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Protein

Ubiquitin conjugation factor E4 A

Gene

UBE4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110344-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 ACurated (EC:6.3.2.-By similarity)
Gene namesi
Name:UBE4AImported
Synonyms:KIAA0126Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:12499. UBE4A.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • ubiquitin ligase complex Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi9354.
OpenTargetsiENSG00000110344.
PharmGKBiPA37147.

Polymorphism and mutation databases

BioMutaiUBE4A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949901 – 1066Ubiquitin conjugation factor E4 AAdd BLAST1066

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ14139.
MaxQBiQ14139.
PaxDbiQ14139.
PeptideAtlasiQ14139.
PRIDEiQ14139.

PTM databases

iPTMnetiQ14139.
PhosphoSitePlusiQ14139.

Miscellaneous databases

PMAP-CutDBB0YJB6.

Expressioni

Gene expression databases

BgeeiENSG00000110344.
CleanExiHS_UBE4A.
ExpressionAtlasiQ14139. baseline and differential.
GenevisibleiQ14139. HS.

Organism-specific databases

HPAiHPA055035.
HPA064010.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MAVSQ7Z4342EBI-1048119,EBI-995373

Protein-protein interaction databases

BioGridi114757. 33 interactors.
IntActiQ14139. 12 interactors.
STRINGi9606.ENSP00000387362.

Structurei

Secondary structure

11066
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni990 – 992Combined sources3
Turni995 – 997Combined sources3
Beta strandi1002 – 1006Combined sources5
Turni1008 – 1010Combined sources3
Beta strandi1013 – 1015Combined sources3
Helixi1016 – 1022Combined sources7
Turni1023 – 1025Combined sources3
Turni1030 – 1032Combined sources3
Turni1038 – 1040Combined sources3
Beta strandi1041 – 1043Combined sources3
Helixi1045 – 1057Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGMNMR-A977-1061[»]
ProteinModelPortaliQ14139.
SMRiQ14139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14139.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini987 – 1061U-boxAdd BLAST75

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.By similarity

Sequence similaritiesi

Contains 1 U-box domain.Curated

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
HOGENOMiHOG000007049.
HOVERGENiHBG059871.
InParanoidiQ14139.
KOiK10596.
OMAiHFEDVTE.
OrthoDBiEOG091G07J9.
PhylomeDBiQ14139.
TreeFamiTF300802.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS
60 70 80 90 100
DNSVSESLDE FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD
110 120 130 140 150
PSLKSGNGIP SRCVYLEEMA VELEDQDWLD MSNVEQALFA RLLLQDPGNH
160 170 180 190 200
LINMTSSTTL NLSADRDAGE RHIFCYLYSC FQRAKEEITK VPENLLPFAV
210 220 230 240 250
QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA HFEDVTEFLE
260 270 280 290 300
EVIEALILDE EVRTFPEVMI PVFDILLGRI KDLELCQILL YAYLDILLYF
310 320 330 340 350
TRQKDMAKVF VEYIQPKDPT NGQMYQKTLL GVILSISCLL KTPGVVENHG
360 370 380 390 400
YFLNPSRSSP QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL
410 420 430 440 450
SWLGNCLHAN AGRTKIWANQ MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC
460 470 480 490 500
KPRSSRLLTF NPTYCALKEL NDEERKIKNV HMRGLDKETC LIPAVQEPKF
510 520 530 540 550
PQNYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ VAWRDAQQSS
560 570 580 590 600
SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI
610 620 630 640 650
GNEGSQPIEL TFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFADDILETS
660 670 680 690 700
ADSLEHVLHF ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPNPLVS
710 720 730 740 750
SVFHRKRVFC NFQYAPQLAE ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP
760 770 780 790 800
ILRYMWGTDT YRESIKDLAD YASKNLEAMN PPLFLRFLNL LMNDAIFLLD
810 820 830 840 850
EAIQYLSKIK IQQIEKDRGE WDSLTPEARR EKEAGLQMFG QLARFHNIMS
860 870 880 890 900
NETIGTLAFL TSEIKSLFVH PFLAERIISM LNYFLQHLVG PKMGALKVKD
910 920 930 940 950
FSEFDFKPQQ LVSDICTIYL NLGDEENFCA TVPKDGRSYS PTLFAQTVRV
960 970 980 990 1000
LKKINKPGNM IMAFSNLAER IKSLADLQQQ EEETYADACD EFLDPIMSTL
1010 1020 1030 1040 1050
MCDPVVLPSS RVTVDRSTIA RHLLSDQTDP FNRSPLTMDQ IRPNTELKEK
1060
IQRWLAERKQ QKEQLE
Length:1,066
Mass (Da):122,561
Last modified:May 10, 2005 - v2
Checksum:iA735D670220F9DDA
GO
Isoform 2 (identifier: Q14139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-241: H → REYMNKIY

