Reviewed, UniProtKB/Swiss-Prot Q14137 (BOP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Ribosome biogenesis protein BOP1
Alternative name(s):
    Block of proliferation 1 protein

Gene names

Name:	BOP1
Synonyms:	KIAA0124

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.9
Subunit structure	Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Ref.9 Ref.4 Ref.7 Ref.10
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Ref.3
Induction	By MYC. Ref.4
Sequence similarities	Belongs to the WD repeat BOP1/ERB1 family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell proliferation Ref.4 Non-traceable author statement. Source: UniProtKB maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.9 Inferred from mutant phenotype. Source: UniProtKB regulation of cell cycle Ref.4 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	PeBoW complex Ref.4 Ref.9 Inferred from direct assay. Source: UniProtKB nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.4 Ref.7 Ref.9 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARRB1	P49407	1	EBI-1050828,EBI-743313
ARRB2	P32121	1	EBI-1050828,EBI-714559

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 746	746	Ribosome biogenesis protein BOP1		PRO_0000050885
Regions
	Repeat	411 – 450	40	WD 1
	Repeat	452 – 492	41	WD 2
	Repeat	532 – 576	45	WD 3
	Repeat	577 – 615	39	WD 4
	Repeat	618 – 657	40	WD 5
	Repeat	661 – 700	40	WD 6
	Repeat	716 – 746	31	WD 7
	Region	265 – 427	163	Sufficient for nucleolar localization By similarity
Amino acid modifications
	Modified residue	106	1	Phosphothreonine Ref.6 Ref.11 Ref.12 Ref.14
	Modified residue	126	1	Phosphoserine Ref.14 Ref.5 Ref.8 Ref.13 Ref.15
	Modified residue	127	1	Phosphoserine Ref.14 Ref.5 Ref.8 Ref.13 Ref.15
	Modified residue	197	1	Phosphothreonine Ref.14
Experimental info
	Sequence conflict	577	1	R → H in BAA09473. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q14137-1 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: 49A97BE21B0EB3DD	FASTA	746	83,630
	10 20 30 40 50 60 MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF 70 80 90 100 110 120 SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE QVQASTPCPR TEMASARIGD 130 140 150 160 170 180 EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV GYDLDGRRIY KPLRTRDELD QFLDKMDDPD 190 200 210 220 230 240 YWRTVQDPMT GRDLRLTDEQ VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP 250 260 270 280 290 300 ADKRSFIPSL VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV 310 320 330 340 350 360 PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL RAVPAYGRFI 370 380 390 400 410 420 QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP FPTCQALVYR GHSDLVRCLS 430 440 450 460 470 480 VSPGGQWLVS GSDDGSLRLW EVATARCVRT VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL 490 500 510 520 530 540 LLNPALGDRL VAGSTDQLLS AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV 550 560 570 580 590 600 TWHGRGDYLA VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR 610 620 630 640 650 660 SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD LSTKPYRMLR 670 680 690 700 710 720 HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ NPLLVPVKVL KGHVLTRDLG 730 740 VLDVIFHPTQ PWVFSSGADG TVRLFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Eye, Lymph, Muscle and Pancreas.
[2]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed: 8590280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-746. Tissue: Bone marrow.
[3]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation." Hoelzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm G.W., Eick D. J. Cell Biol. 170:367-378(2005) [PubMed: 16043514] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12, INDUCTION.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-127, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex." Grimm T., Hoelzel M., Rohrmoser M., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Eick D. Nucleic Acids Res. 34:3030-3043(2006) [PubMed: 16738141] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12.
[8]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-127, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit." Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D. Mol. Cell. Biol. 27:3682-3694(2007) [PubMed: 17353269] [Abstract] Cited for: FUNCTION, INTERACTION WITH PES1 AND WDR12.
[10]	"The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing." Hoelzel M., Grimm T., Rohrmoser M., Malamoussi A., Harasim T., Gruber-Eber A., Kremmer E., Eick D. Nucleic Acids Res. 35:789-800(2007) [PubMed: 17189298] [Abstract] Cited for: INTERACTION WITH PES1 AND WDR12.
[11]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, MASS SPECTROMETRY.
[12]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, MASS SPECTROMETRY.
[13]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-127, MASS SPECTROMETRY.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106; SER-126; SER-127 AND THR-197, MASS SPECTROMETRY.
[15]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-127, MASS SPECTROMETRY. Tissue: Liver.
[16]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC001086 mRNA. Translation: AAH01086.2. BC005160 mRNA. Translation: AAH05160.2. BC007274 mRNA. Translation: AAH07274.1. BC013787 mRNA. Translation: AAH13787.1. BC013980 mRNA. Translation: AAH13980.1. BC017674 mRNA. Translation: AAH17674.1. D50914 mRNA. Translation: BAA09473.1.
IPI	IPI00028955.
RefSeq	NP_056016.1.
UniGene	Hs.535901 Hs.645279
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14137. 2 interactions.
STRING	Q14137.
PTM databases
PhosphoSite	Q14137.
2-D gel databases
SWISS-2DPAGE	Q14137.
Proteomic databases
PeptideAtlas	Q14137.
PRIDE	Q14137.
Genome annotation databases
Ensembl	ENST00000307404; ENSP00000304151; ENSG00000170727; Homo sapiens. [Genome view]
GeneID	23246.
KEGG	hsa:23246.
UCSC	uc003zbr.1. human.
Organism-specific databases
CTD	23246.
GeneCards	GC08M145456.
H-InvDB	HIX0007848. HIX0034600.
HGNC	HGNC:15519. BOP1.
MIM	610596. gene.
PharmGKB	PA25398.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q14137.
HOVERGEN	Q14137.
OMA	MASARIG.
Gene expression databases
CleanEx	HS_BOP1.
Genevestigator	Q14137.
GermOnline	ENSG00000170727. Homo sapiens.
Family and domain databases
InterPro	IPR012953. BOP1_N. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF08145. BOP1NT. 1 hit. PF00400. WD40. 4 hits. [Graphical view]
ProDom	PD000018. WD40. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44934.
SOURCE	Search...

Entry information

Entry name

BOP1_HUMAN

Accession

Primary (citable) accession number: Q14137
Secondary accession number(s): Q969Z6 , Q96IS8, Q9BSA7, Q9BVM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2002
Last modified:	November 3, 2009
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.