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Protein

Ubiquitin conjugation factor E4 A

Gene

UBE4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein polyubiquitination Source: Ensembl
  • ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 ACurated (EC:6.3.2.-By similarity)
Gene namesi
Name:UBE4AImported
Synonyms:KIAA0126Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:12499. UBE4A.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • ubiquitin ligase complex Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37147.

Polymorphism and mutation databases

BioMutaiUBE4A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Ubiquitin conjugation factor E4 APRO_0000194990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei386 – 3861N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ14139.
MaxQBiQ14139.
PaxDbiQ14139.
PeptideAtlasiQ14139.
PRIDEiQ14139.

PTM databases

iPTMnetiQ14139.
PhosphoSiteiQ14139.

Miscellaneous databases

PMAP-CutDBB0YJB6.

Expressioni

Gene expression databases

BgeeiQ14139.
CleanExiHS_UBE4A.
ExpressionAtlasiQ14139. baseline and differential.
GenevisibleiQ14139. HS.

Organism-specific databases

HPAiHPA055035.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MAVSQ7Z4342EBI-1048119,EBI-995373

Protein-protein interaction databases

BioGridi114757. 33 interactions.
IntActiQ14139. 11 interactions.
STRINGi9606.ENSP00000387362.

Structurei

Secondary structure

1
1066
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni990 – 9923Combined sources
Turni995 – 9973Combined sources
Beta strandi1002 – 10065Combined sources
Turni1008 – 10103Combined sources
Beta strandi1013 – 10153Combined sources
Helixi1016 – 10227Combined sources
Turni1023 – 10253Combined sources
Turni1030 – 10323Combined sources
Turni1038 – 10403Combined sources
Beta strandi1041 – 10433Combined sources
Helixi1045 – 105713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WGMNMR-A977-1061[»]
ProteinModelPortaliQ14139.
SMRiQ14139. Positions 977-1061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14139.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini987 – 106175U-boxAdd
BLAST

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.By similarity

Sequence similaritiesi

Contains 1 U-box domain.Curated

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
HOGENOMiHOG000007049.
HOVERGENiHBG059871.
InParanoidiQ14139.
KOiK10596.
OMAiHFEDVTE.
PhylomeDBiQ14139.
TreeFamiTF300802.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS
60 70 80 90 100
DNSVSESLDE FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD
110 120 130 140 150
PSLKSGNGIP SRCVYLEEMA VELEDQDWLD MSNVEQALFA RLLLQDPGNH
160 170 180 190 200
LINMTSSTTL NLSADRDAGE RHIFCYLYSC FQRAKEEITK VPENLLPFAV
210 220 230 240 250
QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA HFEDVTEFLE
260 270 280 290 300
EVIEALILDE EVRTFPEVMI PVFDILLGRI KDLELCQILL YAYLDILLYF
310 320 330 340 350
TRQKDMAKVF VEYIQPKDPT NGQMYQKTLL GVILSISCLL KTPGVVENHG
360 370 380 390 400
YFLNPSRSSP QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL
410 420 430 440 450
SWLGNCLHAN AGRTKIWANQ MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC
460 470 480 490 500
KPRSSRLLTF NPTYCALKEL NDEERKIKNV HMRGLDKETC LIPAVQEPKF
510 520 530 540 550
PQNYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ VAWRDAQQSS
560 570 580 590 600
SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI
610 620 630 640 650
GNEGSQPIEL TFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFADDILETS
660 670 680 690 700
ADSLEHVLHF ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPNPLVS
710 720 730 740 750
SVFHRKRVFC NFQYAPQLAE ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP
760 770 780 790 800
ILRYMWGTDT YRESIKDLAD YASKNLEAMN PPLFLRFLNL LMNDAIFLLD
810 820 830 840 850
EAIQYLSKIK IQQIEKDRGE WDSLTPEARR EKEAGLQMFG QLARFHNIMS
860 870 880 890 900
NETIGTLAFL TSEIKSLFVH PFLAERIISM LNYFLQHLVG PKMGALKVKD
910 920 930 940 950
FSEFDFKPQQ LVSDICTIYL NLGDEENFCA TVPKDGRSYS PTLFAQTVRV
960 970 980 990 1000
LKKINKPGNM IMAFSNLAER IKSLADLQQQ EEETYADACD EFLDPIMSTL
1010 1020 1030 1040 1050
MCDPVVLPSS RVTVDRSTIA RHLLSDQTDP FNRSPLTMDQ IRPNTELKEK
1060
IQRWLAERKQ QKEQLE
Length:1,066
Mass (Da):122,561
Last modified:May 10, 2005 - v2
Checksum:iA735D670220F9DDA
GO
Isoform 2 (identifier: Q14139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-241: H → REYMNKIY

