Reviewed, UniProtKB/Swiss-Prot Q13RB8 (XYLA_BURXL)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	Bxeno_B0403
ORF Names:	Bxe_B2622

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Xylose isomerase HAMAP MF_00455		PRO_1000026437
Sites
	Active site	100	1	By similarity
	Active site	103	1	By similarity
	Metal binding	231	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 2 By similarity
	Metal binding	270	1	Magnesium 2 By similarity
	Metal binding	295	1	Magnesium 1 By similarity
	Metal binding	306	1	Magnesium 2 By similarity
	Metal binding	308	1	Magnesium 2 By similarity
	Metal binding	338	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13RB8-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 65BE1A8AB9BE1914	FASTA	440	49,775
	10 20 30 40 50 60 MSYFEHIPEI RYEGPQSDNP LAYRHYDKSK KVLGKTLEEH LRIAVCYWHT FVWPGVDIFG 70 80 90 100 110 120 QGTFRRPWQQ AGDAMERARQ KADSAFEFFS KLGTPYYTFH DTDVSPEGSS LKEYSENFLR 130 140 150 160 170 180 ITDYLARKQE DTGIKLLWGT ANLFSHPRYA AGAATSPDPE VFAFAATQVR HALDATQRLG 190 200 210 220 230 240 GDNYVLWGGR EGYDTLLNTD LMRERDQLAR FLHMVVDHAH KIGFKGSLLI EPKPQEPTKH 250 260 270 280 290 300 QYDYDVATVH GFLLQHGLEK EIRVNIEANH ATLAGHSFHH EIATAYALGI FGSVDANRGD 310 320 330 340 350 360 PQNGWDTDQF PNSVEELTLA FYEILKHGGF TTGGMNFDSK VRRQSVDPED LFYGHIGAID 370 380 390 400 410 420 NLALAVERAA VLIENDRLDQ FKRQRYAGWD ADFGRKILSG DYSLSTLATD ALARGLNPQH 430 440 ASGHQELMEN IVNQAIYSGR

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000271 Genomic DNA. Translation: ABE33371.1.
RefSeq	YP_552721.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4006662.
GenomeReviews	Gene locus Bxeno_B0403 in contig CP000271_GR.
KEGG	bxe:Bxe_B2622.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q13RB8.
OMA	QFLIEPK.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B2622-MON.
Family and domain databases
HAMAP	MF_00455. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. IPR018115. Xylose_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_BURXL

Accession

Primary (citable) accession number: Q13RB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)