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Protein

Cyclic nucleotide-gated cation channel beta-1

Gene

CNGB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP.
Isoform GARP2 is a high affinity rod photoreceptor phosphodiesterase (PDE6)-binding protein that modulates its catalytic properties: it is a regulator of spontaneous activation of rod PDE6, thereby serving to lower rod photoreceptor 'dark noise' and allowing these sensory cells to operate at the single photon detection limit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1030cAMPSequence analysis1
Binding sitei1042cAMPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi970 – 1109cAMPBy similarityAdd BLAST140

GO - Molecular functioni

  • cAMP binding Source: UniProtKB-KW
  • cGMP binding Source: UniProtKB
  • intracellular cAMP activated cation channel activity Source: GO_Central
  • intracellular cGMP activated cation channel activity Source: UniProtKB
  • ligand-gated ion channel activity Source: ProtInc
  • voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

  • cation transport Source: UniProtKB
  • detection of light stimulus involved in visual perception Source: UniProtKB
  • photoreceptor cell maintenance Source: Ensembl
  • photoreceptor cell outer segment organization Source: Ensembl
  • protein heterotetramerization Source: Ensembl
  • protein localization to organelle Source: Ensembl
  • regulation of cytosolic calcium ion concentration Source: Ensembl
  • regulation of membrane potential Source: GO_Central
  • regulation of rhodopsin mediated signaling pathway Source: Reactome
  • retina homeostasis Source: UniProtKB
  • rhodopsin mediated signaling pathway Source: Reactome
  • sensory perception of smell Source: UniProtKB-KW
  • transport Source: ProtInc
  • visual perception Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Olfaction, Sensory transduction, Transport, Vision

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000070729-MONOMER.
ReactomeiR-HSA-2485179. Activation of the phototransduction cascade.
R-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-HSA-5620916. VxPx cargo-targeting to cilium.

Protein family/group databases

TCDBi1.A.1.5.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated cation channel beta-1
Alternative name(s):
Cyclic nucleotide-gated cation channel 4
Short name:
CNG channel 4
Short name:
CNG-4
Short name:
CNG4
Cyclic nucleotide-gated cation channel gamma
Cyclic nucleotide-gated cation channel modulatory subunit
Cyclic nucleotide-gated channel beta-1
Short name:
CNG channel beta-1
Glutamic acid-rich protein
Short name:
GARP
Gene namesi
Name:CNGB1
Synonyms:CNCG2, CNCG3L, CNCG4, RCNC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:2151. CNGB1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini343 – 656CytoplasmicSequence analysisAdd BLAST314
Transmembranei657 – 675Helical; Name=H1Sequence analysisAdd BLAST19
Topological domaini676 – 689ExtracellularSequence analysisAdd BLAST14
Transmembranei690 – 708Helical; Name=H2Sequence analysisAdd BLAST19
Topological domaini709 – 733CytoplasmicSequence analysisAdd BLAST25
Transmembranei734 – 753Helical; Name=H3Sequence analysisAdd BLAST20
Topological domaini754 – 790ExtracellularSequence analysisAdd BLAST37
Transmembranei791 – 813Helical; Name=H4Sequence analysisAdd BLAST23
Topological domaini814 – 857CytoplasmicSequence analysisAdd BLAST44
Transmembranei858 – 877Helical; Name=H5Sequence analysisAdd BLAST20
Topological domaini878 – 961ExtracellularSequence analysisAdd BLAST84
Transmembranei962 – 982Helical; Name=H6Sequence analysisAdd BLAST21
Topological domaini983 – 1251CytoplasmicSequence analysisAdd BLAST269

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Retinitis pigmentosa 45 (RP45)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
See also OMIM:613767
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060491993G → V in RP45. 1 PublicationCorresponds to variant rs121918532dbSNPEnsembl.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi568L → E: Loss of calcium/calmodulin modulation. 1 Publication1

Keywords - Diseasei

Disease mutation, Retinitis pigmentosa

Organism-specific databases

DisGeNETi1258.
MalaCardsiCNGB1.
MIMi613767. phenotype.
OpenTargetsiENSG00000070729.
Orphaneti791. Retinitis pigmentosa.
PharmGKBiPA26662.

