Q13UR0 (MURB_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Bxeno_A3641
ORF Names:	Bxe_A0755

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000332451
Regions
	Domain	22 – 194	173	FAD-binding PCMH-type
Sites
	Active site	170	1	By similarity
	Active site	246	1	Proton donor By similarity
	Active site	342	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13UR0 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: BFAF0255E860264C	FASTA	346	37,310
	10 20 30 40 50 60 MSQTESAAFL AGYPLKAHNT FGFDVRARFA CRIEQEAQLM PAVRDPRAAG LPRLVLGGGS 70 80 90 100 110 120 NVVLTGDFGG LVLLVALRGR RVVREDNDAW YVEAAGGEPW HEFVGWTLSQ GMAGLENLAL 130 140 150 160 170 180 IPGTVGAAPI QNIGAYGLEM CERFASLRAV ELTTGAVVEL DAQACRFGYR DSFFKREGRD 190 200 210 220 230 240 RFVITSVTFR LPKVWQPRAG YADLARELAA NGHAGTPPTA QAIFDAVVAV RRAKLPDPLE 250 260 270 280 290 300 LGNAGSFFKN PVIGPAQFEA LKLREPDVVS YVQADGRVKL AAGWLIDRCG WKGRAMGAAA 310 320 330 340 VHERQALVLV NRGGASGAEV LALAKAIQRD VRERFGVELE PEPVCL

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE32179.1.
RefSeq	YP_560231.1. NC_007951.1.
3D structure databases
ProteinModelPortal	Q13UR0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13UR0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4005636.
GenomeReviews	Gene locus Bxeno_A3641 in contig CP000270_GR.
KEGG	bxe:Bxe_A0755.
PATRIC	19333085. VBIBurXen52548_3837.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	IEQAGWK.
PhylomeDB	Q13UR0.
ProtClustDB	PRK00046.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BURXL

Accession

Primary (citable) accession number: Q13UR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program