Q14011 (CIRBP_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 117.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cold-inducible RNA-binding protein Alternative name(s): A18 hnRNP Glycine-rich RNA-binding protein CIRP | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 172 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor By similarity. Promotes assembly of stress granules (SGs), when overexpressed. Ref.5 Ref.7 |
| Subunit structure | Interacts with EIF4G1. Associates with ribosomes. Ref.7 |
| Subcellular location | Nucleus › nucleoplasm. Cytoplasm. Note: Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins By similarity. Ref.5 |
| Tissue specificity | Ubiquitous. |
| Induction | By cold stress in response to DNA damage induced by UV irradiation or UV mimetic agents. Up-regulated by hypoxia. Ref.6 |
| Domain | Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression By similarity. |
| Post-translational modification | Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs By similarity. Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation. Ref.7 |
| Sequence similarities | Contains 1 RRM (RNA recognition motif) domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||
Molecule processing | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 172 | 172 | Cold-inducible RNA-binding protein | PRO_0000081503 | |||||||||||||||||||
Regions | |||||||||||||||||||||||
| Domain | 6 – 84 | 79 | RRM | ||||||||||||||||||||
| Compositional bias | 92 – 158 | 67 | Gly-rich | ||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||
| Beta strand | 6 – 12 | 7 | |||||||||||||||||||||
| Helix | 19 – 29 | 11 | |||||||||||||||||||||
| Beta strand | 34 – 37 | 4 | |||||||||||||||||||||
| Beta strand | 41 – 43 | 3 | |||||||||||||||||||||
| Beta strand | 48 – 53 | 6 | |||||||||||||||||||||
| Helix | 57 – 67 | 11 | |||||||||||||||||||||
| Beta strand | 78 – 83 | 6 | |||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene." Nishiyama H., Higashitsuji H., Yokoi H., Itoh K., Danno S., Matsuda T., Fujita J. Gene 204:115-120(1997) [PubMed: 9434172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Identification of several human homologs of hamster DNA damage-inducible transcripts. Cloning and characterization of a novel UV-inducible cDNA that codes for a putative RNA-binding protein." Sheikh M.S., Carrier F., Papathanasiou M.A., Hollander M.C., Zhan Q., Yu K., Fornace A.J. Jr. J. Biol. Chem. 272:26720-26726(1997) [PubMed: 9334257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M.Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Placenta. |
| [5] | "The UV-inducible RNA-binding protein A18 (A18 hnRNP) plays a protective role in the genotoxic stress response." Yang C., Carrier F. J. Biol. Chem. 276:47277-47284(2001) [PubMed: 11574538] [Abstract] Cited for: FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION. |
| [6] | "Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism." Wellmann S., Buehrer C., Moderegger E., Zelmer A., Kirschner R., Koehne P., Fujita J., Seeger K. J. Cell Sci. 117:1785-1794(2004) [PubMed: 15075239] [Abstract] Cited for: INDUCTION BY HYPOXIA. |
| [7] | "Post-transcriptional regulation of thioredoxin by the stress inducible heterogenous ribonucleoprotein A18." Yang R., Weber D.J., Carrier F. Nucleic Acids Res. 34:1224-1236(2006) [PubMed: 16513844] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, RNA-BINDING, INTERACTION WITH EIF4G1, ASSOCIATION WITH RIBOSOMES. |
| [8] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [9] | "Solution structure of RRM domain in A18 HNRNP." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-90. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | D78134 mRNA. Translation: BAA11212.1. AF021336 mRNA. Translation: AAC51787.1. AC004258 Genomic DNA. Translation: AAC04895.1. BC000403 mRNA. Translation: AAH00403.1. BC000901 mRNA. Translation: AAH00901.1. | ||||||||||||
| IPI | IPI00641579. | ||||||||||||
| RefSeq | NP_001271.1. NM_001280.2. | ||||||||||||
| UniGene | Hs.618145. Hs.634522. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q14011. | ||||||||||||
| SMR | Q14011. Positions 1-90. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | Q14011. 8 interactions. | ||||||||||||
| MINT | MINT-5004534. | ||||||||||||
| STRING | Q14011. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q14011. | ||||||||||||
Polymorphism databases | |||||||||||||
| DMDM | 5921786. | ||||||||||||
2D gel databases | |||||||||||||
| REPRODUCTION-2DPAGE | IPI00180954. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | Q14011. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000320936; ENSP00000322887; ENSG00000099622. | ||||||||||||
| GeneID | 1153. | ||||||||||||
| KEGG | hsa:1153. | ||||||||||||
| UCSC | uc002lrr.2. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 1153. | ||||||||||||
| GeneCards | GC19P001269. | ||||||||||||
| H-InvDB | HIX0014577. | ||||||||||||
| HGNC | HGNC:1982. CIRBP. | ||||||||||||
| MIM | 602649. gene. | ||||||||||||
| neXtProt | NX_Q14011. | ||||||||||||
| PharmGKB | PA26519. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | prNOG21015. | ||||||||||||
| HOVERGEN | HBG107480. | ||||||||||||
| InParanoid | Q14011. | ||||||||||||
| OMA | AGKSSEN. | ||||||||||||
| OrthoDB | EOG4HHP40. | ||||||||||||
| PhylomeDB | Q14011. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q14011. | ||||||||||||
| Bgee | Q14011. | ||||||||||||
| CleanEx | HS_CIRBP. | ||||||||||||
| Genevestigator | Q14011. | ||||||||||||
| GermOnline | ENSG00000099622. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit. | ||||||||||||
| KO | K13195. | ||||||||||||
| Pfam | PF00076. RRM_1. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00360. RRM. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50102. RRM. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 4788. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | CIRBP_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q14011 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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