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Q14137

- BOP1_HUMAN

UniProt

Q14137 - BOP1_HUMAN

Protein

Ribosome biogenesis protein BOP1

Gene

BOP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 2 (10 Oct 2002)
      Previous versions | rss
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    Functioni

    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.1 PublicationUniRule annotation

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. ribonucleoprotein complex binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
    3. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
    4. regulation of cell cycle Source: UniProtKB
    5. ribosome biogenesis Source: UniProtKB

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    SignaLinkiQ14137.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome biogenesis protein BOP1UniRule annotation
    Alternative name(s):
    Block of proliferation 1 proteinUniRule annotation
    Gene namesi
    Name:BOP1UniRule annotation
    Synonyms:KIAA0124
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:15519. BOP1.

    Subcellular locationi

    Nucleusnucleolus 1 PublicationUniRule annotation. Nucleusnucleoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB-SubCell
    2. PeBoW complex Source: UniProtKB
    3. preribosome, large subunit precursor Source: UniProtKB-HAMAP
    4. ribonucleoprotein complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25398.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 746746Ribosome biogenesis protein BOP1PRO_0000050885Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei106 – 1061Phosphothreonine2 Publications
    Modified residuei126 – 1261PhosphoserineBy similarity
    Modified residuei127 – 1271PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ14137.
    PaxDbiQ14137.
    PeptideAtlasiQ14137.
    PRIDEiQ14137.

    2D gel databases

    SWISS-2DPAGEQ14137.

    PTM databases

    PhosphoSiteiQ14137.

    Expressioni

    Inductioni

    By MYC.1 Publication

    Gene expression databases

    BgeeiQ14137.
    CleanExiHS_BOP1.
    GenevestigatoriQ14137.

    Organism-specific databases

    HPAiHPA047869.
    HPA048336.

    Interactioni

    Subunit structurei

    Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome.4 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PES1O005413EBI-1050828,EBI-1053271
    WDR12Q9GZL73EBI-1050828,EBI-2490660

    Protein-protein interaction databases

    BioGridi116850. 33 interactions.
    IntActiQ14137. 10 interactions.
    MINTiMINT-3029124.
    STRINGi9606.ENSP00000304151.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14137.
    SMRiQ14137. Positions 410-745.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati411 – 45040WD 1Add
    BLAST
    Repeati452 – 49241WD 2Add
    BLAST
    Repeati532 – 57645WD 3Add
    BLAST
    Repeati577 – 61539WD 4Add
    BLAST
    Repeati618 – 65740WD 5Add
    BLAST
    Repeati661 – 70040WD 6Add
    BLAST
    Repeati716 – 74631WD 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 427163Sufficient for nucleolar localizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat BOP1/ERB1 family.UniRule annotation
    Contains 7 WD repeats.UniRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000029368.
    HOVERGENiHBG008113.
    InParanoidiQ14137.
    KOiK14824.
    OMAiWYEDFPH.
    PhylomeDBiQ14137.
    TreeFamiTF300437.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    HAMAPiMF_03027. BOP1.
    InterProiIPR028598. BOP1/Erb1.
    IPR012953. BOP1_N_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08145. BOP1NT. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view]
    SMARTiSM01035. BOP1NT. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14137-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS    50
    GVSDSEESVF SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE 100
    QVQASTPCPR TEMASARIGD EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV 150
    GYDLDGRRIY KPLRTRDELD QFLDKMDDPD YWRTVQDPMT GRDLRLTDEQ 200
    VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP ADKRSFIPSL 250
    VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV 300
    PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL 350
    RAVPAYGRFI QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP 400
    FPTCQALVYR GHSDLVRCLS VSPGGQWLVS GSDDGSLRLW EVATARCVRT 450
    VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL LLNPALGDRL VAGSTDQLLS 500
    AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV TWHGRGDYLA 550
    VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR 600
    SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD 650
    LSTKPYRMLR HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ 700
    NPLLVPVKVL KGHVLTRDLG VLDVIFHPTQ PWVFSSGADG TVRLFT 746
    Length:746
    Mass (Da):83,630
    Last modified:October 10, 2002 - v2
    Checksum:i49A97BE21B0EB3DD
    GO
    Isoform 2 (identifier: Q14137-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-112: Missing.

    Show »
    Length:634
    Mass (Da):71,930
    Checksum:iE7A5608B28D65C36
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti577 – 5771R → H in BAA09473. (PubMed:8590280)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 112112Missing in isoform 2. 1 PublicationVSP_056391Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB060694 mRNA. Translation: BAB70666.1.
    AC145291 Genomic DNA. No translation available.
    AC231662 Genomic DNA. No translation available.
    BC001086 mRNA. Translation: AAH01086.2.
    BC005160 mRNA. Translation: AAH05160.2.
    BC007274 mRNA. Translation: AAH07274.1.
    BC013787 mRNA. Translation: AAH13787.1.
    BC013980 mRNA. Translation: AAH13980.1.
    BC017674 mRNA. Translation: AAH17674.1.
    D50914 mRNA. Translation: BAA09473.1.
    CCDSiCCDS6418.1.
    RefSeqiNP_056016.1. NM_015201.3.
    UniGeneiHs.645279.

