ID   MHPB_BURXL              Reviewed;         316 AA.
AC   Q13QH9;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase;
DE            EC=1.13.11.16;
GN   Name=mhpB; OrderedLocusNames=Bxeno_B0692; ORFNames=Bxe_B2328;
OS   Burkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=266265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M.,
RA   Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A.,
RA   Marx C.J., Parnell J.J., Ramette A., Richardson P., Seeger M.,
RA   Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B.,
RA   Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp
RT   genome shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; CP000271; ABE33660.1; -; Genomic_DNA.
DR   RefSeq; YP_553010.1; -.
DR   GeneID; 4007176; -.
DR   GenomeReviews; CP000271_GR; Bxeno_B0692.
DR   KEGG; bxe:Bxe_B2328; -.
DR   HOGENOM; Q13QH9; -.
DR   OMA; Q13QH9; PLNPEWD.
DR   BioCyc; BXEN266265:BXE_B2328-MON; -.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    316       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase.
FT                                /FTId=PRO_0000337646.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   316 AA;  33575 MW;  F68D80644387A870 CRC64;
     MPVLLECLSH TPLSGYCDPA PEVVAEVGRI HAAARARVAD FDPQLVVVFA PDHYNGFFYD
     VMPPFCIGAS ASAVGDFGSL AGQLAVPADL ALDLAQAVLA ADVDVALSYR MQVDHGCAQA
     LEILTGSLDR YPVVPVFINS VAPPMASCRR ARLLGDALGR SLARSGQRVL VIGSGGISHE
     PPVPELAGAT DEVAERLIAG RNPSREARAA RQARTVAAAR AFAAGDSRLH PLNPEWDRAF
     LKLLEEGQLP ALDGFTDSAI TREAGKSAHE VRTWIAAFGA LQAYGPYRAT LDYYRPIPEW
     IAGFGAMHAR PIAETA
//