Q14137 (BOP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome biogenesis protein BOP1

Alternative name(s):
Block of proliferation 1 protein

Gene names

Name:	BOP1
Synonyms:	KIAA0124

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.7
Subunit structure	Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Ref.4 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm Ref.3.
Induction	By MYC. Ref.4
Sequence similarities	Belongs to the WD repeat BOP1/ERB1 family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Non-traceable author statement Ref.4. Source: UniProtKB maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.7. Source: UniProtKB regulation of cell cycle Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	PeBoW complex Inferred from direct assay Ref.4Ref.7. Source: UniProtKB nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PES1	O00541	3	EBI-1050828,EBI-1053271
WDR12	Q9GZL7	3	EBI-1050828,EBI-2490660

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 746	746	Ribosome biogenesis protein BOP1		PRO_0000050885
Regions
	Repeat	411 – 450	40	WD 1
	Repeat	452 – 492	41	WD 2
	Repeat	532 – 576	45	WD 3
	Repeat	577 – 615	39	WD 4
	Repeat	618 – 657	40	WD 5
	Repeat	661 – 700	40	WD 6
	Repeat	716 – 746	31	WD 7
	Region	265 – 427	163	Sufficient for nucleolar localization By similarity
Amino acid modifications
	Modified residue	106	1	Phosphothreonine Ref.5 Ref.9
	Modified residue	126	1	Phosphoserine By similarity
	Modified residue	127	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	577	1	R → H in BAA09473. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q14137 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: 49A97BE21B0EB3DD	FASTA	746	83,630
	10 20 30 40 50 60 MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF 70 80 90 100 110 120 SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE QVQASTPCPR TEMASARIGD 130 140 150 160 170 180 EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV GYDLDGRRIY KPLRTRDELD QFLDKMDDPD 190 200 210 220 230 240 YWRTVQDPMT GRDLRLTDEQ VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP 250 260 270 280 290 300 ADKRSFIPSL VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV 310 320 330 340 350 360 PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL RAVPAYGRFI 370 380 390 400 410 420 QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP FPTCQALVYR GHSDLVRCLS 430 440 450 460 470 480 VSPGGQWLVS GSDDGSLRLW EVATARCVRT VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL 490 500 510 520 530 540 LLNPALGDRL VAGSTDQLLS AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV 550 560 570 580 590 600 TWHGRGDYLA VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR 610 620 630 640 650 660 SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD LSTKPYRMLR 670 680 690 700 710 720 HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ NPLLVPVKVL KGHVLTRDLG 730 740 VLDVIFHPTQ PWVFSSGADG TVRLFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Eye, Lymph, Muscle and Pancreas.
[2]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-746. Tissue: Bone marrow.
[3]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation." Hoelzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm G.W., Eick D. J. Cell Biol. 170:367-378(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12, INDUCTION.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex." Grimm T., Hoelzel M., Rohrmoser M., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Eick D. Nucleic Acids Res. 34:3030-3043(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12.
[7]	"Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit." Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D. Mol. Cell. Biol. 27:3682-3694(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PES1 AND WDR12.
[8]	"The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing." Hoelzel M., Grimm T., Rohrmoser M., Malamoussi A., Harasim T., Gruber-Eber A., Kremmer E., Eick D. Nucleic Acids Res. 35:789-800(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC001086 mRNA. Translation: AAH01086.2. BC005160 mRNA. Translation: AAH05160.2. BC007274 mRNA. Translation: AAH07274.1. BC013787 mRNA. Translation: AAH13787.1. BC013980 mRNA. Translation: AAH13980.1. BC017674 mRNA. Translation: AAH17674.1. D50914 mRNA. Translation: BAA09473.1.
IPI	IPI00028955.
RefSeq	NP_056016.1. NM_015201.3.
UniGene	Hs.645279.
3D structure databases
ProteinModelPortal	Q14137.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14137. 3 interactions.
STRING	9606.ENSP00000304151.
PTM databases
PhosphoSite	Q14137.
Polymorphism databases
DMDM	23830903.
2D gel databases
SWISS-2DPAGE	Q14137.
Proteomic databases
PaxDb	Q14137.
PeptideAtlas	Q14137.
PRIDE	Q14137.
Protocols and materials databases
DNASU	23246.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307404; ENSP00000304151; ENSG00000170727. ENST00000569669; ENSP00000455106; ENSG00000261236.
GeneID	23246.
KEGG	hsa:23246.
UCSC	uc003zbm.3. human.
Organism-specific databases
CTD	23246.
GeneCards	GC08M145456.
H-InvDB	HIX0168814.
HGNC	HGNC:15519. BOP1.
MIM	610596. gene.
neXtProt	NX_Q14137.
PharmGKB	PA25398.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000029368.
HOVERGEN	HBG008113.
InParanoid	Q14137.
KO	K14824.
OMA	PPPEYLF.
OrthoDB	EOG44XJGB.
PhylomeDB	Q14137.
Gene expression databases
ArrayExpress	Q14137.
Bgee	Q14137.
CleanEx	HS_BOP1.
Genevestigator	Q14137.
GermOnline	ENSG00000170727. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR012953. BOP1_N_dom. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF08145. BOP1NT. 1 hit. PF00400. WD40. 3 hits. [Graphical view]
SMART	SM01035. BOP1NT. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23246.
NextBio	44934.
SOURCE	Search...

Entry information

Entry name

BOP1_HUMAN

Accession

Primary (citable) accession number: Q14137
Secondary accession number(s): Q969Z6 , Q96IS8, Q9BSA7, Q9BVM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2002
Last modified:	May 1, 2013
This is version 139 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.