isoform 2 (identifier: Q14139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-241: H → REYMNKIY

Note: No experimental confirmation available.
Show »
Length:1,073
Mass (Da):123,522
Checksum:i11AC6B62DD4076E7
GO

Sequence cautioni

The sequence AAH52643 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH62695 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA09475 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217P → L in BAA09475 (PubMed:8590280).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013673241H → REYMNKIY in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50916 mRNA Translation: BAA09475.2 Different initiation.
EF445042 Genomic DNA Translation: ACA06094.1
CH471065 Genomic DNA Translation: EAW67371.1
BC052643 mRNA Translation: AAH52643.1 Sequence problems.
BC062695 mRNA Translation: AAH62695.1 Sequence problems.
BC111417 mRNA Translation: AAI11418.1
BC112367 mRNA Translation: AAI12368.1
CCDSiCCDS55790.1 [Q14139-1]
CCDS8396.1 [Q14139-2]
RefSeqiNP_001191006.1, NM_001204077.1 [Q14139-1]
NP_004779.2, NM_004788.3 [Q14139-2]
UniGeneiHs.602967
Hs.75275

Genome annotation databases

EnsembliENST00000252108; ENSP00000252108; ENSG00000110344 [Q14139-1]
ENST00000431736; ENSP00000387362; ENSG00000110344 [Q14139-2]
GeneIDi9354
KEGGihsa:9354
UCSCiuc001psv.4 human [Q14139-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBE4A_HUMAN
AccessioniPrimary (citable) accession number: Q14139
Secondary accession number(s): B0YJB6
, Q2M1H0, Q6P5T4, Q7Z639
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 10, 2005
Last modified: April 25, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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