Reviewed, UniProtKB/Swiss-Prot Q13Z15 (CLPP2_BURXL)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 2
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	Bxeno_A2136
ORF Names:	Bxe_A2295

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000252809
Sites
	Active site	121	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13Z15-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F39A8F403B3238B7	FASTA	217	23,829
	10 20 30 40 50 60 MTFRAQMLDT LTSQSSRDLE AQALGLVPIV VETSGRGERS YDIYSRLLKE RIVFLVGEVN 70 80 90 100 110 120 DQTANLVVAQ MLFLESENPD KDISFYINSP GGSVSAGMAI YDTMQFIKPD VSTLCMGLAA 130 140 150 160 170 180 SMGAFLLAAG AKGKRFALPN SRVMIHQPLG GARGQASDIE IQAREILYLK ERLNHLLAHH 190 200 210 TGQPVERIAR DTDRDNFMSG DDAQAYGLVD QVAHKRP

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE30674.1.
RefSeq	YP_558726.1.
3D structure databases
SMR	Q13Z15. Positions 26-216.
ModBase	Search...
Protein family/group databases
MEROPS	S14.001.
Genome annotation databases
GeneID	4005027.
GenomeReviews	Gene locus Bxeno_A2136 in contig CP000270_GR.
KEGG	bxe:Bxe_A2295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	ILIQARE.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. clpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_BURXL

Accession

Primary (citable) accession number: Q13Z15

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)