    Genome annotation databases

    EnsembliENST00000377412; ENSP00000366629; ENSG00000204775.
    ENST00000569669; ENSP00000455106; ENSG00000261236.
    GeneIDi23246.
    KEGGihsa:23246.
    UCSCiuc003zbm.3. human.

    Polymorphism databases

    DMDMi23830903.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB060694 mRNA. Translation: BAB70666.1 .
    AC145291 Genomic DNA. No translation available.
    AC231662 Genomic DNA. No translation available.
    BC001086 mRNA. Translation: AAH01086.2 .
    BC005160 mRNA. Translation: AAH05160.2 .
    BC007274 mRNA. Translation: AAH07274.1 .
    BC013787 mRNA. Translation: AAH13787.1 .
    BC013980 mRNA. Translation: AAH13980.1 .
    BC017674 mRNA. Translation: AAH17674.1 .
    D50914 mRNA. Translation: BAA09473.1 .
    CCDSi CCDS6418.1.
    RefSeqi NP_056016.1. NM_015201.3.
    UniGenei Hs.645279.

    3D structure databases

    ProteinModelPortali Q14137.
    SMRi Q14137. Positions 410-745.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116850. 33 interactions.
    IntActi Q14137. 10 interactions.
    MINTi MINT-3029124.
    STRINGi 9606.ENSP00000304151.

    PTM databases

    PhosphoSitei Q14137.

    Polymorphism databases

    DMDMi 23830903.

    2D gel databases

    SWISS-2DPAGE Q14137.

    Proteomic databases

    MaxQBi Q14137.
    PaxDbi Q14137.
    PeptideAtlasi Q14137.
    PRIDEi Q14137.

    Protocols and materials databases

    DNASUi 23246.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000377412 ; ENSP00000366629 ; ENSG00000204775 .
    ENST00000569669 ; ENSP00000455106 ; ENSG00000261236 .
    GeneIDi 23246.
    KEGGi hsa:23246.
    UCSCi uc003zbm.3. human.

    Organism-specific databases

    CTDi 23246.
    GeneCardsi GC08M145486.
    H-InvDB HIX0168814.
    HGNCi HGNC:15519. BOP1.
    HPAi HPA047869.
    HPA048336.
    MIMi 610596. gene.
    neXtProti NX_Q14137.
    PharmGKBi PA25398.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000029368.
    HOVERGENi HBG008113.
    InParanoidi Q14137.
    KOi K14824.
    OMAi WYEDFPH.
    PhylomeDBi Q14137.
    TreeFami TF300437.

    Enzyme and pathway databases

    SignaLinki Q14137.

    Miscellaneous databases

    GeneWikii BOP1.
    GenomeRNAii 23246.
    NextBioi 44934.
    PROi Q14137.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q14137.
    CleanExi HS_BOP1.
    Genevestigatori Q14137.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    HAMAPi MF_03027. BOP1.
    InterProi IPR028598. BOP1/Erb1.
    IPR012953. BOP1_N_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08145. BOP1NT. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view ]
    SMARTi SM01035. BOP1NT. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning of immunogenic antigens overexpressed in pancreatic cancer."
      Nakatsura T., Senju S., Yamada K., Jotsuka T., Ogawa M., Nishimura Y.
      Biochem. Biophys. Res. Commun. 281:936-944(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain, Eye, Lymph, Muscle and Pancreas.
    4. "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."
      Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N.
      DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-746 (ISOFORM 1).
      Tissue: Bone marrow.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation."
      Hoelzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm G.W., Eick D.
      J. Cell Biol. 170:367-378(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PES1 AND WDR12, INDUCTION.
    7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex."
      Grimm T., Hoelzel M., Rohrmoser M., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Eick D.
      Nucleic Acids Res. 34:3030-3043(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PES1 AND WDR12.
    9. "Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit."
      Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D.
      Mol. Cell. Biol. 27:3682-3694(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PES1 AND WDR12.
    10. "The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing."
      Hoelzel M., Grimm T., Rohrmoser M., Malamoussi A., Harasim T., Gruber-Eber A., Kremmer E., Eick D.
      Nucleic Acids Res. 35:789-800(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PES1 AND WDR12.
    11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiBOP1_HUMAN
    AccessioniPrimary (citable) accession number: Q14137
    Secondary accession number(s): Q969Z6
    , Q96IS8, Q96Q25, Q9BSA7, Q9BVM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 10, 2002
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3