Q13YI8 (PHNX_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	Bxeno_A2313
ORF Names:	Bxe_A2117

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia xenovorans

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity. HAMAP-Rule MF_01375
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity. HAMAP-Rule MF_01375
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01375
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_0000284587
Sites
	Active site	10	1	Nucleophile By similarity
	Active site	51	1	Schiff-base intermediate with substrate By similarity
	Metal binding	10	1	Magnesium By similarity
	Metal binding	12	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	184	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13YI8 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: AB50CDAD236F0F83	FASTA	267	28,358
	10 20 30 40 50 60 MKHVKAVIFD WAGTVVDYGS LAPMGAFVET FGQFGVPITI DEARGPMGMA KRPHIAALMA 70 80 90 100 110 120 LPRVAQAWTD KYGHAPDDAD IDAVYDVFVP KNIAVAASYS SVIPGVADVA SALRGDDIRI 130 140 150 160 170 180 GTTTGYTREI MAEIVPGAAA QGFSPDSIVC TGDTAEGRPS PYMIYRTLPE LGVWRAKEAI 190 200 210 220 230 240 KVDDTEVGIE EGINGGTWTV GVSVSGNAFG MAEGDVKALA PDEFAWRRNA AIQKLQAAGA 250 260 HYVIDSVADL MPVVYDIEAR LARGERP

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE30851.1.
RefSeq	YP_558903.1. NC_007951.1.
3D structure databases
ProteinModelPortal	Q13YI8.
ModBase	Search...
Protein-protein interaction databases
STRING	266265.Bxe_A2117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE30851; ABE30851; Bxe_A2117.
GeneID	4005089.
KEGG	bxe:Bxe_A2117.
PATRIC	19330202. VBIBurXen52548_2421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	BXEN266265:GJII-2415-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_BURXL

Accession

Primary (citable) accession number: Q13YI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program