Q14139 (UBE4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin conjugation factor E4 A

Gene names

Name:	UBE4A
Synonyms:	KIAA0126

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1066 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 By similarity.
Sequence similarities	Belongs to the ubiquitin conjugation factor E4 family. Contains 1 U-box domain.
Sequence caution	The sequence AAH52643.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH62695.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA09475.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Traceable author statement. Source: ProtInc
Cellular component	ubiquitin ligase complex Traceable author statement. Source: ProtInc
Molecular function	ubiquitin-ubiquitin ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14139-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14139-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-241: H → REYMNKIY

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1066

1066

Ubiquitin conjugation factor E4 A

PRO_0000194990

Regions

Domain

991 – 1054

64

U-box

Amino acid modifications

Modified residue

386

1

N6-acetyllysine Ref.6

Natural variations

Alternative sequence

241

1

H → REYMNKIY in isoform 2.

VSP_013673

Experimental info

Sequence conflict

217

1

P → L in BAA09475. Ref.1

Secondary structure

1

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1066

Helix Strand Turn

Details...

Turn

990 – 992

3

Turn

995 – 997

3

Beta strand

1002 – 1006

5

Turn

1008 – 1010

3

Beta strand

1013 – 1015

3

Helix

1016 – 1022

7

Turn

1023 – 1025

3

Turn

1030 – 1032

3

Turn

1038 – 1040

3

Beta strand

1041 – 1043

3

Helix

1045 – 1057

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: A735D670220F9DDA	FASTA	1,066	122,561
	10 20 30 40 50 60 MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE 70 80 90 100 110 120 FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD PSLKSGNGIP SRCVYLEEMA 130 140 150 160 170 180 VELEDQDWLD MSNVEQALFA RLLLQDPGNH LINMTSSTTL NLSADRDAGE RHIFCYLYSC 190 200 210 220 230 240 FQRAKEEITK VPENLLPFAV QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA 250 260 270 280 290 300 HFEDVTEFLE EVIEALILDE EVRTFPEVMI PVFDILLGRI KDLELCQILL YAYLDILLYF 310 320 330 340 350 360 TRQKDMAKVF VEYIQPKDPT NGQMYQKTLL GVILSISCLL KTPGVVENHG YFLNPSRSSP 370 380 390 400 410 420 QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL SWLGNCLHAN AGRTKIWANQ 430 440 450 460 470 480 MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC KPRSSRLLTF NPTYCALKEL NDEERKIKNV 490 500 510 520 530 540 HMRGLDKETC LIPAVQEPKF PQNYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ 550 560 570 580 590 600 VAWRDAQQSS SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI 610 620 630 640 650 660 GNEGSQPIEL TFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFADDILETS ADSLEHVLHF 670 680 690 700 710 720 ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPNPLVS SVFHRKRVFC NFQYAPQLAE 730 740 750 760 770 780 ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP ILRYMWGTDT YRESIKDLAD YASKNLEAMN 790 800 810 820 830 840 PPLFLRFLNL LMNDAIFLLD EAIQYLSKIK IQQIEKDRGE WDSLTPEARR EKEAGLQMFG 850 860 870 880 890 900 QLARFHNIMS NETIGTLAFL TSEIKSLFVH PFLAERIISM LNYFLQHLVG PKMGALKVKD 910 920 930 940 950 960 FSEFDFKPQQ LVSDICTIYL NLGDEENFCA TVPKDGRSYS PTLFAQTVRV LKKINKPGNM 970 980 990 1000 1010 1020 IMAFSNLAER IKSLADLQQQ EEETYADACD EFLDPIMSTL MCDPVVLPSS RVTVDRSTIA 1030 1040 1050 1060 RHLLSDQTDP FNRSPLTMDQ IRPNTELKEK IQRWLAERKQ QKEQLE
	Isoform 2 [UniParc]. Checksum: 11AC6B62DD4076E7	FASTA	1,073	123,522
	10 20 30 40 50 60 MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE 70 80 90 100 110 120 FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD PSLKSGNGIP SRCVYLEEMA 130 140 150 160 170 180 VELEDQDWLD MSNVEQALFA RLLLQDPGNH LINMTSSTTL NLSADRDAGE RHIFCYLYSC 190 200 210 220 230 240 FQRAKEEITK VPENLLPFAV QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA 250 260 270 280 290 300 REYMNKIYFE DVTEFLEEVI EALILDEEVR TFPEVMIPVF DILLGRIKDL ELCQILLYAY 310 320 330 340 350 360 LDILLYFTRQ KDMAKVFVEY IQPKDPTNGQ MYQKTLLGVI LSISCLLKTP GVVENHGYFL 370 380 390 400 410 420 NPSRSSPQEI KVQEANIHQF MAQFHEKIYQ MLKNLLQLSP ETKHCILSWL GNCLHANAGR 430 440 450 460 470 480 TKIWANQMPE IFFQMYASDA FFLNLGAALL KLCQPFCKPR SSRLLTFNPT YCALKELNDE 490 500 510 520 530 540 ERKIKNVHMR GLDKETCLIP AVQEPKFPQN YNLVTENLAL TEYTLYLGFH RLHDQMVKIN 550 560 570 580 590 600 QNLHRLQVAW RDAQQSSSPA ADNLREQFER LMTIYLSTKT AMTEPQMLQN CLNLQVSMAV 610 620 630 640 650 660 LLVQLAIGNE GSQPIELTFP LPDGYSSLAY VPEFFADNLG DFLIFLRRFA DDILETSADS 670 680 690 700 710 720 LEHVLHFITI FTGSIERMKN PHLRAKLAEV LEAVMPHLDQ TPNPLVSSVF HRKRVFCNFQ 730 740 750 760 770 780 YAPQLAEALI KVFVDIEFTG DPHQFEQKFN YRRPMYPILR YMWGTDTYRE SIKDLADYAS 790 800 810 820 830 840 KNLEAMNPPL FLRFLNLLMN DAIFLLDEAI QYLSKIKIQQ IEKDRGEWDS LTPEARREKE 850 860 870 880 890 900 AGLQMFGQLA RFHNIMSNET IGTLAFLTSE IKSLFVHPFL AERIISMLNY FLQHLVGPKM 910 920 930 940 950 960 GALKVKDFSE FDFKPQQLVS DICTIYLNLG DEENFCATVP KDGRSYSPTL FAQTVRVLKK 970 980 990 1000 1010 1020 INKPGNMIMA FSNLAERIKS LADLQQQEEE TYADACDEFL DPIMSTLMCD PVVLPSSRVT 1030 1040 1050 1060 1070 VDRSTIARHL LSDQTDPFNR SPLTMDQIRP NTELKEKIQR WLAERKQQKE QLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed: 8590280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Bone marrow.
[2]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 545-558. Tissue: Platelet.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-386, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Solution structure of the U-box in human ubiquitin conjugation factor E4A." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 977-1061.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50916 mRNA. Translation: BAA09475.2. Different initiation.
EF445042 Genomic DNA. Translation: ACA06094.1.
CH471065 Genomic DNA. Translation: EAW67371.1.
BC052643 mRNA. Translation: AAH52643.1. Sequence problems.
BC062695 mRNA. Translation: AAH62695.1. Sequence problems.
BC111417 mRNA. Translation: AAI11418.1.
BC112367 mRNA. Translation: AAI12368.1.

