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Q14137 (BOP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome biogenesis protein BOP1
Alternative name(s):
Block of proliferation 1 protein
Gene names
Name:BOP1
Synonyms:KIAA0124
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.7

Subunit structure

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Ref.4 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm Ref.3.

Induction

By MYC. Ref.4

Sequence similarities

Belongs to the WD repeat BOP1/ERB1 family.

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 746746Ribosome biogenesis protein BOP1 HAMAP-Rule MF_03027
PRO_0000050885

Regions

Repeat411 – 45040WD 1 HAMAP-Rule MF_03027
Repeat452 – 49241WD 2 HAMAP-Rule MF_03027
Repeat532 – 57645WD 3 HAMAP-Rule MF_03027
Repeat577 – 61539WD 4 HAMAP-Rule MF_03027
Repeat618 – 65740WD 5 HAMAP-Rule MF_03027
Repeat661 – 70040WD 6 HAMAP-Rule MF_03027
Repeat716 – 74631WD 7 HAMAP-Rule MF_03027
Region265 – 427163Sufficient for nucleolar localization By similarity

Amino acid modifications

Modified residue1061Phosphothreonine Ref.5 Ref.9
Modified residue1261Phosphoserine By similarity
Modified residue1271Phosphoserine By similarity

Experimental info

Sequence conflict5771R → H in BAA09473. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q14137 [UniParc].

Last modified October 10, 2002. Version 2.
Checksum: 49A97BE21B0EB3DD

FASTA74683,630
        10         20         30         40         50         60 
MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF 

        70         80         90        100        110        120 
SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE QVQASTPCPR TEMASARIGD 

       130        140        150        160        170        180 
EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV GYDLDGRRIY KPLRTRDELD QFLDKMDDPD 

       190        200        210        220        230        240 
YWRTVQDPMT GRDLRLTDEQ VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP 

       250        260        270        280        290        300 
ADKRSFIPSL VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV 

       310        320        330        340        350        360 
PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL RAVPAYGRFI 

       370        380        390        400        410        420 
QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP FPTCQALVYR GHSDLVRCLS 

       430        440        450        460        470        480 
VSPGGQWLVS GSDDGSLRLW EVATARCVRT VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL 

       490        500        510        520        530        540 
LLNPALGDRL VAGSTDQLLS AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV 

       550        560        570        580        590        600 
TWHGRGDYLA VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR 

       610        620        630        640        650        660 
SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD LSTKPYRMLR 

       670        680        690        700        710        720 
HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ NPLLVPVKVL KGHVLTRDLG 

       730        740 
VLDVIFHPTQ PWVFSSGADG TVRLFT 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Eye, Lymph, Muscle and Pancreas.
[2]"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."
Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N.
DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-746.
Tissue: Bone marrow.
[3]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[4]"Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation."
Hoelzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm G.W., Eick D.
J. Cell Biol. 170:367-378(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PES1 AND WDR12, INDUCTION.
[5]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex."
Grimm T., Hoelzel M., Rohrmoser M., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Eick D.
Nucleic Acids Res. 34:3030-3043(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PES1 AND WDR12.
[7]"Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit."
Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D.
Mol. Cell. Biol. 27:3682-3694(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PES1 AND WDR12.
[8]"The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing."
Hoelzel M., Grimm T., Rohrmoser M., Malamoussi A., Harasim T., Gruber-Eber A., Kremmer E., Eick D.
Nucleic Acids Res. 35:789-800(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PES1 AND WDR12.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC001086 mRNA. Translation: AAH01086.2.
BC005160 mRNA. Translation: AAH05160.2.
BC007274 mRNA. Translation: AAH07274.1.
BC013787 mRNA. Translation: AAH13787.1.
BC013980 mRNA. Translation: AAH13980.1.
BC017674 mRNA. Translation: AAH17674.1.
D50914 mRNA. Translation: BAA09473.1.
RefSeqNP_056016.1. NM_015201.3.
UniGeneHs.645279.

3D structure databases

ProteinModelPortalQ14137.
SMRQ14137. Positions 410-745.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116850. 31 interactions.
IntActQ14137. 8 interactions.
MINTMINT-3029124.
STRING9606.ENSP00000304151.

PTM databases

PhosphoSiteQ14137.

Polymorphism databases

DMDM23830903.

2D gel databases

SWISS-2DPAGEQ14137.

Proteomic databases

PaxDbQ14137.
PeptideAtlasQ14137.
PRIDEQ14137.

Protocols and materials databases

DNASU23246.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307404; ENSP00000304151; ENSG00000170727.
ENST00000569669; ENSP00000455106; ENSG00000261236.
GeneID23246.
KEGGhsa:23246.
UCSCuc003zbm.3. human.

Organism-specific databases

CTD23246.
GeneCardsGC08M145486.
H-InvDBHIX0168814.
HGNCHGNC:15519. BOP1.
HPAHPA047869.
HPA048336.
MIM610596. gene.
neXtProtNX_Q14137.
PharmGKBPA25398.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000029368.
HOVERGENHBG008113.
InParanoidQ14137.
KOK14824.
OMAQIFHGKV.
PhylomeDBQ14137.
TreeFamTF300437.

Enzyme and pathway databases

SignaLinkQ14137.

Gene expression databases

BgeeQ14137.
CleanExHS_BOP1.
GenevestigatorQ14137.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
HAMAPMF_03027. BOP1.
InterProIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF08145. BOP1NT. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTSM01035. BOP1NT. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiBOP1.
GenomeRNAi23246.
NextBio44934.
PROQ14137.
SOURCESearch...

Entry information

Entry nameBOP1_HUMAN
AccessionPrimary (citable) accession number: Q14137
Secondary accession number(s): Q969Z6 expand/collapse secondary AC list , Q96IS8, Q9BSA7, Q9BVM0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2002
Last modified: April 16, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM