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Q13UW2

- SYI_BURXL

UniProt

Q13UW2 - SYI_BURXL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia xenovorans (strain LB400)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylateUniRule annotation
Binding sitei625 – 6251ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBXEN266265:GJII-3742-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bxeno_A3589
ORF Names:Bxe_A0807
OrganismiBurkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001817: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligasePRO_1000022051Add
BLAST

Proteomic databases

PRIDEiQ13UW2.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266265.Bxe_A0807.

Structurei

3D structure databases

ProteinModelPortaliQ13UW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTALWQIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13UW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNKKADSKP QSRYPVNLLD TPFPMRGDLP KREPQWVKEW QERKVYETIR
60 70 80 90 100
AASKGRKKFI LHDGPPYANG DIHLGHAVNK ILKDMIVKAR NLAGFDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMQK ARAYATEQIE KQKVGFRRLG
160 170 180 190 200
VLGDWDNPYK TMNFTNEAGE IRALAKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK TDPTIDVLFS FAEPEKTAQA FGLNALPRNE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTSRGLLILA QERVEACLKQ YGLEGTIVAT
310 320 330 340 350
TPGAKLVNLR FNHPLASAHP SYKRTSPVYL GDYVTTESGT GVVHSSPAYG
360 370 380 390 400
VEDFVSCKAH GMSDSDIINP VMGDGRYIES LALFGGLSIW AANPQIVEAL
410 420 430 440 450
QGAGTLMRTE NYTHSYMHCW RHKTPIIYRA TSQWFAGMDV KPNDTDRTLR
460 470 480 490 500
ETALEGIENT AFYPAWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAQRVE KAGIEAWQSL DPRELLGDEA NMYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD ELQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRPPYNALL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD FAQHARPVED
710 720 730 740 750
WLEIDRYAVA LSANLQNDIL SHYEKYEFHP VVAKLQTFCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTTAA DSVARRAAQT ALYHIAHGLL RLMAPFLSFT AEEAWKIFEP
810 820 830 840 850
NSETIFTETY HAFPAVPEAG ALLDKWTLLR AARSDVTKAL EEARVANLIG
860 870 880 890 900
SSLQAEVEIR ASGARYDALT SLGDDLKFVL ITSAASVVKV DSEAEEGVEV
910 920 930 940
IASKYLKCER CWHYRADVGA NAEHPTLCGR CFSNLFGNGE TRSAA
Length:945
Mass (Da):106,008
Last modified:August 22, 2006 - v1
Checksum:i2E23E36949D06FFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000270 Genomic DNA. Translation: ABE32127.1.
RefSeqiYP_560179.1. NC_007951.1.

Genome annotation databases

EnsemblBacteriaiABE32127; ABE32127; Bxe_A0807.
GeneIDi4003236.
KEGGibxe:Bxe_A0807.
PATRICi19332969. VBIBurXen52548_3779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000270 Genomic DNA. Translation: ABE32127.1 .
RefSeqi YP_560179.1. NC_007951.1.

3D structure databases

ProteinModelPortali Q13UW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266265.Bxe_A0807.

Proteomic databases

PRIDEi Q13UW2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE32127 ; ABE32127 ; Bxe_A0807 .
GeneIDi 4003236.
KEGGi bxe:Bxe_A0807.
PATRICi 19332969. VBIBurXen52548_3779.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi TALWQIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BXEN266265:GJII-3742-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LB400.

Entry informationi

Entry nameiSYI_BURXL
AccessioniPrimary (citable) accession number: Q13UW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3