Note: No experimental confirmation available.
Show »
Length:1,073
Mass (Da):123,522
Checksum:i11AC6B62DD4076E7
GO

Sequence cautioni

The sequence AAH52643 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH62695 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA09475 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217P → L in BAA09475 (PubMed:8590280).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013673241H → REYMNKIY in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50916 mRNA. Translation: BAA09475.2. Different initiation.
EF445042 Genomic DNA. Translation: ACA06094.1.
CH471065 Genomic DNA. Translation: EAW67371.1.
BC052643 mRNA. Translation: AAH52643.1. Sequence problems.
BC062695 mRNA. Translation: AAH62695.1. Sequence problems.
BC111417 mRNA. Translation: AAI11418.1.
BC112367 mRNA. Translation: AAI12368.1.
CCDSiCCDS55790.1. [Q14139-1]
CCDS8396.1. [Q14139-2]
RefSeqiNP_001191006.1. NM_001204077.1. [Q14139-1]
NP_004779.2. NM_004788.3. [Q14139-2]
UniGeneiHs.602967.
Hs.75275.

Genome annotation databases

EnsembliENST00000252108; ENSP00000252108; ENSG00000110344. [Q14139-1]
ENST00000431736; ENSP00000387362; ENSG00000110344. [Q14139-2]
GeneIDi9354.
KEGGihsa:9354.
UCSCiuc001psv.4. human. [Q14139-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50916 mRNA. Translation: BAA09475.2. Different initiation.
EF445042 Genomic DNA. Translation: ACA06094.1.
CH471065 Genomic DNA. Translation: EAW67371.1.
BC052643 mRNA. Translation: AAH52643.1. Sequence problems.
BC062695 mRNA. Translation: AAH62695.1. Sequence problems.
BC111417 mRNA. Translation: AAI11418.1.
BC112367 mRNA. Translation: AAI12368.1.
CCDSiCCDS55790.1. [Q14139-1]
CCDS8396.1. [Q14139-2]
RefSeqiNP_001191006.1. NM_001204077.1. [Q14139-1]
NP_004779.2. NM_004788.3. [Q14139-2]
UniGeneiHs.602967.
Hs.75275.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGMNMR-A977-1061[»]
ProteinModelPortaliQ14139.
SMRiQ14139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114757. 33 interactors.
IntActiQ14139. 12 interactors.
STRINGi9606.ENSP00000387362.

PTM databases

iPTMnetiQ14139.
PhosphoSitePlusiQ14139.

Polymorphism and mutation databases

BioMutaiUBE4A.

Proteomic databases

EPDiQ14139.
MaxQBiQ14139.
PaxDbiQ14139.
PeptideAtlasiQ14139.
PRIDEiQ14139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252108; ENSP00000252108; ENSG00000110344. [Q14139-1]
ENST00000431736; ENSP00000387362; ENSG00000110344. [Q14139-2]
GeneIDi9354.
KEGGihsa:9354.
UCSCiuc001psv.4. human. [Q14139-1]

Organism-specific databases

CTDi9354.
DisGeNETi9354.
GeneCardsiUBE4A.
HGNCiHGNC:12499. UBE4A.
HPAiHPA055035.
HPA064010.
MIMi603753. gene.
neXtProtiNX_Q14139.
OpenTargetsiENSG00000110344.
PharmGKBiPA37147.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
HOGENOMiHOG000007049.
HOVERGENiHBG059871.
InParanoidiQ14139.
KOiK10596.
OMAiHFEDVTE.
OrthoDBiEOG091G07J9.
PhylomeDBiQ14139.
TreeFamiTF300802.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000110344-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiUBE4A. human.
EvolutionaryTraceiQ14139.
GeneWikiiUBE4A.
GenomeRNAii9354.
PMAP-CutDBB0YJB6.
PROiQ14139.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110344.
CleanExiHS_UBE4A.
ExpressionAtlasiQ14139. baseline and differential.
GenevisibleiQ14139. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE4A_HUMAN
AccessioniPrimary (citable) accession number: Q14139
Secondary accession number(s): B0YJB6
, Q2M1H0, Q6P5T4, Q7Z639
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.