Q13VU0 (MHPD_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-keto-4-pentenoate hydratase
EC=4.2.1.80

Alternative name(s):
2-hydroxypentadienoic acid hydratase

Gene names

Name:	mhpD
Ordered Locus Names:	Bxeno_A3261
ORF Names:	Bxe_A1149

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia xenovorans

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid By similarity. HAMAP-Rule MF_01655
Catalytic activity	4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O. HAMAP-Rule MF_01655
Cofactor	Divalent metal ions By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01655
Sequence similarities	Belongs to the hydratase/decarboxylase family. MhpD subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	3-phenylpropionate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-oxopent-4-enoate hydratase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	2-keto-4-pentenoate hydratase HAMAP-Rule MF_01655		PRO_0000337789

Sequences

	Sequence		Length	Mass (Da)
	Q13VU0 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 9A9B4B8A3B95CFF0	FASTA	269	28,759
	10 20 30 40 50 60 MTPQQREEAA QSLYQAMQSG KPIAPLRDTF PDMNVDDAYA IQSINTQRRI SLGRRVVGRK 70 80 90 100 110 120 IGLTSVVVQQ QLGVDEPDFG ALFDDMSFGD AETIPLSILH QPKVEAEIGF VLGRDLDTEQ 130 140 150 160 170 180 PTHQEVLQAV DYVVPALEIV GSRIADWNIK FVDTVADNAS SGVYVLGSTP ISPRGLDLSL 190 200 210 220 230 240 VGMCLSRRGE PVSTGAGAAC LGTPLNAVVW LARTMSRLGK PLRAGELILS GALGPMVAVK 250 260 PGDVFECHIN GVGSVRTEFE SNQMNGVAA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE31799.1.
RefSeq	YP_559851.1. NC_007951.1.
3D structure databases
ProteinModelPortal	Q13VU0.
SMR	Q13VU0. Positions 1-261.
ModBase	Search...
Protein-protein interaction databases
STRING	266265.Bxe_A1149.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE31799; ABE31799; Bxe_A1149.
GeneID	4002976.
KEGG	bxe:Bxe_A1149.
PATRIC	19332248. VBIBurXen52548_3428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3971.
HOGENOM	HOG000179637.
KO	K02554.
OMA	CKIGLTS.
ProtClustDB	CLSK753177.
Enzyme and pathway databases
BioCyc	BXEN266265:GJII-3393-MONOMER.
SABIO-RK	Q13VU0.
UniPathway	UPA00714.
Family and domain databases
Gene3D	3.90.850.10. 1 hit.
HAMAP	MF_01655. MhpD.
InterPro	IPR002529. Fumarylacetoacetase_C. IPR011234. Fumarylacetoacetase_C-rel. IPR023793. Keto_pentenoate-hydratase. [Graphical view]
Pfam	PF01557. FAA_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56529. Fumarylacetoacetase_C-rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPD_BURXL

Accession

Primary (citable) accession number: Q13VU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program