IPI

IPI00028957.
IPI00556075.

RefSeq

NP_001191006.1. NM_001204077.1.
NP_004779.2. NM_004788.3.

UniGene

Hs.602967.
Hs.75275.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WGM	NMR	-	A	977-1061	[»]

ProteinModelPortal

Q14139.

SMR

Q14139. Positions 82-1061.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14139. 5 interactions.

STRING

Q14139.

PTM databases

PhosphoSite

Q14139.

Polymorphism databases

DMDM

71164791.

Proteomic databases

PRIDE

Q14139.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000431736; ENSP00000387362; ENSG00000110344.

GeneID

9354.

KEGG

hsa:9354.

UCSC

uc001psv.1. human.
uc001psw.1. human.

Organism-specific databases

CTD

9354.

GeneCards

GC11P118264.

H-InvDB

HIX0010181.

HGNC

HGNC:12499. UBE4A.

MIM

603753. gene.

neXtProt

NX_Q14139.

PharmGKB

PA37147.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16160.

GeneTree

ENSGT00390000009300.

HOVERGEN

HBG059871.

InParanoid

Q14139.

OMA

DRDAGEK.

PhylomeDB

Q14139.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

Q14139.

Bgee

Q14139.

CleanEx

HS_UBE4A.

Genevestigator

Q14139.

GermOnline

ENSG00000110344. Homo sapiens.

Family and domain databases

InterPro

IPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

KO

K10596.

Pfam

PF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]

SMART

SM00504. Ubox. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

35029.

SOURCE

Search...

Entry information

Entry name

UBE4A_HUMAN

Accession

Primary (citable) accession number: Q14139
Secondary accession number(s): B0YJB6 , Q2M1H0, Q6P5T4, Q7Z639

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.