Q13S07 (GLGB_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	Bxeno_B0164
ORF Names:	Bxe_B2863

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000260641
Sites
	Active site	415	1	Nucleophile By similarity
	Active site	470	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13S07 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 4D7D9682B44E3CAC	FASTA	736	82,135
	10 20 30 40 50 60 MSEHDPAAGL QPLDIDALVE ARHPDPFSQL GLHHTDAGPV VRALLPNAAH VSVISRADGA 70 80 90 100 110 120 LLGELEQLRP GLFAGRITSA APYRLRIDWH GVVQEIEDTY SFGPVLGDEP LGRLAGGDPY 130 140 150 160 170 180 AVLECLGARP MEVDGVPGVR FAVWAPNARR VSVVGDFNAW DGRRHPMRLR HQAGVWELFV 190 200 210 220 230 240 PRVGPGTRYK YELLSRDGHP LPLKADPCAM QTEKPPGTAS IVAHVDEVEQ FPWSDHEWIQ 250 260 270 280 290 300 SRAGKQTARS PISIYEVHAE SWLRVAEEGQ RGLDWEELAE RMIPYVKSMG FTHVEFMPIA 310 320 330 340 350 360 EHPFGGSWGY QPLGQFAPSA RFGKPEQFAR FVDKAHEAGL GVILDWVPAH FPNDAHGLID 370 380 390 400 410 420 FDGTPLYEHA DPREGYHQDW NTMIYNLGRN EVSAFLIASG LAWLKRYHVD GLRVDAVASM 430 440 450 460 470 480 LYRDYSRAAD QWVPNIYGGR ENLESIAFLK RLNHEVGYVP GVPGAITIAE ESTAWPGVTA 490 500 510 520 530 540 RVEDGGLGFQ FKWNMGWMHD TLHYMEEDPV YRQYHHHNMT FGMVYAYSER FVLPLSHDEV 550 560 570 580 590 600 VHGKGSLLGK MPGDRWQKFA NLRAYFGFMW THPGKKLLFM GGEFGQLAEF NHDASPHWHL 610 620 630 640 650 660 LDDSNHHGVQ MLVRDLNRLY SDEPALYLLD CEPGGFEWLI GDDSGNSVFA YRRTDGAGRE 670 680 690 700 710 720 LVVVCNMTPV PRLGYRIGMP RGGRWVEVLN TDAGVYGGSN MGNGGLIHTD SQSSHGWPHS 730 AALTLPPLAT IVLRAD

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000271 Genomic DNA. Translation: ABE33132.1.
RefSeq	YP_552482.1. NC_007952.1.
3D structure databases
ProteinModelPortal	Q13S07.
SMR	Q13S07. Positions 124-736.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13S07.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4006772.
GenomeReviews	Gene locus Bxeno_B0164 in contig CP000271_GR.
KEGG	bxe:Bxe_B2863.
PATRIC	19335106. VBIBurXen52548_4828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	Q13S07.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B2863-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURXL

Accession

Primary (citable) accession number: Q13S07

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program