Q13QH9 (MHPB_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	Bxeno_B0692
ORF Names:	Bxe_B2328

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337646
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13QH9 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F68D80644387A870	FASTA	316	33,575
	10 20 30 40 50 60 MPVLLECLSH TPLSGYCDPA PEVVAEVGRI HAAARARVAD FDPQLVVVFA PDHYNGFFYD 70 80 90 100 110 120 VMPPFCIGAS ASAVGDFGSL AGQLAVPADL ALDLAQAVLA ADVDVALSYR MQVDHGCAQA 130 140 150 160 170 180 LEILTGSLDR YPVVPVFINS VAPPMASCRR ARLLGDALGR SLARSGQRVL VIGSGGISHE 190 200 210 220 230 240 PPVPELAGAT DEVAERLIAG RNPSREARAA RQARTVAAAR AFAAGDSRLH PLNPEWDRAF 250 260 270 280 290 300 LKLLEEGQLP ALDGFTDSAI TREAGKSAHE VRTWIAAFGA LQAYGPYRAT LDYYRPIPEW 310 IAGFGAMHAR PIAETA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000271 Genomic DNA. Translation: ABE33660.1.
RefSeq	YP_553010.1. NC_007952.1.
3D structure databases
ProteinModelPortal	Q13QH9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13QH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4007176.
GenomeReviews	Gene locus Bxeno_B0692 in contig CP000271_GR.
KEGG	bxe:Bxe_B2328.
PATRIC	19336230. VBIBurXen52548_5384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG565586.
OMA	AHEIRTW.
ProtClustDB	PRK13370.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B2328-MONOMER.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR023789. DHPP/DHXA_dioxygenase. IPR004183. Xdiol_dOase_suB. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KO	K05713.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
SUPFAM	SSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPB_BURXL

Accession

Primary (citable) accession number: Q13QH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program