Note: No experimental confirmation available.
Show »
Length:1,073
Mass (Da):123,522
Checksum:i11AC6B62DD4076E7
GO

Sequence cautioni

The sequence AAH52643.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH62695.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA09475.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti217 – 2171P → L in BAA09475 (PubMed:8590280).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei241 – 2411H → REYMNKIY in isoform 2. 1 PublicationVSP_013673

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50916 mRNA. Translation: BAA09475.2. Different initiation.
EF445042 Genomic DNA. Translation: ACA06094.1.
CH471065 Genomic DNA. Translation: EAW67371.1.
BC052643 mRNA. Translation: AAH52643.1. Sequence problems.
BC062695 mRNA. Translation: AAH62695.1. Sequence problems.
BC111417 mRNA. Translation: AAI11418.1.
BC112367 mRNA. Translation: AAI12368.1.
CCDSiCCDS55790.1. [Q14139-1]
CCDS8396.1. [Q14139-2]
RefSeqiNP_001191006.1. NM_001204077.1. [Q14139-1]
NP_004779.2. NM_004788.3. [Q14139-2]
UniGeneiHs.602967.
Hs.75275.

Genome annotation databases

EnsembliENST00000252108; ENSP00000252108; ENSG00000110344. [Q14139-1]
ENST00000431736; ENSP00000387362; ENSG00000110344. [Q14139-2]
GeneIDi9354.
KEGGihsa:9354.
UCSCiuc001psv.4. human. [Q14139-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50916 mRNA. Translation: BAA09475.2. Different initiation.
EF445042 Genomic DNA. Translation: ACA06094.1.
CH471065 Genomic DNA. Translation: EAW67371.1.
BC052643 mRNA. Translation: AAH52643.1. Sequence problems.
BC062695 mRNA. Translation: AAH62695.1. Sequence problems.
BC111417 mRNA. Translation: AAI11418.1.
BC112367 mRNA. Translation: AAI12368.1.
CCDSiCCDS55790.1. [Q14139-1]
CCDS8396.1. [Q14139-2]
RefSeqiNP_001191006.1. NM_001204077.1. [Q14139-1]
NP_004779.2. NM_004788.3. [Q14139-2]
UniGeneiHs.602967.
Hs.75275.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WGMNMR-A977-1061[»]
ProteinModelPortaliQ14139.
SMRiQ14139. Positions 977-1061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114757. 33 interactions.
IntActiQ14139. 11 interactions.
STRINGi9606.ENSP00000387362.

PTM databases

iPTMnetiQ14139.
PhosphoSiteiQ14139.

Polymorphism and mutation databases

BioMutaiUBE4A.

Proteomic databases

EPDiQ14139.
MaxQBiQ14139.
PaxDbiQ14139.
PeptideAtlasiQ14139.
PRIDEiQ14139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252108; ENSP00000252108; ENSG00000110344. [Q14139-1]
ENST00000431736; ENSP00000387362; ENSG00000110344. [Q14139-2]
GeneIDi9354.
KEGGihsa:9354.
UCSCiuc001psv.4. human. [Q14139-1]

Organism-specific databases

CTDi9354.
GeneCardsiUBE4A.
HGNCiHGNC:12499. UBE4A.
HPAiHPA055035.
MIMi603753. gene.
neXtProtiNX_Q14139.
PharmGKBiPA37147.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
HOGENOMiHOG000007049.
HOVERGENiHBG059871.
InParanoidiQ14139.
KOiK10596.
OMAiHFEDVTE.
PhylomeDBiQ14139.
TreeFamiTF300802.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiUBE4A. human.
EvolutionaryTraceiQ14139.
GeneWikiiUBE4A.
GenomeRNAii9354.
PMAP-CutDBB0YJB6.
PROiQ14139.
SOURCEiSearch...

Gene expression databases

BgeeiQ14139.
CleanExiHS_UBE4A.
ExpressionAtlasiQ14139. baseline and differential.
GenevisibleiQ14139. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."
    Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N.
    DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Bone marrow.
  2. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 545-558.
    Tissue: Platelet.
  6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-386, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Solution structure of the U-box in human ubiquitin conjugation factor E4A."
    RIKEN structural genomics initiative (RSGI)
    Submitted (MAY-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 977-1061.

Entry informationi

Entry nameiUBE4A_HUMAN
AccessioniPrimary (citable) accession number: Q14139
Secondary accession number(s): B0YJB6
, Q2M1H0, Q6P5T4, Q7Z639
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 10, 2005
Last modified: July 6, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.