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Q13U82 (SYL_BURXL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bxeno_A3819
ORF Names:Bxe_A0576
OrganismBurkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]
Taxonomic identifier266265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009314

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif623 – 6275"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6261ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q13U82 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: C4CA8F1BAC7F1069

FASTA86396,985
        10         20         30         40         50         60 
MHEKYVPSDV ESAAQGQWRA IDAYKTTEVT DKPKFYCVSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
VMYRYLRMNG YNVLMPMGWD AFGMPAENAA MANNVPPAKW TYDNIAYMKK QMQSMGLAID 

       130        140        150        160        170        180 
WSREVATCSP DYYKWNQWLF LKMLEKGIAY KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKREIPM YYMRITQYAD ELLNDLEGLG WPERVKIMQQ NWIGKSFGVN FGFPYEIDGE 

       250        260        270        280        290        300 
QKLLRVFTTR ADTIMGVTFC AIAAEHPLAT RLAKDKPELQ AFIEECKRGG VAEADVATME 

       310        320        330        340        350        360 
KKGMATGFTV THPLTQEQVE VWIGNYVLMS YGEGAVMGVP AHDERDFAFV KKYGLPVRQV 

       370        380        390        400        410        420 
VAVEGKEFST EAWQEWYGEK TGTLINSGKY DGLNYEQAVD RIAADLKELG LGDKQITWRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGTPIPIIHC PTCGDVPVPE KDLPVVLPED LVPDGTGNPL ARSEAFVNCT 

       490        500        510        520        530        540 
CPTCGGAAKR ETDTMDTFVD SSWYFYRYAS PGAKTMVDER TDYWAPMDQY IGGIEHAILH 

       550        560        570        580        590        600 
LLYSRFWAKV MRDLGLIRFG EPAKNLLTQG MVLNETYYRE NEAGKKTWYN PAEVTVSFDD 

       610        620        630        640        650        660 
KGRPVGAILN EDGQPVVLGG VEKMSKSKNN GVDPQLLIDQ HGADTARLFV MFAAPPEQSL 

       670        680        690        700        710        720 
EWSGSGVEGA SRFLRRVWSF SQANEAALRQ GGTFDAAQLS DVEKVLRREI YSVLKQADFD 

       730        740        750        760        770        780 
YQRLQYNTVV SAAMKMLNAL DSAKGARPAV LRETCSVMLR VLYPVVPHLT FQLWQELGYA 

       790        800        810        820        830        840 
DELGSLLDAP WPKVDEKALE QSEIELVLQV NGKVRGAITV AKDASREAIE QLAAAHEMVA 

       850        860 
KFSEGKAPKK IVVVPGRLVN VVV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000270 Genomic DNA. Translation: ABE32357.1.
RefSeqYP_560409.1. NC_007951.1.

3D structure databases

ProteinModelPortalQ13U82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266265.Bxe_A0576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE32357; ABE32357; Bxe_A0576.
GeneID4005835.
KEGGbxe:Bxe_A0576.
PATRIC19333483. VBIBurXen52548_4035.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBXEN266265:GJII-3974-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BURXL
AccessionPrimary (citable) accession number: Q13U82
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries