Q13XC6 (LPXD_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Bxeno_A2725
ORF Names:	Bxe_A1692

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264354
Sites
	Active site	252	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13XC6 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 1ECF43FF8D121565	FASTA	370	38,082
	10 20 30 40 50 60 MAFTLEDIVQ RFGGEVVGNG SQRVGSLAPL DQAGPDQLAF LANPKYLSQV ETTRAGAVLI 70 80 90 100 110 120 NAEDLARLAS RESRNFIVTP NPYAYFARVA QTFIDLAAPK AVPGVHPSAT IDPSAQIAAS 130 140 150 160 170 180 AVIGPHVTVE AGAVIGENVR LDANVVIGRG TRIGADSHLY PNVAVYYGCK LGERVIVHAG 190 200 210 220 230 240 AVIGSDGFGF APDFVGEGDA RTGSWVKIPQ VGGVSIAADV EIGANTTIDR GAMADTIIEE 250 260 270 280 290 300 CVKIDNLVQI GHNCKVGAYT VIAGCAGIAG STTIGRHCMI GGAVGIAGHV TLADYVIVTA 310 320 330 340 350 360 KSGVSKSLLK PGMYTSAFPA VNHADWNKSA ALLRNIDKLR DRIKALENAA AGRQDGPAAN 370 AASSSAGDKA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE31263.1.
RefSeq	YP_559315.1. NC_007951.1.
3D structure databases
ProteinModelPortal	Q13XC6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13XC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4005007.
GenomeReviews	Gene locus Bxeno_A2725 in contig CP000270_GR.
KEGG	bxe:Bxe_A1692.
PATRIC	19331070. VBIBurXen52548_2855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q13XC6.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BURXL

Accession

Primary (citable) accession number: Q13XC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program