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Q13V14 (TRMB_BURXL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Bxeno_A3537
ORF Names:Bxe_A0859
OrganismBurkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]
Taxonomic identifier266265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence ABE32075.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288131

Regions

Region236 – 2394Substrate binding By similarity

Sites

Active site1651 By similarity
Binding site901S-adenosyl-L-methionine By similarity
Binding site1151S-adenosyl-L-methionine By similarity
Binding site1421S-adenosyl-L-methionine By similarity
Binding site1651S-adenosyl-L-methionine By similarity
Binding site1691Substrate By similarity
Binding site2011Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q13V14 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 433D83F9E3C279D8

FASTA26029,078
        10         20         30         40         50         60 
MIHDPNDAGL PDQLPTPSSE AENSPAGDTT PPEEALHRRR IRSFVTRAGR VSTGQRRAMD 

        70         80         90        100        110        120 
ELGPRFVVPF APQQPDWNSV FGREAPRVLE IGFGMGATTA EIASHRPDED FLGVEVHEPG 

       130        140        150        160        170        180 
VGALLKLMGE QNLSNIRIIQ HDAVEVLEQM IAPDSLDGVH IFFPDPWHKA RHHKRRLIQP 

       190        200        210        220        230        240 
KFVALLVSRL KPGAYLHCAT DWQNYAEQML EVLGAEPSLK NTADGYAPRP EYRPVTKFER 

       250        260 
RGLRLGHGVW DLVFRKRGAG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000270 Genomic DNA. Translation: ABE32075.1. Different initiation.
RefSeqYP_560127.1. NC_007951.1.

3D structure databases

ProteinModelPortalQ13V14.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266265.Bxe_A0859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE32075; ABE32075; Bxe_A0859.
GeneID4002874.
KEGGbxe:Bxe_A0859.
PATRIC19332855. VBIBurXen52548_3723.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAQDNFLCC.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycBXEN266265:GJII-3689-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BURXL
AccessionPrimary (citable) accession number: Q13V14
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 11, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways