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Protein

Malate synthase G

Gene

glcB

Organism
Burkholderia xenovorans (strain LB400)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB), Malate synthase (Bxe_A1647)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei633Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Bxeno_A2208
ORF Names:Bxe_A2223
OrganismiBurkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001817 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569001 – 724Malate synthase GAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei619Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266265.Bxe_A2223.

Structurei

3D structure databases

ProteinModelPortaliQ13YU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiGYNEVRG.
OrthoDBiPOG091H05P0.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q13YU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQMNPRGGL QVAANLDQFV ETEALPGTGL DSAAFWSGFD ALVHELAPKN
60 70 80 90 100
RALLAERDRL QTELDNWHRA NPGPVRDLRA YRAFLEGIGY IVPVPASVKA
110 120 130 140 150
TTDHVDTEIA EQAGPQLVVP LSNQRYALNA ANARWGSLYD ALYGTDAIPE
160 170 180 190 200
ANGAEKQKAF NPVRGAAVIA YARRFLDQAA PLANGSHADA TRYGVEGGKL
210 220 230 240 250
VVTLKNGTSE LKTPAQFIGY QGEESAPSAV LLKHNGLHFE IQIDANDSIG
260 270 280 290 300
KTDSAHVKDV VVEAAVSTII DCEDSVAAVD ADDKVQLYRN WLGLMNGDLT
310 320 330 340 350
EEVTKNGKTF TRRLNADRVY TAANGTAPVV LHGRSLLFIR NVGHLMTNPA
360 370 380 390 400
VLTKDGHEIP EGILDAVITS LCALHDRKHK LNSRTGSIYI VKPKMHGPAE
410 420 430 440 450
VAFASELFAR VEDLLKLPRN TIKMGIMDEE RRTSVNLLAC INEARERVAF
460 470 480 490 500
INTGFLDRTG DEMHTAMEAG PMLRKGDMKS SAWIAAYERS NVLVGLSAGL
510 520 530 540 550
RGRSQIGKGM WAMPDLMHAM LEQKIAHPKA GANTAWVPSP TAATLHALHY
560 570 580 590 600
HQVDVQAVQQ ELERTDYAKV RDELLDGLLT IPVVAEAKWS DDEIRSEIDN
610 620 630 640 650
NAQGILGYVV RWIDQGVGCS KVPDIHNVGL MEDRATLRIS SQHIANWLYH
660 670 680 690 700
GVVKRELVEE TFRRMARVVD EQNAGDPLYK PMAPGFDTIA FKAAQALVFE
710 720
GRQQPSGYTE PLLHKFRLEV KKEA
Length:724
Mass (Da):79,450
Last modified:August 22, 2006 - v1
Checksum:i342CB47D2034DB90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000270 Genomic DNA. Translation: ABE30746.1.
RefSeqiWP_011488362.1. NZ_CP008760.1.

Genome annotation databases

EnsemblBacteriaiABE30746; ABE30746; Bxe_A2223.
GeneIDi4003121.
KEGGibxb:DR64_4375.
bxe:Bxe_A2223.
PATRICi19329978. VBIBurXen52548_2309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000270 Genomic DNA. Translation: ABE30746.1.
RefSeqiWP_011488362.1. NZ_CP008760.1.

3D structure databases

ProteinModelPortaliQ13YU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266265.Bxe_A2223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE30746; ABE30746; Bxe_A2223.
GeneIDi4003121.
KEGGibxb:DR64_4375.
bxe:Bxe_A2223.
PATRICi19329978. VBIBurXen52548_2309.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiGYNEVRG.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_BURXL
AccessioniPrimary (citable) accession number: Q13YU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: November 30, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.