Reviewed, UniProtKB/Swiss-Prot Q13NG7 (BETA_BURXL)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Bxeno_B1404
ORF Names:	Bxe_B1592

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Choline dehydrogenase HAMAP MF_00750		PRO_0000258923
Regions
	Nucleotide binding	7 – 36	30	FAD Probable
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13NG7-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 1C0CDF3540761AD6	FASTA	561	62,136
	10 20 30 40 50 60 MAANEYDYII VGAGSAGNVL ASRLAEDADV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMNNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDGW AAHAGLENWT 130 140 150 160 170 180 YLDCLPYFRK AETRDIGANA YHGGDGPVHV TTSKPGNNPL FAAMVEAGVQ AGFPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTANGRR ASTARGYLDR AKARPNLTIV THAVTDRVLF SGKRAVGVSY 250 260 270 280 290 300 LHHGNVLNAQ ARREVLVCSG AIASPQLLQR SGVGRSTWLR ELDVPLVHDL PGVGENLQDH 310 320 330 340 350 360 LEMYIQYECK EPVSLYPALQ WWNQPAIGLE WMLNGTGIGA SNQFEAGGFI RTRDDDPWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIRM HGFQAHVGSM RSPSRGRVKL TSRDPNAHPG ILFNYMADPL 430 440 450 460 470 480 DWREFRDGIR ITREIMRQPA LDRYRGRELN PGAELTTDEQ LDSFVRMRAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD NEGRVHGMQG LRVVDASIMP RITTGNLNAP TIMLAEKIAD RIRGREALAR 550 560 VDTPYFVANG VASRKRESAT V

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000271 Genomic DNA. Translation: ABE34372.1.
RefSeq	YP_553722.1.
3D structure databases
SMR	Q13NG7. Positions 1-532.
ModBase	Search...
Genome annotation databases
GeneID	4007919.
GenomeReviews	Gene locus Bxeno_B1404 in contig CP000271_GR.
KEGG	bxe:Bxe_B1592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734713.
OMA	RTVTPHG.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B1592-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURXL

Accession

Primary (citable) accession number: Q13NG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)