Polymorphism and mutation databases

BioMutaiCNGB1.
DMDMi257051004.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193231 – 1251Cyclic nucleotide-gated cation channel beta-1Add BLAST1251

Proteomic databases

EPDiQ14028.
PaxDbiQ14028.
PeptideAtlasiQ14028.
PRIDEiQ14028.

PTM databases

iPTMnetiQ14028.
PhosphoSitePlusiQ14028.

Expressioni

Gene expression databases

BgeeiENSG00000070729.
CleanExiHS_CNGB1.
ExpressionAtlasiQ14028. baseline and differential.
GenevisibleiQ14028. HS.

Organism-specific databases

HPAiHPA039159.
HPA060355.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GRB14Q5ICW42EBI-7959609,EBI-7639273From a different organism.

Protein-protein interaction databases

BioGridi107658. 3 interactors.
IntActiQ14028. 2 interactors.
MINTiMINT-4853474.
STRINGi9606.ENSP00000251102.

Structurei

3D structure databases

ProteinModelPortaliQ14028.
SMRiQ14028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi568 – 578IQ-likeAdd BLAST11

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 63Glu-richAdd BLAST41
Compositional biasi171 – 252Pro-richAdd BLAST82
Compositional biasi351 – 371Poly-GluAdd BLAST21

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0499. Eukaryota.
ENOG410ZJ5U. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ14028.
KOiK04952.
OMAiWEDAHQD.
OrthoDBiEOG091G03EW.
PhylomeDBiQ14028.
TreeFamiTF318250.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032944. CNGB1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF386. PTHR10217:SF386. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: There is no evidence for an ortholog to bovine GARP1 in the human genome.
Isoform RCNC2B (identifier: Q14028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGWVQRVLP QPPGTPRKTK MQEEEEVEPE PEMEAEVEPE PNPEEAETES
60 70 80 90 100
ESMPPEESFK EEEVAVADPS PQETKEAALT STISLRAQGA EISEMNSPSR
110 120 130 140 150
RVLTWLMKGV EKVIPQPVHS ITEDPAQILG HGSTGDTGCT DEPNEALEAQ
160 170 180 190 200
DTRPGLRLLL WLEQNLERVL PQPPKSSEVW RDEPAVATGA ASDPAPPGRP
210 220 230 240 250
QEMGPKLQAR ETPSLPTPIP LQPKEEPKEA PAPEPQPGSQ AQTSSLPPTR
260 270 280 290 300
DPARLVAWVL HRLEMALPQP VLHGKIGEQE PDSPGICDVQ TISILPGGQV
310 320 330 340 350
EPDLVLEEVE PPWEDAHQDV STSPQGTEVV PAYEEENKAV EKMPRELSRI
360 370 380 390 400
EEEKEDEEEE EEEEEEEEEE EVTEVLLDSC VVSQVGVGQS EEDGTRPQST
410 420 430 440 450
SDQKLWEEVG EEAKKEAEEK AKEEAEEVAE EEAEKEPQDW AETKEEPEAE
460 470 480 490 500
AEAASSGVPA TKQHPEVQVE DTDADSCPLM AEENPPSTVL PPPSPAKSDT
510 520 530 540 550
LIVPSSASGT HRKKLPSEDD EAEELKALSP AESPVVAWSD PTTPKDTDGQ
560 570 580 590 600
DRAASTASTN SAIINDRLQE LVKLFKERTE KVKEKLIDPD VTSDEESPKP
610 620 630 640 650
SPAKKAPEPA PDTKPAEAEP VEEEHYCDML CCKFKHRPWK KYQFPQSIDP
660 670 680 690 700
LTNLMYVLWL FFVVMAWNWN CWLIPVRWAF PYQTPDNIHH WLLMDYLCDL
710 720 730 740 750
IYFLDITVFQ TRLQFVRGGD IITDKKDMRN NYLKSRRFKM DLLSLLPLDF
760 770 780 790 800
LYLKVGVNPL LRLPRCLKYM AFFEFNSRLE SILSKAYVYR VIRTTAYLLY
810 820 830 840 850
SLHLNSCLYY WASAYQGLGS THWVYDGVGN SYIRCYYFAV KTLITIGGLP
860 870 880 890 900
DPKTLFEIVF QLLNYFTGVF AFSVMIGQMR DVVGAATAGQ TYYRSCMDST
910 920 930 940 950
VKYMNFYKIP KSVQNRVKTW YEYTWHSQGM LDESELMVQL PDKMRLDLAI
960 970 980 990 1000
DVNYNIVSKV ALFQGCDRQM IFDMLKRLRS VVYLPNDYVC KKGEIGREMY
1010 1020 1030 1040 1050
IIQAGQVQVL GGPDGKSVLV TLKAGSVFGE ISLLAVGGGN RRTANVVAHG
1060 1070 1080 1090 1100
FTNLFILDKK DLNEILVHYP ESQKLLRKKA RRMLRSNNKP KEEKSVLILP
1110 1120 1130 1140 1150
PRAGTPKLFN AALAMTGKMG GKGAKGGKLA HLRARLKELA ALEAAAKQQE
1160 1170 1180 1190 1200
LVEQAKSSQD VKGEEGSAAP DQHTHPKEAA TDPPAPRTPP EPPGSPPSSP
1210 1220 1230 1240 1250
PPASLGRPEG EEEGPAEPEE HSVRICMSPG PEPGEQILSV KMPEEREEKA

