Reviewed, UniProtKB/Swiss-Prot Q13VE3 (DADA_BURXL)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Bxeno_A3408
ORF Names:	Bxe_A1001

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H(2)O + acceptor = a 2-oxo acid + NH(3) + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1.
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	D-amino acid dehydrogenase small subunit		PRO_1000066086
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q13VE3-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: CDB9833D372207EB	FASTA	429	46,194
	10 20 30 40 50 60 MRVVVLGSGV VGVTSAYYLA RAGHEVTVID REAGPALETS FANAGQISPG YASPWAAPGV 70 80 90 100 110 120 PLKAVKWMFQ KHAPLAIRLD GTQFQLQWMW QMLQNCTSSR YAVNKGRMVR LAEYSRDCLQ 130 140 150 160 170 180 ALRAETGIQY EGRTGGTLQV FRTQQQFEGA AKDIAVLREA SVPYELLSPA ELAQAEPALA 190 200 210 220 230 240 AVSHKLTGGL RLPGDETGDC QMFTTRLAAL AEQLGVKFRY NTPIDALAMA GDRIAGVKCG 250 260 270 280 290 300 EELVRADSFV VALGSYSTQF LSGLVKIPVY PLKGYSITAP IVNEASAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDDRIRV GGMAEIVGFD KSLREARRET LELCVNDLFP GGGDTSKATF WSGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPV ANLFLNTGHG TLGWTMSCGS GQLLADVMSG KQPAIKADDL SVHRYLGETR GAHRPAYAA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE31946.1.
RefSeq	YP_559998.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4003991.
GenomeReviews	Gene locus Bxeno_A3408 in contig CP000270_GR.
KEGG	bxe:Bxe_A1001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q13VE3.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_BURXL

Accession

Primary (citable) accession number: Q13VE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	November 4, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)