Reviewed, UniProtKB/Swiss-Prot Q13TZ0 (MURD_BURXL)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Bxeno_A3911
ORF Names:	Bxe_A0484

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257174
Regions
	Nucleotide binding	132 – 138	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q13TZ0-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 472B1B4FC3FBD3FF	FASTA	504	53,592
	10 20 30 40 50 60 MFGEKFRDRQ KPMVLVLGLG ESGLAMARWC ARHGCRLRAA DTREVPPNLS ALEAHGVDAQ 70 80 90 100 110 120 FVGGPFSPVL LEGVELVAIS PGLSPLAADL LPLIAAAREQ GIPVWGELEF FSQALKTLGE 130 140 150 160 170 180 SGYAPKVIAI TGTNGKTTTT SLTGLLCERA GRKVAVAGNI SPALLDKLSE AIDNTALPDV 190 200 210 220 230 240 WVLELSSFQL ETAHTFSPDA AVVLNITQDH LDWHGGLDAY AAAKGRIFGT QTVRVLNRDD 250 260 270 280 290 300 SRVMKLAPSE DSEAGMVTFG VTEPKNEGDY GLLRDNGMIW LVEAHDRDAS DEPAPKRRRK 310 320 330 340 350 360 NEVATPPNIA LKRLMPADAL RIRGLHNAAN ALAAYALARA IGLPGASLLH GLREYRGEPH 370 380 390 400 410 420 RVELIASIDG IDYVDDSKGT NVGATVAALD GLAQRVVLIA GGDGKGQDFE PLAAPVMRWC 430 440 450 460 470 480 RAVMLIGRDA PQIRAALEDT GIAMTDHATL EEATRAAAAL AQPGDAVLLS PACASFDMFK 490 500 GYAHRAAVFR GTVEEIAAER GTMI

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE32449.1.
RefSeq	YP_560501.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4005928.
GenomeReviews	Gene locus Bxeno_A3911 in contig CP000270_GR.
KEGG	bxe:Bxe_A0484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q13TZ0.
OMA	SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURXL

Accession

Primary (citable) accession number: Q13TZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)