E
Length:1,251
Mass (Da):139,678
Last modified:September 1, 2009 - v2
Checksum:i7A45CB399EB2B20C
GO
Isoform RCNC2A (identifier: Q14028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-628: Missing.

Show »
Length:623
Mass (Da):70,901
Checksum:i19FD335A37A9BD8A
GO
Isoform GARP2 (identifier: Q14028-3) [UniParc]FASTAAdd to basket
Also known as: GARP

The sequence of this isoform differs from the canonical sequence as follows:
     292-299: ISILPGGQ → RVMGAGGL
     300-1251: Missing.

Note: In the rod cells, the CNGB1 locus encodes the cyclic nucleotide-gated cation channel beta-1 subunit and several glutamic-acid-rich proteins (GARPs).
Show »
Length:299
Mass (Da):32,531
Checksum:i1B07296CA8ECB655
GO
Isoform 4 (identifier: Q14028-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-194: Missing.

Show »
Length:1,245
Mass (Da):139,179
Checksum:iE99F22376984493D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128I → V in AC010543 (PubMed:15616553).Curated1
Sequence conflicti1148 – 1149QQ → HE in AAB63387 (PubMed:7590744).Curated2
Sequence conflicti1148 – 1149QQ → HE in AAA65619 (PubMed:7682292).Curated2
Sequence conflicti1148 – 1149QQ → HE in AAA65620 (PubMed:7682292).Curated2
Sequence conflicti1207 – 1208RP → SC in AAB63387 (PubMed:7590744).Curated2
Sequence conflicti1207 – 1208RP → SC in AAA65619 (PubMed:7682292).Curated2
Sequence conflicti1207 – 1208RP → SC in AAA65620 (PubMed:7682292).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058691100R → H.3 PublicationsCorresponds to variant rs13336595dbSNPEnsembl.1
Natural variantiVAR_059225479L → I.Corresponds to variant rs2303783dbSNPEnsembl.1
Natural variantiVAR_059226535V → A.Corresponds to variant rs12927214dbSNPEnsembl.1
Natural variantiVAR_059227731N → K.Corresponds to variant rs376270dbSNPEnsembl.1
Natural variantiVAR_059228745L → I.Corresponds to variant rs10459809dbSNPEnsembl.1
Natural variantiVAR_059229911K → R.Corresponds to variant rs2303785dbSNPEnsembl.1
Natural variantiVAR_059230961A → S.Corresponds to variant rs16942445dbSNPEnsembl.1
Natural variantiVAR_060491993G → V in RP45. 1 PublicationCorresponds to variant rs121918532dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0011101 – 628Missing in isoform RCNC2A. CuratedAdd BLAST628
Alternative sequenceiVSP_053421189 – 194Missing in isoform 4. Curated6
Alternative sequenceiVSP_037921292 – 299ISILPGGQ → RVMGAGGL in isoform GARP2. 1 Publication8
Alternative sequenceiVSP_037922300 – 1251Missing in isoform GARP2. 1 PublicationAdd BLAST952

