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Protein

2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase

Gene

mhpC

Organism
Paraburkholderia xenovorans (strain LB400)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid.UniRule annotation

Catalytic activityi

(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate.UniRule annotation
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate.UniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Transition state stabilizerUniRule annotation1
Sitei194Catalytic role in ketonization of the dienol substrate (substrate destabilization)UniRule annotation1
Active sitei269Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00714.

Protein family/group databases

ESTHERiburxl-mhpc. Carbon-carbon_bond_hydrolase.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolaseUniRule annotation (EC:3.7.1.14UniRule annotation)
Alternative name(s):
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
Gene namesi
Name:mhpCUniRule annotation
Ordered Locus Names:Bxeno_B0697
ORF Names:Bxe_B2323
OrganismiParaburkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001817 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003377761 – 2892-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolaseAdd BLAST289

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi266265.Bxe_B2323.

Structurei

3D structure databases

ProteinModelPortaliQ13QH4.
SMRiQ13QH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 275AB hydrolase-1Sequence analysisAdd BLAST237

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MhpC family.Curated

Phylogenomic databases

eggNOGiENOG4106HB6. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028063.
KOiK05714.
OMAiGMIVQEM.
OrthoDBiPOG091H05R7.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01654. MhpC. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023791. MhpC_alpha/beta_hydrolase.
PfamiView protein in Pfam
PF00561. Abhydrolase_1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q13QH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSNVSAITE ASTSKFVRIK EGDGEVQLHY NDAGQGAETV VMLHGSGPGA
60 70 80 90 100
SGWANFNRNV EPLVAAGYRV ILLDCLGWSK SDPVVCDGSR SELNARSLKG
110 120 130 140 150
LLDALDIERV HIIGNSMGGH SAVAFALANP QRVGKLVLMG GGTGGPSQFV
160 170 180 190 200
PMPTEGIKLL QGLYREPTID NLKRMMNVFV FDASALTDDL MQARLDNMLA
210 220 230 240 250
RRDHLENFVK SLAANPKQFN DFGPRLGEIA APTLVIWGRD DRFVPMDVGL
260 270 280
RLVAGMQNAE MHIFSRCGHW AQWEHAEKFN RMVVDFLAH
Length:289
Mass (Da):31,587
Last modified:August 22, 2006 - v1
Checksum:i5EB5DE4809B1A090
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000271 Genomic DNA. Translation: ABE33665.1.
RefSeqiWP_011491025.1. NZ_CP008762.1.

Genome annotation databases

EnsemblBacteriaiABE33665; ABE33665; Bxe_B2323.
GeneIDi4007181.
KEGGibxb:DR64_4655.
bxe:Bxe_B2323.
PATRICifig|266265.5.peg.5389.

Similar proteinsi

Entry informationi

Entry nameiMHPC_PARXL
AccessioniPrimary (citable) accession number: Q13QH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: August 22, 2006
Last modified: June 7, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families