Reviewed, UniProtKB/Swiss-Prot Q13QU8 (F16A2_BURXL)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 2
      Short name=FBPase class 1 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Ordered Locus Names:	Bxeno_B0573
ORF Names:	Bxe_B2450

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000364507
Regions
	Region	123 – 126	4	Substrate binding By similarity
Sites
	Metal binding	98	1	Magnesium 1 By similarity
	Metal binding	120	1	Magnesium 1 By similarity
	Metal binding	120	1	Magnesium 2 By similarity
	Metal binding	122	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	123	1	Magnesium 2 By similarity
	Metal binding	299	1	Magnesium 2 By similarity
	Binding site	227	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13QU8-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 01910B54CF45B627	FASTA	378	41,346
	10 20 30 40 50 60 MQDGRTTLSK FLIDTLDRQP CSTETARNAG LSALLIDVAA AIKSISAMLT KGALGGNYGS 70 80 90 100 110 120 AQSINTHGEE QKKLDVATNE IFVQQCEWDG LLAAMVSEEM ESVYAIPPGY PRGDYLLAFD 130 140 150 160 170 180 PLDGSSNIDI NGVVGSIFSV LRNGEGNGNA NEKNGSDPRG TVGESAFLRP GCEQVAAGYA 190 200 210 220 230 240 VYGPSTMLVL SVGNGTHGFT LEREIGNFVL THSNIRIPED TVEFAINASN ERFWEPPVRR 250 260 270 280 290 300 YVQECKDGRS GCRASDFNMR WIASMVAEVH RILMRGGVFM YPRDSKTPAM EGRLRLLYEA 310 320 330 340 350 360 NPMSFLVEQA GGLSITGRER ILDVVPRALH GRVPVILGSK HEVERIGRYH GEYDRGEDQP 370 FTSPLFSRRS LFLPGFTA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000271 Genomic DNA. Translation: ABE33541.1.
RefSeq	YP_552891.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4007054.
GenomeReviews	Gene locus Bxeno_B0573 in contig CP000271_GR.
KEGG	bxe:Bxe_B2450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q13QU8.
OMA	IFLYPGN.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B2450-MON.
Family and domain databases
HAMAP	MF_01855. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_BURXL

Accession

Primary (citable) accession number: Q13QU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)