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15296 Genomic DNA. Translation: AAA65620.1.
L15297 Genomic DNA. Translation: AAA65619.1.
U18945 mRNA. Translation: AAA91633.1.
U58837 mRNA. Translation: AAB63387.1.
AF042498 mRNA. Translation: AAC04830.1.
AC010543 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82957.1.
CCDSiCCDS42169.1. [Q14028-1]
CCDS45495.1. [Q14028-3]
CCDS67042.1. [Q14028-4]
PIRiA57652.
S32538.
S69275.
RefSeqiNP_001129111.1. NM_001135639.1. [Q14028-3]
NP_001273059.1. NM_001286130.1. [Q14028-4]
NP_001288.3. NM_001297.4. [Q14028-1]
UniGeneiHs.147062.

Genome annotation databases

EnsembliENST00000251102; ENSP00000251102; ENSG00000070729. [Q14028-1]
ENST00000311183; ENSP00000311670; ENSG00000070729. [Q14028-3]
ENST00000564448; ENSP00000454633; ENSG00000070729. [Q14028-4]
GeneIDi1258.
KEGGihsa:1258.
UCSCiuc002emt.3. human. [Q14028-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15296 Genomic DNA. Translation: AAA65620.1.
L15297 Genomic DNA. Translation: AAA65619.1.
U18945 mRNA. Translation: AAA91633.1.
U58837 mRNA. Translation: AAB63387.1.
AF042498 mRNA. Translation: AAC04830.1.
AC010543 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82957.1.
CCDSiCCDS42169.1. [Q14028-1]
CCDS45495.1. [Q14028-3]
CCDS67042.1. [Q14028-4]
PIRiA57652.
S32538.
S69275.
RefSeqiNP_001129111.1. NM_001135639.1. [Q14028-3]
NP_001273059.1. NM_001286130.1. [Q14028-4]
NP_001288.3. NM_001297.4. [Q14028-1]
UniGeneiHs.147062.

3D structure databases

ProteinModelPortaliQ14028.
SMRiQ14028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107658. 3 interactors.
IntActiQ14028. 2 interactors.
MINTiMINT-4853474.
STRINGi9606.ENSP00000251102.

Protein family/group databases

TCDBi1.A.1.5.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ14028.
PhosphoSitePlusiQ14028.

Polymorphism and mutation databases

BioMutaiCNGB1.
DMDMi257051004.

Proteomic databases

EPDiQ14028.
PaxDbiQ14028.
PeptideAtlasiQ14028.
PRIDEiQ14028.

Protocols and materials databases

DNASUi1258.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000251102; ENSP00000251102; ENSG00000070729. [Q14028-1]
ENST00000311183; ENSP00000311670; ENSG00000070729. [Q14028-3]
ENST00000564448; ENSP00000454633; ENSG00000070729. [Q14028-4]
GeneIDi1258.
KEGGihsa:1258.
UCSCiuc002emt.3. human. [Q14028-1]

Organism-specific databases

CTDi1258.
DisGeNETi1258.
GeneCardsiCNGB1.
GeneReviewsiCNGB1.
H-InvDBHIX0038536.
HGNCiHGNC:2151. CNGB1.
HPAiHPA039159.
HPA060355.
MalaCardsiCNGB1.
MIMi600724. gene.
613767. phenotype.
neXtProtiNX_Q14028.
OpenTargetsiENSG00000070729.
Orphaneti791. Retinitis pigmentosa.
PharmGKBiPA26662.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0499. Eukaryota.
ENOG410ZJ5U. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ14028.
KOiK04952.
OMAiWEDAHQD.
OrthoDBiEOG091G03EW.
PhylomeDBiQ14028.
TreeFamiTF318250.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000070729-MONOMER.
ReactomeiR-HSA-2485179. Activation of the phototransduction cascade.
R-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-HSA-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

GeneWikiiCNGB1.
GenomeRNAii1258.
PROiQ14028.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000070729.
CleanExiHS_CNGB1.
ExpressionAtlasiQ14028. baseline and differential.
GenevisibleiQ14028. HS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032944. CNGB1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF386. PTHR10217:SF386. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNGB1_HUMAN
AccessioniPrimary (citable) accession number: Q14028
Secondary accession number(s): H3BN09
, O43636, Q13059, Q14029, Q9UMG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 1, 2009
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.